Gliadin Induces Neutrophil Migration via Engagement of the Formyl Peptide Receptor, FPR1
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{"title"=>"Gliadin induces neutrophil migration via engagement of the formyl peptide receptor, FPR1", "type"=>"journal", "authors"=>[{"first_name"=>"Karen M.", "last_name"=>"Lammers", "scopus_author_id"=>"6603022383"}, {"first_name"=>"Marcello", "last_name"=>"Chieppa", "scopus_author_id"=>"9639838400"}, {"first_name"=>"Lunhua", "last_name"=>"Liu", "scopus_author_id"=>"56093127100"}, {"first_name"=>"Song", "last_name"=>"Liu", "scopus_author_id"=>"56937184900"}, {"first_name"=>"Tatsushi", "last_name"=>"Omatsu", "scopus_author_id"=>"56938177100"}, {"first_name"=>"Mirkka", "last_name"=>"Janka-Junttila", "scopus_author_id"=>"14422363400"}, {"first_name"=>"Vincenzo", "last_name"=>"Casolaro", "scopus_author_id"=>"7003526446"}, {"first_name"=>"Hans Christian", "last_name"=>"Reinecker", "scopus_author_id"=>"7004088746"}, {"first_name"=>"Carole A.", "last_name"=>"Parent", "scopus_author_id"=>"7006118108"}, {"first_name"=>"Alessio", "last_name"=>"Fasano", "scopus_author_id"=>"7101744887"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"606624534", "pmid"=>"26378785", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0138338", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-84945929451", "sgr"=>"84945929451"}, "id"=>"8afcb69e-1a4c-38ce-a00c-2a103a6dd42b", "abstract"=>"BACKGROUND: Gliadin, the immunogenic component within gluten and trigger of celiac disease, is known to induce the production of Interleukin-8, a potent neutrophil-activating and chemoattractant chemokine. We sought to study the involvement of neutrophils in the early immunological changes following gliadin exposure.\\n\\nMETHODS: Utilizing immunofluorescence microscopy and flow cytometry, the redistribution of major tight junction protein, Zonula occludens (ZO)-1, and neutrophil recruitment were assessed in duodenal tissues of gliadin-gavaged C57BL/6 wild-type and Lys-GFP reporter mice, respectively. Intravital microscopy with Lys-GFP mice allowed monitoring of neutrophil recruitment in response to luminal gliadin exposure in real time. In vitro chemotaxis assays were used to study murine and human neutrophil chemotaxis to gliadin, synthetic alpha-gliadin peptides and the neutrophil chemoattractant, fMet-Leu-Phe, in the presence or absence of a specific inhibitor of the fMet-Leu-Phe receptor-1 (FPR1), cyclosporine H. An irrelevant protein, zein, served as a control.\\n\\nRESULTS: Redistribution of ZO-1 and an influx of CD11b+Lys6G+ cells in the lamina propria of the small intestine were observed upon oral gavage of gliadin. In vivo intravital microscopy revealed a slowing down of GFP+ cells within the vessels and influx in the mucosal tissue within 2 hours after challenge. In vitro chemotaxis assays showed that gliadin strongly induced neutrophil migration, similar to fMet-Leu-Phe. We identified thirteen synthetic gliadin peptide motifs that induced cell migration. Blocking of FPR1 completely abrogated the fMet-Leu-Phe-, gliadin- and synthetic peptide-induced migration.\\n\\nCONCLUSIONS: Gliadin possesses neutrophil chemoattractant properties similar to the classical neutrophil chemoattractant, fMet-Leu-Phe, and likewise uses FPR1 in the process.", "link"=>"http://www.mendeley.com/research/gliadin-induces-neutrophil-migration-via-engagement-formyl-peptide-receptor-fpr1", "reader_count"=>33, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>5, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>5, "Student > Master"=>4, "Other"=>3, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>5, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>5, "Student > Master"=>4, "Other"=>3, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>7, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Computer Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>5}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2269540"], "description"=>"<p>Four alpha-gliadin synthetic peptides that displayed a chemotactic response were elected and tested in the presence of cyclosporine H, a specific inhibitor of FPR1. Blocking of FPR1 inhibited neutrophil chemotaxis to these peptides.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547550, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.t003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blocking_of_FPR1_inhibits_neutrophil_migration_to_gliadin_peptides_/1547550", "title"=>"Blocking of FPR1 inhibits neutrophil migration to gliadin peptides.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269537"], "description"=>"<p>Flow cytometry analysis of FITC-labeled gliadin peptide or fMet-Leu-Phe binding to FPR1-transfected or non-transfected HEK293T cells. (A) Kinetic binding of FITC-labeled gliadin synthetic peptide, TLPAMCNVYIPPYCTIVPFG, applied at increasing concentrations (ranging from 1 to 250 μM). Dissociation constant (K<sub>d</sub>) was 235.1 μM, and Bmax was 922.4. (B) Kinetic binding of FITC-labeled fMet-Leu-Phe, applied at increasing concentrations (ranging from 1 to 1000 nM). Dissociation constant (K<sub>d</sub>) was 27.6 nM, and Bmax was 261.6. (C) Competitive binding assay was performed with FITC-labeled gliadin peptide at 25.6 μM and unlabeled fMet-Leu-Phe at increasing concentrations (ranging from 10 nM to 2 mM). fMet-Leu-Phe caused a displacement of the gliadin peptide from the FPR1-transfected HEK293T cells with an IC50 of 2.04 μM. (D) Competitive binding assay was performed with FITC-labeled fMet-Leu-Phe at 100 nM and unlabeled gliadin peptide at increasing concentrations (ranging from 1 nM to 500 μM). The gliadin peptide was not capable of displacement of the fMet-Leu-Phe from the FPR1-transfected HEK293T cells. Binding to non-transfected cells and FPR1-transfected HEK293T cells is depicted with white and black circles, respectively. Each graph represents data from 3–5 independent experiments.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547547, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.g005", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_fMet_Leu_Phe_and_PT_gliadin_to_FPR1_/1547547", "title"=>"Binding of fMet-Leu-Phe and PT-gliadin to FPR1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269536"], "description"=>"<p>(A-C) Data were obtained using the EZ-TAXIScan assay. (A) Images from representative EZ-TAXIScan movies (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138338#pone.0138338.s004\" target=\"_blank\">S3 movie</a>). (B) Image shows the paths of individual cell migration to PT-water, PT-zein, PT-gliadin or fMet-Leu-Phe. The colors progress from red to blue as a function of time. (C) Quantitative analysis of all EZ-TAXIScan assay results of human neutrophil chemotaxis in response to PT-water, PT-zein, PT-gliadin and fMet-Leu-Phe. The table depicts speed (μm/min) and the chemotactic index (CI). PT-gliadin induced neutrophil chemotaxis with speed and CI similar to fMet-Leu-Phe (<i>P</i> = NS). Data are representative of results obtained from four independent movies. (D) PT-gliadin induced neutrophil migration in a dose-dependent manner in a Transwell chemotaxis assay (<i>P</i><0.01). Data are obtained from six independent experiments. (E) PT-gliadin induced neutrophil migration in a dose-dependent manner in the EZ-TAXIScan assay. As a positive control, fMet-Leu-Phe was used. Data are obtained from three independent experiments.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547546, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.g004", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Human_neutrophils_migrate_to_fMet_Leu_Phe_and_PT_gliadin_/1547546", "title"=>"Human neutrophils migrate to fMet-Leu-Phe and PT-gliadin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269530"], "description"=>"<p>(A) Image stills at different time points from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138338#pone.0138338.s002\" target=\"_blank\">S1 movie</a>. Intravital microscopy of the duodenum of Lys-GFP mice showed a rapid extravasation and recruitment of neutrophils (bright green) within 1 hour after PT-gliadin luminal administration, which was absent in mice treated with the same volume of PBS. Data are representative of results obtained from eight independent experiments (<i>n</i> = 8 for PT-gliadin, <i>n</i> = 8 for PBS). (B) Quantitative analysis of neutrophil recruitment in response to intestinal luminal PT-gliadin challenge. The green fluorescent channel in the original images was extracted, converted and processed into 16 bit binary images using the Image J software. The number of bright spots, which represents the number of neutrophils in the movies, was calculated with Image J using the Analyze Particles function. Neutrophil recruitment started immediately upon intestinal gliadin exposure, became significant after 15 minutes (<i>P</i> = .006) and increased further over time (<i>P</i> = .019 at <i>t</i> = 30 minutes).</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547540, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.g001", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vivo_intestinal_luminal_injection_of_PT_gliadin_induces_an_immediate_and_considerable_neutrophil_migration_/1547540", "title"=>"<i>In-vivo</i> intestinal luminal injection of PT-gliadin induces an immediate and considerable neutrophil migration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269539"], "description"=>"<p>Alpha-gliadin synthetic peptides were tested for their capacity to induce neutrophil chemotaxis. Thirteen peptides induced neutrophil chemotaxis.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547549, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.t002", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Synthetic_peptides_of_the_alpha_gliadin_peptide_library_induce_neutrophil_migration_/1547549", "title"=>"Synthetic peptides of the alpha-gliadin peptide library induce neutrophil migration.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269538"], "description"=>"<p>Pretreatment of neutrophils with cyclosporine H, a specific inhibitor of FPR1, completely abrogated the neutrophil migration induced by PT-gliadin and fMet-Leu-Phe. As expected, LTB<sub>4</sub> induced neutrophil chemotaxis was not affected by cyclosporine H.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547548, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.t001", "stats"=>{"downloads"=>2, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blocking_of_FPR1_abrogates_neutrophil_migration_to_fMet_Leu_Phe_and_gliadin_/1547548", "title"=>"Blocking of FPR1 abrogates neutrophil migration to fMet-Leu-Phe and gliadin.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269571", "https://ndownloader.figshare.com/files/2269572", "https://ndownloader.figshare.com/files/2269574", "https://ndownloader.figshare.com/files/2269577", "https://ndownloader.figshare.com/files/2269578", "https://ndownloader.figshare.com/files/2269579", "https://ndownloader.figshare.com/files/2269580"], "description"=>"<div><p>Background</p><p>Gliadin, the immunogenic component within gluten and trigger of celiac disease, is known to induce the production of Interleukin-8, a potent neutrophil-activating and chemoattractant chemokine. We sought to study the involvement of neutrophils in the early immunological changes following gliadin exposure.</p><p>Methods</p><p>Utilizing immunofluorescence microscopy and flow cytometry, the redistribution of major tight junction protein, Zonula occludens (ZO)-1, and neutrophil recruitment were assessed in duodenal tissues of gliadin-gavaged C57BL/6 wild-type and Lys-GFP reporter mice, respectively. Intravital microscopy with Lys-GFP mice allowed monitoring of neutrophil recruitment in response to luminal gliadin exposure in real time. <i>In vitro</i> chemotaxis assays were used to study murine and human neutrophil chemotaxis to gliadin, synthetic alpha-gliadin peptides and the neutrophil chemoattractant, fMet-Leu-Phe, in the presence or absence of a specific inhibitor of the fMet-Leu-Phe receptor-1 (FPR1), cyclosporine H. An irrelevant protein, zein, served as a control.</p><p>Results</p><p>Redistribution of ZO-1 and an influx of CD11b<sup>+</sup>Lys6G<sup>+</sup> cells in the <i>lamina propria</i> of the small intestine were observed upon oral gavage of gliadin. <i>In vivo</i> intravital microscopy revealed a slowing down of GFP<sup>+</sup> cells within the vessels and influx in the mucosal tissue within 2 hours after challenge. <i>In vitro</i> chemotaxis assays showed that gliadin strongly induced neutrophil migration, similar to fMet-Leu-Phe. We identified thirteen synthetic gliadin peptide motifs that induced cell migration. Blocking of FPR1 completely abrogated the fMet-Leu-Phe-, gliadin- and synthetic peptide-induced migration.</p><p>Conclusions</p><p>Gliadin possesses neutrophil chemoattractant properties similar to the classical neutrophil chemoattractant, fMet-Leu-Phe, and likewise uses FPR1 in the process.</p></div>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547562, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0138338.s001", "https://dx.doi.org/10.1371/journal.pone.0138338.s002", "https://dx.doi.org/10.1371/journal.pone.0138338.s003", "https://dx.doi.org/10.1371/journal.pone.0138338.s004", "https://dx.doi.org/10.1371/journal.pone.0138338.s005", "https://dx.doi.org/10.1371/journal.pone.0138338.s006", "https://dx.doi.org/10.1371/journal.pone.0138338.s007"], "stats"=>{"downloads"=>10, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gliadin_Induces_Neutrophil_Migration_via_Engagement_of_the_Formyl_Peptide_Receptor_FPR1_/1547562", "title"=>"Gliadin Induces Neutrophil Migration via Engagement of the Formyl Peptide Receptor, FPR1", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269534"], "description"=>"<p>(A) C57BL/6 intestinal segments did not show release of KC during the 2 hours incubation with PT-gliadin (<i>P</i> = NS). The graph represents data obtained from triplicate cultures from three mice. (B-D) EZ-TAXIScan chemotaxis of neutrophils isolated from the bone marrow of C57BL/6 mice toward medium alone, PT-gliadin or fMet-Leu-Phe (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138338#pone.0138338.s003\" target=\"_blank\">S2 movie</a>). (B) Images from representative EZ-TAXIScan movies. (C) Image shows the paths of individual cells migrating to PT-water, PT-zein, PT-gliadin (1 mg/mL) or fMet-Leu-Phe (100 nM). The colors progress from red to blue as a function of time. (D) Quantitative analysis of all EZ-TAXIScan assay results of murine neutrophil chemotaxis in response to medium alone, PT-gliadin and fMet-Leu-Phe. The table depicts speed (μm/min) and the chemotactic index (considering speed and distance). Data are representative of results obtained from six independent movies.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547543, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.g003", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PT_gliadin_attracts_neutrophils_but_does_not_induce_a_rapid_release_of_murine_KC_CXCL1_/1547543", "title"=>"PT-gliadin attracts neutrophils but does not induce a rapid release of murine KC/CXCL1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-17 03:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2269532"], "description"=>"<p>Representative images of C57BL/6 duodenum stained with antibodies specific for TJ protein, ZO-1 (green; A, B). Tissue sections were obtained from the duodenum of four mice 2 hours after gavage with PT-gliadin (1 mg/mL; B) or the same volume of drinking water (A) (<i>n</i> = 3). (C) Flow-cytometric analysis of CD11b<sup>+</sup>Ly6G<sup>+</sup> cells isolated from the <i>lamina propria</i> of PBS-, PT-gliadin or PT-zein-gavaged Lys-GFP mice (cells were pooled from 2 mice per group). Compared to control and PT-zein, PT-gliadin ingestion induced an increased tissue influx of CD11b<sup>+</sup>Ly6G<sup>+</sup> myeloid cells in the gut <i>lamina propria</i>. PBS <i>vs</i>. PT-gliadin, <i>P</i> = .0035; PT-zein <i>vs</i>. PT-gliadin, <i>P</i> = .0025. The graph represents data obtained from three separate experiments. (D) Representative dot plots of the flow cytometric analyses of the data depicted in graph 2C.</p>", "links"=>[], "tags"=>["gliadin exposure.MethodsUtilizing immunofluorescence microscopy", "neutrophil recruitment", "FPR 1", "CD 11b cells", "vivo intravital microscopy", "gliadin peptide motifs", "Formyl Peptide Receptor", "57BL", "FPR 1 BackgroundGliadin", "zo", "neutrophil chemoattractant properties", "Gliadin Induces Neutrophil Migration", "chemotaxis assays", "luminal gliadin exposure", "neutrophil chemoattractant", "gfp"], "article_id"=>1547542, "categories"=>["Uncategorised"], "users"=>["Karen M. Lammers", "Marcello Chieppa", "Lunhua Liu", "Song Liu", "Tatsushi Omatsu", "Mirkka Janka-Junttila", "Vincenzo Casolaro", "Hans-Christian Reinecker", "Carole A. Parent", "Alessio Fasano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138338.g002", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gliadin_causes_redistribution_of_tight_junction_proteins_ZO_1_and_recruits_CD11b_Ly6G_cells_into_the_lamina_propria_/1547542", "title"=>"Gliadin causes redistribution of tight junction proteins, ZO-1, and recruits CD11b<sup>+</sup>Ly6G<sup>+</sup> cells into the <i>lamina propria</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-17 03:40:56"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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