CD103+CD11b+ Dendritic Cells Induce Th17 T Cells in Muc2-Deficient Mice with Extensively Spread Colitis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"CD103+CD11b+ Dendritic Cells Induce Th17 T Cells in Muc2-Deficient Mice with Extensively Spread Colitis", "type"=>"journal", "authors"=>[{"first_name"=>"Ulf A.", "last_name"=>"Wenzel"}, {"first_name"=>"Caroline", "last_name"=>"Jonstrand"}, {"first_name"=>"Gunnar C.", "last_name"=>"Hansson"}, {"first_name"=>"Mary Jo", "last_name"=>"Wick"}], "year"=>2015, "source"=>"PLOS ONE", "identifiers"=>{"pmid"=>"26121642", "issn"=>"1932-6203", "doi"=>"10.1371/journal.pone.0130750"}, "id"=>"fa61a007-2f8a-36b2-b975-f64e64795094", "abstract"=>"Mucus alterations are a feature of ulcerative colitis (UC) and can drive inflammation by compromising the mucosal barrier to luminal bacteria. The exact pathogenesis of UC remains unclear, but CD4+ T cells reacting to commensal antigens appear to contribute to pathology. Given the unique capacity of dendritic cells (DCs) to activate naive T cells, colon DCs may activate pathogenic T cells and contribute to disease. Using Muc2-/- mice, which lack a functional mucus barrier and develop spontaneous colitis, we show that colitic animals have reduced colon CD103+ CD11b- DCs and increased CD103- CD11b+ phagocytes. Moreover, changes in colonic DC subsets and distinct cytokine patterns distinguish mice with distally localized colitis from mice with colitis spread proximally. Specifically, mice with proximally spread, but not distally contained, colitis have increased IL-1β, IL-6, IL-17, TNFα, and IFNγ combined with decreased IL-10 in the distal colon. These individuals also have increased numbers of CD103+ CD11b+ DCs in the distal colon. CD103+ CD11b+ DCs isolated from colitic but not noncolitic mice induced robust differentiation of Th17 cells but not Th1 cells ex vivo. In contrast, CD103- CD11b+ DCs from colitic Muc2-/- mice induced Th17 as well as Th1 differentiation. Thus, the local environment influences the capacity of intestinal DC subsets to induce T cell proliferation and differentiation, with CD103+ CD11b+ DCs inducing IL-17-producing T cells being a key feature of extensively spread colitis.", "link"=>"http://www.mendeley.com/research/cd103cd11b-dendritic-cells-induce-th17-t-cells-muc2deficient-mice-extensively-spread-colitis", "reader_count"=>17, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>8, "Medicine and Dentistry"=>3, "Neuroscience"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Neuroscience"=>{"Neuroscience"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84938709278"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84938709278?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938709278&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84938709278&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84938709278", "dc:identifier"=>"SCOPUS_ID:84938709278", "eid"=>"2-s2.0-84938709278", "dc:title"=>"CD103<sup>+</sup>CD11b<sup>+</sup> dendritic cells induce T<inf>h</inf>17 T cells in Muc2-deficient mice with extensively spread colitis", "dc:creator"=>"Wenzel U.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-06-29", "prism:coverDisplayDate"=>"29 June 2015", "prism:doi"=>"10.1371/journal.pone.0130750", "citedby-count"=>"14", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Göteborgs Universitet", "affiliation-city"=>"Gothenburg", "affiliation-country"=>"Sweden"}], "pubmed-id"=>"26121642", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0130750", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0130750", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"5", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"64", "xml_views"=>"4", "html_views"=>"183"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"29", "xml_views"=>"2", "html_views"=>"61"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2154265"], "description"=>"<p>P1, P2 and P3 cells were sorted from pooled MLN from Muc2<sup>+/-</sup>, Muc2<sup>-/-</sup> or colitic Muc2<sup>-/-</sup> and analyzed by flow cytometry. Sorted cells were pulsed with OVA<sub>(323–339)</sub> peptide prior to co-incubation with CTV-labeled OT-II cells for 5 days. (A) The gating strategy used to identify P1, P2, and P3 populations from MLN cell suspensions is shown. (B) Dot plots show CTV dilution of OT-II cells co-cultured with the indicated iMP subset pulsed with OVA<sub>(323–339)</sub> peptide. (C) Bar graphs show the frequency of proliferating OT-II T cells ± SD from (B).</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467230, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g005", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colitis_influences_the_ability_of_intestinal_DC_subsets_to_induce_CD4_T_cell_proliferation_/1467230", "title"=>"Colitis influences the ability of intestinal DC subsets to induce CD4<sup>+</sup> T cell proliferation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154262"], "description"=>"<p>Saponin extracts of proximal, middle and distal colon were prepared and normalized to total protein concentration. Cytokine expression was analyzed by CBA (A, C- F) or ELISA (B, G). Each symbol represents an individual mouse and the solid line indicates the mean of each group. Symbols of the same color in Figs 3 and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g004\" target=\"_blank\">4</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.s001\" target=\"_blank\">S1 Fig</a> represent samples from the same colon segment of the same animal. Mice are segregated into colitic and non-colitic according to neutrophil influx into the colon LP in the respective colon segment. Data are pooled from 5 independent experiments that examined 27 mice. The distal colon panels to the right of the vertical bar show the same distal colon data as to the left of the bar except that the colitic mouse group is segregated into group “A” and “B”. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467227, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inflammatory_cytokines_characterize_the_distal_colon_of_a_subgroup_of_colitic_Muc2_mice_/1467227", "title"=>"Inflammatory cytokines characterize the distal colon of a subgroup of colitic Muc2<sup>-/-</sup> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154269", "https://ndownloader.figshare.com/files/2154270"], "description"=>"<div><p>Mucus alterations are a feature of ulcerative colitis (UC) and can drive inflammation by compromising the mucosal barrier to luminal bacteria. The exact pathogenesis of UC remains unclear, but CD4<sup>+</sup> T cells reacting to commensal antigens appear to contribute to pathology. Given the unique capacity of dendritic cells (DCs) to activate naive T cells, colon DCs may activate pathogenic T cells and contribute to disease. Using Muc2<sup>-/-</sup> mice, which lack a functional mucus barrier and develop spontaneous colitis, we show that colitic animals have reduced colon CD103<sup>+</sup>CD11b<sup>-</sup> DCs and increased CD103<sup>-</sup>CD11b<sup>+</sup> phagocytes. Moreover, changes in colonic DC subsets and distinct cytokine patterns distinguish mice with distally localized colitis from mice with colitis spread proximally. Specifically, mice with proximally spread, but not distally contained, colitis have increased IL-1β, IL-6, IL-17, TNFα, and IFNγ combined with decreased IL-10 in the distal colon. These individuals also have increased numbers of CD103<sup>+</sup>CD11b<sup>+</sup> DCs in the distal colon. CD103<sup>+</sup>CD11b<sup>+</sup> DCs isolated from colitic but not noncolitic mice induced robust differentiation of Th17 cells but not Th1 cells <i>ex vivo</i>. In contrast, CD103<sup>-</sup>CD11b<sup>+</sup> DCs from colitic Muc2<sup>-/-</sup> mice induced Th17 as well as Th1 differentiation. Thus, the local environment influences the capacity of intestinal DC subsets to induce T cell proliferation and differentiation, with CD103<sup>+</sup>CD11b<sup>+</sup> DCs inducing IL-17-producing T cells being a key feature of extensively spread colitis.</p></div>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467234, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0130750.s001", "https://dx.doi.org/10.1371/journal.pone.0130750.s002"], "stats"=>{"downloads"=>3, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/CD103_CD11b_Dendritic_Cells_Induce_T_h_17_T_Cells_in_Muc2_Deficient_Mice_with_Extensively_Spread_Colitis/1467234", "title"=>"CD103<sup>+</sup>CD11b<sup>+</sup> Dendritic Cells Induce T<sub>h</sub>17 T Cells in Muc2-Deficient Mice with Extensively Spread Colitis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154263"], "description"=>"<p>LP cells of the other half of the same distal colon segment of the same mice used for cytokine analyses in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g003\" target=\"_blank\">Fig 3</a> were analyzed by flow cytometry. Cells were gated as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g002\" target=\"_blank\">Fig 2A</a>. (A-C) The frequency of DCs belonging to subsets P1 (A), P2 (B) or P3 (C) among viable MHCII<sup>+</sup>CD11c<sup>hi</sup> cells is shown. (D-E) The absolute number of DCs belonging to subsets P1 (D), P2 (E) or P3 (F) among 10<sup>6</sup> viable LP cells is depicted. Each symbol represents an individual mouse and the solid line indicates the mean of each group. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467228, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g004", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_profiles_in_the_distal_colon_of_colitic_mice_correlate_with_increased_CD103_CD11b_P2_DCs_in_the_same_colon_segment_/1467228", "title"=>"Cytokine profiles in the distal colon of colitic mice correlate with increased CD103<sup>+</sup>CD11b<sup>+</sup> P2 DCs in the same colon segment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154260"], "description"=>"<p>LP cells from proximal, middle and distal colon of Muc2<sup>+/-</sup> controls, Muc2<sup>-/-</sup>, and colitic Muc2<sup>-/-</sup> mice were analyzed by flow cytometry. (A) A representative dot plot of a Muc2<sup>+/-</sup> mouse showing gating of viable NK1.1<sup>-</sup>TCR<sup>-</sup>CD19<sup>-</sup>CD11c<sup>+</sup>MHC-II<sup>+</sup> cells is depicted in the left two dot plots and further analysis of gated CD11c<sup>+</sup>MHC-II<sup>+</sup> cells for expression of CD103 and CD11b for the indicated genotype of mouse is shown to the right. B-D depict the frequency of CD103<sup>+</sup>CD11b<sup>-</sup> \"P1\" DCs (B), CD103<sup>+</sup>CD11b<sup>+</sup> \"P2\" DCs (C), and CD103<sup>-</sup>CD11b<sup>+</sup> \"P3\" iMPs (D) among viable LP cells in the indicated gates. E-G show the absolute number of viable CD103<sup>+</sup>CD11b<sup>-</sup> \"P1\" DCs (E), CD103<sup>+</sup>CD11b<sup>+</sup> “P2” DCs (F) and CD103<sup>-</sup>CD11b<sup>+</sup> “P3” iMPs (G). Each symbol represents an individual mouse. For B-G results from the proximal (left column), middle (center column) and distal (right column) colon are shown. The mean of each group is indicated by the horizontal line. Segregating Muc2<sup>-/-</sup> mice into colitic and non-colitic is performed according to neutrophil influx in the respective colon segment. Data are from 12 independent experiments that analyzed 14–22 mice per group. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467225, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Altered_frequency_of_iMP_populations_in_the_colon_of_Muc2_mice_/1467225", "title"=>"Altered frequency of iMP populations in the colon of Muc2<sup>-/-</sup> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154258"], "description"=>"<p>LP cells from proximal, middle or distal colon sections of Muc2<sup>-/-</sup> and Muc2<sup>+/-</sup> littermates were analyzed by flow cytometry. (A) Viable neutrophils were identified as 7AAD<sup>-</sup>MHCII<sup>-</sup>CD11b<sup>+</sup>Ly6G<sup>+</sup> cells. The numbers represent the percent of cells in the indicated gate. (B) The frequency of neutrophils among viable LP cells for all mice examined is shown. The dashed line indicates the “cut off” value of 0.36%[<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.ref023\" target=\"_blank\">23</a>]). (C) The frequency of CD11b<sup>+</sup>MHC<sup>-/low</sup> cells among viable LP cells of all mice examined is depicted. Each symbol represents an individual mouse and the horizontal line indicates the mean. Results are from 12 independent experiments with a total of 14–22 mice per group. Statistical significance was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467223, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g001", "stats"=>{"downloads"=>2, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Local_accumulation_of_neutrophils_in_the_colon_of_Muc2_mice_/1467223", "title"=>"Local accumulation of neutrophils in the colon of Muc2<sup>-/-</sup> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154266"], "description"=>"<p>Cytokine concentrations in the supernatants of the T cell proliferation assays in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g005\" target=\"_blank\">Fig 5</a> were analyzed by Luminex and are shown in (A-C). (D-F) show the absolute number of differentiated CD4 T cells identified by staining for intracellular IFN-γ (left), CD25 and intracellular FoxP3 (middle) or intracellular IL-17A (right). Data are normalized to 10<sup>4</sup> CD4 T cells in co-cultures with the indicated DC subset after 5 days. Pooled data from 2 independent experiments in duplicates with a total of 6–8 animals/group is shown ± SD. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p>", "links"=>[], "tags"=>["Th 1 differentiation", "Th 1 cells", "Th 17 cells", "ifn", "uc", "Colitis Mucus alterations", "DC subsets", "colitis spread proximally", "T cell proliferation", "t cells", "tnf", "il"], "article_id"=>1467231, "categories"=>["Uncategorised"], "users"=>["Ulf A. Wenzel", "Caroline Jonstrand", "Gunnar C. Hansson", "Mary Jo Wick"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130750.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colitis_influences_CD4_T_cell_differentiation_induced_by_intestinal_DC_subsets_/1467231", "title"=>"Colitis influences CD4 T cell differentiation induced by intestinal DC subsets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:07:58"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"45", "full-text"=>"43", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"29", "full-text"=>"25", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"44", "full-text"=>"39", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"29", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"23", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"8", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts