Mest but Not MiR-335 Affects Skeletal Muscle Growth and Regeneration
Publication Date
June 22, 2015
Journal
PLOS ONE
Authors
Yosuke Hiramuki, Takahiko Sato, Yasuhide Furuta, M. Azim Surani, et al
Volume
10
Issue
6
Pages
e0130436
DOI
https://dx.plos.org/10.1371/journal.pone.0130436
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0130436
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26098312
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476715
Europe PMC
http://europepmc.org/abstract/MED/26098312
Web of Science
000356835800066
Scopus
84939198717
Mendeley
http://www.mendeley.com/research/mest-not-mir335-affects-skeletal-muscle-growth-regeneration
Events
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Mendeley | Further Information

{"title"=>"Mest but not MIR-335 affects skeletal muscle growth and regeneration", "type"=>"journal", "authors"=>[{"first_name"=>"Yosuke", "last_name"=>"Hiramuki", "scopus_author_id"=>"35484217500"}, {"first_name"=>"Takahiko", "last_name"=>"Sato", "scopus_author_id"=>"7406986325"}, {"first_name"=>"Yasuhide", "last_name"=>"Furuta", "scopus_author_id"=>"56358086500"}, {"first_name"=>"M. Azim", "last_name"=>"Surani", "scopus_author_id"=>"55665446900"}, {"first_name"=>"Atsuko", "last_name"=>"Sehara-Fujisawa", "scopus_author_id"=>"6603897255"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "sgr"=>"84939198717", "scopus"=>"2-s2.0-84939198717", "issn"=>"19326203", "pui"=>"605586216", "doi"=>"10.1371/journal.pone.0130436", "pmid"=>"26098312"}, "id"=>"93ba3394-8e22-3209-b9f4-dddfdfdbf848", "abstract"=>"When skeletal muscle fibers are injured, they regenerate and grow until their sizes are adjusted to surrounding muscle fibers and other relevant organs. In this study, we examined whether Mest, one of paternally expressed imprinted genes that regulates body size during development, and miR-335 located in the second intron of the Mest gene play roles in muscle regeneration. We generated miR-335-deficient mice, and found that miR-335 is a paternally expressed imprinted microRNA. Although both Mest and miR-335 are highly expressed during muscle development and regeneration, only Mest+/- (maternal/paternal) mice show retardation of body growth. In addition to reduced body weight in Mest+/-; DMD-null mice, decreased muscle growth was observed in Mest+/- mice during cardiotoxin-induced regeneration, suggesting roles of Mest in muscle regeneration. Moreover, expressions of H19 and Igf2r, maternally expressed imprinted genes were affected in tibialis anterior muscle of Mest+/-; DMD-null mice compared to DMD-null mice. Thus, Mest likely mediates muscle regeneration through regulation of imprinted gene networks in skeletal muscle.", "link"=>"http://www.mendeley.com/research/mest-not-mir335-affects-skeletal-muscle-growth-regeneration", "reader_count"=>23, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>6, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>6, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>13, "Medicine and Dentistry"=>1, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Japan"=>1, "Brazil"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2129460"], "description"=>"<p>(A) qRT-PCRs for paternally expressed (<i>Igf2</i>, <i>Peg3</i>, <i>Zac1</i>, <i>Dlk1</i>) and maternally expressed (<i>H19</i>, <i>Grb10</i>, <i>Igf2r</i>) imprinted genes were performed in TA muscles of 6 weeks old WT and <i>DMD-null</i> mice (n = 3 per genotype). (B) qRT-PCRs for <i>Igf2</i>, <i>H19</i>, and <i>Igf2r</i> mRNA were performed in TA muscles of 6 weeks old <i>DMD-null</i> (n = 4) and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> (n = 6). (C) qRT-PCRs for imprinted genes were performed with TA muscles obtained from WT mice from day 0 to day 10 after CTX injection (n = 3 per time point). (D) qRT-PCRs for imprinted genes were performed in TA muscles of 12–15 weeks old WT (n = 5) and <i>Mest</i><sup><i>+/-</i></sup> mice (n = 3) 6 days after CTX. Error bars indicate the s.e.m. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001.</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457104, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g005", "stats"=>{"downloads"=>5, "page_views"=>89, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_expression_level_of_imprinted_genes_is_altered_in_DMD_null_mice_and_CTX_injury_/1457104", "title"=>"The expression level of imprinted genes is altered in <i>DMD-null</i> mice and CTX injury.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129461"], "description"=>"<p>(A) Numbers of Pax7 positive-satellite cells per muscle fiber in sections of TA muscles of WT, <i>Mest</i><sup><i>+/-</i></sup>, and <i>miR-335</i><sup><i>+/Neo</i></sup> mice (n = 3 per genotype) at 10 weeks old in normal condition and 14 day after CTX. (B) qRT-PCRs for <i>Pax7</i> and <i>MyoD</i> mRNA were performed in normal TA muscles of 11–13 weeks old WT, <i>Mest</i><sup><i>+/-</i></sup>, and <i>miR-335</i><sup><i>+/Neo</i></sup> mice (n = 3 per genotype). Error bars indicate the s.e.m. *<i>P</i> < 0.05 compared with WT mice.</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457105, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g006", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Muscle_satellite_cells_are_not_affected_in_Mest__mice_/1457105", "title"=>"Muscle satellite cells are not affected in <i>Mest</i><sup><i>+/-</i></sup> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129462", "https://ndownloader.figshare.com/files/2129463", "https://ndownloader.figshare.com/files/2129464", "https://ndownloader.figshare.com/files/2129465"], "description"=>"<div><p>When skeletal muscle fibers are injured, they regenerate and grow until their sizes are adjusted to surrounding muscle fibers and other relevant organs. In this study, we examined whether <i>Mest</i>, one of paternally expressed imprinted genes that regulates body size during development, and miR-335 located in the second intron of the <i>Mest</i> gene play roles in muscle regeneration. We generated miR-335-deficient mice, and found that miR-335 is a paternally expressed imprinted microRNA. Although both <i>Mest</i> and miR-335 are highly expressed during muscle development and regeneration, only <i>Mest<sup>+/-</sup></i> (maternal/paternal) mice show retardation of body growth. In addition to reduced body weight in <i>Mest<sup>+/</sup>-; DMD-null</i> mice, decreased muscle growth was observed in <i>Mest<sup>+/-</sup></i> mice during cardiotoxin-induced regeneration, suggesting roles of Mest in muscle regeneration. Moreover, expressions of <i>H19</i> and <i>Igf2r</i>, maternally expressed imprinted genes were affected in tibialis anterior muscle of <i>Mest<sup>+/-</sup>; DMD-null</i> mice compared to <i>DMD-null</i> mice. Thus, Mest likely mediates muscle regeneration through regulation of imprinted gene networks in skeletal muscle.</p></div>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457106, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0130436.s001", "https://dx.doi.org/10.1371/journal.pone.0130436.s002", "https://dx.doi.org/10.1371/journal.pone.0130436.s003", "https://dx.doi.org/10.1371/journal.pone.0130436.s004"], "stats"=>{"downloads"=>11, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mest_but_Not_MiR_335_Affects_Skeletal_Muscle_Growth_and_Regeneration_/1457106", "title"=>"Mest but Not MiR-335 Affects Skeletal Muscle Growth and Regeneration", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129456"], "description"=>"<p>(A) qRT-PCRs for <i>Mest</i> mRNA and miR-335 were performed with TA muscles of P0, 6 weeks, and 12 weeks old WT mice (n = 3 per time point). (B) qRT-PCRs for <i>Mest</i> mRNA and miR-335 were performed with TA muscles of 3 months old WT (n = 4) and <i>DMD–null</i> mice (n = 7). (C) qRT-PCRs for <i>Mest</i> mRNA and miR-335 were performed with TA muscles from day 0 to day 10 after CTX injection (n = 3 per time point). (D) A schematic diagram of the Mest and miR-335 genomic region on chromosome 6 in mouse. (E) qRT-PCR for miR-335 was performed in TA muscles of WT and <i>Mest</i><sup><i>+/-</i></sup> mice (n = 3 per genotype). Expression of <i>Mest</i> and that of miR-335 are normalized to <i>Gapdh</i> and snoRNA-202, respectively. Error bars indicate the s.e.m. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001 compared with P0 (A), WT (B and E), and day 0 (C).</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457100, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g001", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mest_and_miR_335_are_coordinately_expressed_in_skeletal_muscle_during_postnatal_development_and_regeneration_/1457100", "title"=>"<i>Mest</i> and miR-335 are coordinately expressed in skeletal muscle during postnatal development and regeneration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129457"], "description"=>"<p>(A) Design of constructs used for generation of miR-335 deficient mice. The miR-335 genomic locus was replaced by a floxed neomycin-resistance cassette (loxP-Neo-loxP) to obtain <i>miR-335</i><sup><i>+/Neo</i></sup> mice. <i>miR-335</i><sup><i>+/-</i></sup> mice (+/-) were generated by crossing male <i>miR-335</i><sup><i>+/Neo</i></sup> mice with female CAG-<i>cre</i> transgenic mice. (B) Southern blot analysis of WT and G418 resistant ES clones with 5’ and 3’ probes. †: Non-specific band. (C and D) PCR analysis for targeted allele with genomic DNA in tails of WT (+/+), <i>miR-335</i><sup><i>+/Neo</i></sup> (+/Neo), and <i>miR-335</i><sup><i>+/-</i></sup> mice (+/-). In (C), an insertion and a deletion of a floxed neomycin-resistance cassette in genomic DNA of <i>miR-335</i><sup><i>+/Neo</i></sup> (+/Neo) and <i>miR-335</i><sup><i>+/-</i></sup> mice (+/-), respectively, were detected with PCRs amplified with primers shown in Fig 2A (Orange and Green arrows). In (D), a PCR analysis to distinguish alleles for WT (+/+), <i>miR-335</i><sup><i>+/-</i></sup> (+/-), and <i>miR-335</i><sup><i>-/-</i></sup> mice (-/-) was shown. The WT allele-specific (280 bp) and the mutant allele-specific (306 bp) bands were amplified with the primers shown in Fig 2A (Green arrow). (E, F and G) qRT-PCR for miR-335 was performed in TA muscles isolated from WT, <i>miR-335</i><sup><i>+/Neo</i></sup>, and <i>miR-335</i><sup><i>+/-</i></sup> or <i>miR-335</i><sup><i>-/+</i></sup> mice (n = 3 per genotype). (H and I) qRT-PCR for <i>Mest</i> mRNA was performed in TA muscles of WT, <i>miR-335</i><sup><i>+/-</i></sup>, and <i>miR-335</i><sup><i>+/Neo</i></sup> mice (n = 3 per genotype). Expression of <i>Mest</i> mRNA and that of miR-335 are normalized to <i>Gapdh</i> and snoRNA-202, respectively. Error bars indicate the s.e.m. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001.</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457101, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g002", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generation_of_miR_335_deficient_mice_/1457101", "title"=>"Generation of miR-335 deficient mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129458"], "description"=>"<p>(A) Representative images of 4 weeks old mice in individual genotypes. (B and C) Body weights of male littermate WT (n = 3–22), <i>Mest</i><sup><i>+/-</i></sup> (n = 6–15), <i>DMD-null</i> (n = 4–25), and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> mice (n = 4–22) from 1 to 12 (11–13) weeks old. (D) Body weights of WT (n = 15) and <i>miR-335</i><sup><i>+/Neo</i></sup> (n = 12) mice at 6 weeks. (E) Body weights of WT (n = 8–15) and <i>miR-335</i><sup><i>+/-</i></sup> mice (n = 15–18) from 1 to 6 weeks old. (F) TA muscle weights of male littermate WT (n = 13), <i>Mest</i><sup><i>+/-</i></sup> (n = 6), <i>DMD-null</i> (n = 18), and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> mice (n = 11) at 6 weeks old. (G) TA/Body weights of male littermate WT, <i>Mest</i><sup><i>+/-</i></sup>, <i>DMD-null</i>, and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> mice at 6 weeks old. (H) TA muscle weights of male littermate WT (n = 3), <i>Mest</i><sup><i>+/-</i></sup> (n = 6), <i>DMD-null</i> (n = 4), and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> mice (n = 4) at 11–13 weeks old. (I) TA/Body weights of male littermate WT, <i>Mest</i><sup><i>+/-</i></sup>, <i>DMD-null</i>, and <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> mice at 11–13 weeks old. (J and K) The numbers and average cross section areas of TA muscle fibers of male littermate WT (n = 7) and <i>Mest</i><sup><i>+/-</i></sup> mice (n = 4) at 6 weeks. Error bars indicate the s.e.m. *<i>P</i> < 0.05, ***<i>P</i> < 0.001. NS = Not significant.</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457102, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g003", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mest_is_required_for_body_and_skeletal_muscle_growth_/1457102", "title"=>"Mest is required for body and skeletal muscle growth.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2129459"], "description"=>"<p>(A) H&E staining of TA muscles under normal condition (top panel) and 14 days after CTX-induced injury (middle and bottom panels). Bottom panel shows the extended images of a part of middle panel. (B) Average cross section areas of TA muscles in WT (n = 3), <i>Mest</i><sup><i>+/-</i></sup> (n = 4) and <i>miR-335</i><sup><i>+/Neo</i></sup> mice (n = 6) under normal condition and WT (n = 7), <i>Mest</i><sup><i>+/-</i></sup> (n = 4) and <i>miR-335</i><sup><i>+/Neo</i></sup> mice (n = 6) 14 days after CTX injury. (C) H&E staining of TA muscles of <i>DMD-null</i>, <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> and <i>miR-335</i><sup><i>+/Neo</i></sup><i>; DMD-null</i> mice at 11–13 weeks old. (D) Average cross section areas of TA muscles in <i>DMD-null</i> (n = 8), <i>Mest</i><sup><i>+/-</i></sup><i>; DMD-null</i> (n = 4) and <i>miR-335</i><sup><i>+/Neo</i></sup><i>; DMD-null</i> mice (n = 4) at 11–13 weeks old. Error bars indicate the s.e.m. <sup><i>#</i></sup><i>P</i> = 0.0549 compared with WT mice. Scale bar: 100 μm.</p>", "links"=>[], "tags"=>["mir", "Igf 2r maternally", "mediates muscle regeneration", "mest", "Muscle fibers", "muscle regeneration"], "article_id"=>1457103, "categories"=>["Uncategorised"], "users"=>["Yosuke Hiramuki", "Takahiko Sato", "Yasuhide Furuta", "M. Azim Surani", "Atsuko Sehara-Fujisawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130436.g004", "stats"=>{"downloads"=>1, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mest_is_required_for_skeletal_muscle_growth_during_regeneration_/1457103", "title"=>"Mest is required for skeletal muscle growth during regeneration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-22 03:41:07"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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