MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells
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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2150076"], "description"=>"<p>Gastric cancer cells (SNU216 and SNU638) were transfected with MED30 siRNA (100 nM) or scrambled (SCR) siRNA. MED30 knockdown efficiency was determined by western blot (A) and real-time PCR (C) at 48 hr post-transfection. MED30 expression was also examined in cells stably overexpressing MED30 (MED30-over) and in empty control vector-transfected (Mock) cells. (B) MED30 protein levels relative to β-actin were quantified using Multi Gauge V3.1 software. Values indicate MED30 to β-actin band intensity ratios and are the means ± SDs of three independent experiments performed in triplicate. *, <i>p</i> < 0.01 (Student’s <i>t</i> test, versus SCR or Mock). (D) Effect of MED30 knockdown or overexpression on the proliferation of gastric cancer cells (SNU16, SNU216, SNU620, and SNU638). To check the effect of MED30 knockdown, five days after the transfection with 100 nM MED30 siRNA or SCR siRNA, cell viability assays were performed, and to examine the effect of MED30 overexpression, cell viability assays were performed after 3 days of incubation. Values are the means ± SDs of three independent experiments performed in quadruplicate. *, <i>p</i>< 0.01 (Student’s <i>t</i> test, versus SCR or Mock).</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464574, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g002", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED30_regulated_the_proliferation_of_gastric_cancer_cells_/1464574", "title"=>"MED30 regulated the proliferation of gastric cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150103"], "description"=>"<p>Cell invasion was examined using a Matrigel invasion assay. (A) Knockdown of MED30 significantly inhibited the FBS-induced invasions of SNU216 and SNU638 cells as compared with SCR siRNA. (B) MED30 overexpression (MED30-over) enhanced cell invasion compared with the mock control. (C) Invaded cells were counted and results are presented as a bar graph. Values are the means ± SDs of three independent experiments performed in triplicate. *, <i>p</i>< 0.01 (Student’s <i>t</i> test, versus SCR or Mock). Bar; 100 μm.</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464588, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED30_induced_the_invasion_of_gastric_cancer_cells_/1464588", "title"=>"MED30 induced the invasion of gastric cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150112"], "description"=>"<p>Correlation between the expression of MED30 and clinical classification in the gastric cancer.</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464597, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.t001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correlation_between_the_expression_of_MED30_and_clinical_classification_in_the_gastric_cancer_/1464597", "title"=>"Correlation between the expression of MED30 and clinical classification in the gastric cancer.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150111"], "description"=>"<p>Real-time PCR (A) and western blot analysis (B) of E-cadherin (CDH1), N-cadherin (CDH2), P-cadherin (CDH3), twist family bHLH transcription factor 1 (TWIST1/2), vimentin (VIM), and snail family zinc finger 1 and 2 (SNAI1/2) were performed after the knockdown or overexpression of MED30 in SNU638 cells. GAPDH and β-actin were used as internal control for real-time PCR and western blot analysis respectively. Values are the means ± SDs of three independent experiments performed in quadruplicate. *, <i>p</i>< 0.01, **<i>p</i>< 0.05 (Student’s <i>t</i> test, versus Mock). (C) SNU638 cells (MED30-over and mock) were grown in medium containing 10% FBS. After 2 days of culture, the morphologies of mock and MED30-overexpressing cells were observed by bright field microscopy.</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464596, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED30_induced_EMT_in_gastric_cancer_cells_/1464596", "title"=>"MED30 induced EMT in gastric cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150107"], "description"=>"<p>(A) SNU638 cells were transfected with MED30 or SCR siRNA and then injected subcutaneously into two sites per mouse. Tumor volumes were measured weekly from week three to seven post-injection. At week seven, mice were sacrificed and tumor volumes (B) and weights (C) were measured. Values are the means ± SDs of three independent experiments performed in triplicate. *, <i>p</i>< 0.01, **<i>p</i>< 0.05 (Student’s <i>t</i> test or one way ANOVA, versus SCR or Mock cells).</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464592, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_MED30_suppressed_tumor_growth_in_SCID_mice_/1464592", "title"=>"Knockdown of MED30 suppressed tumor growth in SCID mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150075"], "description"=>"<p>(A-D) Immunohistochemical staining showed the overexpression of MED30 in gastric cancer tissues (B-D) versus normal gastric mucosae (A). Its overexpression was observed in invading cancer cells (C) and metastatic cancer cells nearby lymph nodes (D) in addition to the primary cancer site (B). (E) The overexpression of MED30 in gastric cancer cells was determined by real-time PCR using specific primers (NT; normal tissue). GAPDH was used to normalize all data. Values are the means ± SDs of the three independent experiments performed in triplicate. *, <i>p</i>< 0.01 (Student’s <i>t</i> test, versus NT). (F) The overexpression of MED30 in gastric cancer tissues was examined by real-time PCR using specific primers. GAPDH was used to normalize all data. Values are the means ± SDs of the three independent experiments performed in triplicate. *, <i>p</i>< 0.01; **, <i>p</i>< 0.05 (Student’s <i>t</i> test, versus normal gastric tissues). (G) MED30 protein expression in higher N stages (N stages 2 and 3) was significantly greater than in lower N stages (N stage 0, 1) (N = 41, <i>p</i>< 0.05, Mann-Whitney U test).</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464573, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED30_was_overexpressed_in_gastric_cancer_tissues_/1464573", "title"=>"MED30 was overexpressed in gastric cancer tissues.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/2150090"], "description"=>"<p>Cell migration was evaluated using a Boyden chamber assay as described in ‘Materials and Methods’. (A) MED30 knockdown with MED30 siRNA significantly inhibited the FBS-induced migration of SNU216 and SNU638 cells compared with SCR siRNA. (B) MED30 overexpression (MED30-over) significantly increased migration compared to the mock control. (C) Migrated cells were counted and results are presented as a bar graph. Values are the means ± SDs of three independent experiments performed in triplicate. *, <i>p</i>< 0.01 (Student’s <i>t</i> test, versus SCR or Mock). Bar; 100 μm.</p>", "links"=>[], "tags"=>["cancer cells", "MED 30 overexpression", "MED 30 Regulates", "MED 30 knockdown", "RNA polymerase II", "Gastric Cancer Cells MED 30", "scid", "MED 30"], "article_id"=>1464581, "categories"=>["Uncategorised"], "users"=>["Yong Joo Lee", "Myoung-Eun Han", "Su-Jin Baek", "Seon-Young Kim", "Sae-Ock Oh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0130826.g003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED30_accelerated_the_migration_of_gastric_cancer_cells_/1464581", "title"=>"MED30 accelerated the migration of gastric cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-25 04:10:24"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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