In Vivo Evolution of Bacterial Resistance in Two Cases of Enterobacter aerogenes Infections during Treatment with Imipenem
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Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/2285930"], "description"=>"<p>This figure was generated with GCviewer (<a href=\"http://stothard.afns.ualberta.ca/cgview_server/\" target=\"_blank\">http://stothard.afns.ualberta.ca/cgview_server/</a>) and shows the map of the chromosome and plasmid pGPN1 (174 Kbp) of <i>E</i>. <i>aerogenes</i> isolate G7 obtained by <i>de novo</i> sequencing and assembly.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554203, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.g004", "stats"=>{"downloads"=>2, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genome_of_E_aerogenes_G7_/1554203", "title"=>"Genome of <i>E</i>. <i>aerogenes</i> G7.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285929"], "description"=>"<p>Strains were grown in LB broth for 24 h at 37°C and cells were harvested by centrifugation. Cells were deenergized by an overnight incubation in Mg-PPB pH 7.0 with 5 μM CCCP and loaded with 1,2’-DNA. Cells were then washed, resuspended in Mg-PPB pH 7.0 and 1,2’-DNA efflux was monitored after rapid reenergization with glucose. RFU, relative fluorescence units. All values are means of at least three independent experiments.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554202, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Real_time_efflux_of_1_2_DNA_in_E_aerogenes_strains_/1554202", "title"=>"Real-time efflux of 1,2'-DNA in <i>E</i>. <i>aerogenes</i> strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285932"], "description"=>"<p><i>E</i>. <i>aerogenes</i> isolates were recovered periodically from two patients undergoing chemotherapy with imipenem—P1 to P5 from patient P and G1 to G7 from patient G. ATCC15038 was used as a reference strain. MICs were determined by broth microdilution in MHII broth as described in Materials and Methods. For clarity, strains have been classified as R for resistant and S for susceptible. Detailed MICs are presented in supplementary information (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138828#pone.0138828.s007\" target=\"_blank\">S1 Table</a>). Different classes of antibiotics were tested: phenicols (chloramphenicol), quinolones (nalidixic acid, fleroxacin and norfloxacin), macrolides (erythromycin), carbapenems (imipenem and ertapenem), cephalosporins (cefepime, ceftazidime and cefotaxime), tetracycline and polymyxin B. Gentamicin is often the last option to treat MDR strains. For this reasons, we chose not to include this antibiotic in our assays but we refer to MIC values obtained from the healthcare facilities.</p><p><sup><i>a</i></sup> Sampling sites: U, urine; V, vagina; S, Sputum; B, blood; T, trachea.</p><p><sup><i>b</i></sup> The presence of porins and efflux pump proteins were determined immunoblots using specific antibodies directed against <i>Escherichia coli</i> OmpF, OmpC, AcrA, AcrB or TolC.</p><p>*Mass spectrometry analysis indicated that porin-plus isolates only express Omp36 whereas strain ATCC15038 expresses both Omp36 and Omp35 (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138828#pone.0138828.s004\" target=\"_blank\">S1 Fig</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138828#pone.0138828.s008\" target=\"_blank\">S2 Table</a>).</p><p>Phenotypic characterization of <i>E</i>. <i>aerogenes</i> strains.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554205, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.t001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phenotypic_characterization_of_E_aerogenes_strains_/1554205", "title"=>"Phenotypic characterization of <i>E</i>. <i>aerogenes</i> strains.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285931"], "description"=>"<p>Plain arrows represent the putative evolution of bacterial subpopulations in patient P (A) and patient G (B). The genomic distance between the first isolates (P1 and G1) and the subsequent strains (P2-P5 and G2-G7) indicates that these have evolved from other ancestors (P0 and G0). Lack of evidence of genotypic reversion (Rev) from resistant to susceptible strains within the G series suggest alternative evolutionary pathways represented by dotted arrows. Clinical timelines are indicated from day (D) 1 to D12 and week (W) 1 to W13 for patient P and patient G, respectively. IMI, imipenem; CEF, cefpirome.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554204, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.g005", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_of_intra_patient_evolution_of_E_aerogenes_during_antibiotic_treatment_/1554204", "title"=>"Model of intra-patient evolution of <i>E</i>. <i>aerogenes</i> during antibiotic treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285926"], "description"=>"<p>Strains were grown in LB broth and cells were harvested at the beginning of the stationary phase. Cells were washed, resuspended in PPB pH 7.0, and the H33342 influx was assayed in the presence of 50 μM CCCP. A. Chemical structure of H33342. B. Kinetics of H33342 influx. AU, arbitrary units. C. Total accumulation in <i>E</i>. <i>aerogenes</i> isolates after normalization to that of strain ATCC15038. All values are means of at least three independent experiments.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554199, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.g001", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hoechst_33342_influx_into_E_aerogenes_strains_/1554199", "title"=>"Hoechst 33342 influx into <i>E</i>. <i>aerogenes</i> strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285927"], "description"=>"<p>Strains were grown in LB broth and cells were harvested at mid-log phase. Cells were washed and concentrated 10-fold in NaPi pH 7.0 supplemented with Mg<sup>2+</sup>. Fleroxacin (FLX) was added to the bacterial suspensions in the absence or in the presence of 10 μM CCCP. After a 30 min incubation time, bacterial lysates were prepared as described in Materials and Methods. The concentration of FLX in the lysates was estimated after normalization of the spectra to their corresponding tryptophan emission peak at 356 nm. The antibiotic-free control time-zero was subtracted from the values obtained for each assay. All values are means of at least three independent experiments.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554200, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fleroxacin_accumulation_in_E_aerogenes_strains_/1554200", "title"=>"Fleroxacin accumulation in <i>E</i>. <i>aerogenes</i> strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-23 03:54:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2285934"], "description"=>"<p>CDS, coding sequences; ICE, integrative and conjugative element; IS, insertion sequence. * Data refer to the chromosome only, except when plasmids are indicated.</p>", "links"=>[], "tags"=>["encoding sensor kinases", "membrane permeability", "nosocomial pathogen", "control membrane permeability", "Enterobacter aerogenes Infections", "Bacterial Resistance", "antimicrobial strategies", "transport assays", "antibiotic", "mdr", "omp 35", "Enterobacter aerogenes", "membrane permeability defects", "Imipenem Infections", "membrane alterations", "link membrane permeability", "vivo evolution", "G 7", "efflux pumps"], "article_id"=>1554207, "categories"=>["Uncategorised"], "users"=>["Nadège Philippe", "Laure Maigre", "Sébastien Santini", "Elizabeth Pinet", "Jean-Michel Claverie", "Anne-Véronique Davin-Régli", "Jean-Marie Pagès", "Muriel Masi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0138828.t003", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Features_of_the_G7_genome_/1554207", "title"=>"Features of the G7 genome.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-23 03:54:05"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"31", "full-text"=>"23", "pdf"=>"22", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"64", "full-text"=>"68", "pdf"=>"17", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"52", "full-text"=>"68", "pdf"=>"17", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"33", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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