OmpW of Caulobacter crescentus Functions as an Outer Membrane Channel for Cations
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{"title"=>"OmpW of caulobacter crescentus functions as an outer membrane channel for cations", "type"=>"journal", "authors"=>[{"first_name"=>"Roland", "last_name"=>"Benz", "scopus_author_id"=>"7103013456"}, {"first_name"=>"Michael D.", "last_name"=>"Jones", "scopus_author_id"=>"56438302400"}, {"first_name"=>"Farhan", "last_name"=>"Younas", "scopus_author_id"=>"57188238500"}, {"first_name"=>"Elke", "last_name"=>"Maier", "scopus_author_id"=>"7102488709"}, {"first_name"=>"Niraj", "last_name"=>"Modi", "scopus_author_id"=>"52164149700"}, {"first_name"=>"Reinhard", "last_name"=>"Mentele", "scopus_author_id"=>"35468906400"}, {"first_name"=>"Friedrich", "last_name"=>"Lottspeich", "scopus_author_id"=>"35432738300"}, {"first_name"=>"Ulrich", "last_name"=>"Kleinekathöfer", "scopus_author_id"=>"7004179274"}, {"first_name"=>"John", "last_name"=>"Smit", "scopus_author_id"=>"7202266091"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84961310597", "pui"=>"607865164", "doi"=>"10.1371/journal.pone.0143557", "isbn"=>"1932-6203", "sgr"=>"84961310597", "pmid"=>"26606672"}, "id"=>"417eaea0-accb-35ea-b716-28ef19fbd23f", "abstract"=>"Caulobacter crescentus is an oligotrophic bacterium that lives in dilute organic environments such as soil and freshwater. This bacterium represents an interesting model for cellular differentiation and regulation because daughter cells after division have different forms: one is motile while the other is non-motile and can adhere to surfaces. Interestingly, the known genome of C. crescentus does not contain genes predicted to code for outer membrane porins of the OmpF/C general diffusion type present in enteric bacteria or those coding for specific porins selective for classes of substrates. Instead, genes coding for 67 TonB-dependent outer membrane receptors have been identified, suggesting that active transport of specific nutrients may be the norm. Here, we report that high channel-forming activity was observed with crude outer membrane extracts of C. crescentus in lipid bilayer experiments, indicating that the outer membrane of C. crescentus contained an ion-permeable channel with a single-channel conductance of about 120 pS in 1M KCl. The channel-forming protein with an apparent molecular mass of about 20 kDa was purified to homogeneity. Partial protein sequencing of the protein indicated it was a member of the OmpW family of outer membrane proteins from Gram-negative bacteria. This channel was not observed in reconstitution experiments with crude outer membrane extracts of an OmpW deficient C. crescentus mutant. Biophysical analysis of the C. crescentus OmpW suggested that it has features that are special for general diffusion porins of Gram-negative outer membranes because it was not a wide aqueous channel. Furthermore, OmpW of C. crescentus seems to be different to known OmpW porins and has a preference for ions, in particular cations. A putative model for OmpW of C. crescentus was built on the basis of the known 3D-structures of OmpW of Escherichia coli and OprG of Pseudomonas aeruginosa using homology modeling. A comparison of the two known structures with the model of OmpW of C. crescentus suggested that it has a more hydrophilic interior and possibly a larger diameter.", "link"=>"http://www.mendeley.com/research/ompw-caulobacter-crescentus-functions-outer-membrane-channel-cations", "reader_count"=>15, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>8, "Agricultural and Biological Sciences"=>3, "Veterinary Science and Veterinary Medicine"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2578575"], "description"=>"<p>Lane 1: Molecular mass. Lane 2: OmpW solubilized at room temperature for 10 min in 5μl sample buffer. Lane 3: OmpW solubilized at 100°C for 10 min in 5 μl sample buffer.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612896, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g003", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Coomassie_stained_15_SDS_PAGE_of_OmpW_of_C_crescentus_obtained_by_elution_of_the_20_kDa_band_from_preparative_SDS_PAGE_/1612896", "title"=>"Coomassie stained, 15% SDS-PAGE of OmpW of <i>C</i>. <i>crescentus</i> obtained by elution of the 20 kDa band from preparative SDS-PAGE.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578578"], "description"=>"<p>A. Single-channel recordings of a PC/<i>n</i>-decane membrane in the presence of purified OmpW of <i>C</i>. <i>crescentus</i>. The aqueous phase contained 1 M KCl and 20 ng ml<sup>−1</sup> OmpW dissolved in 1% Genapol. The applied membrane potential was 20 mV; <i>T</i> = 20°C. B. Histogram of the probability <i>P</i>(<i>G</i>) for the occurrence of a given conductivity unit observed with membranes formed of PC/<i>n</i>-decane in the presence of OmpW of <i>C</i>. <i>crescentus</i>. <i>P</i>(<i>G</i>) is the probability that a given conductance increment <i>G</i> is observed in the single-channel experiments. It was calculated by dividing the number of fluctuations with a given conductance increment by the total number of conductance fluctuations. The aqueous phase contained 1 M KCl and about 20 ng/ml OmpW. The applied membrane potential was 20 mV; T = 20°C. A Gaussian function was applied to the histogram and the maximum of the curve was taken as average single-channel conductance. It was 117 (± 19) pS for 95 single-channel events.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612897, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g004", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Single_channel_recordings_of_a_PC_n_decane_membrane_in_the_presence_of_purified_OmpW_of_C_crescentus_The_aqueous_phase_contained_1_M_KCl_and_20_ng_ml_8722_1_OmpW_dissolved_in_1_Genapol_The_applied_membrane_potential_was_20_mV_T_20_C_B_Histogram_of_the_/1612897", "title"=>"A. Single-channel recordings of a PC/<i>n</i>-decane membrane in the presence of purified OmpW of <i>C</i>. <i>crescentus</i>. The aqueous phase contained 1 M KCl and 20 ng ml<sup>−1</sup> OmpW dissolved in 1% Genapol. The applied membrane potential was 20 mV; <i>T</i> = 20°C. B. Histogram of the probability <i>P</i>(<i>G</i>) for the occurrence of a given conductivity unit observed with membranes formed of PC/<i>n</i>-decane in the presence of OmpW of <i>C</i>. <i>crescentus</i>. <i>P</i>(<i>G</i>) is the probability that a given conductance increment <i>G</i> is observed in the single-channel experiments. It was calculated by dividing the number of fluctuations with a given conductance increment by the total number of conductance fluctuations. The aqueous phase contained 1 M KCl and about 20 ng/ml OmpW. The applied membrane potential was 20 mV; T = 20°C. A Gaussian function was applied to the histogram and the maximum of the curve was taken as average single-channel conductance. It w", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578588"], "description"=>"<p>Lane 1, <i>C</i>. <i>crescentus</i> PBS-EDTA extract. Lane 2, <i>C</i>. <i>crescentus</i> ΔompW PBS-EDTA extract. While PBS-EDTA extraction was a convenient method to enrich for OmpW, there was some variability between extractions, which can be seen in comparing protein profiles in lanes 1 and 2. Equal volume loadings were used. The star highlights the position of OmpW.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612902, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Coomassie_stained_12_SDS_PAGE_of_PBS_EDTA_extractions_from_wildtype_and_C_crescentus_ompW_/1612902", "title"=>"Coomassie stained, 12% SDS PAGE of PBS-EDTA extractions from wildtype and <i>C</i>. <i>crescentus</i> Δ<i>ompW</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578592"], "description"=>"<p>The aqueous phase contained 1 M KCl and 100 ng ml<sup>−1</sup> protein from enriched outer membranes treated with 0.5% LDAO. The applied membrane potential was 50 mV; <i>T</i> = 20°C. Note that the recording starts at the lower trace about 2 minutes after addition of the membrane extract and then continues in the upper trace.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612904, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Single_channel_recording_of_a_PC_n_decane_membrane_in_the_presence_of_enriched_outer_membranes_of_C_crescentus_ompW_mutant_/1612904", "title"=>"Single-channel recording of a PC/<i>n</i>-decane membrane in the presence of enriched outer membranes of <i>C</i>. <i>crescentus</i> ΔompW mutant.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578595"], "description"=>"<p>The data were taken from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.t001\" target=\"_blank\">Table 1</a>. The single-channel conductance of LamB of <i>E</i>. <i>coli</i> is given for comparison [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref045\" target=\"_blank\">45</a>].</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612905, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g007", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Single_channel_conductance_of_OmpW_in_1_M_salt_solution_as_a_function_of_the_ionic_radii_of_monovalent_cations_/1612905", "title"=>"Single-channel conductance of OmpW in 1 M salt solution as a function of the ionic radii of monovalent cations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578598"], "description"=>"<p>The alignment was performed using Pole Bioinformatique Lyonnaise Network Protein Sequence Analysis (<a href=\"http://npsa-pbil.ibcp.fr/\" target=\"_blank\">http://npsa-pbil.ibcp.fr</a>). Amino acids identical in all three proteins are highlighted in red, strongly similar amino acids (:) are given in green and weakly similar ones (.) in blue. The replacement of W155 of OmpW of <i>E</i>. <i>coli</i> and W170 of OprG of <i>P</i>. <i>aeruginosa</i> by K160 of <i>C</i>. <i>crescentus</i> is given in green color and is indicated by an arrow. The eight beta strands in OprG of <i>P</i>. <i>aeruginosa</i> and in OmpW of <i>E</i>. <i>coli</i> are numbered and indicated by blue bars [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref018\" target=\"_blank\">18</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref019\" target=\"_blank\">19</a>]. The yellow highlighted sequence was found by mass spectrometric analysis of tryptic peptides using Mascot N-terminal sequencing of OmpW of <i>C</i>. <i>crescentus</i> (<a href=\"http://www.matrixscience.com/\" target=\"_blank\">http://www.matrixscience.com/</a>).</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612906, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g008", "stats"=>{"downloads"=>4, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amino_acid_sequence_alignment_of_OprG_of_P_aeruginosa_OmpW_of_E_coli_and_OmpW_of_C_crescentus_/1612906", "title"=>"Amino acid sequence alignment of OprG of <i>P</i>. <i>aeruginosa</i>, OmpW of <i>E</i>. <i>coli</i> and OmpW of <i>C</i>. <i>crescentus</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578606"], "description"=>"<p>A. Comparison of structural features between a) OmpW <i>C</i>. <i>crescentus</i>, b) OmpW <i>E</i>. <i>coli</i> and c) OprG <i>P</i>. <i>aeruginosa</i>. Residues that are a part of a putative hydrophobic gate in <i>E</i>. <i>coli</i> (W155 and L56) and <i>P</i>. <i>aeruginosa</i> (W170 and V65) channels are shown as spheres. The corresponding residues in OmpW <i>C</i>. <i>crescentus</i> (K159 and I65) are also shown. Moreover, all channels are shown using a surface representation and color coded according to the residue type (Green: hydrophilic, white: hydrophobic, red: acidic, blue: basic). The channels are cut from the front to enable visualization of the channel surface interior. The black colored box indicates a putative ion transport pathway across the channel. EC and PC denote the extracellular and periplasmic sides of the channel, respectively. The models of the three OmpW homologs were modeled using the program Modeller [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref032\" target=\"_blank\">32</a>], taking the structure of <i>E</i>. <i>coli</i> OmpW as a template [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref018\" target=\"_blank\">18</a>]. B. Comparison of channel radii between <i>C</i>. <i>crescentus</i> OmpW (blue), E. coli OmpW (red) and OprG of <i>P</i>. <i>aeruginosa</i> (green) along the channel axis. The channel radii were calculated using the program HOLE [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref051\" target=\"_blank\">51</a>].</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612907, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g009", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Structural_properties_of_OmpW_of_C_crescentus_/1612907", "title"=>"Structural properties of OmpW of <i>C</i>. <i>crescentus</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578607"], "description"=>"<p>The membranes were formed from diphytanoyl phosphatidylcholine dissolved to 1% in <i>n</i>-decane. The aqueous solutions were used unbuffered and had a pH of 6 unless otherwise indicated. The applied voltage was 20 mV, and the temperature was 20°C. The average single-channel conductance, G (± SD), was calculated from at least 80 single events by averaging over all fluctuations or using a Gaussian distribution of the single-channel conductance (1 M KCl). The ionic radii of the ions were taken from literature sources [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref038\" target=\"_blank\">38</a>–<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref040\" target=\"_blank\">40</a>]. The radii of the hydrated cations were calculated from the limiting conductivities using the Stokes equation and were taken from [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref037\" target=\"_blank\">37</a>]. The data for the limiting conductivities of the different ions were taken from [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref041\" target=\"_blank\">41</a>]. The fact that the hydrated ion radius (Stokes radius) is in some cases smaller than the crystal radius is a phenomenon that is known from the literature (see ref. [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143557#pone.0143557.ref042\" target=\"_blank\">42</a>]).</p><p>Average single-channel conductance, G, of OmpW of <i>C</i>. <i>crescentus</i> in different salt solutions and radii, hydrated radii and limiting molar conductivity of the cations.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612908, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Average_single_channel_conductance_G_of_OmpW_of_C_crescentus_in_different_salt_solutions_and_radii_hydrated_radii_and_limiting_molar_conductivity_of_the_cations_/1612908", "title"=>"Average single-channel conductance, G, of OmpW of <i>C</i>. <i>crescentus</i> in different salt solutions and radii, hydrated radii and limiting molar conductivity of the cations.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578555"], "description"=>"<p>Lane 1, Crude membrane preparation. Lane 2, PBS-EDTA extract, not boiled prior to loading. Lane 3, PBS-EDTA extract, boiled prior to loading. The star highlights the position of OmpW.</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612887, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g001", "stats"=>{"downloads"=>2, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Coomassie_stained_12_SDS_PAGE_of_different_protein_preparations_of_C_crescentus_/1612887", "title"=>"Coomassie stained, 12% SDS-PAGE of different protein preparations of <i>C</i>. <i>crescentus</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2578559"], "description"=>"<p>A. Single-channel recordings of a PC/<i>n</i>-decane membrane in the presence of enriched outer membranes of <i>C</i>. <i>crescentus</i>. The aqueous phase contained 1 M KCl and 100 ng ml<sup>−1</sup> protein from enriched outer membranes treated with 0.5% LDAO. The applied membrane potential was 20 mV; <i>T</i> = 20°C. B. Histogram of the probability <i>P</i>(<i>G</i>) for the occurrence of a given conductivity unit observed with membranes formed of PC/<i>n</i>-decane in the presence of enriched outer membranes of <i>C</i>. <i>crescentus</i>. <i>P</i>(<i>G</i>) is the probability that a given conductance increment <i>G</i> is observed in the single-channel experiments. It was calculated by dividing the number of fluctuations with a given conductance increment by the total number of conductance fluctuations. The aqueous phase contained 1 M KCl. The applied membrane potential was 20 mV; T = 20°C. A Gaussian function was applied to the histogram and the maximum of the curve was taken as average single-channel conductance. It was 113 (± 22) pS for 105 single-channel events (left-hand maximum).</p>", "links"=>[], "tags"=>["membrane extracts", "Outer Membrane Channel", "ompw", "1 M KCl", "porin", "Partial protein sequencing", "lipid bilayer experiments", "Caulobacter crescentus Functions", "Cations Caulobacter crescentus"], "article_id"=>1612889, "categories"=>["Biological Sciences"], "users"=>["Roland Benz", "Michael D. Jones", "Farhan Younas", "Elke Maier", "Niraj Modi", "Reinhard Mentele", "Friedrich Lottspeich", "Ulrich Kleinekathöfer", "John Smit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0143557.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Single_channel_recordings_of_a_PC_n_decane_membrane_in_the_presence_of_enriched_outer_membranes_of_C_crescentus_The_aqueous_phase_contained_1_M_KCl_and_100_ng_ml_8722_1_protein_from_enriched_outer_membranes_treated_with_0_5_LDAO_The_applied_membrane_po/1612889", "title"=>"A. Single-channel recordings of a PC/<i>n</i>-decane membrane in the presence of enriched outer membranes of <i>C</i>. <i>crescentus</i>. The aqueous phase contained 1 M KCl and 100 ng ml<sup>−1</sup> protein from enriched outer membranes treated with 0.5% LDAO. The applied membrane potential was 20 mV; <i>T</i> = 20°C. B. Histogram of the probability <i>P</i>(<i>G</i>) for the occurrence of a given conductivity unit observed with membranes formed of PC/<i>n</i>-decane in the presence of enriched outer membranes of <i>C</i>. <i>crescentus</i>. <i>P</i>(<i>G</i>) is the probability that a given conductance increment <i>G</i> is observed in the single-channel experiments. It was calculated by dividing the number of fluctuations with a given conductance increment by the total number of conductance fluctuations. The aqueous phase contained 1 M KCl. The applied membrane potential was 20 mV; T = 20°C. A Gaussian function was applied to the histogram and the maximum of the curve was taken as", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-25 03:57:53"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
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  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
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  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
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  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
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  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"21", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"14", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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