Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
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{"title"=>"Remodeling of tight junctions and enhancement of barrier integrity of the CACO-2 intestinal epithelial cell layer by micronutrients", "type"=>"journal", "authors"=>[{"first_name"=>"Mary Carmen", "last_name"=>"Valenzano", "scopus_author_id"=>"36794045600"}, {"first_name"=>"Katherine", "last_name"=>"DiGuilio", "scopus_author_id"=>"56730950600"}, {"first_name"=>"Joanna", "last_name"=>"Mercado", "scopus_author_id"=>"55334952300"}, {"first_name"=>"Mimi", "last_name"=>"Teter", "scopus_author_id"=>"57057414100"}, {"first_name"=>"Julie", "last_name"=>"To", "scopus_author_id"=>"56857345400"}, {"first_name"=>"Brendan", "last_name"=>"Ferraro", "scopus_author_id"=>"56857385200"}, {"first_name"=>"Brittany", "last_name"=>"Mixson", "scopus_author_id"=>"56857618400"}, {"first_name"=>"Isabel", "last_name"=>"Manley", "scopus_author_id"=>"56857570300"}, {"first_name"=>"Valerissa", "last_name"=>"Baker", "scopus_author_id"=>"56857256600"}, {"first_name"=>"Beverley A.", "last_name"=>"Moore", "scopus_author_id"=>"56857123000"}, {"first_name"=>"Joshua", "last_name"=>"Wertheimer", "scopus_author_id"=>"56857311400"}, {"first_name"=>"James M.", "last_name"=>"Mullin", "scopus_author_id"=>"7102294657"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84941985215", "doi"=>"10.1371/journal.pone.0133926", "pui"=>"606057558", "pmid"=>"26226276", "scopus"=>"2-s2.0-84941985215", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"62e03b73-6ab6-3b5f-8c53-2df005aa3771", "abstract"=>"The micronutrients zinc, quercetin, butyrate, indole and berberine were evaluated for their ability to induce remodeling of epithelial tight junctions (TJs) and enhance barrier integrity in the CACO-2 gastrointestinal epithelial cell culture model. All five of these chemically very diverse micronutrients increased transepithelial electrical resistance (Rt) significantly, but only berberine also improved barrier integrity to the non-electrolyte D-mannitol. Increases of Rt as much as 200% of untreated controls were observed. Each of the five micronutrients also induced unique, signature-like changes in TJ protein composition, suggesting multiple pathways (and TJ arrangements) by which TJ barrier function can be enhanced. Decreases in abundance by as much as 90% were observed for claudin-2, and increases of over 300% could be seen for claudins -5 and -7. The exact effects of the micronutrients on barrier integrity and TJ protein composition were found to be highly dependent on the degree of differentiation of the cell layer at the time it was exposed to the micronutrient. The substratum to which the epithelial layer adheres was also found to regulate the response of the cell layer to the micronutrient. The implications of these findings for therapeutically decreasing morbidity in Inflammatory Bowel Disease are discussed.", "link"=>"http://www.mendeley.com/research/remodeling-tight-junctions-enhancement-barrier-integrity-caco2-intestinal-epithelial-cell-layer-micr", "reader_count"=>46, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>4, "Student > Master"=>14, "Other"=>2, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>4, "Student > Master"=>14, "Other"=>2, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Engineering"=>1, "Nursing and Health Professions"=>3, "Biochemistry, Genetics and Molecular Biology"=>3, "Medicine and Dentistry"=>10, "Agricultural and Biological Sciences"=>20, "Neuroscience"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>10}, "Neuroscience"=>{"Neuroscience"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>5}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2195167"], "description"=>"<p>1A: 7-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed in control medium (apical and basal-lateral compartments) or medium containing 50μM, 100μM, or 200μM berberine chloride 12–17 hrs prior to electrical measurements. Data shown represents the mean ± standard error of 9 cell layers per condition (3 experiments, 3 cell layers per experiment). 1B: 7-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed, as above, in control medium or medium containing 1μM, 10μM, or 100μM Nicotine 48 hrs prior to electrical measurements. Data shown represents the mean ± standard error of 6 cell layers per condition (2 experiments, 3 cell layers per experiment). 1C: 4-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed, as in A, in control medium or medium containing 0.5mM, 2.0mM, or 5mM sodium butyrate 72 hrs prior to electrical measurements. Data shown represents the mean ± standard error of 6 cell layers per condition (2 experiments, 3 cell layers per experiment). 1D: 7-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed, as in A, in control medium or medium containing 100μM, 200μM, or 400μM quercetin 48hrs prior to electrical measurements. Data shown represents the mean ± standard error of 6 cell layers per condition (2 experiments, 3 cell layers per experiment). 1E: 7-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed, as in A, in control medium or medium containing 0.5mM, 1.0mM, or 2.0mM indole 48hrs prior to electrical measurements. Data shown represents the mean ± standard error of 9 cell layers per condition (3 experiments, 3 cell layers per experiment). 1F: 7-Day post-confluent CACO-2 cell layers on Millipore PCF filters were refed, as in A, in control medium or medium containing 50μM, 100μM, or 150μM zinc sulfate 48hrs prior to electrical measurements. Data shown represents the mean ± standard error of 12 cell layers for both the control and 100μM conditions, and 4 cell layers for both the 50μM and 150μM conditions. In all cases, data represents the percent of control resistance normalized for each experiment. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus control).</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498127, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_six_micronutrients_on_CACO_2_transepithelial_electrical_resistance_/1498127", "title"=>"The effect of six micronutrients on CACO-2 transepithelial electrical resistance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195168"], "description"=>"<p>In all cases, after electrical measurements, the same CACO-2 cell layers represented in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.g001\" target=\"_blank\">Fig 1</a> were used to perform radiotracer flux studies with 0.1mM, 0.20 μCi/ml <sup>14</sup>C-D-mannitol, as described in Materials and Methods. Data represents the percent of control flux rate normalized for each experiment, and is expressed as the mean ± standard error for the total number of cell layers per condition, as detailed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.g001\" target=\"_blank\">Fig 1</a>. * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus control).</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498128, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g002", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_six_micronutrients_on_CACO_2_transepithelial_flux_of_14_C_D_Mannitol_/1498128", "title"=>"The effect of six micronutrients on CACO-2 transepithelial flux of <sup>14</sup>C-D-Mannitol.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195170"], "description"=>"<p>The relative changes in the abundance of eight tight junctional proteins occurring as a function of days post confluence of the cell layer. 3-day, 7-day, and 21-day post-confluent CACO-2 cell layers in Falcon 75 cm<sup>2</sup> culture flasks, having been refed with control medium at confluence and every 2–3 days thereafter with control medium, were harvested in lysis buffer. Further steps were performed as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.t001\" target=\"_blank\">Table 1</a>. Data represents the percent of 3-day cell layers, and is expressed as the mean ± standard error for an n = 3 cell layers in all cases. NS indicates non significance. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus day 3).</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498130, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Change_of_tight_junctional_proteins_as_a_function_of_state_of_differentiation_of_the_CACO_2_cell_layer_/1498130", "title"=>"Change of tight junctional proteins as a function of state of differentiation of the CACO-2 cell layer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195171"], "description"=>"<p>3-day, 7-day, and 21-day CACO-2 cell layers were treated on the apical and basal-lateral sides with 100μM zinc for 48 hrs before electrical measurements. Data represents the percentage of normalized control resistance (2 experiments, 4 cell layers per condition per experiment) as a function of zinc treatment. Data shown is expressed as the mean ± standard error of 8 cell layers per condition. ** indicates P < 0.01 (21-day vs. 3-day); *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus day 3).</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498131, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g004", "stats"=>{"downloads"=>3, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_zinc_on_CACO_2_transepithelial_electrical_resistance_as_a_function_of_the_differentiation_state_of_the_CACO_2_cell_layer_/1498131", "title"=>"Effect of zinc on CACO-2 transepithelial electrical resistance as a function of the differentiation state of the CACO-2 cell layer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195172"], "description"=>"<p>3-day, 7-day, and 21-day post-confluent CACO-2 cell layers in Falcon 75 cm<sup>2</sup> culture flasks were refed with control medium or medium containing 100μM Zinc 48 hrs before harvesting in lysis buffer. Further steps were performed as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.t001\" target=\"_blank\">Table 1</a>. Data represents the percent of the zinc condition relative to the normalized control for each age cell layer. Data shown is expressed as the mean ± standard error for an n = 4 cell layers in all cases. NS indicates non significance. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (Student’s t test, two-tailed).</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498132, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g005", "stats"=>{"downloads"=>1, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_zinc_on_CACO_2_tight_junctional_proteins_as_a_function_of_the_differentiation_state_of_the_CACO_2_cell_layer_/1498132", "title"=>"Effect of zinc on CACO-2 tight junctional proteins as a function of the differentiation state of the CACO-2 cell layer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195173"], "description"=>"<p>1-day and 7-day post-confluent CACO-2 cell layers in Falcon 75 cm<sup>2</sup> culture flasks were refed with control medium or medium containing 400μM quercetin 48 hrs before harvesting in lysis buffer. Further steps were performed as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.t001\" target=\"_blank\">Table 1</a>. Data represents the percentage of band density of the no-quercetin control for that CACO-2 cell layer. Data shown is expressed as the mean ± standard error for an n = 3 cell layers in all cases. * indicates P < 0.05 for 1-day vs. 7-day changes in TJ protein (Student’s t test, two-tailed.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498133, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g006", "stats"=>{"downloads"=>2, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_effect_of_quercetin_treatment_on_the_tight_junctional_proteins_of_7_day_vs_1_day_post_confluent_CACO_2_cell_layers_/1498133", "title"=>"Differential effect of quercetin treatment on the tight junctional proteins of 7-day vs. 1-day post-confluent CACO-2 cell layers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195174"], "description"=>"<p>After 7 days, cell layers were refed in apical and basal-lateral compartments with control medium or medium containing 100μM zinc, 48 hrs before electrical measurements. Data represents the percentage of normalized control resistance, normalized control short circuit current, and normalized control flux rate (each relative to proper control; 2 experiments, 4 cell layers per condition). Data shown is expressed as the mean ± standard error of 8 cell layers per condition. P values (Student’s t test, two-tailed) are indicated for statistical comparisons of the various conditions.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498134, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.g007", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_the_cell_layer_substratum_on_CACO_2_epithelial_barrier_8217_s_response_to_zinc_CACO_2_cell_layers_were_seeded_at_identical_densities_on_Millicell_PCF_and_Millicell_HA_units_as_described_in_Materials_and_Methods_/1498134", "title"=>"The effect of the cell layer substratum on CACO-2 epithelial barrier’s response to zinc CACO-2 cell layers were seeded at identical densities on Millicell PCF and Millicell HA units as described in Materials and Methods.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195176"], "description"=>"<p>Summary of effects of various micronutrients on a panel of eight tight junctional proteins in CACO-2 cell layers. Post-confluent CACO-2 cell layers in Falcon 75 cm<sup>2</sup> culture flasks were refed on both sides with control medium or medium containing 100μM Zinc 48 hrs before harvesting in lysis buffer. These total cell lysates were analyzed by PAGE followed by immunoblotting as described in Methods. Immunoblots were probed with primary antisera against specific tight junctional antigens also as described. Densitometry was performed on developed blots to quantitate band densities. Data shown represents the mean ± standard error for an n = 3 cell layers in all cases. P values are listed for instances of statistical significance (Student’s t test [two tailed] to at least the P<0.05 level). NS indicates non significance. This same procedure was used for quercetin (400μM), butyrate (5.0mM), berberine (100μM), and indole (1.0mM) at the concentrations that provided maximal barrier enhancement and for the same time periods used in Figs <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.g001\" target=\"_blank\">1</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.g002\" target=\"_blank\">2</a>. Significant increases are highlighted in green while significant decreases are highlighted in yellow to emphasize the distinct overall pattern specific to each micronutrient.</p><p>Effects of Individual Micronutrients on CACO-2 Tight Junctional Proteins.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498136, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.t001", "stats"=>{"downloads"=>4, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_Individual_Micronutrients_on_CACO_2_Tight_Junctional_Proteins_/1498136", "title"=>"Effects of Individual Micronutrients on CACO-2 Tight Junctional Proteins.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195177"], "description"=>"<p>3-day, 7-day, and 21-day CACO-2 cell layers were refed in control medium the day after they were seeded onto Millicell PCFs, as well as, every two to three days until electrophysiology measurements and <sup>14</sup>C-D-mannitol flux studies were completed as described in Materials and Methods. Data shown for transepithelial resistance and short circuit current expressed as the mean ± standard error of 8 cell layers per condition (2 experiments, 4 cell layers per condition). Data shown for mannitol flux rate is expressed as the mean ± standard error of 4 cell layers per condition (1 experiment, 4 cell layers per condition).</p><p>* indicates P<0.05 (n = 4) vs. 3-day</p><p>** indicates P<0.01 (n = 8) vs. 3-day</p><p>*** indicates P < 0.001 vs 3-day (one-way ANOVA followed by Dunnett’s post hoc testing versus day 3 as control).</p><p>Note that SEM are 20% of means for all three parameters for 3-day cell layers but fall on average to only 7% for 7-day and 21-day cell layers, reflecting the relative cell layer heterogeneity inherent in 3-day cultures.</p><p>Effect of Days Post-Seeding on Transepithelial Parameters of CACO-2 Cell Layers.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498137, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.t002", "stats"=>{"downloads"=>8, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_Days_Post_Seeding_on_Transepithelial_Parameters_of_CACO_2_Cell_Layers_/1498137", "title"=>"Effect of Days Post-Seeding on Transepithelial Parameters of CACO-2 Cell Layers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195178"], "description"=>"<p>CACO-2 cell layers were seeded at identical densities on Millicell PCF and Millicell HA units as described in Materials and Methods. After 7 days, cell layers were refed in apical and basal-lateral compartments with control medium prior to electrical measurements and <sup>14</sup>C-D-mannitol flux studies. Data shown is expressed as the mean ± standard error of 8 cell layers per condition (2 experiments, 4 cell layers per condition).</p><p>NS indicates non significance.</p><p>*** indicates P<0.001 (Student’s t test, two-tailed).</p><p>Effect of the Filter Substratum on CACO-2 Transepithelial Barrier Function Properties.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498138, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.t003", "stats"=>{"downloads"=>10, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_Filter_Substratum_on_CACO_2_Transepithelial_Barrier_Function_Properties_/1498138", "title"=>"Effect of the Filter Substratum on CACO-2 Transepithelial Barrier Function Properties.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195179"], "description"=>"<p>* Indicates a statistically significant change</p><p>Points of Similarity and Dissimilarity in the Effects of Micronutrients on Barrier Function in the LLC-PK1 Renal Cell Layer vs the CACO-2 Gastrointestinal Cell Layer.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498139, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.t004", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Points_of_Similarity_and_Dissimilarity_in_the_Effects_of_Micronutrients_on_Barrier_Function_in_the_LLC_PK1_Renal_Cell_Layer_vs_the_CACO_2_Gastrointestinal_Cell_Layer_/1498139", "title"=>"Points of Similarity and Dissimilarity in the Effects of Micronutrients on Barrier Function in the LLC-PK1 Renal Cell Layer vs the CACO-2 Gastrointestinal Cell Layer.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195180"], "description"=>"<p>* Indicates a statistically significant change</p><p>Points of Similarity and Dissimilarity in Micronutrient Effects on Claudin Levels in the LLC-PK1 Renal Cell Layer vs the CACO-2 Gastrointestinal Cell Layer.</p>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498140, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0133926.t005", "stats"=>{"downloads"=>4, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Points_of_Similarity_and_Dissimilarity_in_Micronutrient_Effects_on_Claudin_Levels_in_the_LLC_PK1_Renal_Cell_Layer_vs_the_CACO_2_Gastrointestinal_Cell_Layer_/1498140", "title"=>"Points of Similarity and Dissimilarity in Micronutrient Effects on Claudin Levels in the LLC-PK1 Renal Cell Layer vs the CACO-2 Gastrointestinal Cell Layer.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-30 03:07:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/2195181", "https://ndownloader.figshare.com/files/2195182", "https://ndownloader.figshare.com/files/2195183", "https://ndownloader.figshare.com/files/2195184", "https://ndownloader.figshare.com/files/2195185", "https://ndownloader.figshare.com/files/2195186", "https://ndownloader.figshare.com/files/2195187", "https://ndownloader.figshare.com/files/2195188", "https://ndownloader.figshare.com/files/2195189", "https://ndownloader.figshare.com/files/2195190", "https://ndownloader.figshare.com/files/2195191", "https://ndownloader.figshare.com/files/2195192", "https://ndownloader.figshare.com/files/2195193"], "description"=>"<div><p>The micronutrients zinc, quercetin, butyrate, indole and berberine were evaluated for their ability to induce remodeling of epithelial tight junctions (TJs) and enhance barrier integrity in the CACO-2 gastrointestinal epithelial cell culture model. All five of these chemically very diverse micronutrients increased transepithelial electrical resistance (R<sub>t</sub>) significantly, but only berberine also improved barrier integrity to the non-electrolyte D-mannitol. Increases of R<sub>t</sub> as much as 200% of untreated controls were observed. Each of the five micronutrients also induced unique, signature-like changes in TJ protein composition, suggesting multiple pathways (and TJ arrangements) by which TJ barrier function can be enhanced. Decreases in abundance by as much as 90% were observed for claudin-2, and increases of over 300% could be seen for claudins -5 and -7. The exact effects of the micronutrients on barrier integrity and TJ protein composition were found to be highly dependent on the degree of differentiation of the cell layer at the time it was exposed to the micronutrient. The substratum to which the epithelial layer adheres was also found to regulate the response of the cell layer to the micronutrient. The implications of these findings for therapeutically decreasing morbidity in Inflammatory Bowel Disease are discussed.</p></div>", "links"=>[], "tags"=>["Inflammatory bowel disease", "epithelial cell culture model", "TJ barrier function", "barrier integrity", "micronutrient", "caco", "cell layer", "TJ protein composition"], "article_id"=>1498141, "categories"=>["Uncategorised"], "users"=>["Mary Carmen Valenzano", "Katherine DiGuilio", "Joanna Mercado", "Mimi Teter", "Julie To", "Brendan Ferraro", "Brittany Mixson", "Isabel Manley", "Valerissa Baker", "Beverley A. Moore", "Joshua Wertheimer", "James M. Mullin"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0133926.s001", "https://dx.doi.org/10.1371/journal.pone.0133926.s002", "https://dx.doi.org/10.1371/journal.pone.0133926.s003", "https://dx.doi.org/10.1371/journal.pone.0133926.s004", "https://dx.doi.org/10.1371/journal.pone.0133926.s005", "https://dx.doi.org/10.1371/journal.pone.0133926.s006", "https://dx.doi.org/10.1371/journal.pone.0133926.s007", "https://dx.doi.org/10.1371/journal.pone.0133926.s008", "https://dx.doi.org/10.1371/journal.pone.0133926.s009", "https://dx.doi.org/10.1371/journal.pone.0133926.s010", "https://dx.doi.org/10.1371/journal.pone.0133926.s011", "https://dx.doi.org/10.1371/journal.pone.0133926.s012", "https://dx.doi.org/10.1371/journal.pone.0133926.s013"], "stats"=>{"downloads"=>10, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Remodeling_of_Tight_Junctions_and_Enhancement_of_Barrier_Integrity_of_the_CACO_2_Intestinal_Epithelial_Cell_Layer_by_Micronutrients_/1498141", "title"=>"Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-07-30 03:07:13"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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