Compartmentalized Cytokine Responses in Hidradenitis Suppurativa
Publication Date
June 19, 2015
Journal
PLOS ONE
Authors
Theodora Kanni, Vassiliki Tzanetakou, Athina Savva, Brigit Kersten, et al
Volume
10
Issue
6
Pages
e0130522
DOI
https://dx.plos.org/10.1371/journal.pone.0130522
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0130522
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26091259
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4474720
Europe PMC
http://europepmc.org/abstract/MED/26091259
Web of Science
000356835000124
Scopus
84939148194
Mendeley
http://www.mendeley.com/research/compartmentalized-cytokine-responses-hidradenitis-suppurativa
Events
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Mendeley | Further Information

{"title"=>"Compartmentalized cytokine responses in hidradenitis suppurativa", "type"=>"journal", "authors"=>[{"first_name"=>"Theodora", "last_name"=>"Kanni", "scopus_author_id"=>"26646679400"}, {"first_name"=>"Vassiliki", "last_name"=>"Tzanetakou", "scopus_author_id"=>"55580165000"}, {"first_name"=>"Athina", "last_name"=>"Savva", "scopus_author_id"=>"35079271200"}, {"first_name"=>"Brigit", "last_name"=>"Kersten", "scopus_author_id"=>"56091275400"}, {"first_name"=>"Aikaterini", "last_name"=>"Pistiki", "scopus_author_id"=>"35748924200"}, {"first_name"=>"Frank L.", "last_name"=>"Van De Veerdonk", "scopus_author_id"=>"6603439987"}, {"first_name"=>"Mihai G.", "last_name"=>"Netea", "scopus_author_id"=>"35378641700"}, {"first_name"=>"Jos W.", "last_name"=>"Van Der Meer", "scopus_author_id"=>"13402610900"}, {"first_name"=>"Evangelos J.", "last_name"=>"Giamarellos-Bourboulis", "scopus_author_id"=>"7005404293"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"10.1371/journal.pone.0130522", "sgr"=>"84939148194", "pui"=>"605585703", "scopus"=>"2-s2.0-84939148194", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0130522", "pmid"=>"26091259"}, "id"=>"b7186c2b-9ee8-3065-918d-7aee36b71c00", "abstract"=>"BACKGROUND: Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS).\\n\\nMETHODS: Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs) were stimulated for cytokine production; this was repeated in 13 severe patients during treatment with etanercept. Cytokines in pus were measured.\\n\\nRESULTS: CD14brightCD16dim inflammatory monocytes and patrolling monocytes were increased in Hurley III patients. Cytokine production by stimulated PBMCs was low compared to controls but the cytokine gene copies did not differ, indicating post-translational inhibition. The low production of IL-17 was restored, when cells were incubated with adalimumab. In pus, high concentrations of pro-inflammatory cytokines were detected. Based on the patterns, six different cytokine profiles were discerned, which are potentially relevant for the choice of treatment. Clinical improvement with etanercept was predicted by increased production of IL-1β and IL-17 by PBMCs at week 8.\\n\\nCONCLUSIONS: Findings indicate compartmentalized cytokine expression in HS; high in pus but suppressed in PBMCs. This is modulated through blockade of TNF.", "link"=>"http://www.mendeley.com/research/compartmentalized-cytokine-responses-hidradenitis-suppurativa", "reader_count"=>27, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Librarian"=>2, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Librarian"=>2, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>16, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>16}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"Brazil"=>1, "Germany"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2127134"], "description"=>"<p>Absolute counts of (A) total circulating monocytes, (B) CD14<sup>bright</sup>/CD16<sup>neg</sup> monocytes, (C) CD14<sup>bright</sup>/CD16<sup>dim</sup> monocytes, and (D) patrolling monocytes are shown for healthy volunteers (n = 14), patients with Hurley I HS (n = 37), with Hurley II HS (n = 37) and with Hurley III HS (n = 44). P values of comparisons by ANOVA and <i>post hoc</i> Bonferroni corrections: <sup>a</sup>0.004 vs healthy controls; <sup>b</sup>0.026 vs Hurley I; <sup>c</sup>0.001 vs healthy controls; <sup>d</sup>0.012 vs Hurley I; <sup>e</sup>0.008 vs Hurley II; <sup>f</sup>0.037 vs healthy controls.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455145, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Monocyte_subsets_in_HS_/1455145", "title"=>"Monocyte subsets in HS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127136"], "description"=>"<p>Production of (A) tumour necrosis factor-alpha (TNFα), (B) interleukin (IL)-1β, (C) IL-6, (D) IL-10, (E) IL-10, (F) IL-22, and (G) IL-1ra by peripheral blood mononuclear cells (PBMCs) of eight healthy controls and of 80 patients with hidradenitis suppurativa (HS). PBMCs were stimulated with LPS of <i>Escherichia coli</i> O55:B5; with heat-killed <i>Candida albicans</i> (HKCA), and with heat-killed <i>Staphylococcus aureus</i> (HKSA). (H) Gene transcripts of <i>TNF</i> and of <i>IL-1</i> after stimulation with LPS. P values refer to comparisons with healthy controls by the Mann-Whitney U test; pNS: difference not-significant.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455147, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stimulation_of_circulating_monocytes_/1455147", "title"=>"Stimulation of circulating monocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127137"], "description"=>"<p>Production of IL-10 and IL-17 was stimulated in peripheral blood mononuclear cells (PBMCs) of patients with hidradenitis suppurativa (HS) by LPS of <i>Escherichia coli</i> O55:B5, heat-killed <i>Candida albicans</i> (HKCA) and heat-killed <i>Staphylococcus aureus</i> (HKSA). Patients are divided into those with Hurley I or Hurley II disease stage (n = 50) and into those with Hurley III disease stage (n = 40). P values refer to comparisons between disease stages by the Mann Whitney U test; pNS: difference not-significant.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455148, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g003", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stimulation_of_IL_10_and_IL_17_in_relation_with_disease_severity_/1455148", "title"=>"Stimulation of IL-10 and IL-17 in relation with disease severity", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127138"], "description"=>"<p>Peripheral blood mononuclear cells (PBMCs) from of eight healthy controls and 40 patients with hidradenitis suppurativa were stimulated for the production of (A) interleukin (IL)-1β with LPS of <i>Escherichia coli</i> O55:B5; (B) IL-6 with LPS of <i>E</i>.<i>coli</i> O55:B5; (C) IL-10 with LPS of <i>E</i>.<i>coli</i> O55:B5; (D) IL-10 with heat-killed <i>Candida albicans</i> (HKCA); (E) IL-10 with heat-killed <i>Staphylococcus aureus</i> (HKSA); (F) IL-1ra with HKSA; (G) IL-17 with HKCA; (H) IL-17 with HKSA. P values refer to comparisons of cytokine production without/with adalimumab; pNS: difference not-significant.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455149, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g004", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Modulation_of_cytokine_release_by_adalimumab_/1455149", "title"=>"Modulation of cytokine release by adalimumab.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127140"], "description"=>"<p>(A) Concentrations of tumour necrosis factor-alpha (TNFα), interleukin (IL)-1β, IL-1α, IL-6, IL-10, IL-17 and Il-1ra were measured in the pus of the lesions of 28 patients of Hurley III disease stage. Lines represent medians. Correlations between (Β) the number of patrolling monocytes and pus TNFα; (C) the number of patrolling monocytes and pus IL-1β; and (D) IL-6 and years since disease onset. Classification patterns of patients according to pus cytokines is shown in panel E. Spearman’s coefficients of correlation and p values of significance are provided.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455151, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g005", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_concentrations_in_pus_of_patients_with_HS_/1455151", "title"=>"Cytokine concentrations in pus of patients with HS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127141"], "description"=>"<p>PBMCs were isolated and serum was sampled from patients with Hurley III disease at baseline and after 8 weeks of treatment with etanercept. Patients are divided into those experiencing worsening of symptoms (n = 6) or clinical improvement (n = 7) at the end of treatment. No change was defined when disease flare-ups persisted with heavy purulence. Improvement was defined as decrease of Sartorius score more than 30% from the baseline. PBMCs were stimulated with LPS of <i>Escherichia coli</i> O55:B5; with heat-killed <i>Candida albicans</i> (HKCA); and with heat-<i>Staphylococcus aureus</i> (HKSA) for the production of (A) tumour necrosis factor-alpha (TNFα), (B) interleukin (IL)-1β, (C) IL-17 and (D) C Reactive Protein (CRP). P values refer to comparisons of change of cytokine production from the baseline between improved and worsened cases by the Mann-Whitney U test.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455152, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g006", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_cytokine_production_and_of_C_Reactive_Protein_with_the_clinical_course_of_HS_/1455152", "title"=>"Changes of cytokine production and of C Reactive Protein with the clinical course of HS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127142"], "description"=>"<p>Patients with HS have monocytosis but cytokine responses of inflammatory monocytes and T17 lymphocytes are inhibited. This inhibition is mediated for T17 cells by tumour necrosis factor-alpha (TNFα). When cells migrate to tissues from the blood vessels through the vascular endothelium, capacity for excess production of TNFα, interleukin (IL)-1β, IL-1α, IL-6 and IL-17 is restored.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455153, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.g007", "stats"=>{"downloads"=>4, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_proposed_scheme_of_pathogenesis_of_HS_/1455153", "title"=>"A proposed scheme of pathogenesis of HS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127144"], "description"=>"<p>Clinical characteristics of patients enrolled in the study.</p>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455155, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522.t001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clinical_characteristics_of_patients_enrolled_in_the_study_/1455155", "title"=>"Clinical characteristics of patients enrolled in the study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-19 03:31:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/2127145"], "description"=>"<div><p>Background</p><p>Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS).</p><p>Methods</p><p>Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs) were stimulated for cytokine production; this was repeated in 13 severe patients during treatment with etanercept. Cytokines in pus were measured.</p><p>Results</p><p>CD14<sup>bright</sup>CD16<sup>dim</sup> inflammatory monocytes and patrolling monocytes were increased in Hurley III patients. Cytokine production by stimulated PBMCs was low compared to controls but the cytokine gene copies did not differ, indicating post-translational inhibition. The low production of IL-17 was restored, when cells were incubated with adalimumab. In pus, high concentrations of pro-inflammatory cytokines were detected. Based on the patterns, six different cytokine profiles were discerned, which are potentially relevant for the choice of treatment. Clinical improvement with etanercept was predicted by increased production of IL-1β and IL-17 by PBMCs at week 8.</p><p>Conclusions</p><p>Findings indicate compartmentalized cytokine expression in HS; high in pus but suppressed in PBMCs. This is modulated through blockade of TNF.</p></div>", "links"=>[], "tags"=>["cytokine gene copies", "cytokine profiles", "Hurley III patients", "hs", "week 8. ConclusionsFindings", "Hidradenitis Suppurativa BackgroundFavorable treatment outcomes", "120 patients", "pbmc", "hidradenitis suppurativa", "TNF blockade", "Compartmentalized Cytokine Responses", "cytokine responses", "monocyte", "flow cytometry", "cytokine production", "Clinical improvement", "il", "measured.ResultsCD 14brightCD", "compartmentalized cytokine expression", "Isolated blood"], "article_id"=>1455156, "categories"=>["Biological Sciences"], "users"=>["Theodora Kanni", "Vassiliki Tzanetakou", "Athina Savva", "Brigit Kersten", "Aikaterini Pistiki", "Frank L. van de Veerdonk", "Mihai G. Netea", "Jos W. van der Meer", "Evangelos J. Giamarellos-Bourboulis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130522", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Compartmentalized_Cytokine_Responses_in_Hidradenitis_Suppurativa_/1455156", "title"=>"Compartmentalized Cytokine Responses in Hidradenitis Suppurativa", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-19 03:31:21"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
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  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
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  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"28", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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