Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications
Publication Date
September 01, 2015
Journal
PLOS ONE
Authors
Antonios A. Augustinos, Georgios A. Kyritsis, Nikos T. Papadopoulos, Adly M. M. Abd Alla, et al
Volume
10
Issue
9
Pages
e0136459
DOI
https://dx.plos.org/10.1371/journal.pone.0136459
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0136459
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26325068
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556606
Europe PMC
http://europepmc.org/abstract/MED/26325068
Web of Science
000360437700044
Scopus
84943328600
Mendeley
http://www.mendeley.com/research/exploitation-medfly-gut-microbiota-enhancement-sterile-insect-technique-enterobacter-sp-larval-dietb
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/2248877"], "description"=>"<p>i) irrespective of the <i>Enterobacter</i> sp. concentration, or ii) considering the three different <i>Enterobacter</i> sp. concentrations as different treatments. Columns marked on top with the same letter are not significantly different (P>0.05)</p>", "links"=>[], "tags"=>["gss", "sit", "probiotic", "fitness", "Medfly Gut Microbiota", "Enterobacter sp", "Vienna 8 strain", "facility", "Sterile Insect Technique", "species", "application", "pupal"], "article_id"=>1532254, "categories"=>["Uncategorised"], "users"=>["Antonios A. Augustinos", "Georgios A. Kyritsis", "Nikos T. Papadopoulos", "Adly M. M. Abd-Alla", "Carlos Cáceres", "Kostas Bourtzis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136459.g002", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pupae_A_and_adult_B_recovery_rates_/1532254", "title"=>"Pupae (A) and adult (B) recovery rates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-01 03:08:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/2248885", "https://ndownloader.figshare.com/files/2248886", "https://ndownloader.figshare.com/files/2248887", "https://ndownloader.figshare.com/files/2248888", "https://ndownloader.figshare.com/files/2248889", "https://ndownloader.figshare.com/files/2248890", "https://ndownloader.figshare.com/files/2248891", "https://ndownloader.figshare.com/files/2248892"], "description"=>"<div><p>The Mediterranean fruit fly (medfly), <i>Ceratitis capitata</i>, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS) for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, <i>Enterobacter</i> sp., as dietary supplement (probiotic) to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of <i>Enterobacter</i> sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.</p></div>", "links"=>[], "tags"=>["gss", "sit", "probiotic", "fitness", "Medfly Gut Microbiota", "Enterobacter sp", "Vienna 8 strain", "facility", "Sterile Insect Technique", "species", "application", "pupal"], "article_id"=>1532262, "categories"=>["Uncategorised"], "users"=>["Antonios A. Augustinos", "Georgios A. Kyritsis", "Nikos T. Papadopoulos", "Adly M. M. Abd-Alla", "Carlos Cáceres", "Kostas Bourtzis"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0136459.s001", "https://dx.doi.org/10.1371/journal.pone.0136459.s002", "https://dx.doi.org/10.1371/journal.pone.0136459.s003", "https://dx.doi.org/10.1371/journal.pone.0136459.s004", "https://dx.doi.org/10.1371/journal.pone.0136459.s005", "https://dx.doi.org/10.1371/journal.pone.0136459.s006", "https://dx.doi.org/10.1371/journal.pone.0136459.s007", "https://dx.doi.org/10.1371/journal.pone.0136459.s008"], "stats"=>{"downloads"=>11, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Exploitation_of_the_Medfly_Gut_Microbiota_for_the_Enhancement_of_Sterile_Insect_Technique_Use_of_Enterobacter_sp_in_Larval_Diet_Based_Probiotic_Applications/1532262", "title"=>"Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of <i>Enterobacter</i> sp. in Larval Diet-Based Probiotic Applications", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-01 03:08:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/2248880"], "description"=>"<p>The mating competitiveness tests were performed in accordance to [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136459#pone.0136459.ref038\" target=\"_blank\">38</a>].</p>", "links"=>[], "tags"=>["gss", "sit", "probiotic", "fitness", "Medfly Gut Microbiota", "Enterobacter sp", "Vienna 8 strain", "facility", "Sterile Insect Technique", "species", "application", "pupal"], "article_id"=>1532257, "categories"=>["Uncategorised"], "users"=>["Antonios A. Augustinos", "Georgios A. Kyritsis", "Nikos T. Papadopoulos", "Adly M. M. Abd-Alla", "Carlos Cáceres", "Kostas Bourtzis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136459.g004", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mating_competitiveness_of_Enterobacter_sp_treated_males_Relative_Index_/1532257", "title"=>"Mating competitiveness of <i>Enterobacter</i> sp. treated males (Relative Index).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-01 03:08:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/2248878"], "description"=>"<p>(A) egg to pupa duration, (B) pupal stage duration, (C) total duration of immature stages. The top and bottom of the box are the 25th and 75th percentiles (Q(0.25) and Q(0.75), respectively). The size of the box (Interquartile Range-IQR) is defined as IQR = Q(0.75)<b>-</b>Q(0.25). The bold line in the box represents the median.</p>", "links"=>[], "tags"=>["gss", "sit", "probiotic", "fitness", "Medfly Gut Microbiota", "Enterobacter sp", "Vienna 8 strain", "facility", "Sterile Insect Technique", "species", "application", "pupal"], "article_id"=>1532255, "categories"=>["Uncategorised"], "users"=>["Antonios A. Augustinos", "Georgios A. Kyritsis", "Nikos T. Papadopoulos", "Adly M. M. Abd-Alla", "Carlos Cáceres", "Kostas Bourtzis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136459.g003", "stats"=>{"downloads"=>19, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Developmental_times_of_immature_stages_at_22_176_C_/1532255", "title"=>"Developmental times of immature stages at 22°C.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-01 03:08:04"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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