Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Pennogenyl saponins from Paris quadrifolia L. induce extrinsic and intrinsic pathway of apoptosis in human cervical cancer HeLa cells", "type"=>"journal", "authors"=>[{"first_name"=>"Justyna", "last_name"=>"Stefanowicz-Hajduk", "scopus_author_id"=>"31067517200"}, {"first_name"=>"Rafal", "last_name"=>"Bartoszewski", "scopus_author_id"=>"15847599600"}, {"first_name"=>"Sylwia", "last_name"=>"Bartoszewska", "scopus_author_id"=>"36469714900"}, {"first_name"=>"Kinga", "last_name"=>"Kochan", "scopus_author_id"=>"55978287100"}, {"first_name"=>"Anna", "last_name"=>"Adamska", "scopus_author_id"=>"57062502800"}, {"first_name"=>"Igor", "last_name"=>"Kosiński", "scopus_author_id"=>"6507894952"}, {"first_name"=>"J. Renata", "last_name"=>"Ochocka", "scopus_author_id"=>"6701405345"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84943193468", "sgr"=>"84943193468", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0135993", "pmid"=>"26295969", "pui"=>"606195456"}, "id"=>"477c7839-ae6c-3ce9-ad8c-0679108a2144", "abstract"=>"Pennogenyl saponins are the active compounds of large number of plant species and consequently many polyherbal formulations. Hence, great interest has been shown in their characterization and in the investigation of their pharmacological and biological properties, especially anticancer. This present study reports on the evaluation of cytotoxic effects and explanation of the molecular mechanisms of action of the two pennogenyl saponins (PS 1 and PS 2) isolated from Paris quadrifolia L. rhizomes on human cervical adenocarcinoma cell line HeLa. To determine the viability of the cells treated with the compounds we used real-time cell proliferation analysis and found that the pennogenyl saponins PS 1 and PS 2 strongly inhibited the tumor cells growth with IC50 values of 1.11 ± 0.04 μg/ml and 0.87 ± 0.05 μg/ml, respectively. The flow cytometry analysis indicated that the two compounds induced apoptosis in a dose-dependent manner and decreased mitochondrial membrane potential in HeLa cells in the early stage of apoptosis. Quantitative PCR and Western Blot analysis showed that the two saponins significantly increased mRNA expression of FADD and BID as well as induced caspase-8 via increased of procaspase-8 processing in the treated cells. The results of this study suggest that both the extrinsic death receptor and intrinsic mitochondrial pathways are involved in the programmed cell death.", "link"=>"http://www.mendeley.com/research/pennogenyl-saponins-paris-quadrifolia-l-induce-extrinsic-intrinsic-pathway-apoptosis-human-cervical", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Librarian"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Librarian"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1, "Chemistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943193468"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84943193468?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84943193468&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84943193468&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84943193468", "dc:identifier"=>"SCOPUS_ID:84943193468", "eid"=>"2-s2.0-84943193468", "dc:title"=>"Pennogenyl saponins from Paris quadrifolia L. induce extrinsic and intrinsic pathway of apoptosis in human cervical cancer HeLa cells", "dc:creator"=>"Stefanowicz-Hajduk J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-08-21", "prism:coverDisplayDate"=>"21 August 2015", "prism:doi"=>"10.1371/journal.pone.0135993", "citedby-count"=>"9", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Gdanski Uniwersytet Medyczny", "affiliation-city"=>"Gdańsk", "affiliation-country"=>"Poland"}], "pubmed-id"=>"26295969", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0135993", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0135993", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"38", "xml_views"=>"4", "html_views"=>"189"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"97"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2220552"], "description"=>"<p>The cells were incubated with the pennogenyl saponins PS 1 (A) and PS 2 (B) at different concentrations for 24 h. The numeric labels on the curves represent the increasing concentration values of the compounds (0.1, 0.5, 1.0, 2.0, 5.0, 10.0 μg/ml, respectively).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516716, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pennogenyl_saponins_treatment_decreased_HeLa_cells_viability_/1516716", "title"=>"The pennogenyl saponins treatment decreased HeLa cells viability.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220560"], "description"=>"<p>The distribution of the cells undergoing loss of mitochondrial potential was determined after incubating the untreated cells (A), the cells with 1.0% DMSO (B), the compound PS 1 (C-E) and PS 2 (F-H) at a concentration of 1.0, 5.0, 10.0 μg/ml, respectively. The cells were treated for 3 h and the extent of mitochondrial cell depolarization was determined in comparison to the DMSO control (I). Each sample was run in triplicate. Error bars represent standard deviations. Significant differences relative to the control are marked with an “*” (p<0.05).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516725, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pennogenyl_saponins_induced_changes_in_transmembrane_mitochondrial_potential_in_HeLa_cells_as_determined_by_flow_cytometry_/1516725", "title"=>"Pennogenyl saponins-induced changes in transmembrane mitochondrial potential in HeLa cells as determined by flow cytometry.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220559"], "description"=>"<p>Lanes 1 and 1 a represent DNA of untreated cells; lanes 2, 2a – cells treated with 0.1% DMSO (ctrl); lane 3 – cells treated with the compound PS 1; lane 4—cells treated with the compound PS 2. The cells were incubated with the compounds at a concentration of 1 μg/ml for 48 h (the concentration value related to the IC50 values of the compounds). M–DNA ladder (Thermo Scientific).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516724, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g005", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_saponins_treatment_induced_DNA_fragmentation_in_HeLa_cells_/1516724", "title"=>"The saponins treatment induced DNA fragmentation in HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220555"], "description"=>"<p>The cells were analyzed by flow cytometry using annexin V-PE/7-AAD staining method. The distribution of the apoptotic cells was determined in the sample of untreated cells (A) and after incubating the cells with 0.5% DMSO (B), the compound PS 1 (C-E) and PS 2 (F-H) at a concentration of 0.5, 3.0 and 5.0 μg/ml, respectively. The cells were treated for 24 h and the total extent of apoptosis (apoptosis rate) was determined in comparison to the DMSO control (I). Each sample was run in triplicate. Error bars represent standard deviations. Significant differences relative to the control are marked with an “*” (p<0.05).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516720, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pennogenyl_saponins_induced_apoptosis_in_HeLa_cells_/1516720", "title"=>"The pennogenyl saponins induced apoptosis in HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220554"], "description"=>"<p>The IC50 values of the saponins were calculated based on the dose-response curves of the cell index by the xCELLigence system. Error bars represent the standard deviations.</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516718, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RTCA_measurement_of_CI_values_of_HeLa_cells_treated_with_the_compound_PS_1_A_and_PS_2_B_for_24_h_/1516718", "title"=>"RTCA measurement of CI values of HeLa cells treated with the compound PS 1 (A) and PS 2 (B) for 24 h.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220562"], "description"=>"<p>HeLa cells were incubated with the compound PS 1 and PS 2 at a concentration of 1.0 μg/ml (the concentration value related to the IC50 values of the saponins) for 5 h and the expression of proteins in the treated cells was determined by Western blot analysis. PS 1 and PS 2 decreased Bcl-2 (A) and procaspase-8 (55 kDa) (C) protein levels and increased Bid (B) protein expression in the cells. The experiments were repeated three times and each protein level was related to 0.1% DMSO control sample. Error bars represent standard deviations. Significant differences relative to the control are marked with an “*” (p<0.05).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516727, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g008", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pennogenyl_saponins_treatment_induced_changes_in_apoptosis_related_protein_expression_/1516727", "title"=>"The pennogenyl saponins treatment induced changes in apoptosis-related protein expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220563"], "description"=>"<p>The structure of <i>Paris</i> saponin PS 1 and PS 2.</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516728, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g009", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_structure_of_Paris_saponin_PS_1_and_PS_2_/1516728", "title"=>"The structure of <i>Paris</i> saponin PS 1 and PS 2.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220561"], "description"=>"<p>The cells were stimulated with the compounds PS 1 and PS 2 at a concentration of 1.0 and 0.5 μg/ml, respectively and incubated for 24 h. The mRNA levels were measured in real-time PCR experiments. Each sample was run in duplicate, and the relative amount of mRNA was normalized to the 18S rRNA content and expressed as a fold-change over the control (0.1% DMSO). Error bars represent standard deviations. Significant differences relative to the control are marked with an “*” (p<0.05).</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516726, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g007", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pennogenyl_compounds_treatment_induced_changes_in_mRNA_levels_of_the_apoptotic_genes_in_HeLa_cells_/1516726", "title"=>"The pennogenyl compounds treatment induced changes in mRNA levels of the apoptotic genes in HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/2220557"], "description"=>"<p>Nuclear chromatin state was assessed with Hoechst 33342 staining after exposure the cells to 1 μg/ml of the compound PS 1 (A) and PS 2 (B) for 24 h (the concentration value related to the IC50 values of the saponins). The cells treated with the saponins show condensed chromatin relative to the control cells incubated with 0.1% DMSO (C). Arrows represent apoptotic cells.</p>", "links"=>[], "tags"=>["fadd", "Western Blot analysis", "adenocarcinoma cell line HeLa", "pennogenyl saponins PS 1", "compound", "mRNA expression", "study reports", "tumor cells growth", "extrinsic death receptor", "Human Cervical Cancer HeLa Cells Pennogenyl saponins", "Quantitative PCR", "Intrinsic Pathway", "IC 50 values", "HeLa cells", "pennogenyl saponins", "cell proliferation analysis", "Paris quadrifolia L", "flow cytometry analysis", "Mitochondrial membrane", "plant species", "bid", "cytotoxic effects", "polyherbal formulations", "Induce Extrinsic", "Cell death", "PS 2", "mitochondrial pathways", "PS 1"], "article_id"=>1516722, "categories"=>["Biological Sciences"], "users"=>["Justyna Stefanowicz-Hajduk", "Rafal Bartoszewski", "Sylwia Bartoszewska", "Kinga Kochan", "Anna Adamska", "Igor Kosiński", "J. Renata Ochocka"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0135993.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pennogenyl_saponins_induced_apoptotic_changes_in_HeLa_cells_/1516722", "title"=>"The pennogenyl saponins induced apoptotic changes in HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-21 03:50:00"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"44", "full-text"=>"53", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"30", "full-text"=>"23", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts