Subcellular Partitioning of Protein Tyrosine Phosphatase 1B to the Endoplasmic Reticulum and Mitochondria Depends Sensitively on the Composition of Its Tail Anchor
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Subcellular partitioning of protein tyrosine Phosphatase 1b to the endoplasmic reticulum and mitochondria Dependssensitively on the composition of its tail anchor", "type"=>"journal", "authors"=>[{"first_name"=>"Julia", "last_name"=>"Fueller", "scopus_author_id"=>"56964214200"}, {"first_name"=>"Mikhail V.", "last_name"=>"Egorov", "scopus_author_id"=>"56963649300"}, {"first_name"=>"Kirstin A.", "last_name"=>"Walther", "scopus_author_id"=>"12807836400"}, {"first_name"=>"Ola", "last_name"=>"Sabet", "scopus_author_id"=>"35107902300"}, {"first_name"=>"Jana", "last_name"=>"Mallah", "scopus_author_id"=>"55067457100"}, {"first_name"=>"Markus", "last_name"=>"Grabenbauer", "scopus_author_id"=>"7801540092"}, {"first_name"=>"Ali", "last_name"=>"Kinkhabwala", "scopus_author_id"=>"36811170400"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84947219200", "pmid"=>"26431424", "sgr"=>"84947219200", "doi"=>"10.1371/journal.pone.0139429", "issn"=>"19326203", "pui"=>"606879422"}, "id"=>"99ecefad-8ac7-35ef-88c3-185972c868c4", "abstract"=>"The canonical protein tyrosine phosphatase PTP1B is an important regulator of diverse cellular signaling networks. PTP1B has long been thought to exert its influence solely from its perch on the endoplasmic reticulum (ER); however, an additional subpopulation of PTP1B has recently been detected in mitochondria extracted from rat brain tissue. Here, we show that PTP1B's mitochondrial localization is general (observed across diverse mammalian cell lines) and sensitively dependent on the transmembrane domain length, C-terminal charge and hydropathy of its short (≤35 amino acid) tail anchor. Our electron microscopy of specific DAB precipitation revealed that PTP1B localizes via its tail anchor to the outer mitochondrial membrane (OMM), with fluorescence lifetime imaging microscopy establishing that this OMM pool contributes to the previously reported cytoplasmic interaction of PTP1B with endocytosed epidermal growth factor receptor. We additionally examined the mechanism of PTP1B's insertion into the ER membrane through heterologous expression of PTP1B's tail anchor in wild-type yeast and yeast mutants of major conserved ER insertion pathways: In none of these yeast strains was ER targeting significantly impeded, providing in vivo support for the hypothesis of spontaneous membrane insertion (as previously demonstrated in vitro). Further functional elucidation of the newly recognized mitochondrial pool of PTP1B will likely be important for understanding its complex roles in cellular responses to external stimuli, cell proliferation and diseased states.", "link"=>"http://www.mendeley.com/research/subcellular-partitioning-protein-tyrosine-phosphatase-1b-endoplasmic-reticulum-mitochondria-dependss", "reader_count"=>17, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>9, "Neuroscience"=>1, "Physics and Astronomy"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84947219200"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84947219200?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84947219200&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84947219200&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84947219200", "dc:identifier"=>"SCOPUS_ID:84947219200", "eid"=>"2-s2.0-84947219200", "dc:title"=>"Subcellular partitioning of protein tyrosine Phosphatase 1b to the endoplasmic reticulum and mitochondria Dependssensitively on the composition of its tail anchor", "dc:creator"=>"Fueller J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"10", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-10-02", "prism:coverDisplayDate"=>"2 October 2015", "prism:doi"=>"10.1371/journal.pone.0139429", "citedby-count"=>"9", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Max Planck Institut für molekulare Physiologie", "affiliation-city"=>"Dortmund", "affiliation-country"=>"Germany"}, {"@_fa"=>"true", "affilname"=>"Universität Heidelberg", "affiliation-city"=>"Heidelberg", "affiliation-country"=>"Germany"}], "pubmed-id"=>"26431424", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0139429", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0139429", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Counter

  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"56", "xml_views"=>"11", "html_views"=>"437"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"3", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2297057"], "description"=>"<p>See text for further description.</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562924, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Wild_type_and_mutant_PTP1B_tail_anchor_isoforms_examined_in_this_study_/1562924", "title"=>"Wild-type and mutant PTP1B tail anchor isoforms examined in this study.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297044"], "description"=>"<p>Mammalian cells were fixed with PFA and immunostained for endogenous PTP1B using an anti-PTP1B (Ab-1) mouse monoclonal primary antibody (Calbiochem) and a chicken anti-mouse secondary antibody conjugated with Alexa488 (Invitrogen). Mitochondria were stained with MitoTracker Red CMXRos (Invitrogen). COS-7, BJ Fibroblast, HeLa, MCF7, MDCK and HepG2 cells were visualized with confocal microscopy. Representative mitochondria are marked with arrows. Mitochondrial targeting of PTP1B was easier to assess in the cells with a flatter morphology towards the top of the figure as compared with the more compact cells towards the bottom. Scale bars: 20 μm.</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562915, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g001", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mitochondrial_localization_of_endogenous_PTP1B_in_multiple_mammalian_cell_lines_/1562915", "title"=>"Mitochondrial localization of endogenous PTP1B in multiple mammalian cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297061"], "description"=>"<p>Our experimental localization results for different tail isoforms in COS-7 cells and yeast cells are summarized. We have shown that the wild-type localization of PTP1Btail to the mitochondria and ER (Mito/ER) is highly sensitive to changes in these three factors (see text and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139429#pone.0139429.s008\" target=\"_blank\">S8 Fig</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139429#pone.0139429.s009\" target=\"_blank\">S9 Fig</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139429#pone.0139429.s010\" target=\"_blank\">S10 Fig</a>).</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562929, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g006", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tail_anchor_targeting_is_largely_dictated_by_three_different_factors_TMD_length_C_terminal_charge_and_hydropathy_/1562929", "title"=>"Tail anchor targeting is largely dictated by three different factors: TMD length, C-terminal charge and hydropathy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297060"], "description"=>"<p>We used an aggregate-formation assay to examine the impact of deletion of the various insertion pathway components in yeast. Aggregate formation in strains expressing tail anchors from Ysy6 and Sec22 upon deletion of the GET pathway component Get2 was used as a control. Comparing wild-type with Get2∆ deletion strains that expressed yemCitrine-Ysy6tail or yemCitrine-Sec22tail, we observed visible aggregates in a fraction of the Get2∆ cells that were consistent with previous findings (though the bulk of these proteins still managed to insert properly). We observed similar aggregates in cells expressing yemCitrine-PTP1Btail. Slightly more aggregates were observed in Get3∆ cells and significantly fewer in the Sgt2∆ cells of this strain. A strain in which both Get2 and Get1 were deleted was similar to the Get2∆ deletion strain. The green box indicates all strains in which one or more GET pathway components were deleted. Deletion of the α subunit of the SRP receptor (Srp101) led to a much larger cell phenotype (blue box), but no change in the ER and vacuolar partitioning of the PTP1B tail anchor. Deletion of Sec72 (yellow box), an essential factor of the Sec62/63 pathway, also did not alter the localization of the PTP1B tail anchor. Deletion of Sec72 in the Get2∆/Get1∆ strain did not further alter the localization of the PTP1B tail anchor beyond that observed in the Get2∆/Get1∆ strain. Finally, deletion of the chaperones Apj1 and Ydj1 (as well as the farnesyl transferase of Ydj1, Ram1) did not alter PTP1B’s subcellular localization (red box). The intensity scale shown at the bottom right and indicating photon counts/pixel is applicable to all images, as is the scale bar shown in the bottom right panel. Scale bar: 10 μm.</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562928, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Systematic_study_of_the_potential_pathways_responsible_for_the_subcellular_targeting_of_the_tail_anchor_of_PTP1B_in_yeast_/1562928", "title"=>"Systematic study of the potential pathways responsible for the subcellular targeting of the tail anchor of PTP1B in yeast.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297054"], "description"=>"<p>(A) High magnification EM image of several mitochondria expressing mTurquoise-APEX-PTP1B in COS-7 cells. The pronounced DAB precipitate was locally concentrated at the OMM (black arrow) and was notably absent from the intracristal spaces and matrix. Strong staining is also observed along ER subregions in direct apposition to mitochondria and therefore consistent with ER MAM sites (white arrows). (B) Similarly strong DAB precipitation was observed at the OMM of a COS-7 cell expressing mTFP1-APEX-PTP1Btail (black arrow). (C) DAB precipitation was observed at the OMM of a COS-7 cell expressing Tom20-APEX-mTurquoise (black arrow). (D) Image of unlabeled mitochondria from an untransfected COS-7 cell serves as a negative control. The black arrowhead indicates the absence of the labeling of the mitochondrial interior. Scale bars: 500 nm.</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562922, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EM_imaging_of_subcellular_PTP1B_partitioning_/1562922", "title"=>"EM imaging of subcellular PTP1B partitioning.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297101", "https://ndownloader.figshare.com/files/2297102", "https://ndownloader.figshare.com/files/2297103", "https://ndownloader.figshare.com/files/2297104", "https://ndownloader.figshare.com/files/2297105", "https://ndownloader.figshare.com/files/2297106", "https://ndownloader.figshare.com/files/2297107", "https://ndownloader.figshare.com/files/2297108", "https://ndownloader.figshare.com/files/2297109", "https://ndownloader.figshare.com/files/2297110", "https://ndownloader.figshare.com/files/2297111", "https://ndownloader.figshare.com/files/2297112", "https://ndownloader.figshare.com/files/2297113", "https://ndownloader.figshare.com/files/2297114", "https://ndownloader.figshare.com/files/2297115", "https://ndownloader.figshare.com/files/2297116", "https://ndownloader.figshare.com/files/2297117", "https://ndownloader.figshare.com/files/2297118", "https://ndownloader.figshare.com/files/2297119", "https://ndownloader.figshare.com/files/2297120"], "description"=>"<div><p>The canonical protein tyrosine phosphatase PTP1B is an important regulator of diverse cellular signaling networks. PTP1B has long been thought to exert its influence solely from its perch on the endoplasmic reticulum (ER); however, an additional subpopulation of PTP1B has recently been detected in mitochondria extracted from rat brain tissue. Here, we show that PTP1B’s mitochondrial localization is general (observed across diverse mammalian cell lines) and sensitively dependent on the transmembrane domain length, C-terminal charge and hydropathy of its short (≤35 amino acid) tail anchor. Our electron microscopy of specific DAB precipitation revealed that PTP1B localizes via its tail anchor to the outer mitochondrial membrane (OMM), with fluorescence lifetime imaging microscopy establishing that this OMM pool contributes to the previously reported cytoplasmic interaction of PTP1B with endocytosed epidermal growth factor receptor. We additionally examined the mechanism of PTP1B’s insertion into the ER membrane through heterologous expression of PTP1B’s tail anchor in wild-type yeast and yeast mutants of major conserved ER insertion pathways: In none of these yeast strains was ER targeting significantly impeded, providing in vivo support for the hypothesis of spontaneous membrane insertion (as previously demonstrated in vitro). Further functional elucidation of the newly recognized mitochondrial pool of PTP1B will likely be important for understanding its complex roles in cellular responses to external stimuli, cell proliferation and diseased states.</p></div>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562956, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0139429.s001", "https://dx.doi.org/10.1371/journal.pone.0139429.s002", "https://dx.doi.org/10.1371/journal.pone.0139429.s003", "https://dx.doi.org/10.1371/journal.pone.0139429.s004", "https://dx.doi.org/10.1371/journal.pone.0139429.s005", "https://dx.doi.org/10.1371/journal.pone.0139429.s006", "https://dx.doi.org/10.1371/journal.pone.0139429.s007", "https://dx.doi.org/10.1371/journal.pone.0139429.s008", "https://dx.doi.org/10.1371/journal.pone.0139429.s009", "https://dx.doi.org/10.1371/journal.pone.0139429.s010", "https://dx.doi.org/10.1371/journal.pone.0139429.s011", "https://dx.doi.org/10.1371/journal.pone.0139429.s012", "https://dx.doi.org/10.1371/journal.pone.0139429.s013", "https://dx.doi.org/10.1371/journal.pone.0139429.s014", "https://dx.doi.org/10.1371/journal.pone.0139429.s015", "https://dx.doi.org/10.1371/journal.pone.0139429.s016", "https://dx.doi.org/10.1371/journal.pone.0139429.s017", "https://dx.doi.org/10.1371/journal.pone.0139429.s018", "https://dx.doi.org/10.1371/journal.pone.0139429.s019", "https://dx.doi.org/10.1371/journal.pone.0139429.s020"], "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Subcellular_Partitioning_of_Protein_Tyrosine_Phosphatase_1B_to_the_Endoplasmic_Reticulum_and_Mitochondria_Depends_Sensitively_on_the_Composition_of_Its_Tail_Anchor_/1562956", "title"=>"Subcellular Partitioning of Protein Tyrosine Phosphatase 1B to the Endoplasmic Reticulum and Mitochondria Depends Sensitively on the Composition of Its Tail Anchor", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-10-02 03:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/2297049"], "description"=>"<p>Confocal images of COS-7 cells expressing mCitrine-PTP1B along with the mitochondrial marker Tom20-mTagBFP. The mCitrine-PTP1B chimera localized to the general ER (arrowhead) and at a higher local concentration to the mitochondria (arrow). Scale bar: 20 μm.</p>", "links"=>[], "tags"=>["PTP 1B tail anchor", "PTP 1B mitochondrial localization", "tail anchor", "PTP 1B localizes", "endocytosed epidermal growth factor receptor", "transmembrane domain length", "Mitochondria Depends Sensitively", "PTP 1B", "canonical protein tyrosine phosphatase PTP 1B", "ER insertion pathways", "PTP 1B insertion", "fluorescence lifetime imaging microscopy", "omm", "dab", "protein tyrosine phosphatase 1 B", "rat brain tissue"], "article_id"=>1562917, "categories"=>["Uncategorised"], "users"=>["Julia Fueller", "Mikhail V. Egorov", "Kirstin A. Walther", "Ola Sabet", "Jana Mallah", "Markus Grabenbauer", "Ali Kinkhabwala"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0139429.g002", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PTP1B_localizes_to_the_mitochondria_in_COS_7_cells_/1562917", "title"=>"PTP1B localizes to the mitochondria in COS-7 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-02 03:16:46"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"27", "full-text"=>"24", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"19", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"40", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"22", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts