Identification of an Isothiocyanate on the HypEF Complex Suggests a Route for Efficient Cyanyl–Group Channeling during [NiFe]–Hydrogenase Cofactor Generation
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Identification of an isothiocyanate on the hypEF complex suggests a route for efficient cyanyl-group channeling during [NiFe]-Hydrogenase cofactor generation", "type"=>"journal", "authors"=>[{"first_name"=>"Sven T.", "last_name"=>"Stripp", "scopus_author_id"=>"25634933300"}, {"first_name"=>"Ute", "last_name"=>"Lindenstrauss", "scopus_author_id"=>"6507286105"}, {"first_name"=>"R. Gary", "last_name"=>"Sawers", "scopus_author_id"=>"8722768200"}, {"first_name"=>"Basem", "last_name"=>"Soboh", "scopus_author_id"=>"15729959200"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "sgr"=>"84941349045", "scopus"=>"2-s2.0-84941349045", "doi"=>"10.1371/journal.pone.0133118", "pui"=>"605952385"}, "id"=>"807ea263-0814-30de-822b-b54c19e9d06f", "abstract"=>"[NiFe]–hydrogenases catalyze uptake and evolution of H2 in a wide range of microorganisms. The enzyme is characterized by an inorganic nickel/ iron cofactor, the latter of which carries carbon monoxide and cyanide ligands. In vivo generation of these ligands requires a number of auxiliary proteins, the so–called Hyp family. Initially, HypF binds and activates the precursor metabolite carbamoyl phosphate. HypF catalyzes removal of phosphate and transfers the carbamate group to HypE. In an ATP–dependent condensation reaction, the C–terminal cysteinyl residue of HypE is modified to what has been interpreted as thiocyanate. This group is the direct precursor of the cyanide ligands of the [NiFe]–hydrogenase active site cofactor. We present a FT–IR analysis of HypE and HypF as isolated from E. coli. We follow the HypF–catalyzed cyanation of HypE in vitro and screen for the influence of carbamoyl phosphate and ATP. To elucidate on the differences between HypE and the HypEF complex, spectro–electrochemistry was used to map the vibrational Stark effect of naturally cyanated HypE. The IR signature of HypE could ultimately be assigned to isothiocyanate (–N=C=S) rather than thiocyanate (–S–C≡N). This has important implications for cyanyl–group channeling during [NiFe]–hydrogenase cofactor generation.", "link"=>"http://www.mendeley.com/research/identification-isothiocyanate-hypef-complex-suggests-route-efficient-cyanylgroup-channeling-during-n", "reader_count"=>8, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>2, "Chemistry"=>5}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84941349045"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84941349045?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84941349045&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84941349045&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84941349045", "dc:identifier"=>"SCOPUS_ID:84941349045", "eid"=>"2-s2.0-84941349045", "dc:title"=>"Identification of an isothiocyanate on the hypEF complex suggests a route for efficient cyanyl-group channeling during [NiFe]-Hydrogenase cofactor generation", "dc:creator"=>"Stripp S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-07-17", "prism:coverDisplayDate"=>"17 July 2015", "prism:doi"=>"10.1371/journal.pone.0133118", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Freie Universität Berlin", "affiliation-city"=>"Berlin", "affiliation-country"=>"Germany"}], "pubmed-id"=>"26186649", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0133118", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0133118", "share_count"=>1, "like_count"=>6, "comment_count"=>4, "click_count"=>0, "total_count"=>11}

Counter

  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"27", "xml_views"=>"6", "html_views"=>"120"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2181186"], "description"=>"<p>The αβ domains A and B are colored orange and green, respectively. The <i>C</i>–terminal loop region (330–338) is shown in blue. The loop ends with the conserved PR(V/I)C motif and the cysteine (C338) is modified to a thiocyanate in the published model [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref027\" target=\"_blank\">27</a>]. Inset: domains A and B form a surface cleft that localizes the C–terminus close to a magnesium–sequestered ATP molecule. Drawn after pdb coordinates 3WQJ.</p>", "links"=>[], "tags"=>["vibrational Stark effect", "NiFe", "HypEF Complex Suggests", "precursor metabolite carbamoyl phosphate", "cofactor", "ft", "atp", "cyanide ligands", "HypF catalyzes removal", "ir"], "article_id"=>1487438, "categories"=>["Biological Sciences"], "users"=>["Sven T. Stripp", "Ute Lindenstrauß", "R. Gary Sawers", "Basem Soboh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133118.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Crystal_structure_of_HypE_SCN_from_T_kodakarensis_in_the_inward_conformation_/1487438", "title"=>"Crystal structure of HypE–SCN from <i>T</i>. <i>kodakarensis</i> in the ‘inward’ conformation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 04:01:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/2181191"], "description"=>"<p>* In oxidized [FeFe]–hydrogenases, one CO ligand can be found in a ‘bridging’ position [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref048\" target=\"_blank\">48</a>]</p><p>(A) Tabulation of typical C–N stretching frequencies of thiocyanates, isothiocyanates, and isocyanates [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref042\" target=\"_blank\">42</a>,<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref043\" target=\"_blank\">43</a>,<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref046\" target=\"_blank\">46</a>,<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref047\" target=\"_blank\">47</a>]. (B) Iron cyanide and carbonyl stretching frequencies as found with mature [NiFe]–and [FeFe]–hydrogenases [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref004\" target=\"_blank\">4</a>].</p>", "links"=>[], "tags"=>["vibrational Stark effect", "NiFe", "HypEF Complex Suggests", "precursor metabolite carbamoyl phosphate", "cofactor", "ft", "atp", "cyanide ligands", "HypF catalyzes removal", "ir"], "article_id"=>1487443, "categories"=>["Biological Sciences"], "users"=>["Sven T. Stripp", "Ute Lindenstrauß", "R. Gary Sawers", "Basem Soboh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133118.t001", "stats"=>{"downloads"=>3, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Tabulation_of_typical_C_N_stretching_frequencies_of_thiocyanates_isothiocyanates_and_isocyanates_42_43_46_47_B_Iron_cyanide_and_carbonyl_stretching_frequencies_as_found_with_mature_NiFe_and_FeFe_hydrogenases_4_/1487443", "title"=>"(A) Tabulation of typical C–N stretching frequencies of thiocyanates, isothiocyanates, and isocyanates [42,43,46,47]. (B) Iron cyanide and carbonyl stretching frequencies as found with mature [NiFe]–and [FeFe]–hydrogenases [4].", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-17 04:01:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/2181189"], "description"=>"<p><b>(A)</b> Amide I (▲, 1658 cm<sup>–1</sup>) and amide II (●, 1549 cm<sup>–1</sup>) band formation over time after injection of HypE onto the bare gold surface. Additionally, the increase of the peak at 2118 cm<sup>–1</sup> (<b>□</b>) is followed. Kinetics are consistent with the Boltzmann model for a sigmoidal fit (R<sup>2</sup> as given in cursive brackets does not include the 18 h signal for 2118 cm<sup>–1</sup>). <b>(B)</b> SEIRAS spectrum of HypE from 2250 to 2000 cm<sup>–1</sup> without external potential including the thiocyanate vibration at 2118 cm<sup>–1</sup> (a). The peak fits best with contributions at 2119 and 2106 cm<sup>–1</sup>. Sequentially setting the cell potential to (b) +300 mV and (c) –300 mV vs. SHE gives rise to difference bands illustrating the vibrational Stark effect on HypE. Positive contributions are marked in bold. See text for details. Spectra (b) and (c) fit with three Gaussians to R = 8 x 10<sup>-6</sup> and 9 x 10<sup>-6</sup>, respectively.</p>", "links"=>[], "tags"=>["vibrational Stark effect", "NiFe", "HypEF Complex Suggests", "precursor metabolite carbamoyl phosphate", "cofactor", "ft", "atp", "cyanide ligands", "HypF catalyzes removal", "ir"], "article_id"=>1487441, "categories"=>["Biological Sciences"], "users"=>["Sven T. Stripp", "Ute Lindenstrauß", "R. Gary Sawers", "Basem Soboh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133118.g003", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SEIRAS_probes_the_vibrational_Stark_effect_of_HypE_/1487441", "title"=>"SEIRAS probes the vibrational Stark effect of HypE.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 04:01:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/2181190"], "description"=>"<p>The HypEF reaction mix was probed against a background of HypE alone. After t = 0, 20, and 30 minutes, a sharp peak appears at 2105 cm<sup>–1</sup> (FWHM ≈ 12 cm<sup>–1</sup>, grey box). The signal was found to decrease after 30 minutes. Incubation without carbamoyl phosphate (CP) did not result in peak formation (dashed line).</p>", "links"=>[], "tags"=>["vibrational Stark effect", "NiFe", "HypEF Complex Suggests", "precursor metabolite carbamoyl phosphate", "cofactor", "ft", "atp", "cyanide ligands", "HypF catalyzes removal", "ir"], "article_id"=>1487442, "categories"=>["Biological Sciences"], "users"=>["Sven T. Stripp", "Ute Lindenstrauß", "R. Gary Sawers", "Basem Soboh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133118.g004", "stats"=>{"downloads"=>0, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATR_FT_8211_IR_analysis_of_the_modification_of_HypE_by_HypF_and_carbamoyl_phosphate_/1487442", "title"=>"ATR FT–IR analysis of the modification of HypE by HypF and carbamoyl phosphate.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 04:01:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/2181187"], "description"=>"<p>Spectrum a) shows the rhodanide signature of HypE from <i>E</i>. <i>coli</i> at 2118 cm<sup>–1</sup>. Isolated from the same organism, spectrum b) shows the Fe(II)–(CN)<sub>2</sub>CO absorbance of HypD as discussed earlier [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref013\" target=\"_blank\">13</a>]. Spectrum c) shows the Ni–S active site signature of the regulatory hydrogenase from R. eutropha, ReRH [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133118#pone.0133118.ref033\" target=\"_blank\">33</a>]. The asterisk at 1961 cm<sup>–1</sup> indicates a minor fraction of Ni–C. Midpoint frequencies are given in bold wavenumbers, FWHM of the fitted Gaussian is in cursive brackets. The width of the grey boxes represents peak width and is plotted for illustration. All samples were probed with a spectral resolution of 4 cm<sup>–1</sup>.</p>", "links"=>[], "tags"=>["vibrational Stark effect", "NiFe", "HypEF Complex Suggests", "precursor metabolite carbamoyl phosphate", "cofactor", "ft", "atp", "cyanide ligands", "HypF catalyzes removal", "ir"], "article_id"=>1487439, "categories"=>["Biological Sciences"], "users"=>["Sven T. Stripp", "Ute Lindenstrauß", "R. Gary Sawers", "Basem Soboh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133118.g002", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATR_FT_8211_IR_analysis_of_HypE_HypD_and_ReRH_/1487439", "title"=>"ATR FT–IR analysis of HypE, HypD, and ReRH.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-17 04:01:36"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts