Similarities between the Binding Sites of SB-206553 at Serotonin Type 2 and Alpha7 Acetylcholine Nicotinic Receptors: Rationale for Its Polypharmacological Profile
Publication Date
August 05, 2015
Journal
PLOS ONE
Authors
Patricia Möller Acuña, J. Sebastián Contreras Riquelme, Cecilia Rojas Fuentes, Gabriel Nuñez Vivanco, et al
Volume
10
Issue
8
Pages
e0134444
DOI
https://dx.plos.org/10.1371/journal.pone.0134444
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0134444
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26244344
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4526571
Europe PMC
http://europepmc.org/abstract/MED/26244344
Web of Science
000359061400080
Scopus
84941978806
Mendeley
http://www.mendeley.com/research/similarities-between-binding-sites-sb206553-serotonin-type-2-alpha7-acetylcholine-nicotinic-receptor
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Mendeley | Further Information

{"title"=>"Similarities between the binding sites of SB-206553 at serotonin type 2 and alpha7 acetylcholine nicotinic receptors: Rationale for its polypharmacological profile", "type"=>"journal", "authors"=>[{"first_name"=>"Patricia", "last_name"=>"Möller-Acuña", "scopus_author_id"=>"56857620600"}, {"first_name"=>"J. Sebastián", "last_name"=>"Contreras-Riquelme", "scopus_author_id"=>"56857555900"}, {"first_name"=>"Cecilia", "last_name"=>"Rojas-Fuentes", "scopus_author_id"=>"56857532500"}, {"first_name"=>"Gabriel", "last_name"=>"Nuñez-Vivanco", "scopus_author_id"=>"56857632100"}, {"first_name"=>"Jans", "last_name"=>"Alzate-Morales", "scopus_author_id"=>"15829193100"}, {"first_name"=>"Patricio", "last_name"=>"Iturriaga-Vásquez", "scopus_author_id"=>"6507730880"}, {"first_name"=>"Hugo R.", "last_name"=>"Arias", "scopus_author_id"=>"7004514726"}, {"first_name"=>"Miguel", "last_name"=>"Reyes-Parada", "scopus_author_id"=>"6603477120"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84941978806", "issn"=>"19326203", "pui"=>"606057212", "pmid"=>"26244344", "doi"=>"10.1371/journal.pone.0134444", "sgr"=>"84941978806"}, "id"=>"7090c406-d154-3651-aeab-248a8e5c485c", "abstract"=>"Evidence from systems biology indicates that promiscuous drugs, i.e. those that act simultaneously at various protein targets, are clinically better in terms of efficacy, than those that act in a more selective fashion. This has generated a new trend in drug development called polypharmacology. However, the rational design of promiscuous compounds is a difficult task, particularly when the drugs are aimed to act at receptors with diverse structure, function and endogenous ligand. In the present work, using docking and molecular dynamics methodologies, we established the most probable binding sites of SB-206553, a drug originally described as a competitive antagonist of serotonin type 2B/2C metabotropic receptors (5-HT2B/2CRs) and more recently as a positive allosteric modulator of the ionotropic α7 nicotinic acetylcholine receptor (nAChR). To this end, we employed the crystal structures of the 5-HT2BR and acetylcholine binding protein as templates to build homology models of the 5-HT2CR and α7 nAChR, respectively. Then, using a statistical algorithm, the similarity between these binding sites was determined. Our analysis showed that the most plausible binding sites for SB-206553 at 5-HT2Rs and α7 nAChR are remarkably similar, both in size and chemical nature of the amino acid residues lining these pockets, thus providing a rationale to explain its affinity towards both receptor types. Finally, using a computational tool for multiple binding site alignment, we determined a consensus binding site, which should be useful for the rational design of novel compounds acting simultaneously at these two types of highly different protein targets.", "link"=>"http://www.mendeley.com/research/similarities-between-binding-sites-sb206553-serotonin-type-2-alpha7-acetylcholine-nicotinic-receptor", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>1, "Neuroscience"=>1, "Business, Management and Accounting"=>1, "Chemistry"=>2, "Psychology"=>2}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Psychology"=>{"Psychology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2202108"], "description"=>"<p>(A) Ribbon diagram of the superimposed structures of the 5-HT<sub>2B</sub>R (silver) and 5-HT<sub>2C</sub>R (purple), showing the putative binding site for SB-206553 (red or blue, respectively) at each protein. For comparative purposes, the binding site for ergotamine (yellow) in the crystal structure of the 5-HT<sub>2B</sub>R (PDB code 4IB4) is also depicted. (B-C) Close ups of the docking poses of SB-206553 at 5-HT<sub>2B</sub>R and 5-HT<sub>2C</sub>R, respectively. Main active site amino acid residues (cyan) are rendered as stick models.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503296, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g005", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Structural_determinants_of_the_SB_206553_binding_site_at_the_5_HT_2_Rs_/1503296", "title"=>"Structural determinants of the SB-206553 binding site at the 5-HT<sub>2</sub>Rs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202111"], "description"=>"<p>Ribbon diagram of the α7 nAChR model showing the putative binding sites for SB-206553 in the extracellular domain (ECD; red), at the M2-M3 loop (orange), and in the transmembrane domain (TMD; blue), respectively. β-Sheets and α-helices are shown in yellow and purple, respectively. The insets show molecular details of the docking poses of SB-206553 at each putative binding site. For comparative purposes, the binding pose of nicotine (green) in the crystal structure of the AChBP (PDB code 1UW6) is also depicted.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503299, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g006", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Potential_binding_sites_for_SB_206553_at_the_945_7_nAChR_/1503299", "title"=>"Potential binding sites for SB-206553 at the α7 nAChR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202117"], "description"=>"<p>RMSD values are shown for the compound when bound at: the extracellular domain (ECD; black line), the M2-M3 loop (red line), and the transmembrane domain (TMD; blue line).</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503305, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g007", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RMSD_behavior_of_SB_206553_docked_in_each_one_of_the_three_putative_binding_sites_from_the_945_7_nAChR_/1503305", "title"=>"RMSD behavior of SB-206553 docked in each one of the three putative binding sites from the α7 nAChR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202121"], "description"=>"<p>SB-206553 is shown in blue. Main active site amino acid residues (cyan) are rendered as stick models.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503309, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g008", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_mode_of_SB_206553_at_the_extracellular_domain_of_the_945_7_nAChR_/1503309", "title"=>"Binding mode of SB-206553 at the extracellular domain of the α7 nAChR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202123"], "description"=>"<p>Similarity profiles between the binding sites for SB-206553 docked at the 5-HT<sub>2B</sub>R and 5-HT<sub>2C</sub> R (A), at the 5-HT<sub>2B</sub>R and α7 nAChR (B), and at the 5-HT<sub>2B</sub>R and α7 nAChR (C), as calculated using PocketMatch. In each case, the horizontal black line indicates PMScore = 0.5. Each point corresponds to the PMScore.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503311, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g009", "stats"=>{"downloads"=>3, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Similarity_profiles_between_the_binding_sites_for_SB_206553_at_the_5_HT_2B_R_5_HT_2C_R_and_945_7_nAChR_/1503311", "title"=>"Similarity profiles between the binding sites for SB-206553 at the 5-HT<sub>2B</sub>R, 5-HT<sub>2C</sub>R, and α7 nAChR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202128"], "description"=>"<p>The cavity observed in the site is depicted as a transparent surface with residues in licorice format. Each color represents the chemical nature of residues (polar = green, non-polar = grey, negatively charged = red, positively charged = blue).</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503317, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g010", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Common_structure_of_the_SB_206553_binding_site_of_the_5_HT_2_Rs_and_945_7_nAChR_/1503317", "title"=>"Common structure of the SB-206553 binding site of the 5-HT<sub>2</sub>Rs and α7 nAChR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202098"], "description"=>"<p>Chemical structure of SB-206553.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503286, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g001", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chemical_structure_of_SB_206553_/1503286", "title"=>"Chemical structure of SB-206553.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202100"], "description"=>"<p>Conserved residues are highlighted in yellow and partially conserved residues highlighted in red. Secondary structures are shown schematically above the sequences; alpha helices and beta sheets are represented by cylinders and arrows respectively.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503288, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g002", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_the_extracellular_ECD_945_7_945_4_and_946_2_nAChR_subunits_and_AChBP_sequences_using_ClustalW_/1503288", "title"=>"Alignment of the extracellular (ECD) α7, α4 and β2 nAChR subunits and AChBP sequences using ClustalW.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202103"], "description"=>"<p>Conserved residues are highlighted in yellow and partially conserved residues are highlighted in red.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503291, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_the_transmembrane_TMD_7_4_and_2_nAChR_subunits_and_Torpedo_marmorata_nAChR_sequences_using_ClustalW_/1503291", "title"=>"Alignment of the transmembrane (TMD) α7, α4 and β2 nAChR subunits and <i>Torpedo marmorata</i> nAChR sequences using ClustalW.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202137", "https://ndownloader.figshare.com/files/2202138", "https://ndownloader.figshare.com/files/2202139", "https://ndownloader.figshare.com/files/2202140", "https://ndownloader.figshare.com/files/2202141", "https://ndownloader.figshare.com/files/2202142", "https://ndownloader.figshare.com/files/2202143", "https://ndownloader.figshare.com/files/2202144", "https://ndownloader.figshare.com/files/2202145"], "description"=>"<div><p>Evidence from systems biology indicates that promiscuous drugs, i.e. those that act simultaneously at various protein targets, are clinically better in terms of efficacy, than those that act in a more selective fashion. This has generated a new trend in drug development called polypharmacology. However, the rational design of promiscuous compounds is a difficult task, particularly when the drugs are aimed to act at receptors with diverse structure, function and endogenous ligand. In the present work, using docking and molecular dynamics methodologies, we established the most probable binding sites of SB-206553, a drug originally described as a competitive antagonist of serotonin type 2B/2C metabotropic receptors (5-HT<sub>2B/2C</sub>Rs) and more recently as a positive allosteric modulator of the ionotropic α7 nicotinic acetylcholine receptor (nAChR). To this end, we employed the crystal structures of the 5-HT<sub>2B</sub>R and acetylcholine binding protein as templates to build homology models of the 5-HT<sub>2C</sub>R and α7 nAChR, respectively. Then, using a statistical algorithm, the similarity between these binding sites was determined. Our analysis showed that the most plausible binding sites for SB-206553 at 5-HT<sub>2</sub>Rs and α7 nAChR are remarkably similar, both in size and chemical nature of the amino acid residues lining these pockets, thus providing a rationale to explain its affinity towards both receptor types. Finally, using a computational tool for multiple binding site alignment, we determined a consensus binding site, which should be useful for the rational design of novel compounds acting simultaneously at these two types of highly different protein targets.</p></div>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503325, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0134444.s001", "https://dx.doi.org/10.1371/journal.pone.0134444.s002", "https://dx.doi.org/10.1371/journal.pone.0134444.s003", "https://dx.doi.org/10.1371/journal.pone.0134444.s004", "https://dx.doi.org/10.1371/journal.pone.0134444.s005", "https://dx.doi.org/10.1371/journal.pone.0134444.s006", "https://dx.doi.org/10.1371/journal.pone.0134444.s007", "https://dx.doi.org/10.1371/journal.pone.0134444.s008", "https://dx.doi.org/10.1371/journal.pone.0134444.s009"], "stats"=>{"downloads"=>21, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Similarities_between_the_Binding_Sites_of_SB_206553_at_Serotonin_Type_2_and_Alpha7_Acetylcholine_Nicotinic_Receptors_Rationale_for_Its_Polypharmacological_Profile_/1503325", "title"=>"Similarities between the Binding Sites of SB-206553 at Serotonin Type 2 and Alpha7 Acetylcholine Nicotinic Receptors: Rationale for Its Polypharmacological Profile", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-08-05 03:48:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2202107"], "description"=>"<p>Conserved residues are highlighted in yellow.</p>", "links"=>[], "tags"=>["2r", "2b", "2CR", "nachr", "ht", "binding sites", "binding site alignment", "consensus binding site", "Serotonin Type 2", "receptor", "protein targets", "type", "Polypharmacological Profile Evidence", "sb", "acetylcholine binding protein", "2BR", "Alpha 7 Acetylcholine Nicotinic Receptors"], "article_id"=>1503295, "categories"=>["Biological Sciences"], "users"=>["Patricia Möller-Acuña", "J. Sebastián Contreras-Riquelme", "Cecilia Rojas-Fuentes", "Gabriel Nuñez-Vivanco", "Jans Alzate-Morales", "Patricio Iturriaga-Vásquez", "Hugo R. Arias", "Miguel Reyes-Parada"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134444.g004", "stats"=>{"downloads"=>4, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_5_HT_2B_R_and_5_HT_2C_R_sequences_using_ClustalW_/1503295", "title"=>"Alignment of 5-HT<sub>2B</sub>R and 5-HT<sub>2C</sub>R sequences using ClustalW.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-05 03:48:41"}

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  • {"unique-ip"=>"12", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"27", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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