A Multifunctional Frontloading Approach for Repeated Recycling of a Pressure-Controlled AFM Micropipette
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{"title"=>"A multifunctional frontloading approach for repeated recycling of a pressure-controlled AFM micropipette", "type"=>"journal", "authors"=>[{"first_name"=>"Phillip", "last_name"=>"Roder", "scopus_author_id"=>"56410770700"}, {"first_name"=>"Carsten", "last_name"=>"Hille", "scopus_author_id"=>"12647718400"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"607884520", "pmid"=>"26636981", "scopus"=>"2-s2.0-84955620843", "sgr"=>"84955620843", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0144157"}, "id"=>"db79e43c-48ea-382c-8def-beda25f767a1", "abstract"=>"Fluid force microscopy combines the positional accuracy and force sensitivity of an atomic force microscope (AFM) with nanofluidics via a microchanneled cantilever. However, adequate loading and cleaning procedures for such AFM micropipettes are required for various application situations. Here, a new frontloading procedure is described for an AFM micropipette functioning as a force- and pressure-controlled microscale liquid dispenser. This frontloading procedure seems especially attractive when using target substances featuring high costs or low available amounts. Here, the AFM micropipette could be filled from the tip side with liquid from a previously applied droplet with a volume of only a few μL using a short low-pressure pulse. The liquid-loaded AFM micropipettes could be then applied for experiments in air or liquid environments. AFM micropipette frontloading was evaluated with the well-known organic fluorescent dye rhodamine 6G and the AlexaFluor647-labeled antibody goat anti-rat IgG as an example of a larger biological compound. After micropipette usage, specific cleaning procedures were tested. Furthermore, a storage method is described, at which the AFM micropipettes could be stored for a few hours up to several days without drying out or clogging of the microchannel. In summary, the rapid, versatile and cost-efficient frontloading and cleaning procedure for the repeated usage of a single AFM micropipette is beneficial for various application situations from specific surface modifications through to local manipulation of living cells, and provides a simplified and faster handling for already known experiments with fluid force microscopy.", "link"=>"http://www.mendeley.com/research/multifunctional-frontloading-approach-repeated-recycling-pressurecontrolled-afm-micropipette", "reader_count"=>7, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Agricultural and Biological Sciences"=>1, "Chemistry"=>1, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}}, "reader_count_by_country"=>{"Switzerland"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4311604"], "description"=>"<p>(a-c) Bright-field images of four sequentially spotted droplets (pos. 1, 3 and 4: glycerol-water; pos. 2: 50 μM rhodamine 6G); micropipette silhouette can be seen in the background; scale bar = 50 μm. (d-f) Corresponding fluorescence images of the identical regions shown in (a-c) with associated intensity profile plots along the red lines; λ<sub>ex</sub> = 485 nm ± 15 nm, λ<sub>em</sub> = 535 nm ± 25 nm; time periods between the delivery of the individual droplets: 1 to 2 in 12 min, 1 to 3 in 39 min, 1 to 4 in 70 min.</p>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619170, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_AFM_micropipette_cleaning_procedure_when_using_the_organic_dye_rhodamine_6G_/1619170", "title"=>"AFM micropipette cleaning procedure when using the organic dye rhodamine 6G.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/4311610"], "description"=>"<p>(a) Bright-field image of four sequentially spotted droplets within 100 min (pos. 1 and 3: glycerol-water; pos. 2 and 4: 50 μM rhodamine 6G); the silhouette of the micropipette can be seen in the background; scale bar = 50 μm; time periods between the delivery of the individual droplets: 1 to 2 in 27 min, 1 to 3 in 74 min, 1 to 4 in 100 min. (b) Corresponding fluorescence image; λ<sub>ex</sub> = 485 nm ± 15 nm, λ<sub>em</sub> = 535 nm ± 25 nm. (c) Fluorescence intensity profile plot along the red line indicated in (b).</p>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619172, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alternate_spotting_of_water_and_rhodamine_6G_/1619172", "title"=>"Alternate spotting of water and rhodamine 6G.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/4311619"], "description"=>"<p>(a) Bright-field image of a spotted droplet of AlexaFluor647-labeled antibody IgG (10 μg/mL) and (b) the corresponding fluorescence image with an intensity profile plot; scale bar = 50 μm; λ<sub>ex</sub> = 640 nm ± 15 nm, λ<sub>em</sub> = 700 nm ± 37.5 nm. (c,d) Bright-field image and corresponding fluorescence image of the same spot as shown in (a,b, pos. 1), as well as the subsequently spotted glycerol-water droplet after the cleaning procedure (pos. 2); time period between the delivery of droplets 1 and 2 about 65 min. (e,f) Bright-field image and corresponding fluorescence image with an intensity plot of a spotted antibody droplet (pos. 3) due to repeated frontloading of the same micropipette after several hours of storage. (g,h) Corresponding images with an additional spotted glycerol-water droplet (pos. 4) after a repeated cleaning procedure; time period between the delivery of droplets 3 and 4 about 43 min.</p>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619175, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Repeated_AFM_micropipette_usage_for_antibody_spotting_/1619175", "title"=>"Repeated AFM micropipette usage for antibody spotting.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/4311565", "https://ndownloader.figshare.com/files/4311580", "https://ndownloader.figshare.com/files/4311589"], "description"=>"<div><p>Fluid force microscopy combines the positional accuracy and force sensitivity of an atomic force microscope (AFM) with nanofluidics <i>via</i> a microchanneled cantilever. However, adequate loading and cleaning procedures for such AFM micropipettes are required for various application situations. Here, a new frontloading procedure is described for an AFM micropipette functioning as a force- and pressure-controlled microscale liquid dispenser. This frontloading procedure seems especially attractive when using target substances featuring high costs or low available amounts. Here, the AFM micropipette could be filled from the tip side with liquid from a previously applied droplet with a volume of only a few μL using a short low-pressure pulse. The liquid-loaded AFM micropipettes could be then applied for experiments in air or liquid environments. AFM micropipette frontloading was evaluated with the well-known organic fluorescent dye rhodamine 6G and the AlexaFluor647-labeled antibody goat anti-rat IgG as an example of a larger biological compound. After micropipette usage, specific cleaning procedures were tested. Furthermore, a storage method is described, at which the AFM micropipettes could be stored for a few hours up to several days without drying out or clogging of the microchannel. In summary, the rapid, versatile and cost-efficient frontloading and cleaning procedure for the repeated usage of a single AFM micropipette is beneficial for various application situations from specific surface modifications through to local manipulation of living cells, and provides a simplified and faster handling for already known experiments with fluid force microscopy.</p></div>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619178, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.s001", "https://dx.doi.org/10.1371/journal.pone.0144157.s002", "https://dx.doi.org/10.1371/journal.pone.0144157.s003"], "stats"=>{"downloads"=>3, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Multifunctional_Frontloading_Approach_for_Repeated_Recycling_of_a_Pressure_Controlled_AFM_Micropipette_/1619178", "title"=>"A Multifunctional Frontloading Approach for Repeated Recycling of a Pressure-Controlled AFM Micropipette", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/4311592"], "description"=>"<p>Schematic illustration of (a) volume delivery on top of the probe holder by using a pipette, (b) loading position of the AFM scan head in the liquid above a chucked coverslip for frontloading (the lamellar liquid film reduces contamination of the entire clip), (c) zoom-in of (b) illustrating the frontloading process at the micropipette opening using low pressure (the black arrows indicate the running direction of the liquid), (d) dispensing of small liquid droplets onto a glass coverslip surface after successful frontloading procedure.</p>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619165, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representation_of_the_AFM_micropipette_frontloading_procedure_/1619165", "title"=>"Representation of the AFM micropipette frontloading procedure.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/4311598"], "description"=>"<p>Bright-field images of a micropipette microchannel (a) before and (b) after frontloading procedure resulting in a stable liquid level.</p>", "links"=>[], "tags"=>["application situations", "AFM micropipette", "AFM micropipette frontloading", "cleaning procedures", "dye rhodamine 6 G", "fluid force microscopy", "AFM micropipettes", "Multifunctional Frontloading Approach", "frontloading procedure"], "article_id"=>1619167, "categories"=>["Biophysics", "Physical Sciences not elsewhere classified", "Microbiology", "Physiology", "Biotechnology", "Chemical Sciences not elsewhere classified", "Plant Biology"], "users"=>["Phillip Roder", "Carsten Hille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144157.g002"], "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Visualization_of_the_AFM_micropipette_liquid_level_/1619167", "title"=>"Visualization of the AFM micropipette liquid level.", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-12-04 09:37:49"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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