Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis
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{"title"=>"Nanocurcumin prevents hypoxia induced stress in primary human ventricular cardiomyocytes by maintaining mitochondrial homeostasis", "type"=>"journal", "authors"=>[{"first_name"=>"Sarita", "last_name"=>"Nehra", "scopus_author_id"=>"56196830800"}, {"first_name"=>"Varun", "last_name"=>"Bhardwaj", "scopus_author_id"=>"56197149900"}, {"first_name"=>"Lilly", "last_name"=>"Ganju", "scopus_author_id"=>"6603372091"}, {"first_name"=>"Deepika", "last_name"=>"Saraswat", "scopus_author_id"=>"55886416100"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"26406246", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0139121", "issn"=>"19326203", "scopus"=>"2-s2.0-84947272681", "pui"=>"606879091", "sgr"=>"84947272681"}, "id"=>"750629ae-44d8-38a5-a570-74ab51389f15", "abstract"=>"Hypoxia induced oxidative stress incurs pathophysiological changes in hypertrophied cardiomyocytes by promoting translocation of p53 tomitochondria.Here,we investigate the cardio-protective efficacy of nanocurcumin in protecting primary human ventricular cardiomyo- cytes (HVCM) fromhypoxia induced damages.Hypoxia induced hypertrophywas confirmed by FITC-phenylalanine uptake assay, atrial natriuretic factor (ANF) levels and cell size mea- surements.Hypoxia induced translocation of p53was investigated by using mitochondrial membrane permeability transition pore blocker cyclosporin A (blocks entry of p53 to mitochon- dria) and confirmed bywestern blot and immunofluorescence. Mitochondrial damage in hyper- trophiedHVCMcellswas evaluated by analysing bio-energetic, anti-oxidant andmetabolic function and substrate switching formlipids to glucose.Nanocurcumin prevented translocation of p53 tomitochondria by stabilizingmitochondrial membrane potential and de-stressed hypertrophiedHVCMcells by significant restoration in lactate, acetyl-coenzyme A, pyruvate and glucose content alongwith lactate dehydrogenase (LDH) and 5' adenosinemonophos- phate-activated protein kinase (AMPKα) activity. Significant restoration in glucose andmodu- lation ofGLUT-1 andGLUT-4 levels confirmed that nanocurcuminmediated prevention of substrate switching.Nanocurcumin prevented of mitochondrial stress as confirmed by c-fos/c- jun/p53 signalling. The data indicates decrease in p-300 histone acetyl transferase (HAT) mediated histone acetylation andGATA-4 activation as pharmacological targets of nanocurcu- min in preventing hypoxia induced hypertrophy. The study provides an insight into propitious therapeutic", "link"=>"http://www.mendeley.com/research/nanocurcumin-prevents-hypoxia-induced-stress-primary-human-ventricular-cardiomyocytes-maintaining-mi", "reader_count"=>9, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2290140"], "description"=>"<p>(A) Hypoxia insult disrupted ΔΨ<sub>m</sub> as early as 1 h of exposure caused in HVCM cells as depicted by FACS. (B) Curcumin treatment did not show any significant improvement in ΔΨ<sub>m</sub> till 6 h of hypoxia and maximum protection was observed by 24 h of hypoxia. (C) Nanocurcumin treatment significantly restored the ΔΨ<sub>m</sub> by 3 h of hypoxia than curcumin treated cells and maximum restoration was achieved by 24 h of hypoxia.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557504, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g005", "stats"=>{"downloads"=>2, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_nanocurcumin_on_m_in_HVCM_cells_under_hypoxia_/1557504", "title"=>"Effect of nanocurcumin on ΔΨ<sub>m</sub> in HVCM cells under hypoxia:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290143"], "description"=>"<p>(A) MnSOD activity showed gradual decline with increment of duration of hypoxia as shown by fluorescent staining. (B) Curcumin treatment did not show significant restoration in MnSOD activity when compared to hypoxia control cells. (C) Treatment with nanocurcumin restored MnSOD activity in time dependent manner. Nanocurcumin significantly restored MnSOD activity in HVCM cells under hypoxia than curcumin treated cells under hypoxia when compared to cells exposed to hypoxia only. Random fields were captured by fluorescent microscope at 40 X magnification. At least 70 images were taken for each group and fluorescence intensity was quantized by using ImageJ software. Bar represents 10 μm.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557507, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g006", "stats"=>{"downloads"=>5, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_restored_MnSOD_levels_cells_under_hypoxia_/1557507", "title"=>"Nanocurcumin restored MnSOD levels cells under hypoxia:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290146"], "description"=>"<p>Translocation of p53 in mitochondria (mito) in HVCM cells was time-dependent and initiated within1 h of hypoxia. Nanocurcumin prevented p53 translocation to mitochondria in HVCM cells under hypoxia better than curcumin (A, B). Also, enhancement in levels of p53 (Red) and mtHsp70 (Green) was observed after hypoxia insult in HVCM cells after 24 h (C). Nanocurcumin treatment down-regulated p53 and mtHsp70 levels better than curcumin treated cells under hypoxia. Bar represents 10 μm.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557510, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g007", "stats"=>{"downloads"=>5, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_prevents_hypoxia_induced_mitochondrial_translocation_of_p53_in_HVCM_cells_/1557510", "title"=>"Nanocurcumin prevents hypoxia induced mitochondrial translocation of p53 in HVCM cells:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290148"], "description"=>"<p>Hypoxia insult prevented c-Fos and c-Jun expression in HVCM cells upto 24 h of hypoxia and thus promoted translocation of p53 to mitochondria. Treatment of HVCM cells with nanocurcumin promoted c-Fos accumulation as early as 1 h of hypoxia and prevented p53 mediated cell-death whereas curcumin treated cells showed significant up-regulation of c-Fos and c-Jun by 6–12 h of treatment under hypoxia showing that nanocurcumin is frequently available to the cells compared to curcumin. Nanocurcumin treated cells showed higher expression of negative regulators of p53 accumulation, i.e. c-Jun and c-Fos in HVCM cells under hypoxia, depicting that improved bio-availability and stability of nanocurcumin prevents hypoxia induced damage in cardiomyocytes.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557512, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g008", "stats"=>{"downloads"=>1, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_imparts_cardio_protection_in_HVCM_cells_by_activation_of_c_Jun_c_Fos_/1557512", "title"=>"Nanocurcumin imparts cardio-protection in HVCM cells by activation of c-Jun/c-Fos:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290149"], "description"=>"<p>Hypoxia insult caused severe decline in ATP levels and increase in p-AMPKα activity (A) increase in acetyl co-A and pyruvate concentrations (B). Also, there was observed in increase in lactate content and LDH activity in HVCM cells (C) along with glucose uptake (D) in HVCM cells under hypoxia, depicting that hypoxia induced energy deficit in cardiomyocytes. This was further confirmed by western blots of GLUT-1 and GLU4 (E, F). Nanocurcumin treatment restored the cellular ATP levels and p-AMPKα activity by 24 h of hypoxia exposure to normal levels than curcumin treated cells when compared to cells exposed to hypoxia only. Nanocurcumin treated cells showed restoration in acetyl co-A and pyruvate concentrations, lactate content and LDH activity along with glucose uptake and expression levels of glucose transporters, whereas curcumin treated cells did not show significant restoration. Values are mean ± SD, significant values represented as <sup>@</sup>p≤0.05 <i>vs</i> normoxia, <sup>@@</sup> p≤0.01 <i>vs</i> normoxia *p≤0.05 <i>vs</i> hypoxia and <sup>#</sup>p≤0.01 <i>vs</i> hypoxia. Non-significant changes are designated as ns.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557513, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g009", "stats"=>{"downloads"=>3, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_prevents_hypoxia_induced_mitochondrial_stress_in_HVCM_cells_/1557513", "title"=>"Nanocurcumin prevents hypoxia induced mitochondrial stress in HVCM cells:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290151"], "description"=>"<p>Hypoxia stress disturbed cellular redox balance as depicted by increase in ROS leakage and lipid peroxidation and concomitant decrease in GSH/GSSG content, xanthine oxidase activity and MnSOD activity. Nanocurcumin treatment showed significant improvement in redox status when compared to curcumin treated cells under hypoxia and cells exposed to hypoxia only. No significant change in redox status was observed in cells treated with curcumin or nanocurcumin under normoxia. Values are mean ± SD, significant values represented as <sup>@</sup>p≤0.05 <i>vs</i> normoxia, <sup>#</sup>p≤0.01 <i>vs</i> hypoxia, *p≤0.05 <i>vs</i> hypoxia.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557515, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g010", "stats"=>{"downloads"=>3, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_protects_HVCM_cells_by_restoring_oxidative_balance_/1557515", "title"=>"Nanocurcumin protects HVCM cells by restoring oxidative balance:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290156", "https://ndownloader.figshare.com/files/2290157", "https://ndownloader.figshare.com/files/2290158", "https://ndownloader.figshare.com/files/2290159", "https://ndownloader.figshare.com/files/2290160"], "description"=>"<div><p>Hypoxia induced oxidative stress incurs pathophysiological changes in hypertrophied cardiomyocytes by promoting translocation of p53 to mitochondria. Here, we investigate the cardio-protective efficacy of nanocurcumin in protecting primary human ventricular cardiomyocytes (HVCM) from hypoxia induced damages. Hypoxia induced hypertrophy was confirmed by FITC-phenylalanine uptake assay, atrial natriuretic factor (ANF) levels and cell size measurements. Hypoxia induced translocation of p53 was investigated by using mitochondrial membrane permeability transition pore blocker cyclosporin A (blocks entry of p53 to mitochondria) and confirmed by western blot and immunofluorescence. Mitochondrial damage in hypertrophied HVCM cells was evaluated by analysing bio-energetic, anti-oxidant and metabolic function and substrate switching form lipids to glucose. Nanocurcumin prevented translocation of p53 to mitochondria by stabilizing mitochondrial membrane potential and de-stressed hypertrophied HVCM cells by significant restoration in lactate, acetyl-coenzyme A, pyruvate and glucose content along with lactate dehydrogenase (LDH) and 5' adenosine monophosphate-activated protein kinase (AMPKα) activity. Significant restoration in glucose and modulation of GLUT-1 and GLUT-4 levels confirmed that nanocurcumin mediated prevention of substrate switching. Nanocurcumin prevented of mitochondrial stress as confirmed by c-fos/c-jun/p53 signalling. The data indicates decrease in p-300 histone acetyl transferase (HAT) mediated histone acetylation and GATA-4 activation as pharmacological targets of nanocurcumin in preventing hypoxia induced hypertrophy. The study provides an insight into propitious therapeutic effects of nanocurcumin in cardio-protection and usability in clinical applications.</p></div>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557520, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0139121.s001", "https://dx.doi.org/10.1371/journal.pone.0139121.s002", "https://dx.doi.org/10.1371/journal.pone.0139121.s003", "https://dx.doi.org/10.1371/journal.pone.0139121.s004", "https://dx.doi.org/10.1371/journal.pone.0139121.s005"], "stats"=>{"downloads"=>7, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_Prevents_Hypoxia_Induced_Stress_in_Primary_Human_Ventricular_Cardiomyocytes_by_Maintaining_Mitochondrial_Homeostasis_/1557520", "title"=>"Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290130"], "description"=>"<p>(A) Hypoxia insult caused decline in cell viability (20%) compared to normoxia control cells by 24 h. (B) Nanocurcumin treatment at 500 ng/ml significantly improved cellular viability (89%) by 24 h of hypoxia exposure and confirmed by FACS. Curcumin treatment did not show significant improvement in cellular viability at any time point. No significant change in cell viability was observed in nanocurcumin or curcumin treated cells under normoxia. Nanocurcumin treatment at 500 ng/ml was used for further experiments. Values are mean ± SD, significant values represented as <sup>@@</sup>p≤0.01 <i>vs</i> normoxia and <sup>#</sup>p≤0.01 <i>vs</i> hypoxia. Non-significant changes are designated as ns.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557496, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g001", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_improves_cellular_viability_under_hypoxia_/1557496", "title"=>"Nanocurcumin improves cellular viability under hypoxia:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290134"], "description"=>"<p>(A, B) Fluorescent staining of HVCM cells (60X) with actin filaments (green) and nuclei (blue) under various experimental groups showed increase in cell size in hypoxia exposed cells compared to normoxia controls. (C) Increment in FITC-phenylalanine uptake was observed in cells exposed to hypoxia compared to normoxia control. (D) Increase in ANF levels were observed within 1 h of hypoxia and reached maximum by 24 h of hypoxia. Treatment with nanocurcumin significantly restored the cell size and FITC-phenylalanine uptake under hypoxia when compared to curcumin treated cells and cells exposed to hypoxia only. Nanocurcumin treated cells showed tremendous restoration in ANF levels under hypoxia at all the time points compared to curcumin treated cells. No significant changes in ANF levels, cell size or FITC-phenylalanine uptake was observed in cells exposed to normoxia after treatment with nanocurcumin or curcumin. Values are mean ± SD, significant values represented as <sup>@@</sup> p≤0.01 <i>vs</i> normoxia, *p≤0.05 <i>vs</i> hypoxia, <sup>#</sup>p≤0.01 <i>vs</i> hypoxia, <sup>@</sup> p≤0.005 <i>vs</i> hypoxia and <sup>$ $</sup> p≤0.001 <i>vs</i> hypoxia. Bar represents 50 μm.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557500, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g002", "stats"=>{"downloads"=>3, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nanocurcumin_prevents_hypoxia_induced_hypertrophy_in_HVCM_cells_/1557500", "title"=>"Nanocurcumin prevents hypoxia induced hypertrophy in HVCM cells:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290135"], "description"=>"<p>Histone acetylation by p-300 was up-regulated with a corresponding decline in HDAC activity in cardiomyocytes under hypoxia depicting that hypoxia induced hypertrophy dependent upon p-300 HAT and HDAC activity (A and C). Nanocurcumin treatment significantly down-regulated p-300 HAT and up-regulated HDAC activity in HVCM cells under hypoxia compared to cells exposed to hypoxia only. This was further confirmed by western blot analysis of GATA-4 and p-GATA-4 levels along with acetylated histone 3 and 4 (B). However, curcumin treatment did not prevent histone acetylation under hypoxia. Also, nanocurcumin or curcumin treated cells did not show change in p-300 HAT activity under normoxia. Values are mean ± SD, significant values represented as <sup>@@</sup> p≤0.01 <i>vs</i> normoxia, *p≤0.05 <i>vs</i> hypoxia and <sup>#</sup>p≤0.01 <i>vs</i> hypoxia. Non-significant changes are depicted as ns. Scale bar represents 50μm.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557501, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g003", "stats"=>{"downloads"=>4, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_nanocurcumin_on_p_300_HAT_and_HDAC_activities_in_HVCM_cells_under_hypoxia_/1557501", "title"=>"Effect of nanocurcumin on p-300 HAT and HDAC activities in HVCM cells under hypoxia:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/2290136"], "description"=>"<p>The dependency of translocation of p53 to mitochondria on induction of hypertrophy in HVCM cells under hypoxia was estimated by blocking entry of p53 to mitochondria by treatment with CsA. Treatment of HVCM cells with CsA prevented p53 translocation to mitochondria but did not affect ANF levels in HVC cells exposed to hypoxia for 24 h. However, treatment HVCM cells with nanocurcumin significantly reduced p53 translocation to mitochondria and down-regulated ANF levels. Values are mean ± SD, significant values represented as <sup>@@</sup>p≤0.01 <i>vs</i> normoxia, <sup>#</sup>p≤0.01 <i>vs</i> hypoxia, *p≤0.05 <i>vs</i> hypoxia.</p>", "links"=>[], "tags"=>["Hypoxia Induced Stress", "cardiomyocyte", "hypoxia", "p 53", "substrate", "mitochondrial membrane permeability transition pore blocker cyclosporin", "nanocurcumin", "Mitochondrial Homeostasis Hypoxia", "ldh", "gata", "HVCM cells", "histone acetyl transferase", "atrial natriuretic factor", "ampk", "anf", "Primary Human Ventricular Cardiomyocytes", "glut", "glucose", "hypertrophy", "cell size measurements", "translocation", "restoration", "stress", "lactate"], "article_id"=>1557502, "categories"=>["Biological Sciences"], "users"=>["Sarita Nehra", "Varun Bhardwaj", "Lilly Ganju", "Deepika Saraswat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139121.g004", "stats"=>{"downloads"=>2, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hypoxia_induced_hypertrophy_is_independent_of_translocation_of_p53_to_mitochondria_/1557502", "title"=>"Hypoxia induced hypertrophy is independent of translocation of p53 to mitochondria:", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-25 04:46:42"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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