Adsorption of Amorphous Silica Nanoparticles onto Hydroxyapatite Surfaces Differentially Alters Surfaces Properties and Adhesion of Human Osteoblast Cells
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{"title"=>"Adsorption of amorphous silica nanoparticles onto hydroxyapatite surfaces differentially alters surfaces properties and adhesion of human osteoblast cells", "type"=>"journal", "authors"=>[{"first_name"=>"Priya", "last_name"=>"Kalia", "scopus_author_id"=>"8582153700"}, {"first_name"=>"Roger A.", "last_name"=>"Brooks", "scopus_author_id"=>"7402358677"}, {"first_name"=>"Stephen D.", "last_name"=>"Kinrade", "scopus_author_id"=>"6604067181"}, {"first_name"=>"David J.", "last_name"=>"Morgan", "scopus_author_id"=>"55512389100"}, {"first_name"=>"Andrew P.", "last_name"=>"Brown", "scopus_author_id"=>"7408338729"}, {"first_name"=>"Neil", "last_name"=>"Rushton", "scopus_author_id"=>"7006323582"}, {"first_name"=>"Ravin", "last_name"=>"Jugdaohsingh", "scopus_author_id"=>"6603862985"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"26863624", "sgr"=>"84959432283", "doi"=>"10.1371/journal.pone.0144780", "scopus"=>"2-s2.0-84959432283", "pui"=>"608575216", "issn"=>"19326203"}, "id"=>"b89728c8-fbc8-3c17-951a-4b92146b94fd", "abstract"=>"Silicon (Si) is suggested to be an important/essential nutrient for bone and connective tissue health. Silicon-substituted hydroxyapatite (Si-HA) has silicate ions incorporated into its lattice structure and was developed to improve attachment to bone and increase new bone formation. Here we investigated the direct adsorption of silicate species onto an HA coated surface as a cost effective method of incorporating silicon on to HA surfaces for improved implant osseointegration, and determined changes in surface characteristics and osteoblast cell adhesion. Plasma-sprayed HA-coated stainless steel discs were incubated in silica dispersions of different concentrations (0?42 mM Si), at neutral pH for 12 h. Adsorbed Si was confirmed by XPS analysis and quantified by ICP-OES analysis following release from the HA surface. Changes in surface characteristics were determined by AFM and measurement of surface wettability. Osteoblast cell adhesion was determined by vinculin plaque staining. Maximum Si adsorption to the HA coated disc occurred after incubation in the 6 mM silica dispersion and decreased progressively with higher silica concentrations, while no adsorption was observed with dispersions below 6 mM Si. Comparison of the Si dispersions that produced the highest and lowest Si adsorption to the HA surface, by TEM-based analysis, revealed an abundance of small amorphous nanosilica species (NSP) of ~1.5 nm in diameter in the 6 mM Si dispersion, with much fewer and larger NSP in the 42 mM Si dispersions. 29Si-NMR confirmed that the NSPs in the 6 mM silica dispersion were polymeric and similar in composition to the larger NSPs in the 42 mM Si dispersion, suggesting that the latter were aggregates of the former. Amorphous NSP adsorbed from the 6 mM dispersion on to a HA-coated disc surface increased the surface?s water contact angle by 53�, whereas that adsorbed from the 42 mM dispersion decreased the contact angle by 18�, indicating increased and decreased hydrophobicity, respectively. AFM showed an increase in surface roughness of the 6 mM Si treated surface, which correlated well with an increase in number of vinculin plaques. These findings suggest that NSP of the right size (relative to charge) adsorb readily to the HA surface, changing the surface characteristics and, thus, improving osteoblast cell adhesion. This treatment provides a simple way to modify plasma-coated HA surfaces that may enable improved osseointegration of bone implants.", "link"=>"http://www.mendeley.com/research/adsorption-amorphous-silica-nanoparticles-onto-hydroxyapatite-surfaces-differentially-alters-surface", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Materials Science"=>3, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Chemical Engineering"=>2, "Chemistry"=>1, "Earth and Planetary Sciences"=>2}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Unspecified"=>{"Unspecified"=>3}, "Chemical Engineering"=>{"Chemical Engineering"=>2}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2636943"], "description"=>"<p>Release of Si from silica-treated HA-coated stainless steel discs as a function of time: A) into SBF at pH 7.2 and 25°C; B) into McCoy’s 5A supplemented medium at pH 7.4, 37°C and 5% CO<sub>2</sub>; and C) in McCoy’s 5A medium at pH 7.4, 37°C and 5% CO<sub>2</sub> in the presence of primary human osteoblasts (inset shows an increased y-scale). Data are means ± SD of <i>n</i> = 3 discs per time point, and were corrected for adsorbed Si from the control (0 mM Si) solution.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644192, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_/1644192", "title"=>"Adsorption of Amorphous Silica Nanoparticles onto Hydroxyapatite Surfaces Differentially Alters Surfaces Properties and Adhesion of Human Osteoblast Cells", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636945"], "description"=>"<p>Plaques were sorted A) by shape (long, short or punctuate) and B) by cell location (peripheral, nuclear or cytoplasmic). Significant differences (<i>P</i> ≤ 0.05) between silica treatment and control (0 mM Si) are represented by an asterisk.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644194, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g007", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Number_of_vinculin_plaques_per_human_osteoblast_cell_after_48_h_growth_on_HA_coated_discs_treated_with_0_6_or_42_mM_silica_dispersion_/1644194", "title"=>"Number of vinculin plaques per human osteoblast cell after 48 h growth on HA-coated discs treated with 0, 6 or 42 mM silica dispersion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636948", "https://ndownloader.figshare.com/files/2636949", "https://ndownloader.figshare.com/files/2636950", "https://ndownloader.figshare.com/files/2636951"], "description"=>"<div><p>Silicon (Si) is suggested to be an important/essential nutrient for bone and connective tissue health. Silicon-substituted hydroxyapatite (Si-HA) has silicate ions incorporated into its lattice structure and was developed to improve attachment to bone and increase new bone formation. Here we investigated the direct <i>adsorption</i> of silicate species onto an HA coated surface as a cost effective method of incorporating silicon on to HA surfaces for improved implant osseointegration, and determined changes in surface characteristics and osteoblast cell adhesion. Plasma-sprayed HA-coated stainless steel discs were incubated in silica dispersions of different concentrations (0–42 mM Si), at neutral pH for 12 h. Adsorbed Si was confirmed by XPS analysis and quantified by ICP-OES analysis following release from the HA surface. Changes in surface characteristics were determined by AFM and measurement of surface wettability. Osteoblast cell adhesion was determined by vinculin plaque staining. Maximum Si adsorption to the HA coated disc occurred after incubation in the 6 mM silica dispersion and decreased progressively with higher silica concentrations, while no adsorption was observed with dispersions below 6 mM Si. Comparison of the Si dispersions that produced the highest and lowest Si adsorption to the HA surface, by TEM-based analysis, revealed an abundance of small amorphous nanosilica species (NSP) of ~1.5 nm in diameter in the 6 mM Si dispersion, with much fewer and larger NSP in the 42 mM Si dispersions. <sup>29</sup>Si-NMR confirmed that the NSPs in the 6 mM silica dispersion were polymeric and similar in composition to the larger NSPs in the 42 mM Si dispersion, suggesting that the latter were aggregates of the former. Amorphous NSP adsorbed from the 6 mM dispersion on to a HA-coated disc surface increased the surface’s water contact angle by 53°, whereas that adsorbed from the 42 mM dispersion decreased the contact angle by 18°, indicating increased and decreased hydrophobicity, respectively. AFM showed an increase in surface roughness of the 6 mM Si treated surface, which correlated well with an increase in number of vinculin plaques. These findings suggest that NSP of the right size (relative to charge) adsorb readily to the HA surface, changing the surface characteristics and, thus, improving osteoblast cell adhesion. This treatment provides a simple way to modify plasma-coated HA surfaces that may enable improved osseointegration of bone implants.</p></div>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644197, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0144780.s001", "https://dx.doi.org/10.1371/journal.pone.0144780.s002", "https://dx.doi.org/10.1371/journal.pone.0144780.s003", "https://dx.doi.org/10.1371/journal.pone.0144780.s004"], "stats"=>{"downloads"=>4, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Adsorption_of_Amorphous_Silica_Nanoparticles_onto_Hydroxyapatite_Surfaces_Differentially_Alters_Surfaces_Properties_and_Adhesion_of_Human_Osteoblast_Cells_/1644197", "title"=>"Adsorption of Amorphous Silica Nanoparticles onto Hydroxyapatite Surfaces Differentially Alters Surfaces Properties and Adhesion of Human Osteoblast Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636929"], "description"=>"<p>In this sample, crystallinity of the HA was 99.6% following plasma-spraying. The brown line shows the experimental XRD scan of the sample and the vertical blue lines indicate the match with Joint Committee on Powder Diffraction Standards reference HA. Standard tricalcium phosphate (red) and calcium oxide phosphate (green) are also shown.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644178, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_XRD_analysis_showing_phase_purity_of_HA_coating_after_plasma_spraying_on_to_stainless_steel_discs_/1644178", "title"=>"XRD analysis showing phase purity of HA coating after plasma-spraying on to stainless steel discs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636931"], "description"=>"<p><u>Q</u><sup><i>n</i></sup> represents a Si atom with <i>n</i> coordinated -OSi groups.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644180, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_29_Si_NMR_spectra_99_35_MHz_at_25_176_C_of_a_6_and_b_42_mM_silica_dispersions_/1644180", "title"=>"<sup>29</sup>Si NMR spectra (99.35 MHz) at 25°C of a) 6 and b) 42 mM silica dispersions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636935"], "description"=>"<p>(A) Percentage of total silica in 6 and 42 mM silica dispersions that passed through 10, 50 100 and 1000 kDa MWCO (molecular weight cut-off) membranes. (B) TEM analysis of the filtrates. i) TEM of amorphous nanoscale silica particles (NSP) in the filtrate of the 10 kDa MWCO filtered 6 mM silica dispersion; ii) TEM showing larger (< 20 nm) NSP in the filtrate of the filtered 42 mM silica dispersion; iii) Bright field, zero loss filtered TEM image of NSP in the filtrate of the filtered 42 mM dispersion and iv) EF-TEM Si <i>L</i><sub>2,3</sub> elemental map image of NSP shown in (iii). The bright regions indicate Si-rich areas.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644184, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g003", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_/1644184", "title"=>"Adsorption of Amorphous Silica Nanoparticles onto Hydroxyapatite Surfaces Differentially Alters Surfaces Properties and Adhesion of Human Osteoblast Cells", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636937"], "description"=>"<p>Data were corrected for adsorbed Si from the control (0 mM Si) solution. Data are means ± SD of <i>n</i> = 4 discs per treatment.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644186, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Silicon_adsorbed_onto_HA_coated_stainless_steel_discs_following_incubation_in_silica_dispersions_containing_A_1_to_42_mM_Si_and_B_6_to_12_mM_Si_/1644186", "title"=>"Silicon adsorbed onto HA-coated stainless steel discs following incubation in silica dispersions containing (A) 1 to 42 mM Si and (B) 6 to 12 mM Si.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/2636941"], "description"=>"<p>Actual roughness measurements are presented in <b><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144780#pone.0144780.t002\" target=\"_blank\">Table 2</a></b>.</p>", "links"=>[], "tags"=>["Osteoblast cell adhesion", "HA surface", "6 mM dispersion", "nsp", "xps", "42 mM Si dispersion", "Human Osteoblast Cells Silicon", "42 mM Si dispersions", "afm", "42 mM dispersion", "6 mM Si dispersion", "6 mM silica dispersion", "Hydroxyapatite Surfaces Differentially Alters Surfaces Properties", "surface characteristics", "vinculin plaque staining", "Amorphous Silica Nanoparticles", "Maximum Si adsorption", "6 mM Si"], "article_id"=>1644190, "categories"=>["Biological Sciences"], "users"=>["Priya Kalia", "Roger A. Brooks", "Stephen D. Kinrade", "David J. Morgan", "Andrew P. Brown", "Neil Rushton", "Ravin Jugdaohsingh"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0144780.g005", "stats"=>{"downloads"=>2, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_images_of_atomic_force_microscopy_AFM_carried_on_out_plasma_sprayed_HA_surfaces_following_incubation_in_0_A_B_6_C_D_and_42_mM_E_F_Si_dispersions_for_12_h_and_air_dried_/1644190", "title"=>"Representative images of atomic force microscopy (AFM) carried on out plasma-sprayed HA surfaces following incubation in 0 (A, B), 6 (C, D) and 42 mM (E, F) Si dispersions for 12 h and air-dried.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-10 11:26:14"}

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{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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