Overexpression of Rat Neurons Nitric Oxide Synthase in Rice Enhances Drought and Salt Tolerance
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{"title"=>"Overexpression of rat neurons nitric oxide synthase in rice enhances drought and salt tolerance", "type"=>"journal", "authors"=>[{"first_name"=>"Wei", "last_name"=>"Cai", "scopus_author_id"=>"55574217819"}, {"first_name"=>"Wen", "last_name"=>"Liu", "scopus_author_id"=>"55003180000"}, {"first_name"=>"Wen Shu", "last_name"=>"Wang", "scopus_author_id"=>"56071702500"}, {"first_name"=>"Zheng Wei", "last_name"=>"Fu", "scopus_author_id"=>"55568854700"}, {"first_name"=>"Tong Tong", "last_name"=>"Han", "scopus_author_id"=>"56647427100"}, {"first_name"=>"Ying Tang", "last_name"=>"Lu", "scopus_author_id"=>"7405480849"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84938613243", "doi"=>"10.1371/journal.pone.0131599", "scopus"=>"2-s2.0-84938613243", "pui"=>"605481150", "issn"=>"19326203"}, "id"=>"a3041cdc-dae0-39d0-8cd3-41ab366351d6", "abstract"=>"Nitric oxide (NO) has been shown to play an important role in the plant response to biotic and abiotic stresses in Arabidopsis mutants with lower or higher levels of endogenous NO. The exogenous application of NO donors or scavengers has also suggested an important role for NO in plant defense against environmental stress. In this study, rice plants under drought and high salinity conditions showed increased nitric oxide synthase (NOS) activity and NO levels. Overexpression of rat neuronal NO synthase (nNOS) in rice increased both NOS activity and NO accumulation, resulting in improved tolerance of the transgenic plants to both drought and salt stresses. nNOS-overexpressing plants exhibited stronger water-holding capability, higher proline accumulation, less lipid peroxidation and reduced electrolyte leakage under drought and salt conditions than wild rice. Moreover, nNOS-overexpressing plants accumulated less H(2)O(2), due to the observed up-regulation of OsCATA, OsCATB and OsPOX1. In agreement, the activities of CAT and POX were higher in transgenic rice than wild type. Additionally, the expression of six tested stress-responsive genes including OsDREB2A, OsDREB2B, OsSNAC1, OsSNAC2, OsLEA3 and OsRD29A, in nNOS-overexpressing plants was higher than that in the wild type under drought and high salinity conditions. Taken together, our results suggest that nNOS overexpression suppresses the stress-enhanced electrolyte leakage, lipid peroxidation and H(2)O(2) accumulation, and promotes proline accumulation and the expression of stress-responsive genes under stress conditions, thereby promoting increased tolerance to drought and salt stresses.", "link"=>"http://www.mendeley.com/research/overexpression-rat-neurons-nitric-oxide-synthase-rice-enhances-drought-salt-tolerance", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>11, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Japan"=>1, "Russia"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2154745"], "description"=>"<p>(A) <i>nNOS</i> expression in the selected transgenic lines. The mRNA levels of <i>nNOS</i> in <i>nNOS</i>-overexpressing lines and the wild type were examined by quantitative RT–PCR analyses with <i>eEF1α</i> expression as the internal control. (B) NOS activities of the <i>nNOS</i>-overexpressing lines and wild-type plants were determined using a NOS assay kit. The relative NOS activity was expressed using the NOS activity of the wild type as the standard (1). (C and D) NO fluorescence in leaves of the <i>nNOS</i>-overexpressing lines and wild-type plants examined using DAF-FM DA (C) and the relative NO content (D) expressed using the fluorescence of the wild type as the standard (1). (E) NO contents of the leaves of both the <i>nNOS</i>-overexpressing lines and the wild type examined using an electrode-based method described in the Materials and Methods. The results shown are the mean ± SD. Values are derived from three independent biological experiments. ** (<i>P</i> < 0.01) indicates significant differences in comparison with wild-type control respectively (Student’s <i>t</i>-test).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467632, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_and_characterization_of_nNOS_overexpressing_lines_/1467632", "title"=>"Identification and characterization of <i>nNOS-</i>overexpressing lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154746"], "description"=>"<p>(A and B) Effect of mannitol and NaCl stresses on the endogenous NO level in the leaves of wild-type rice plants. Two-week-old wild-type plants were treated with either 200 mM mannitol or 200 mM NaCl combined with or without 500 μM L-NAME for 0, 1, 6 and 24 h, and then NO production was measured using DAF-FM DA. The relative NO content (A and B) was expressed using the fluorescence of the control at 0 h as the standard (1). (C and D) The NOS activity was determined using a NOS assay kit. The relative NOS activity (C and D) was expressed using the NOS activity of the control at 0 h as the standard (1). The results shown are the mean ± SD. Values are derived from three independent biological experiments. * (<i>P</i> < 0.05), ** (<i>P</i> < 0.01) and *** (<i>P</i> < 0.001) indicate significant differences in comparison with the water-treated control respectively (Student’s <i>t</i>-test).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467633, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g002", "stats"=>{"downloads"=>2, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Endogenous_NO_level_and_NOS_activity_in_rice_leaves_subjected_to_mannitol_and_NaCl_stresses_/1467633", "title"=>"Endogenous NO level and NOS activity in rice leaves subjected to mannitol and NaCl stresses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154753"], "description"=>"<p>(A and B) The appearance of 200 mM mannitol (A) or 200 mM NaCl (B) treated wild-type plants and <i>nNOS</i>-overexpressing lines at 10 d after germination. (C and D) Relative shoot length (C) and fresh weight (D) were assayed with both wild-type plants and three <i>nNOS</i>-overexpressing lines at 10 day after growth on the media (1/2 MS with or without 200 mM mannitol or 200 mM NaCl). The results shown are the mean ± SD. Values are derived from three independent biological experiments. (E and F) The appearance (E) and survival rates (F) of five-week-old wild-type and <i>nNOS</i>-overexpressing plants subjected to drought stress for 2 weeks and followed by re-watering for 7 d. The results shown are the mean ± SE (n = 3). * (<i>P</i> < 0.05), ** (<i>P</i> < 0.01) and *** (<i>P</i> < 0.001) indicate significant differences in comparison with wild-type control respectively (Student’s <i>t</i>-test).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467635, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g003", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_nNOS_overexpressing_lines_show_enhanced_drought_and_salt_tolerance_/1467635", "title"=>"The <i>nNOS-</i>overexpressing lines show enhanced drought and salt tolerance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154755"], "description"=>"<p>(A) Relative water content of wild-type and transgenic lines under mannitol and NaCl stresses. Relative water content (RWC) was assayed with both wild-type plants and <i>nNOS</i>-overexpressing lines at 10 day after growth on the media (1/2 MS with or without 200 mM mannitol or 200 mM NaCl). The results shown are the mean ± SD. (B) Water loss rate from the detached leaves of both wild-type plants and <i>nNOS</i>-overexpressing lines at indicated time points. The results shown are the mean ± SD. (C) Stomatal conductance was assayed on the flag leaves of both wild-type plants and <i>nNOS</i>-overexpressing lines. The results shown are the mean ± SD. (D-F) Two-week-old wild-type and <i>nNOS</i>-overexpressing plants were treated with either 200 mM mannitol or 200 mM NaCl for 2 days with water treatment as control, and then proline content (D), electrolyte leakage (E) and MDA content (F) were assayed. The results shown are the mean ± SE (n = 3). * (<i>P</i> < 0.05), ** (<i>P</i> < 0.01) and *** (<i>P</i> < 0.001) indicate significant differences in comparison with wild-type control respectively (Student’s <i>t</i>-test).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467637, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g004", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_physiological_parameters_of_both_nNOS_overexpressing_lines_and_wild_type_plants_under_drought_and_salt_stresses_/1467637", "title"=>"Changes of physiological parameters of both <i>nNOS</i>-overexpressing lines and wild-type plants under drought and salt stresses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154757"], "description"=>"<p>Two-week-old wild-type and <i>nNOS</i>-overexpressing plants were treated with either 200 mM mannitol or 200 mM NaCl for 2 days with water treatment as control, and then H<sub>2</sub>O<sub>2</sub> contents (A), the expression of <i>OsCATA</i> (B), <i>OsCATB</i> (C) and <i>OsPOX1</i> (D) and enzymatic activities of CAT (E) and POX (F) were assayed. The results shown are the mean ± SE (n = 3). * (<i>P</i> < 0.05) and ** (<i>P</i> < 0.01) indicate significant differences in comparison with wild-type control respectively (Student’s <i>t</i>-test).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467639, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g005", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_nNOS_overexpressing_lines_exhibit_improved_ROS_scavenging_capacity_under_drought_and_salt_stresses_/1467639", "title"=>"The <i>nNOS-</i>overexpressing lines exhibit improved ROS-scavenging capacity under drought and salt stresses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154759"], "description"=>"<p>(A-F) The expression of <i>OsDREB2A</i> (A), <i>OsDREB2B</i> (B), <i>OsSNAC1</i> (C), <i>OsSNAC2</i> (D), <i>OsLEA3</i> (E) and <i>OsRD29A</i> (F) in wild-type plants and <i>nNOS</i>-overexpressing lines under normal, drought and salt conditions was assayed by quantitative real-time PCR. <i>eEF1α</i> was used to normalize expression of these genes. The results shown are the mean ± SE (n = 3).</p>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467641, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599.g006", "stats"=>{"downloads"=>1, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_expression_of_stress_responsive_genes_in_nNOS_overexpressing_lines_subjected_to_drought_and_salt_stresses_/1467641", "title"=>"The expression of stress-responsive genes in <i>nNOS-</i>overexpressing lines subjected to drought and salt stresses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-29 03:37:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/2154760"], "description"=>"<div><p>Nitric oxide (NO) has been shown to play an important role in the plant response to biotic and abiotic stresses in Arabidopsis mutants with lower or higher levels of endogenous NO. The exogenous application of NO donors or scavengers has also suggested an important role for NO in plant defense against environmental stress. In this study, rice plants under drought and high salinity conditions showed increased nitric oxide synthase (NOS) activity and NO levels. Overexpression of rat neuronal NO synthase (<i>nNOS</i>) in rice increased both NOS activity and NO accumulation, resulting in improved tolerance of the transgenic plants to both drought and salt stresses. <i>nNOS</i>-overexpressing plants exhibited stronger water-holding capability, higher proline accumulation, less lipid peroxidation and reduced electrolyte leakage under drought and salt conditions than wild rice. Moreover, <i>nNOS</i>-overexpressing plants accumulated less H<sub>2</sub>O<sub>2</sub>, due to the observed up-regulation of <i>OsCATA</i>, <i>OsCATB</i> and <i>OsPOX1</i>. In agreement, the activities of CAT and POX were higher in transgenic rice than wild type. Additionally, the expression of six tested stress-responsive genes including <i>OsDREB2A</i>, <i>OsDREB2B</i>, <i>OsSNAC1</i>, <i>OsSNAC2</i>, <i>OsLEA3</i> and <i>OsRD29A</i>, in <i>nNOS</i>-overexpressing plants was higher than that in the wild type under drought and high salinity conditions. Taken together, our results suggest that <i>nNOS</i> overexpression suppresses the stress-enhanced electrolyte leakage, lipid peroxidation and H<sub>2</sub>O<sub>2</sub> accumulation, and promotes proline accumulation and the expression of stress-responsive genes under stress conditions, thereby promoting increased tolerance to drought and salt stresses.</p></div>", "links"=>[], "tags"=>["29a", "2a", "2b", "Salt Tolerance Nitric oxide", "Lipid peroxidation", "Nitric Oxide Synthase", "salinity conditions", "Rice Enhances Drought", "proline accumulation", "salt stresses", "nos", "H 2O accumulation", "pox", "Rat Neurons Nitric Oxide Synthase", "drought"], "article_id"=>1467642, "categories"=>["Uncategorised"], "users"=>["Wei Cai", "Wen Liu", "Wen-Shu Wang", "Zheng-Wei Fu", "Tong-Tong Han", "Ying-Tang Lu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0131599", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_Rat_Neurons_Nitric_Oxide_Synthase_in_Rice_Enhances_Drought_and_Salt_Tolerance_/1467642", "title"=>"Overexpression of Rat Neurons Nitric Oxide Synthase in Rice Enhances Drought and Salt Tolerance", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-29 03:37:54"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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