Genotypic and Phenotypic Heterogeneity in Alicyclobacillus acidoterrestris: A Contribution to Species Characterization
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{"title"=>"Genotypic and phenotypic heterogeneity in alicyclobacillus acidoterrestris: A contribution to species characterization", "type"=>"journal", "authors"=>[{"first_name"=>"Antonio", "last_name"=>"Bevilacqua", "scopus_author_id"=>"9036356800"}, {"first_name"=>"Monica", "last_name"=>"Mischitelli", "scopus_author_id"=>"8914906500"}, {"first_name"=>"Valeria", "last_name"=>"Pietropaolo", "scopus_author_id"=>"7004104749"}, {"first_name"=>"Emanuela", "last_name"=>"Ciuffreda", "scopus_author_id"=>"55813876300"}, {"first_name"=>"Milena", "last_name"=>"Sinigaglia", "scopus_author_id"=>"6603854896"}, {"first_name"=>"Maria Rosaria", "last_name"=>"Corbo", "scopus_author_id"=>"7006723914"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"26484547", "sgr"=>"84949256956", "doi"=>"10.1371/journal.pone.0141228", "scopus"=>"2-s2.0-84949256956", "pui"=>"607132675", "issn"=>"19326203"}, "id"=>"a26f1132-aea7-3e30-9e72-ccde8d9b8b63", "abstract"=>"Alicyclobacillus acidoterrestris is the main cause of most spoilage problems in fruit juices and acidic products. Since soil borne species often contaminate fruit juices and do not need strict extreme requirements for survival, it is a great concern to investigate whether and how soil species could evolve from their ecological niches in microbial community to new environments as fruit juices. In this study, 23 isolates of thermo-acidophilic, spore-forming bacteria from soil were characterized by cultural and molecular methods. In addition, 2 strains isolated from a spoilage incident in pear juice were typed. Strains phenotyping showed that they could be grouped into 3 different clusters, and some isolates showed identical or quite similar patterns. Analyzing pH and temperature ranges for growth, the majority of strains were able to grow at values described for many species of Alicyclobacillus. Qualitative utilization of lysine, arginine and indole production from tryptophan revealed, for the first time, deamination of lysine and decarboxylation of arginine. Resistance to 5% NaCl as well as the ability to hydrolyze starch and gelatin, nitrate reduction, catalase and oxidase activities confirmed literature evidences. Examining of 16S rRNA, showed that isolates were divided into three blocks represented by effectively soil species and strains that are moving from soil to other possible growing source characterized by parameters that could strongly influence bacterial survival. RAPD PCR technique evidenced a great variability in banding patterns and, although it was not possible to obtain genotypically well-distinguished groups, it was feasible to appreciate genetic similarity between some strains. In conclusion, the investigation of a microbial community entails a combination of metagenomic and classic culture-dependent approaches to expand our knowledge about Alicyclobacillus and to look for new subspecies.", "link"=>"http://www.mendeley.com/research/genotypic-phenotypic-heterogeneity-alicyclobacillus-acidoterrestris-contribution-species-characteriz", "reader_count"=>8, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>2, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>2, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>6}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2366892"], "description"=>"<p>(The evolutionary history was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 0.79567517 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and are in the units of the number of base differences per site. The analysis involved 29 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 573 positions in the final dataset. Evolutionary analyses were conducted in MEGA6.).</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1580992, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g003", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evolutionary_relationships_of_taxa_/1580992", "title"=>"Evolutionary relationships of taxa.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366886"], "description"=>"<p>Clustering of isolated <i>A</i>. <i>acidoterrestris</i> strains considering some biochemical traits.</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1580986, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g001", "stats"=>{"downloads"=>6, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clustering_of_isolated_A_acidoterrestris_strains_considering_some_biochemical_traits_/1580986", "title"=>"Clustering of isolated <i>A</i>. <i>acidoterrestris</i> strains considering some biochemical traits.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366906"], "description"=>"<p>RAPD PCR products obtained amplifying chromosomal DNA of studied <i>A</i>. <i>acidoterrestris</i> strains as the template, primer F-64 was used.</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1581000, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g005", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RAPD_PCR_products_obtained_amplifying_chromosomal_DNA_of_studied_A_acidoterrestris_strains_as_the_template_primer_F_64_was_used_/1581000", "title"=>"RAPD PCR products obtained amplifying chromosomal DNA of studied <i>A</i>. <i>acidoterrestris</i> strains as the template, primer F-64 was used.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366920"], "description"=>"<p>pH growth profile of <i>A</i>. <i>acidoterrestris</i> strains (the error of pH was ±0.05).</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1581013, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.t001", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_pH_growth_profile_of_A_acidoterrestris_strains_the_error_of_pH_was_0_05_/1581013", "title"=>"pH growth profile of <i>A</i>. <i>acidoterrestris</i> strains (the error of pH was ±0.05).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366919"], "description"=>"<p>RAPD PCR products obtained amplifying chromosomal DNA of studied <i>A</i>. <i>acidoterrestris</i> strains as the template, primer F-61 was used.</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1581012, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g006", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RAPD_PCR_products_obtained_amplifying_chromosomal_DNA_of_studied_A_acidoterrestris_strains_as_the_template_primer_F_61_was_used_/1581012", "title"=>"RAPD PCR products obtained amplifying chromosomal DNA of studied <i>A</i>. <i>acidoterrestris</i> strains as the template, primer F-61 was used.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366901"], "description"=>"<p>Section <b>A</b>: Lanes 1 and 18: <i>A</i>. <i>acidoterrestris</i> DSM3922<sup>T</sup> and DSM2498. Lane 2: C1, lane 3: C2, lane 4: C3, lane 5: C4, lane 6; C5, lane 7: C6, lane 8: C8, lane 9: C10, lane 10: C11, lane 11: C12, lane 12: C13, lane 13: C14, lane 15: C15, lane 16: C17, lane 17: C18. Lanes M: size marker: O’GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific Inc. MA USA). Section <b>B</b>: Lanes 1 and 11: <i>A</i>. <i>acidoterrestris</i> DSM3922<sup>T</sup> and DSM2498. Lane 2: C19, lane 3: C20, lane 4: C21, lane 5: C22, lane 6; C23, lane 7: C24, lane 8: C25, lane 9: CB-1, lane 10: CB-2. Lanes M: size marker: O’GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific Inc. MA USA).</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1580994, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g004", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RAPD_PCR_products_obtained_amplifying_chromosomal_DNA_of_studied_A_acidoterrestris_strains_as_the_template_primer_BA_10_was_used_/1580994", "title"=>"RAPD PCR products obtained amplifying chromosomal DNA of studied <i>A</i>. <i>acidoterrestris</i> strains as the template, primer BA-10 was used.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366922"], "description"=>"<p>*Identification should be confirmed</p><p>The strain CB-1 was referred as “γ4” in Bevilacqua and Corbo [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141228#pone.0141228.ref005\" target=\"_blank\">5</a>].</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1581015, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.t003", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identity_percentage_between_16S_sequences_obtained_from_studied_strains_and_16S_sequences_filed_at_NCBI_website_/1581015", "title"=>"Identity percentage between 16S sequences obtained from studied strains and 16S sequences filed at NCBI website.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366921"], "description"=>"<p>Temperature profile of <i>A</i>. <i>acidoterrestris</i> strains (the error of T was ±1°C).</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1581014, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.t002", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Temperature_profile_of_A_acidoterrestris_strains_the_error_of_T_was_1_C_/1581014", "title"=>"Temperature profile of <i>A</i>. <i>acidoterrestris</i> strains (the error of T was ±1°C).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-10-20 04:17:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/2366891"], "description"=>"<p>ARG, deamination of arginine; LYS, decarboxylation of lysine; TRP, production of indole.</p>", "links"=>[], "tags"=>["fruit juices", "survival", "pattern", "RAPD PCR technique", "Species Characterization Alicyclobacillus acidoterrestris", "arginine", "spoilage", "strain", "lysine", "16 S rRNA", "soil species"], "article_id"=>1580991, "categories"=>["Uncategorised"], "users"=>["Antonio Bevilacqua", "Monica Mischitelli", "Valeria Pietropaolo", "Emanuela Ciuffreda", "Milena Sinigaglia", "Maria Rosaria Corbo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0141228.g002", "stats"=>{"downloads"=>3, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Eulero_Venn_diagram_for_amino_acid_utilization_/1580991", "title"=>"Eulero-Venn diagram for amino-acid utilization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-10-20 04:17:26"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"19", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"25", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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