Impaired Cell Cycle Regulation in a Natural Equine Model of Asthma
Publication Date
August 20, 2015
Journal
PLOS ONE
Authors
Alicja Pacholewska, Vidhya Jagannathan, Michaela Drögemüller, Jolanta Klukowska Rötzler, et al
Volume
10
Issue
8
Pages
e0136103
DOI
https://dx.plos.org/10.1371/journal.pone.0136103
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0136103
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26292153
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546272
Europe PMC
http://europepmc.org/abstract/MED/26292153
Web of Science
000359919900055
Scopus
84942941574
Mendeley
http://www.mendeley.com/research/impaired-cell-cycle-regulation-natural-equine-model-asthma-1
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Impaired cell cycle regulation in a natural equine model of asthma", "type"=>"journal", "authors"=>[{"first_name"=>"Alicja", "last_name"=>"Pacholewska", "scopus_author_id"=>"55964827200"}, {"first_name"=>"Vidhya", "last_name"=>"Jagannathan", "scopus_author_id"=>"7006778326"}, {"first_name"=>"Michaela", "last_name"=>"Drögemüller", "scopus_author_id"=>"6507424400"}, {"first_name"=>"Jolanta", "last_name"=>"Klukowska-Rötzler", "scopus_author_id"=>"8353286700"}, {"first_name"=>"Simone", "last_name"=>"Lanz", "scopus_author_id"=>"54407001600"}, {"first_name"=>"Eman", "last_name"=>"Hamza", "scopus_author_id"=>"23004596700"}, {"first_name"=>"Emmanouil T.", "last_name"=>"Dermitzakis", "scopus_author_id"=>"56571328200"}, {"first_name"=>"Eliane", "last_name"=>"Marti", "scopus_author_id"=>"7005623468"}, {"first_name"=>"Tosso", "last_name"=>"Leeb", "scopus_author_id"=>"7006758115"}, {"first_name"=>"Vincent", "last_name"=>"Gerber", "scopus_author_id"=>"7005969573"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"3100301382", "pmid"=>"26292153", "scopus"=>"2-s2.0-84942941574", "pui"=>"606195205", "doi"=>"10.1371/journal.pone.0136103", "sgr"=>"84942941574"}, "id"=>"b9d25fde-1d53-3ef8-8ca8-ab8a266ab936", "abstract"=>"Recurrent airway obstruction (RAO) is a common and potentially debilitating lower airway disease in horses, which shares many similarities with human asthma. In susceptible horses RAO exacerbation is caused by environmental allergens and irritants present in hay dust. The objective of this study was the identification of genes and pathways involved in the pathology of RAO by global transcriptome analyses in stimulated peripheral blood mononuclear cells (PBMCs). We performed RNA-seq on PBMCs derived from 40 RAO affected and 45 control horses belonging to three cohorts of Warmblood horses: two half-sib families and one group of unrelated horses. PBMCs were stimulated with hay dust extract, lipopolysaccharides, a recombinant parasite antigen, or left unstimulated. The total dataset consisted of 561 individual samples. We detected significant differences in the expression profiles between RAO and control horses. Differential expression (DE) was most marked upon stimulation with hay dust extract. An important novel finding was a strong upregulation of CXCL13 together with many genes involved in cell cycle regulation in stimulated samples from RAO affected horses, in addition to changes in the expression of several HIF-1 transcription factor target genes. The RAO condition alters systemic changes observed as differential expression profiles of PBMCs. Those changes also depended on the cohort and stimulation of the samples and were dominated by genes involved in immune cell trafficking, development, and cell cycle regulation. Our findings indicate an important role of CXCL13, likely macrophage or Th17 derived, and the cell cycle regulator CDC20 in the immune response in RAO.", "link"=>"http://www.mendeley.com/research/impaired-cell-cycle-regulation-natural-equine-model-asthma-1", "reader_count"=>16, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>3, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>3, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Biochemistry, Genetics and Molecular Biology"=>5, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>3, "Veterinary Science and Veterinary Medicine"=>1, "Computer Science"=>2, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84942941574"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84942941574?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84942941574&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84942941574&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84942941574", "dc:identifier"=>"SCOPUS_ID:84942941574", "eid"=>"2-s2.0-84942941574", "dc:title"=>"Impaired cell cycle regulation in a natural equine model of asthma", "dc:creator"=>"Pacholewska A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-08-20", "prism:coverDisplayDate"=>"20 August 2015", "prism:doi"=>"10.1371/journal.pone.0136103", "citedby-count"=>"11", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Bern", "affiliation-city"=>"Bern", "affiliation-country"=>"Switzerland"}], "pubmed-id"=>"26292153", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0136103", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0136103", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"52", "xml_views"=>"18", "html_views"=>"510"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"91", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"57", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"118", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"75", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"9", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"6", "html_views"=>"76"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"154"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2218540"], "description"=>"<p>The thermometers indicate the object regulating genes were up- (red) or downregulated (blue) by the RAO condition upon stimulation with 1) lipopolysaccharides (LPS), 2) recombinant cyathostomin antigen (RCA), and 3) hay dust extract (HDE). Lines indicate activation (green), inhibition (red) or unspecified (grey) interactions between the molecules. The object shapes correspond to molecule type and are described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136103#pone.0136103.s003\" target=\"_blank\">S3 Fig</a> and at <a href=\"https://portal.genego.com/legends/MetaCoreQuickReferenceGuide.pdf\" target=\"_blank\">https://portal.genego.com/legends/MetaCoreQuickReferenceGuide.pdf</a>.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515265, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g003", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GeneGo_pathway_8220_role_of_anaphase_promoting_complex_APC_in_cell_cycle_regulation_8221_/1515265", "title"=>"GeneGo pathway “role of anaphase-promoting complex (APC) in cell cycle regulation”.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218541"], "description"=>"<p>The bars represent the number of RAO-regulated genes by the overall RAO-effect, regardless stimulation and/or cohort.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515266, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g004", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Biological_processes_enriched_by_RAO_regulated_genes_/1515266", "title"=>"Biological processes enriched by RAO-regulated genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218543"], "description"=>"<p>The graphs represents the expression of <i>IL4R</i>, <i>IL4</i>, and <i>IL10</i> that were identified to have significantly increased expression levels in RAO horses in Family 1 by qRT-PCR [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136103#pone.0136103.ref018\" target=\"_blank\">18</a>]. The x-axes represent the stimulations: no stimulating factor (mock), lipopolysaccharides (LPS), recombinant cyathostomin antigen (RCA), and hay dust extract (HDE). On the logarithmic scaled (log 2) y-axes mean expression values are given in normalized counts per million. Error bars represent 95% confidence intervals. The stars (*) represent significant changes between expression levels of RAO (red dots and lines) and control (green dots and lines) samples at false discovery rate < 0.05.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515268, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g005", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_level_of_cytokines_previously_identified_as_RAO_upregulated_/1515268", "title"=>"Expression level of cytokines previously identified as RAO-upregulated.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218548"], "description"=>"<p>Expression levels of <i>IFNG</i>, <i>CXCL13</i>, <i>CDC20</i>, and <i>NDUFA4L2</i> are shown. The x-axes represent the stimulations: no stimulating factor (mock), lipopolysaccharides (LPS), recombinant cyathostomin antigen (RCA), and hay dust extract (HDE). On the logarithmic scaled (log 2) y-axes mean expression values are given in normalized counts per million. Error bars represent 95% confidence intervals. The stars (*) represent significant changes between expression levels of RAO (red dots and lines) and control (green dots and lines) samples at false discovery rate < 0.05.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515273, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g006", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_RAO_relevant_genes_/1515273", "title"=>"Expression of RAO-relevant genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218550"], "description"=>"<p>For every effect the number of DE genes and the top ten DE genes are listed. The overall “conditionRAO” effect includes significant genes due to the RAO effect in at least one cohort and stimulation. Mock-stimulated samples from control horses of the Unrelated cohort were used as a reference group. Baseline “conditionRAO” effect represents differences between RAO and control samples from the Unrelated unstimulated group. Colons “:” indicate an interaction effect, e.g. “conditionRAO:Family1” effect represents differential changes in the RAO effect in Family 1 in mock compared to RAO effect in Unrelated group in mock.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515275, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.t002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genes_regulated_by_RAO_status_with_further_dependencies_on_stimulating_agent_and_or_cohort_/1515275", "title"=>"Genes regulated by RAO-status with further dependencies on stimulating agent and/or cohort.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218551", "https://ndownloader.figshare.com/files/2218552", "https://ndownloader.figshare.com/files/2218553", "https://ndownloader.figshare.com/files/2218554", "https://ndownloader.figshare.com/files/2218555", "https://ndownloader.figshare.com/files/2218556", "https://ndownloader.figshare.com/files/2218557"], "description"=>"<div><p>Recurrent airway obstruction (RAO) is a common and potentially debilitating lower airway disease in horses, which shares many similarities with human asthma. In susceptible horses RAO exacerbation is caused by environmental allergens and irritants present in hay dust. The objective of this study was the identification of genes and pathways involved in the pathology of RAO by global transcriptome analyses in stimulated peripheral blood mononuclear cells (PBMCs). We performed RNA-seq on PBMCs derived from 40 RAO affected and 45 control horses belonging to three cohorts of Warmblood horses: two half-sib families and one group of unrelated horses. PBMCs were stimulated with hay dust extract, lipopolysaccharides, a recombinant parasite antigen, or left unstimulated. The total dataset consisted of 561 individual samples. We detected significant differences in the expression profiles between RAO and control horses. Differential expression (DE) was most marked upon stimulation with hay dust extract. An important novel finding was a strong upregulation of <i>CXCL13</i> together with many genes involved in cell cycle regulation in stimulated samples from RAO affected horses, in addition to changes in the expression of several HIF-1 transcription factor target genes. The RAO condition alters systemic changes observed as differential expression profiles of PBMCs. Those changes also depended on the cohort and stimulation of the samples and were dominated by genes involved in immune cell trafficking, development, and cell cycle regulation. Our findings indicate an important role of CXCL13, likely macrophage or Th17 derived, and the cell cycle regulator CDC20 in the immune response in RAO.</p></div>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515276, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0136103.s001", "https://dx.doi.org/10.1371/journal.pone.0136103.s002", "https://dx.doi.org/10.1371/journal.pone.0136103.s003", "https://dx.doi.org/10.1371/journal.pone.0136103.s004", "https://dx.doi.org/10.1371/journal.pone.0136103.s005", "https://dx.doi.org/10.1371/journal.pone.0136103.s006", "https://dx.doi.org/10.1371/journal.pone.0136103.s007"], "stats"=>{"downloads"=>17, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impaired_Cell_Cycle_Regulation_in_a_Natural_Equine_Model_of_Asthma_/1515276", "title"=>"Impaired Cell Cycle Regulation in a Natural Equine Model of Asthma", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218537"], "description"=>"<p>The figure shows a principal component analysis of the individual samples plotted across the three most variable components. The circles on the MDS plot represent the individual samples and are colored according to the 4 different stimulations: no stimulating factor (mock), lipopolysaccharides (LPS), recombinant cyathostomin antigen (RCA, both concentrations), and hay dust extract (HDE, all three concentrations).</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515262, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quality_control_of_the_RNA_seq_data_/1515262", "title"=>"Quality control of the RNA-seq data.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/2218538"], "description"=>"<p>Panel A illustrates the number of DE genes upon each stimulation across all horses. The panels B-E further differentiate this dependent on the three different cohorts. (B) Unstimulated cells (mock), followed by cells stimulated with (C) recombinant cyathostomin antigen (RCA), (D) lipopolysaccharides (LPS), and (E) hay dust extract (HDE). The numbers represent the number of significantly up (↗) and down (↘) regulated genes in RAO samples compared to control samples.</p>", "links"=>[], "tags"=>["Asthma Recurrent airway obstruction", "cell cycle regulator CDC 20", "transcription factor target genes", "cell cycle regulation", "horses RAO exacerbation", "expression profiles", "45 control horses", "pbmc", "cxcl", "hay dust", "Natural Equine Model", "de", "Impaired Cell Cycle Regulation", "sample", "hif"], "article_id"=>1515263, "categories"=>["Biological Sciences"], "users"=>["Alicja Pacholewska", "Vidhya Jagannathan", "Michaela Drögemüller", "Jolanta Klukowska-Rötzler", "Simone Lanz", "Eman Hamza", "Emmanouil T. Dermitzakis", "Eliane Marti", "Tosso Leeb", "Vincent Gerber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136103.g002", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Venn_diagrams_illustrating_the_number_of_DE_genes_between_RAO_and_control_horses_/1515263", "title"=>"Venn diagrams illustrating the number of DE genes between RAO and control horses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-20 03:07:25"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"34", "full-text"=>"27", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"33", "full-text"=>"31", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"11", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"41", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts