A Safe and Stable Neonatal Vaccine Targeting GAPDH Confers Protection against Group B Streptococcus Infections in Adult Susceptible Mice
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A safe and stable neonatal vaccine targeting GAPDH confers protection against Group B Streptococcus infections in adult susceptible mice", "type"=>"journal", "authors"=>[{"first_name"=>"Joana", "last_name"=>"Alves", "scopus_author_id"=>"57197042735"}, {"first_name"=>"Pedro", "last_name"=>"Madureira", "scopus_author_id"=>"6506395469"}, {"first_name"=>"Maria Teresa", "last_name"=>"Baltazar", "scopus_author_id"=>"39260977700"}, {"first_name"=>"Leandro", "last_name"=>"Barros", "scopus_author_id"=>"56732589000"}, {"first_name"=>"Liliana", "last_name"=>"Oliveira", "scopus_author_id"=>"55953376800"}, {"first_name"=>"Ricardo Jorge", "last_name"=>"Dinis-Oliveira", "scopus_author_id"=>"14053913800"}, {"first_name"=>"Elva Bonifácio", "last_name"=>"Andrade", "scopus_author_id"=>"25640593500"}, {"first_name"=>"Adília", "last_name"=>"Ribeiro", "scopus_author_id"=>"56899194400"}, {"first_name"=>"Luís Mira", "last_name"=>"Vieira", "scopus_author_id"=>"7102882631"}, {"first_name"=>"Patrick", "last_name"=>"Trieu-Cuot", "scopus_author_id"=>"7004991750"}, {"first_name"=>"José Alberto", "last_name"=>"Duarte", "scopus_author_id"=>"35560897100"}, {"first_name"=>"Félix", "last_name"=>"Carvalho", "scopus_author_id"=>"7103070417"}, {"first_name"=>"Paula", "last_name"=>"Ferreira", "scopus_author_id"=>"35481294900"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0144196", "sgr"=>"84956604014", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"26673420", "issn"=>"19326203", "scopus"=>"2-s2.0-84956604014", "pui"=>"608001087"}, "id"=>"89ef2298-d71f-32fc-80c8-f2495cf8a8ee", "abstract"=>"Group B Streptococcus (GBS), a commensal organism, can turn into a life-threatening pathogen in neonates and elderly, or in adults with severe underlying diseases such as diabetes. We developed a vaccine targeting the GBS glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme detected at the bacterial surface, which was proven to be effective in a neonatal mouse model of infection. Since this bacterium has emerged as an important pathogen in non-pregnant adults, here we investigated whether this vaccine also confers protection in an adult susceptible and in a diabetic mouse model of infection. For immunoprotection studies, sham or immunized adult mice were infected with GBS serotype Ia and V strains, the two most prevalent serotypes isolated in adults. Sham and vaccinated mice were also rendered diabetic and infected with a serotype V GBS strain. For toxicological (pre-clinical) studies, adult mice were vaccinated three times, with three concentrations of recombinant GAPDH adjuvanted with Allydrogel, and the toxicity parameters were evaluated twenty-four hours after the last immunization. For the stability tests, the vaccine formulations were maintained at 4 degrees C for 6 and 12 months prior immunization. The results showed that all tested doses of the vaccine, including the stability study formulations, were immunogenic and that the vaccine was innocuous. The organs (brain, blood, heart, and liver) of vaccinated susceptible or diabetic adult mice were significantly less colonized compared to those of control mice. Altogether, these results demonstrate that the GAPDH-based vaccine is safe and stable and protects susceptible and diabetic adult mice against GBS infections. It is therefore a promising candidate as a global vaccine to prevent GBS-induced neonatal and adult diseases.", "link"=>"http://www.mendeley.com/research/safe-stable-neonatal-vaccine-targeting-gapdh-confers-protection-against-group-b-streptococcus-infect", "reader_count"=>13, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>2, "Professor"=>4, "Student > Doctoral Student"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>2, "Professor"=>4, "Student > Doctoral Student"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>4, "Neuroscience"=>1, "Computer Science"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Neuroscience"=>{"Neuroscience"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Spain"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84956604014"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84956604014?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84956604014&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84956604014&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84956604014", "dc:identifier"=>"SCOPUS_ID:84956604014", "eid"=>"2-s2.0-84956604014", "dc:title"=>"A safe and stable neonatal vaccine targeting GAPDH confers protection against Group B Streptococcus infections in adult susceptible mice", "dc:creator"=>"Alves J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-12-01", "prism:coverDisplayDate"=>"1 December 2015", "prism:doi"=>"10.1371/journal.pone.0144196", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal", "affiliation-city"=>"Porto", "affiliation-country"=>"Portugal"}, {"@_fa"=>"true", "affilname"=>"Universidade do Porto", "affiliation-city"=>"Porto", "affiliation-country"=>"Portugal"}, {"@_fa"=>"true", "affilname"=>"Instituto de Biologia Molecular e Celular, Porto", "affiliation-city"=>"Porto", "affiliation-country"=>"Portugal"}], "pubmed-id"=>"26673420", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0144196", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0144196", "share_count"=>1, "like_count"=>16, "comment_count"=>0, "click_count"=>0, "total_count"=>17}

Counter

  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"45", "xml_views"=>"11", "html_views"=>"445"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"115"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"4", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2609464"], "description"=>"<p>rGAPDH-specific IgG titers of 6 female Balb/c mice per group, immunized three times with a three week interval, 24h after the third immunization with 10 (V10), 20 (V20) or 40 (V40) μg of rGAPDH with 1:40 Alhydrogel, alhygrogel alone, or PBS. The ELISA plates were coated with rGAPDH and revealed with goat anti-mouse IgG—HRP. Titers are represented as minimal serum dilution necessary for the lost absorbance signal.</p>", "links"=>[], "tags"=>["Group B Streptococcus Infections", "serotype V GBS strain", "vaccine", "adult mice", "Stable Neonatal Vaccine Targeting GAPDH Confers Protection", "stability study formulations", "Mouse Model", "GBS serotype Ia", "Adult Susceptible Mice Group B Streptococcus"], "article_id"=>1625394, "categories"=>["Uncategorised"], "users"=>["Joana Alves", "Pedro Madureira", "Maria Teresa Baltazar", "Leandro Barros", "Liliana Oliveira", "Ricardo Jorge Dinis-Oliveira", "Elva Bonifácio Andrade", "Adília Ribeiro", "Luís Mira Vieira", "Patrick Trieu-Cuot", "José Alberto Duarte", "Félix Carvalho", "Paula Ferreira"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144196.g001", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_rGAPDH_vaccine_is_immunogenic_at_all_tested_doses_/1625394", "title"=>"rGAPDH vaccine is immunogenic at all tested doses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-12-18 11:20:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/2609466"], "description"=>"<p>rGAPDH-specific IgG titers of 4 female Balb/c mice per group, immunized three times with a three week interval, 24 h after the third immunization with PBS, Alhydrogel and 20 μg of rGAPDH in a 1:40 PBS/Alhydrogel suspension prepared fresh (S0) or conserved at 4°C for 6 (S6) and 12 (S12) months. The ELISA plates were coated with rGAPDH and revealed with goat anti-mouse IgG—HRP. Titers are represented as minimal serum dilution necessary for the lost absorbance signal. One-way ANOVA with post-hoc Tukey’s t-test. ns—not significant.</p>", "links"=>[], "tags"=>["Group B Streptococcus Infections", "serotype V GBS strain", "vaccine", "adult mice", "Stable Neonatal Vaccine Targeting GAPDH Confers Protection", "stability study formulations", "Mouse Model", "GBS serotype Ia", "Adult Susceptible Mice Group B Streptococcus"], "article_id"=>1625396, "categories"=>["Uncategorised"], "users"=>["Joana Alves", "Pedro Madureira", "Maria Teresa Baltazar", "Leandro Barros", "Liliana Oliveira", "Ricardo Jorge Dinis-Oliveira", "Elva Bonifácio Andrade", "Adília Ribeiro", "Luís Mira Vieira", "Patrick Trieu-Cuot", "José Alberto Duarte", "Félix Carvalho", "Paula Ferreira"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144196.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_rGAPDH_vaccine_preserves_its_potency_after_prolonged_storage_at_4_176_C_/1625396", "title"=>"rGAPDH vaccine preserves its potency after prolonged storage at 4°C.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-12-18 11:21:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/2609467"], "description"=>"<p>A) Kaplan–Meier survival curves. The lethality was monitored for 20 days. The numbers in parentheses represent the number of animals that survived out of the total number of infected animals. B) Blood, liver, lung, heart and brain colonization 6 and 18 hours post-infection (6 hours—Sham and GAPDH-immunized n = 7; 18 hours—Sham n = 7, rGAPDH-immunized n = 6) and C) sera, liver and spleen cytokine production 3 hours post-infection (n = 6 for both groups). Balb/c mice were immunized three times, with a three week interval, with 20 μg of rGAPDH in 1:40 Alhydrogel or treated with Alhydrogel alone and infected i.p. with 3x10<sup>6</sup> CFU of A909 (serotype Ia). Unpaired Student’s t-test. *p < 0.05; **p < 0.01; *** p < 0.001.</p>", "links"=>[], "tags"=>["Group B Streptococcus Infections", "serotype V GBS strain", "vaccine", "adult mice", "Stable Neonatal Vaccine Targeting GAPDH Confers Protection", "stability study formulations", "Mouse Model", "GBS serotype Ia", "Adult Susceptible Mice Group B Streptococcus"], "article_id"=>1625397, "categories"=>["Uncategorised"], "users"=>["Joana Alves", "Pedro Madureira", "Maria Teresa Baltazar", "Leandro Barros", "Liliana Oliveira", "Ricardo Jorge Dinis-Oliveira", "Elva Bonifácio Andrade", "Adília Ribeiro", "Luís Mira Vieira", "Patrick Trieu-Cuot", "José Alberto Duarte", "Félix Carvalho", "Paula Ferreira"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144196.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_rGAPDH_vaccination_improves_survival_and_induces_protection_against_serotype_Ia_GBS_infection_in_an_adult_mouse_model_/1625397", "title"=>"rGAPDH vaccination improves survival and induces protection against serotype Ia GBS infection in an adult mouse model.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-12-18 11:20:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/2609468"], "description"=>"<p>A) Kaplan–Meier survival curves. The lethality was monitored for 20 days. The numbers in parentheses represent the number of animals that survived out of the total number of infected animals. B) Blood. liver. lung. heart and brain colonization 6 and 18 hours post-infection and (6 hours—Sham n = 8. GAPDH-immunized n = 7; 18 hours—Sham n = 9. rGAPDH-immunized n = 8) C) sera. liver and spleen cytokine production 3 hours post-infection (n = 6 for both groups). Balb/c mice were immunized three times, with a three week interval, with 20 μg of rGAPDH in 1:40 Alhydrogel or treated with Alhydrogel alone and infected i.p. with 3x10<sup>7</sup> CFU of 2603V/R (serotype V). Unpaired Student’s t-test. *p < 0.05; **p < 0.01; *** p < 0.001.</p>", "links"=>[], "tags"=>["Group B Streptococcus Infections", "serotype V GBS strain", "vaccine", "adult mice", "Stable Neonatal Vaccine Targeting GAPDH Confers Protection", "stability study formulations", "Mouse Model", "GBS serotype Ia", "Adult Susceptible Mice Group B Streptococcus"], "article_id"=>1625398, "categories"=>["Uncategorised"], "users"=>["Joana Alves", "Pedro Madureira", "Maria Teresa Baltazar", "Leandro Barros", "Liliana Oliveira", "Ricardo Jorge Dinis-Oliveira", "Elva Bonifácio Andrade", "Adília Ribeiro", "Luís Mira Vieira", "Patrick Trieu-Cuot", "José Alberto Duarte", "Félix Carvalho", "Paula Ferreira"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144196.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_rGAPDH_vaccination_improves_survival_and_induces_protection_against_serotype_V_GBS_infection_in_an_adult_mouse_model_/1625398", "title"=>"rGAPDH vaccination improves survival and induces protection against serotype V GBS infection in an adult mouse model.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-12-18 11:20:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/2609469"], "description"=>"<p>Organ colonization of rGAPDH and Sham-immunized diabetic mice infected with GBS serotype V strain 2603V/R.</p>", "links"=>[], "tags"=>["Group B Streptococcus Infections", "serotype V GBS strain", "vaccine", "adult mice", "Stable Neonatal Vaccine Targeting GAPDH Confers Protection", "stability study formulations", "Mouse Model", "GBS serotype Ia", "Adult Susceptible Mice Group B Streptococcus"], "article_id"=>1625399, "categories"=>["Uncategorised"], "users"=>["Joana Alves", "Pedro Madureira", "Maria Teresa Baltazar", "Leandro Barros", "Liliana Oliveira", "Ricardo Jorge Dinis-Oliveira", "Elva Bonifácio Andrade", "Adília Ribeiro", "Luís Mira Vieira", "Patrick Trieu-Cuot", "José Alberto Duarte", "Félix Carvalho", "Paula Ferreira"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144196.t003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Organ_colonization_of_rGAPDH_and_Sham_immunized_diabetic_mice_infected_with_GBS_serotype_V_strain_2603V_R_/1625399", "title"=>"Organ colonization of rGAPDH and Sham-immunized diabetic mice infected with GBS serotype V strain 2603V/R.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-12-18 11:20:56"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"31", "full-text"=>"33", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"24", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts