MreC and MreD Proteins Are Not Required for Growth of Staphylococcus aureus
Publication Date
October 15, 2015
Journal
PLOS ONE
Authors
Andreia C. Tavares, Pedro B. Fernandes, Rut Carballido López & Mariana G. Pinho
Volume
10
Issue
10
Pages
e0140523
DOI
https://dx.plos.org/10.1371/journal.pone.0140523
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0140523
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26470021
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607420
Europe PMC
http://europepmc.org/abstract/MED/26470021
Web of Science
000363184600071
Scopus
84949058405
Mendeley
http://www.mendeley.com/research/mrec-mred-proteins-not-required-growth-staphylococcus-aureus
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2361664"], "description"=>"<p>(A) Growth curves for inducible mutant COL<i>mreCD</i>i in TSB medium in the presence (Δ) and absence (●) of 0.5 mM IPTG (a). When the culture without IPTG reached OD<sub>600nm</sub> ≈ 1, a sample was used to inoculate fresh media (b) with (□) and without (ӿ) 0.5 mM IPTG. (B and C) Growth curves for COL, COLΔ<i>mreC</i>, COLΔ<i>mreD</i> and COLΔ<i>mreCD</i> in TSB rich medium (B) and SSM9PR minimal medium (C). Mutant strains have a growth rate similar to the parental strain, with a duplication time of approximately 41 min in TSB and 81 min in SSM9PR.</p>", "links"=>[], "tags"=>["cell cycle control", "Staphylococcus aureus", "cell wall", "actin homologue MreB", "cell division", "lack MreB homologues", "transmembrane proteins MreC", "pathogen Staphylococcus aureus", "division septa", "cell wall synthesis", "cell volume", "stress agents", "MreD Proteins", "Cell Morphology", "cell shape determination", "influence peptidoglycan composition", "cell viability"], "article_id"=>1577049, "categories"=>["Uncategorised"], "users"=>["Andreia C. Tavares", "Pedro B. Fernandes", "Rut Carballido-López", "Mariana G. Pinho"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0140523.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Depletion_of_MreC_or_MreD_does_not_impair_S_aureus_COL_growth_/1577049", "title"=>"Depletion of MreC or MreD does not impair <i>S</i>. <i>aureus</i> COL growth.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-15 03:31:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/2361665"], "description"=>"<p><i>S. aureus</i> COL cells expressing sGFP fused to the N-terminal of MreC (top) or MreD (bottom) were grown in TSB supplemented with 0.2 mM IPTG and observed by SR-SIM. Left panels show differential interference contrast (DIC) images and right panels show fluorescence signal of sGFP fusion proteins. Scale bars, 0.5 μm.</p>", "links"=>[], "tags"=>["cell cycle control", "Staphylococcus aureus", "cell wall", "actin homologue MreB", "cell division", "lack MreB homologues", "transmembrane proteins MreC", "pathogen Staphylococcus aureus", "division septa", "cell wall synthesis", "cell volume", "stress agents", "MreD Proteins", "Cell Morphology", "cell shape determination", "influence peptidoglycan composition", "cell viability"], "article_id"=>1577050, "categories"=>["Uncategorised"], "users"=>["Andreia C. Tavares", "Pedro B. Fernandes", "Rut Carballido-López", "Mariana G. Pinho"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0140523.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MreC_and_MreD_are_enriched_at_the_division_septum_/1577050", "title"=>"MreC and MreD are enriched at the division septum.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-15 03:31:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/2361666"], "description"=>"<p>HPLC profiles of peptidoglycan muropeptides from <i>S</i>. <i>aureus</i> COL, COLΔ<i>mreC</i>, COLΔ<i>mreD</i> and COLΔ<i>mreCD</i>.</p>", "links"=>[], "tags"=>["cell cycle control", "Staphylococcus aureus", "cell wall", "actin homologue MreB", "cell division", "lack MreB homologues", "transmembrane proteins MreC", "pathogen Staphylococcus aureus", "division septa", "cell wall synthesis", "cell volume", "stress agents", "MreD Proteins", "Cell Morphology", "cell shape determination", "influence peptidoglycan composition", "cell viability"], "article_id"=>1577051, "categories"=>["Uncategorised"], "users"=>["Andreia C. Tavares", "Pedro B. Fernandes", "Rut Carballido-López", "Mariana G. Pinho"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0140523.g003", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Profiles_of_peptidoglycan_muropeptides_remain_unaltered_in_the_absence_of_MreC_or_MreD_/1577051", "title"=>"Profiles of peptidoglycan muropeptides remain unaltered in the absence of MreC or MreD.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-15 03:31:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/2361669"], "description"=>"<p>(A) COL, COLΔ<i>mreC</i>, COLΔ<i>mreD</i> and COLΔ<i>mreCD</i> were imaged by Superresolution Structured Illumination Microscopy (SR-SIM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). For the SR-SIM images, cell wall and DNA were labeled with fluorescent vancomycin (Van-FL) and Hoechst 33342, respectively. Scale bars for SR-SIM and SEM images 0.5 μm and for TEM images 0.2 μm (B) Fraction of the cell cycle spent in Phase 1 (blue, before initiation of septum synthesis), Phase 2 (red, septum synthesis) and Phase 3 (green, after closure of the septum, before the two daughter cells split) [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140523#pone.0140523.ref020\" target=\"_blank\">20</a>]. Cell cycle progresses clockwise. From the inner to the outer circle the values correspond to COL, COLΔ<i>mreC</i>, COLΔ<i>mreD</i> and COLΔ<i>mreCD</i>. Exponentially growing cultures were labeled with the membrane dye Nile Red and the percentage of cells in each phase of the cell cycle was determined. (C) Cellular volume distributions during each phase of the cell cycle. Cells were labeled with Nile Red and imaged by SR-SIM. The volume was calculated by approximation of the cellular shape to a prolate spheroid. n = 60 for each phase. Statistical analysis was performed using the unpaired <i>t</i> test and p-values were >0.05 in all cases compared to the parental strain COL, except for Phase 1 COLΔ<i>mreD</i> (<i>p</i> = 0.013) and for COLΔ<i>mreCD</i> (<i>p</i> = 0.014).</p>", "links"=>[], "tags"=>["cell cycle control", "Staphylococcus aureus", "cell wall", "actin homologue MreB", "cell division", "lack MreB homologues", "transmembrane proteins MreC", "pathogen Staphylococcus aureus", "division septa", "cell wall synthesis", "cell volume", "stress agents", "MreD Proteins", "Cell Morphology", "cell shape determination", "influence peptidoglycan composition", "cell viability"], "article_id"=>1577054, "categories"=>["Uncategorised"], "users"=>["Andreia C. Tavares", "Pedro B. Fernandes", "Rut Carballido-López", "Mariana G. Pinho"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0140523.g004", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Absence_of_MreC_or_MreD_has_no_effect_on_cell_morphology_cell_dimensions_or_cell_cycle_progression_/1577054", "title"=>"Absence of MreC or MreD has no effect on cell morphology, cell dimensions or cell cycle progression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-15 03:31:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/2361672", "https://ndownloader.figshare.com/files/2361673", "https://ndownloader.figshare.com/files/2361674", "https://ndownloader.figshare.com/files/2361675", "https://ndownloader.figshare.com/files/2361676", "https://ndownloader.figshare.com/files/2361677", "https://ndownloader.figshare.com/files/2361678", "https://ndownloader.figshare.com/files/2361679", "https://ndownloader.figshare.com/files/2361680", "https://ndownloader.figshare.com/files/2361681"], "description"=>"<div><p>The transmembrane proteins MreC and MreD are present in a wide variety of bacteria and are thought to be involved in cell shape determination. Together with the actin homologue MreB and other morphological elements, they play an essential role in the synthesis of the lateral cell wall in rod-shaped bacteria. In ovococcus, which lack MreB homologues, <i>mreCD</i> are also essential and have been implicated in peripheral cell wall synthesis. In this work we addressed the possible roles of MreC and MreD in the spherical pathogen <i>Staphylococcus aureus</i>. We show that MreC and MreD are not essential for cell viability and do not seem to affect cell morphology, cell volume or cell cycle control. MreC and MreD localize preferentially to the division septa, but do not appear to influence peptidoglycan composition, nor the susceptibility to different antibiotics and to oxidative and osmotic stress agents. Our results suggest that the function of MreCD in <i>S</i>. <i>aureus</i> is not critical for cell division and cell shape determination.</p></div>", "links"=>[], "tags"=>["cell cycle control", "Staphylococcus aureus", "cell wall", "actin homologue MreB", "cell division", "lack MreB homologues", "transmembrane proteins MreC", "pathogen Staphylococcus aureus", "division septa", "cell wall synthesis", "cell volume", "stress agents", "MreD Proteins", "Cell Morphology", "cell shape determination", "influence peptidoglycan composition", "cell viability"], "article_id"=>1577057, "categories"=>["Uncategorised"], "users"=>["Andreia C. Tavares", "Pedro B. Fernandes", "Rut Carballido-López", "Mariana G. Pinho"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0140523.s001", "https://dx.doi.org/10.1371/journal.pone.0140523.s002", "https://dx.doi.org/10.1371/journal.pone.0140523.s003", "https://dx.doi.org/10.1371/journal.pone.0140523.s004", "https://dx.doi.org/10.1371/journal.pone.0140523.s005", "https://dx.doi.org/10.1371/journal.pone.0140523.s006", "https://dx.doi.org/10.1371/journal.pone.0140523.s007", "https://dx.doi.org/10.1371/journal.pone.0140523.s008", "https://dx.doi.org/10.1371/journal.pone.0140523.s009", "https://dx.doi.org/10.1371/journal.pone.0140523.s010"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/MreC_and_MreD_Proteins_Are_Not_Required_for_Growth_of_Staphylococcus_aureus_/1577057", "title"=>"MreC and MreD Proteins Are Not Required for Growth of <i>Staphylococcus aureus</i>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-10-15 03:31:45"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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