Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells
Publication Date
January 11, 2016
Journal
PLOS ONE
Authors
Fernando J. Reyes Zurita, Eva E. Rufino Palomares, Leticia García Salguero, Juan Peragón, et al
Volume
11
Issue
1
Pages
e0146178
DOI
https://dx.plos.org/10.1371/journal.pone.0146178
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0146178
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26751572
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709006
Europe PMC
http://europepmc.org/abstract/MED/26751572
Web of Science
000367888100050
Scopus
84954448499
Mendeley
http://www.mendeley.com/research/maslinic-acid-natural-triterpene-induces-death-receptormediated-apoptotic-mechanism-caco2-p53deficie
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2624764"], "description"=>"<p>Caco-2 and HT29 cells were treated with MA at IC<sub>50</sub> and IC<sub>80</sub> concentrations for 4 h. The levels of protein expression are expressed as arbitrary intensity units of each band compared to arbitrary intensity units of actin. MA produced clear effects on this protein in Caco-2 cells. However, this effect was not observable in HT29 cells. The values represent means ± SD. of at least three independent experiments performed in triplicate. Key: <i>(*) p<0</i>.<i>05</i> and <i>(**) p<0</i>.<i>01</i>, with respect to the untreated cells.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635698, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g005", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blotting_to_determine_Bid_and_t_Bid_levels_in_Caco_2_cells_Top_and_HT29_cells_Bottom_/1635698", "title"=>"Western blotting to determine Bid and t-Bid levels in Caco-2 cells (Top) and HT29 cells (Bottom).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-18 15:13:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624767"], "description"=>"<p>MA is able to activate both intrinsic and extrinsic apoptotic mechanisms according to the type of cell involved. <i>Abbreviations</i>: Cps, caspase; cit c, cytochrome-c.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635701, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g006", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_representation_of_the_different_mechanisms_proposed_for_the_induction_of_apoptosis_by_MA_in_colon_cancer_Caco_2_cells_right_and_HT29_cells_left_/1635701", "title"=>"Schematic representation of the different mechanisms proposed for the induction of apoptosis by MA in colon-cancer Caco-2 cells (right) and HT29 cells (left).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-18 15:13:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624768"], "description"=>"<div><p>Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.</p></div>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635702, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178", "stats"=>{"downloads"=>4, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells_/1635702", "title"=>"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2016-01-18 15:13:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624752"], "description"=>"<p><b>(Panel A)</b> Structure of the natural pentacyclic triterpene, maslinic acid [(2α, 3β)-2,3-dihydroxyolean-12-en-28-oic acid]. <b>(Panel B)</b> Inhibitory effect of maslinic acid on the viability of Caco-2 cells. IC<sub>50</sub> is the concentration of MA required for 50% growth inhibition. Each point represents the mean value ± S.D. of at least three independent experiments performed in triplicate. <b>(Panel C)</b>Top: histograms of Caco-2 cell cycle after 72 h treatment with MA. Bottom: percentage of cells in each of the cell-cycle phases. Caco-2 cells were untreated (first column) or treated with MA at IC<sub>50</sub> (second column) or IC<sub>80</sub> (third column) concentrations. Cell-cycle analysis was conducted after propidium iodide staining. Values represent means ± S.D. of at least three independent experiments performed in triplicate. Key: <i>(*) p<0</i>.<i>05</i> and <i>(**) p<0</i>.<i>01</i>, respect to the untreated control cells.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635690, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g001", "stats"=>{"downloads"=>1, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_/1635690", "title"=>"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-19 08:10:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624758"], "description"=>"<p>Caco-2 cells were treated with MA at IC<sub>50</sub> and IC<sub>80</sub> concentrations for 4 h. The levels of protein expression are expressed as arbitrary intensity units of each band compared to arbitrary intensity units of actin. The values represent means ± S.D. of at least three independent experiments performed in triplicate. Key: <i>(*) p<0</i>.<i>05</i>, <i>(**) p<0</i>.<i>01</i> and <i>(***) p<0</i>.<i>001</i>, with respect to the untreated control cells.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635692, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Top_western_blotting_of_the_levels_of_pro_caspase_9_and_caspase_9_centre_pro_caspase_8_and_caspase_8_and_bottom_pro_caspase_3_and_caspase_3_proteins_/1635692", "title"=>"Top: western blotting of the levels of pro-caspase-9 and caspase-9; centre: pro-caspase-8 and caspase-8; and bottom: pro-caspase-3 and caspase-3 proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-18 15:13:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624759"], "description"=>"<p><b>(Panel A)</b> Maslinic acid induced apoptosis through activation of caspase-8 in Caco-2 cells (Top), but not in HT29 cells (Bottom). Caco-2 and HT29 cells were treated for 4 h with their corresponding IC<sub>50</sub> or IC<sub>80</sub> concentrations. Values represent means ± S.D. of four experiments performed in triplicate. Key <i>(***) P<0</i>.<i>001</i>, with respect to the untreated control cells. <b>(Panel B)</b> Diagrams of rhodamine 123/propidium iodide flow-cytometry. The right quadrants of each diagram (Q2 and Q4) represent positive cells stained with Rh123, the left quadrants (Q1 and Q3) represent negative cells stained with Rh123. Top-right, percentage of Rh123 positive cell population in control cells (CT), IC<sub>50</sub> and IC<sub>80</sub> concentrations, after 4 h of treatment. The values represent means ± SD. of four independent experiments performed in triplicate. Not significant differences were found.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635693, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g003", "stats"=>{"downloads"=>0, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_/1635693", "title"=>"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-19 08:10:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2624761"], "description"=>"<p><b>(Panel A)</b> Western blotting to determine Bax levels. Caco-2 cells were treated with MA at IC<sub>50</sub> and IC<sub>80</sub> concentrations for 4 h. <b>(Panel B)</b> Western blotting to determine cytosolic <i>cytochrome-c</i> release levels in Caco-2 cells (Top) and HT29 cells (Bottom). Caco-2 and HT29 cells were treated with MA at IC<sub>50</sub> and IC<sub>80</sub> concentrations for 4h. The levels of protein expression are expressed as arbitrary intensity units of each band compared to arbitrary intensity units of actin. The values represent means ± SD. of at least three independent experiments performed in triplicate. Key: <i>(*) p<0</i>.<i>05</i>, respect to the untreated cells.</p>", "links"=>[], "tags"=>["cell type", "apoptotic pathways", "extrinsic apoptotic pathway", "culture medium", "apoptotic mechanism", "caco", "Bax protein", "Natural Triterpene", "apoptotic cell death", "mitochondrial apoptotic pathway", "HT 29 colon cancer cells", "ma", "p 53. Natural antitumoral agents", "Mitochondrial membrane"], "article_id"=>1635695, "categories"=>["Uncategorised"], "users"=>["Fernando J. Reyes-Zurita", "Eva E. Rufino-Palomares", "Leticia García-Salguero", "Juan Peragón", "Pedro P. Medina", "Andrés Parra", "Marta Cascante", "José A. Lupiáñez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0146178.g004", "stats"=>{"downloads"=>1, "page_views"=>47, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_/1635695", "title"=>"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-01-19 08:10:32"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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