Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition in Coculture by Controlling the Synthesis of HAQs
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{"title"=>"Pseudomonas aeruginosa DesB promotes staphylococcus aureus growth inhibition in coculture by controlling the synthesis of HAQs", "type"=>"journal", "authors"=>[{"first_name"=>"Sejeong", "last_name"=>"Kim", "scopus_author_id"=>"56571926800"}, {"first_name"=>"Yohan", "last_name"=>"Yoon", "scopus_author_id"=>"7402126663"}, {"first_name"=>"Kyoung Hee", "last_name"=>"Choi", "scopus_author_id"=>"8663377300"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84941978706", "sgr"=>"84941978706", "pui"=>"606057104", "isbn"=>"1932-6203", "pmid"=>"26230088", "doi"=>"10.1371/journal.pone.0134624"}, "id"=>"7444e435-a044-3c44-a429-553376558ce5", "abstract"=>"Pseudomonas aeruginosa is a pathogen that can cause serious infections and usually coexists with other pathogens, such as Staphylococcus aureus. Virulence factors are important for maintaining a presence of the organisms in these multispecies environments, and DesB plays an important role in P. aeruginosa virulence. Therefore, we investigated the effect of DesB on S. aureus reduction under competitive situation. Liquid cultures of P. aeruginosa wild type (WT) and its desB mutant were spotted on agar plates containing S. aureus, and the size of the clear zones was compared. In addition, interbacterial competition between P. aeruginosa and S. aureus was observed over time during planktonic coculture. The transcriptional profiles of the WT and desB mutant were compared by qRT-PCR and microarray to determine the role of DesB in S. aureus reduction at the molecular level. As a result, the clear zone was smaller for the desB mutant than for P. aeruginosa PAO1 (WT), and in planktonic coculture, the number of S. aureus cells was reduced in the desB mutant. qRT-PCR and microarray revealed that the expression of MvfR-controlled pqsA-E and phnAB operons was significantly decreased, but the mexEF-oprN operon was highly expressed. The results indicate that intracellular levels of 4-hydroxy-2-heptylquinoline (HHQ), a ligand of MvfR, are reduced due to MexEF-OprN-mediated efflux in desB mutant, resulting in the decrease of MvfR binding to pqsA-E promoter and the reduction of 4-hydroxy-2-alkylquinolines (HAQs) synthesis. Overexpression of mexEF-oprN operon in desB mutant was phenotypically confirmed by observing significantly increased resistance to chloramphenicol. In conclusion, these results suggest that DesB plays a role in the inhibition of S. aureus growth by controlling HAQ synthesis.", "link"=>"http://www.mendeley.com/research/pseudomonas-aeruginosa-desb-promotes-staphylococcus-aureus-growth-inhibition-coculture-controlling-s", "reader_count"=>11, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>6, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2197183"], "description"=>"<p><sup>1</sup> Fold change is reported as relative gene expression of <i>desB</i> mutant compared to WT (= 1).</p><p><sup>2</sup> The gene for more than 2-fold change in expression is bold.</p><p>Selected microarray analytical data to compare the expression of HAQ<i>-</i>related genes in WT and <i>desB</i> mutant.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499616, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.t003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Selected_microarray_analytical_data_to_compare_the_expression_of_HAQ_related_genes_in_WT_and_desB_mutant_/1499616", "title"=>"Selected microarray analytical data to compare the expression of HAQ<i>-</i>related genes in WT and <i>desB</i> mutant.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197181"], "description"=>"<p>Diagram of the metabolic pathway and possible mechanism related to the effect of <i>Pseudomonas aeruginosa</i> DesB on <i>Staphylococcus aureus</i> growth inhibition.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499614, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.g005", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Diagram_of_the_metabolic_pathway_and_possible_mechanism_related_to_the_effect_of_Pseudomonas_aeruginosa_DesB_on_Staphylococcus_aureus_growth_inhibition_/1499614", "title"=>"Diagram of the metabolic pathway and possible mechanism related to the effect of <i>Pseudomonas aeruginosa</i> DesB on <i>Staphylococcus aureus</i> growth inhibition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197177"], "description"=>"<p>Total RNA was extracted from 1 mL of the wild type (WT) and <i>desB</i> mutant (<i>desB</i>) <i>P</i>. <i>aeruginosa</i> PAO1 strains grown until an OD<sub>600</sub> of 0.4–0.5, and then cDNA was synthesized. The relative gene expression of <i>mvfR</i>, <i>pqsA</i>, <i>mexE</i>, <i>mexF</i>, <i>mexT</i>, and <i>oprN</i> in WT and <i>desB</i> was compared by qRT-PCR. The results are expressed as the fold-change of the relative gene expression in <i>desB</i> compared to that in WT.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499610, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_qRT_PCR_analysis_/1499610", "title"=>"qRT-PCR analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197186"], "description"=>"<div><p><i>Pseudomonas aeruginosa</i> is a pathogen that can cause serious infections and usually coexists with other pathogens, such as <i>Staphylococcus aureus</i>. Virulence factors are important for maintaining a presence of the organisms in these multispecies environments, and DesB plays an important role in <i>P</i>. <i>aeruginosa</i> virulence. Therefore, we investigated the effect of DesB on <i>S</i>. <i>aureus</i> reduction under competitive situation. Liquid cultures of <i>P</i>. <i>aeruginosa</i> wild type (WT) and its <i>desB</i> mutant were spotted on agar plates containing <i>S</i>. <i>aureus</i>, and the size of the clear zones was compared. In addition, interbacterial competition between <i>P</i>. <i>aeruginosa</i> and <i>S</i>. <i>aureus</i> was observed over time during planktonic coculture. The transcriptional profiles of the WT and <i>desB</i> mutant were compared by qRT-PCR and microarray to determine the role of DesB in <i>S</i>. <i>aureus</i> reduction at the molecular level. As a result, the clear zone was smaller for the <i>desB</i> mutant than for <i>P</i>. <i>aeruginosa</i> PAO1 (WT), and in planktonic coculture, the number of <i>S</i>. <i>aureus</i> cells was reduced in the <i>desB</i> mutant. qRT-PCR and microarray revealed that the expression of MvfR-controlled <i>pqsA-E</i> and <i>phnAB</i> operons was significantly decreased, but the <i>mexEF-oprN</i> operon was highly expressed. The results indicate that intracellular levels of 4-hydroxy-2-heptylquinoline (HHQ), a ligand of MvfR, are reduced due to MexEF-OprN-mediated efflux in <i>desB</i> mutant, resulting in the decrease of MvfR binding to <i>pqsA-E</i> promoter and the reduction of 4-hydroxy-2-alkylquinolines (HAQs) synthesis. Overexpression of <i>mexEF-oprN</i> operon in <i>desB</i> mutant was phenotypically confirmed by observing significantly increased resistance to chloramphenicol. In conclusion, these results suggest that DesB plays a role in the inhibition of <i>S</i>. <i>aureus</i> growth by controlling HAQ synthesis.</p></div>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499619, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624", "stats"=>{"downloads"=>4, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pseudomonas_aeruginosa_DesB_Promotes_Staphylococcus_aureus_Growth_Inhibition_in_Coculture_by_Controlling_the_Synthesis_of_HAQs/1499619", "title"=>"<i>Pseudomonas aeruginosa</i> DesB Promotes <i>Staphylococcus aureus</i> Growth Inhibition in Coculture by Controlling the Synthesis of HAQs", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197179"], "description"=>"<p>(A) WT and <i>desB</i> were exposed to chloramphenicol (0–512 μg/mL). After 24-h incubation, the optical density at 600 nm (OD<sub>600</sub>) was measured to determine bacterial growth in the presence of chloramphenicol. (B) Overnight cultures of WT and <i>desB</i> were 10-fold serially diluted and then spotted on chloramphenicol-containing agar plates (0, 8, 16, 32, 64, and 128 μg/mL). After incubation, growth was observed. Means with (*, **) are significantly different (<i>P</i> < 0.05) in WT or <i>desB</i> mutant.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499612, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.g004", "stats"=>{"downloads"=>3, "page_views"=>279, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chloramphenicol_resistance_of_Pseudomonas_aeruginosa_PAO1_wild_type_WT_and_desB_mutant_desB_strains_MIC_A_and_serial_dilution_spotting_assay_B_/1499612", "title"=>"Chloramphenicol resistance of <i>Pseudomonas aeruginosa</i> PAO1 wild type (WT) and <i>desB</i> mutant (<i>desB</i>) strains: MIC (A) and serial dilution-spotting assay (B).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197174"], "description"=>"<p>Overnight cultures of <i>Pseudomonas aeruginosa</i> PAO1 strains (wild type [1] and various mutants Δ<i>desT</i> [2], Δ<i>desA</i> [3], <i>desB</i> [4], Δ<i>fabA</i> [5], and Δ<i>fabA desB</i> [6]) were spotted onto an LB agar plate containing <i>Staphylococcus aureus</i> ATCC25923. After incubation at 37°C for 24 h, the diameter of the clear zone was measured.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499607, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spot_assay_for_Staphylococcus_aureus_reduction_/1499607", "title"=>"Spot assay for <i>Staphylococcus aureus</i> reduction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197175"], "description"=>"<p>Mixtures of <i>P</i>. <i>aeruginosa</i> (PA) wild type (WT) or <i>desB</i> mutant (<i>desB</i>) strains and <i>S</i>. <i>aureus</i> (SA) were spotted on a cellulose acetate filter disc and placed on an LB agar plate. During incubation, the growth of the individual bacterial species was analyzed by resuspending the filter disc in 0.5 mL of PBS and plating the suspension on selective agar. Means with (*) are significantly different (<i>P</i> < 0.05) in same timepoint.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499608, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Interspecies_growth_competition_assay_/1499608", "title"=>"Interspecies growth competition assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-07-31 03:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/2197182"], "description"=>"<p><sup>1</sup>Abbreviations: r, resistant; Tc, tetracycline; <i>FRT</i>, FLP recognition target.</p><p>Bacterial strains used in this study.</p>", "links"=>[], "tags"=>["wt", "aureus reduction", "aeruginosa PAO 1", "desb", "Pseudomonas aeruginosa DesB Promotes Staphylococcus aureus Growth Inhibition", "planktonic coculture", "operon", "HAQs Pseudomonas aeruginosa", "role", "hhq"], "article_id"=>1499615, "categories"=>["Uncategorised"], "users"=>["Sejeong Kim", "Yohan Yoon", "Kyoung-Hee Choi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0134624.t001", "stats"=>{"downloads"=>3, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bacterial_strains_used_in_this_study_/1499615", "title"=>"Bacterial strains used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-07-31 03:19:32"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
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  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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