Cell-free DNA in Human Follicular Microenvironment: New Prognostic Biomarker to Predict in vitro Fertilization Outcomes
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{"title"=>"Cell-free DNA in human follicular microenvironment: New prognostic biomarker to predict in vitro fertilization outcomes", "type"=>"journal", "authors"=>[{"first_name"=>"Sabine", "last_name"=>"Traver", "scopus_author_id"=>"6508177196"}, {"first_name"=>"Elodie", "last_name"=>"Scalici", "scopus_author_id"=>"56562951500"}, {"first_name"=>"Tiffany", "last_name"=>"Mullet", "scopus_author_id"=>"56326699800"}, {"first_name"=>"Nicolas", "last_name"=>"Molinari", "scopus_author_id"=>"6701451724"}, {"first_name"=>"Claire", "last_name"=>"Vincens", "scopus_author_id"=>"6602795925"}, {"first_name"=>"Tal", "last_name"=>"Anahory", "scopus_author_id"=>"6602899688"}, {"first_name"=>"Samir", "last_name"=>"Hamamah", "scopus_author_id"=>"56276227600"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84942594654", "scopus"=>"2-s2.0-84942594654", "pui"=>"606153893", "pmid"=>"26288130", "doi"=>"10.1371/journal.pone.0136172", "issn"=>"19326203"}, "id"=>"399dd39a-22c2-3898-872e-16c47c8d1d6e", "abstract"=>"Cell-free DNA (cfDNA) fragments, detected in blood and in other biological fluids, are released from apoptotic and/or necrotic cells. CfDNA is currently used as biomarker for the detection of many diseases such as some cancers and gynecological and obstetrics disorders. In this study, we investigated if cfDNA levels in follicular fluid (FF) samples from in vitro fertilization (IVF) patients, could be related to their ovarian reserve status, controlled ovarian stimulation (COS) protocols and IVF outcomes. Therefore, 117 FF samples were collected from women (n = 117) undergoing IVF/Intra-cytoplasmic sperm injection (ICSI) procedure and cfDNA concentration was quantified by ALU-quantitative PCR. We found that cfDNA level was significantly higher in FF samples from patients with ovarian reserve disorders (low functional ovarian reserve or polycystic ovary syndrome) than from patients with normal ovarian reserve (2.7 +/- 2.7 ng/mul versus 1.7 +/- 2.3 ng/mul, respectively, p = 0.03). Likewise, FF cfDNA levels were significant more elevated in women who received long ovarian stimulation (> 10 days) or high total dose of gonadotropins (>/= 3000 IU/l) than in women who received short stimulation duration (7-10 days) or total dose of gonadotropins < 3000 IU/l (2.4 +/- 2.8 ng/mul versus 1.5 +/- 1.9 ng/mul, p = 0.008; 2.2 +/- 2.3 ng/mul versus 1.5 +/- 2.1 ng/mul, p = 0.01, respectively). Finally, FF cfDNA level was an independent and significant predictive factor for pregnancy outcome (adjusted odds ratio = 0.69 [0.5; 0.96], p = 0.03). In multivariate analysis, the Receiving Operator Curve (ROC) analysis showed that the performance of FF cfDNA in predicting clinical pregnancy reached 0.73 [0.66-0.87] with 88% specificity and 60% sensitivity. CfDNA might constitute a promising biomarker of follicular micro-environment quality which could be used to predict IVF prognosis and to enhance female infertility management.", "link"=>"http://www.mendeley.com/research/cellfree-dna-human-follicular-microenvironment-new-prognostic-biomarker-predict-vitro-fertilization", "reader_count"=>8, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>6, "Agricultural and Biological Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "reader_count_by_country"=>{"Ireland"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2216852"], "description"=>"<p><b>A</b>, Follicular fluid cfDNA content in patients with normal ovarian reserve versus patients with ovarian reserve disorders (ovarian insufficiency and polycystic ovary syndrome); *p = 0.03. <b>B</b>, Follicular fluid cfDNA content according to the ovarian reserve parameters; left panel: AFC (<10 versus ≥ 10, *p = 0.04); right panel: AMH (≤ 1 versus > 1 ng/ml, *p = 0.06). <b>C</b>, Follicular fluid cfDNA levels according to the infertility length (1 versus ≥ 5 years, *p = 0.049).</p>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514257, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136172.g001", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cell_free_DNA_level_in_follicular_fluid_pools_according_to_the_patients_8217_ovarian_reserve_status_ovarian_reserve_parameters_and_infertility_length_/1514257", "title"=>"Cell-free DNA level in follicular fluid pools according to the patients’ ovarian reserve status, ovarian reserve parameters and infertility length.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-19 03:13:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2216854"], "description"=>"<p><b>A</b>, Follicular fluid cfDNA content according to the length of ovarian stimulation (≤ 10 versus > 10 days, *p = 0.008). <b>B</b>, Follicular fluid cfDNA content according to the total dose of gonadotropins (<3000 versus ≥3000 IU/l, *p = 0.01). <b>C</b>, Follicular fluid cfDNA content according to the number of retrieved oocytes (≤ 6 versus > 6 oocytes, *p = 0.045).</p>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514259, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136172.g002", "stats"=>{"downloads"=>4, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CfDNA_level_in_follicular_fluid_pools_according_to_the_ovarian_stimulation_protocol_and_ovarian_response_/1514259", "title"=>"CfDNA level in follicular fluid pools according to the ovarian stimulation protocol and ovarian response.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-19 03:13:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2216855"], "description"=>"<p><b>A</b>, Follicular Fluid cfDNA content according to the total number of embryos at day 2 (≤ 2 versus > 2, *p = 0.03). <b>B</b>, Follicular Fluid cfDNA content according to, left panel: the number of top quality (grade 1–2) embryos per patient (0 versus ≥ 1, *p = 0.002) at day 2, right panel: ratio between number of top quality embryos and total number of embryos (< 0.2 versus ≥ 0.2, *p = 0.04) at day 2. <b>C</b>, Follicular Fluid cfDNA content according to, left panel: number of top quality (grade 1–2) embryos per patient (0 versus ≥ 1, *p = 0.006) at day 3, right panel: ratio between number of top quality embryo and total number of embryos (< 0.2 versus ≥ 0.2, *p = 0.02) at day 3. <b>D</b>, Follicular Fluid cfDNA content according to, left panel: fragmentation rate at day 3 (<20% versus ≥ 20%, p = 0.18) and right panel: ratio between blastomere number and total embryo number at day 3 (<6 versus 6–8, *p = 0.02).</p>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514260, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136172.g003", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CfDNA_level_in_follicular_fluid_pools_according_to_the_embryo_outcome_at_day_2_and_3_/1514260", "title"=>"CfDNA level in follicular fluid pools according to the embryo outcome at day 2 and 3.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-19 03:13:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2216856"], "description"=>"<p>ROC curve to evaluate the predictive value of follicular fluid cfDNA level for clinical pregnancy outcome in a multivariate model (including the rank of IVF/ICSI attempts and the number of embryos): area under the curve = 0.73 [0.66–0.87], sensitivity = 60%, specificity = 88%.</p>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514261, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136172.g004", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ROC_curve_to_evaluate_the_predictive_value_of_follicular_fluid_cfDNA_level_for_clinical_pregnancy_outcome_in_a_multivariate_model_including_the_rank_of_IVF_ICSI_attempts_and_the_number_of_embryos_area_under_the_curve_0_73_0_66_8211_0_87_sensitivity_60_sp/1514261", "title"=>"ROC curve to evaluate the predictive value of follicular fluid cfDNA level for clinical pregnancy outcome in a multivariate model (including the rank of IVF/ICSI attempts and the number of embryos): area under the curve = 0.73 [0.66–0.87], sensitivity = 60%, specificity = 88%.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-19 03:13:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2216858"], "description"=>"<p>High cell-free DNA levels in human FF reflect a poor quality of follicular micro-environment and consequently are related to a poor IVF prognosis. r-FSH, recombinant FSH.</p>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514263, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136172.g005", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_model_summarizing_the_significant_relationships_between_cell_free_DNA_levels_in_human_follicular_fluid_FF_and_infertility_length_ovarian_reserve_status_ovarian_stimulation_ovarian_response_to_stimulation_embryo_development_and_clinical_pregnanc/1514263", "title"=>"Schematic model summarizing the significant relationships between cell-free DNA levels in human follicular fluid (FF) and: infertility length, ovarian reserve status, ovarian stimulation, ovarian response to stimulation, embryo development and clinical pregnancy outcomes, respectively.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-08-19 03:13:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/2216859", "https://ndownloader.figshare.com/files/2216860", "https://ndownloader.figshare.com/files/2216861", "https://ndownloader.figshare.com/files/2216862"], "description"=>"<div><p>Cell-free DNA (cfDNA) fragments, detected in blood and in other biological fluids, are released from apoptotic and/or necrotic cells. CfDNA is currently used as biomarker for the detection of many diseases such as some cancers and gynecological and obstetrics disorders. In this study, we investigated if cfDNA levels in follicular fluid (FF) samples from <i>in vitro</i> fertilization (IVF) patients, could be related to their ovarian reserve status, controlled ovarian stimulation (COS) protocols and IVF outcomes. Therefore, 117 FF samples were collected from women (n = 117) undergoing IVF/Intra-cytoplasmic sperm injection (ICSI) procedure and cfDNA concentration was quantified by ALU-quantitative PCR. We found that cfDNA level was significantly higher in FF samples from patients with ovarian reserve disorders (low functional ovarian reserve or polycystic ovary syndrome) than from patients with normal ovarian reserve (2.7 ± 2.7 ng/μl versus 1.7 ± 2.3 ng/μl, respectively, p = 0.03). Likewise, FF cfDNA levels were significant more elevated in women who received long ovarian stimulation (> 10 days) or high total dose of gonadotropins (≥ 3000 IU/l) than in women who received short stimulation duration (7–10 days) or total dose of gonadotropins < 3000 IU/l (2.4 ± 2.8 ng/μl versus 1.5 ± 1.9 ng/μl, p = 0.008; 2.2 ± 2.3 ng/μl versus 1.5 ± 2.1 ng/μl, p = 0.01, respectively). Finally, FF cfDNA level was an independent and significant predictive factor for pregnancy outcome (adjusted odds ratio = 0.69 [0.5; 0.96], p = 0.03). In multivariate analysis, the Receiving Operator Curve (ROC) analysis showed that the performance of FF cfDNA in predicting clinical pregnancy reached 0.73 [0.66–0.87] with 88% specificity and 60% sensitivity. CfDNA might constitute a promising biomarker of follicular micro-environment quality which could be used to predict IVF prognosis and to enhance female infertility management.</p></div>", "links"=>[], "tags"=>["icsi", "cfDNA concentration", "reserve disorders", "IVF outcomes", "pcr", "Human Follicular Microenvironment", "FF samples", "roc", "cfDNA levels", "117 FF samples", "reserve status", "cos", "Fertilization Outcomes", "Multivariate analysis", "odds ratio", "stimulation", "infertility management", "Polycystic Ovary Syndrome", "Operator Curve", "cfDNA level", "FF cfDNA", "FF cfDNA levels", "pregnancy outcome", "New Prognostic Biomarker", "ng", "IVF prognosis", "obstetrics disorders", "iu", "FF cfDNA level"], "article_id"=>1514264, "categories"=>["Biological Sciences"], "users"=>["Sabine Traver", "Elodie Scalici", "Tiffany Mullet", "Nicolas Molinari", "Claire Vincens", "Tal Anahory", "Samir Hamamah"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0136172.s001", "https://dx.doi.org/10.1371/journal.pone.0136172.s002", "https://dx.doi.org/10.1371/journal.pone.0136172.s003", "https://dx.doi.org/10.1371/journal.pone.0136172.s004"], "stats"=>{"downloads"=>22, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Cell_free_DNA_in_Human_Follicular_Microenvironment_New_Prognostic_Biomarker_to_Predict_in_vitro_Fertilization_Outcomes/1514264", "title"=>"Cell-free DNA in Human Follicular Microenvironment: New Prognostic Biomarker to Predict <i>in vitro</i> Fertilization Outcomes", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-08-19 03:13:58"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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