Odontogenic Differentiation of Human Dental Pulp Stem Cells on Hydrogel Scaffolds Derived from Decellularized Bone Extracellular Matrix and Collagen Type I
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{"title"=>"Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I", "type"=>"journal", "authors"=>[{"first_name"=>"Francesco", "last_name"=>"Paduano", "scopus_author_id"=>"56958346400"}, {"first_name"=>"Massimo", "last_name"=>"Marrelli", "scopus_author_id"=>"34868383000"}, {"first_name"=>"Lisa J.", "last_name"=>"White", "scopus_author_id"=>"35276790600"}, {"first_name"=>"Kevin M.", "last_name"=>"Shakesheff", "scopus_author_id"=>"7004564373"}, {"first_name"=>"Marco", "last_name"=>"Tatullo", "scopus_author_id"=>"34868807900"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84973356009", "pmid"=>"26882351", "pui"=>"610824699", "isbn"=>"1932-6203", "scopus"=>"2-s2.0-84973356009", "doi"=>"10.1371/journal.pone.0148225", "issn"=>"19326203"}, "id"=>"58c0ff83-9e2a-3d4e-b57f-3b4ceacfc7ae", "abstract"=>"OBJECTIVES: The aim of this study was to evaluate the level of odontogenic differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM. METHODS: DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and mineral deposition was observed by Von Kossa staining. RESULTS: When DPSCs were cultured on bECM hydrogels, the mRNA expression levels of DSPP, DMP-1 and MEPE genes were significantly upregulated with respect to those cultured on Col-I scaffolds or TCPS in the absence of extra odontogenic inducers. In addition, more mineral deposition was observed on bECM hydrogel scaffolds as demonstrated by Von Kossa staining. Moreover, DSPP, DMP-1 and MEPE mRNA expressions of DPSCs cultured on bECM hydrogels were further upregulated by the addition of GFs or osteo/odontogenic medium compared to Col-I treated cells in the same culture conditions. SIGNIFICANCE: These results demonstrate the potential of the bECM hydrogel scaffolds to stimulate odontogenic differentiation of DPSCs.", "link"=>"http://www.mendeley.com/research/odontogenic-differentiation-human-dental-pulp-stem-cells-hydrogel-scaffolds-derived-decellularized-b", "reader_count"=>45, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>3, "Other"=>3, "Student > Master"=>7, "Student > Bachelor"=>4, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>3, "Other"=>3, "Student > Master"=>7, "Student > Bachelor"=>4, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>10, "Unspecified"=>2, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>2, "Medicine and Dentistry"=>14, "Agricultural and Biological Sciences"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>4, "Physics and Astronomy"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>10}, "Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>14}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>4}}, "reader_count_by_country"=>{"India"=>1}, "group_count"=>3}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/3980230"], "description"=>"<p>(A) Representative images of human DPSCs cultured on bECM hydrogel scaffolds (3, 4, 6, 8 mg/ml) or Col-I hydrogel scaffolds (3, 4, 6, 8 mg/ml) in basal medium for 3 weeks, scale bar: 100 μm. (B) qRT-PCR at 3 weeks for odontogenic markers DSPP, DMP-1 and MEPE of DPSCs seeded on bECM (3, 4, 6, 8 mg/ml), Col-I (3, 4, 6, 8 mg/ml) hydrogel scaffolds or TCPS cultured in basal medium compared to those seeded on TCPS in presence of osteo/odontogenic medium (OM). The results are presented as the fold increase (2<sup>-(ΔΔCT)</sup>) with respect to the level expressed in DPSCs seeded on TCPS cultured in basal medium. All values are expressed as the means ± SDs and were normalised to HPRT expression levels, comparison by unpaired two-tailed Student’s t-test. *<i>P</i> < 0.05, **<i>P <</i> 0.01. (C) Von Kossa staining of human DPSCs cultured on bECM (3, 4, 6, 8 mg/ml) and Col-I (3, 4, 6, 8 mg/ml) hydrogel scaffolds in basal medium for 3 weeks. Black color indicates mineral deposition. Scale bar: 100 μm.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342416, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.g003", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Cell_morphology_mineralization_and_relative_mRNA_expression_of_odontogenic_genes_in_DPSCs_cultured_on_bECM_or_Col_I_hydrogel_scaffolds_in_normal_culture_conditions_/2342416", "title"=>"Cell morphology, mineralization and relative mRNA expression of odontogenic genes in DPSCs cultured on bECM or Col-I hydrogel scaffolds in normal culture conditions.", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980197"], "description"=>"<p>(A) Representative images of DPSCs seeded on bECM scaffolds (3, 4, 6, 8 mg/ml) at day 14 and 21. DPSCs proliferate, elongate and form clusters, scale bar: 100 μm. (B) DPSCs remained viable after 14 and 21 days of culture on 3, 4, 6, 8 mg/ml bECM hydrogel scaffolds. Viable cells stained green and dead cells stained red after CMFDA/PI staining. Cells were observed under fluorescence confocal microscope, scale bar: 100 μm.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342386, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.g002", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Cell_morphology_and_viability_of_DPSCs_in_bECM_scaffolds_i_in_vitro_i_/2342386", "title"=>"Cell morphology and viability of DPSCs in bECM scaffolds <i>in vitro</i>.", "pos_in_sequence"=>3, "defined_type"=>1, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980287"], "description"=>"<p>(A) Representative images of human DPSCs cultured on 4 mg/ml bECM or 4 mg/ml Col-I hydrogel scaffolds in osteo/odontogenic medium (OM) for 3 weeks, scale bar: 100 μm. (B) qRT-PCR at 3 weeks for odontogenic markers DSPP, DMP-1 and MEPE of DPSCs seeded on 4 mg/ml bECM hydrogel scaffold, 4 mg/ml Col-I or TCPS cultured in osteo/odontogenic medium. The results are presented as the fold increase (2<sup>-(ΔΔCT)</sup>) with respect to the level expressed in DPSCs seeded on TCPS cultured in osteo/odontogenic medium. The data shown are the means ± SDs of three independent experiments, *<i>P</i> < 0.05, ** <i>P</i> < 0.01 compared with DPSCs cultured osteo/odontogenic medium or 4 mg/ml bECM cultured in basal medium. OM: osteo/odontogenic medium.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342467, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.g004", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Morphology_and_odontogenic_gene_expression_of_DPSCs_on_bECM_or_Col_I_hydrogel_scaffolds_cultured_in_medium_containing_odontogenic_inducers_/2342467", "title"=>"Morphology and odontogenic gene expression of DPSCs on bECM or Col-I hydrogel scaffolds cultured in medium containing odontogenic inducers.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980155"], "description"=>"<p>(A) Immunophenotype analysis of CD13, CD29, CD44, CD73, CD90, CD105, CD146, CD45 and HLA-DR expression in DPSCs by flow cytometry. The green histograms represent the cell count for the specific antibody, and the blue histograms represent the fluorescence of the negative control. The percentage of cells positive for each antigen is shown in the figure. (B) Phase contrast and Von Kossa staining of human DPSCs cultured on TCPS and maintained in basal or osteo/odontogenic medium for 3 weeks (dark color indicates mineral nodules), scale bar: 100 μm. OM: osteo/odontogenic medium. (C) qRT-PCR at 3 weeks for odontogenic markers DSPP, DMP-1 and MEPE of DPSCs seeded on TCPS cultured in basal medium compared to those cultured in osteo/odontogenic medium. The results are presented as the fold increase (2<sup>-(ΔΔCT)</sup>) with respect to the level expressed in DPSCs cultured in basal medium. All values are expressed as the means ± SDs and were normalised to HPRT expression levels, comparison by unpaired two-tailed Student’s t-test. *<i>P</i> < 0.05. OM: osteo/odontogenic medium.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342341, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.g001", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/MSCs_surface_markers_and_odontogenic_genes_expression_of_DPSCs_cultured_on_TCPS_tissue_culture_polystyrene_prior_to_seeding_into_bECM_hydrogel_scaffolds_/2342341", "title"=>"MSCs surface markers and odontogenic genes expression of DPSCs cultured on TCPS (tissue culture polystyrene) prior to seeding into bECM hydrogel scaffolds.", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980413"], "description"=>"<p>Primer sequences used in qRT-PCR analysis.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342566, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.t001", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Primer_sequences_used_in_qRT_PCR_analysis_/2342566", "title"=>"Primer sequences used in qRT-PCR analysis.", "pos_in_sequence"=>7, "defined_type"=>3, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980353"], "description"=>"<p>(A) Representative images of human DPSCs cultured on bECM scaffolds (4 mg/ml) and Col-I (4 mg/ml) in medium containing GFs (FGFb + EGF) for 3 weeks, scale bar: 100 μm. (B) qRT-PCR at 3 weeks for odontogenic markers DSPP, DMP-1 and MEPE of DPSCs seeded on 4 mg/ml bECM or 4 mg/ml Col-I hydrogels cultured in medium containing GFs (FGFb + EGF) compared to those seeded on TCPS and cultured in basal or osteo/odontogenic medium (OM). The results are presented as the fold increase (2<sup>-(ΔΔCT)</sup>) with respect to the level expressed in DPSCs seeded on TCPS cultured in basal medium. The data shown are the means ± SDs of three independent experiments, *<i>P</i> < 0.05, ** <i>P</i> < 0.01 compared with TCPS-cultured cells in basal medium, osteo/odontogenic medium or seeded on 4 mg/ml bECM cultured in basal medium. OM: osteo/odontogenic medium, GFs: FGFb + EGF.</p>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342521, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148225.g005", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Distribution_and_odontogenic_gene_expression_of_DPSCs_on_bECM_or_Col_I_scaffolds_cultured_in_medium_containing_growth_factors_GFs_/2342521", "title"=>"Distribution and odontogenic gene expression of DPSCs on bECM or Col-I scaffolds cultured in medium containing growth factors (GFs).", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-02-16 07:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/3980053", "https://ndownloader.figshare.com/files/3980062", "https://ndownloader.figshare.com/files/3980086"], "description"=>"<div><p>Objectives</p><p>The aim of this study was to evaluate the level of odontogenic differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM.</p><p>Methods</p><p>DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and mineral deposition was observed by Von Kossa staining.</p><p>Results</p><p>When DPSCs were cultured on bECM hydrogels, the mRNA expression levels of DSPP, DMP-1 and MEPE genes were significantly upregulated with respect to those cultured on Col-I scaffolds or TCPS in the absence of extra odontogenic inducers. In addition, more mineral deposition was observed on bECM hydrogel scaffolds as demonstrated by Von Kossa staining. Moreover, DSPP, DMP-1 and MEPE mRNA expressions of DPSCs cultured on bECM hydrogels were further upregulated by the addition of GFs or osteo/odontogenic medium compared to Col-I treated cells in the same culture conditions.</p><p>Significance</p><p>These results demonstrate the potential of the bECM hydrogel scaffolds to stimulate odontogenic differentiation of DPSCs.</p></div>", "links"=>[], "tags"=>["mineral deposition", "Von Kossa staining", "Von Kossa staining.ResultsWhen DPSCs", "GF", "surface marker expression", "TCPS", "mRNA expression levels", "DMP", "bECM hydrogel scaffolds", "culture conditions.SignificanceThese results", "Decellularized Bone Extracellular Matrix", "Hydrogel Scaffolds Derived", "tissue culture polystyrene", "MEPE mRNA expressions", "Human Dental Pulp Stem Cells", "bECM hydrogels", "matrix extracellular phosphoglycoprotein", "bone extracellular matrix", "odontogenic differentiation", "DSPP"], "article_id"=>2342266, "categories"=>["Biochemistry", "Space Science", "Medicine", "Microbiology", "Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Chemical Sciences not elsewhere classified", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Infectious Diseases"], "users"=>["Francesco Paduano", "Massimo Marrelli", "Lisa J. White", "Kevin M. Shakesheff", "Marco Tatullo"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0148225.s001", "https://dx.doi.org/10.1371/journal.pone.0148225.s002", "https://dx.doi.org/10.1371/journal.pone.0148225.s003"], "stats"=>{"downloads"=>3, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Odontogenic_Differentiation_of_Human_Dental_Pulp_Stem_Cells_on_Hydrogel_Scaffolds_Derived_from_Decellularized_Bone_Extracellular_Matrix_and_Collagen_Type_I/2342266", "title"=>"Odontogenic Differentiation of Human Dental Pulp Stem Cells on Hydrogel Scaffolds Derived from Decellularized Bone Extracellular Matrix and Collagen Type I", "pos_in_sequence"=>1, "defined_type"=>4, "published_date"=>"2016-02-16 07:45:02"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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