Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model
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{"title"=>"Fluorescence in vivo hybridization (FIVH) for detection of helicobacter pylori infection in a C57BL/6 mouse model", "type"=>"journal", "authors"=>[{"first_name"=>"Sílvia", "last_name"=>"Fontenete", "scopus_author_id"=>"55216563200"}, {"first_name"=>"Marina", "last_name"=>"Leite", "scopus_author_id"=>"56436933000"}, {"first_name"=>"Davie", "last_name"=>"Cappoen", "scopus_author_id"=>"54683685800"}, {"first_name"=>"Rita", "last_name"=>"Santos", "scopus_author_id"=>"56303632700"}, {"first_name"=>"Chris", "last_name"=>"Van Ginneken", "scopus_author_id"=>"26656407800"}, {"first_name"=>"Céu", "last_name"=>"Figueiredo", "scopus_author_id"=>"7006520927"}, {"first_name"=>"Jesper", "last_name"=>"Wengel", "scopus_author_id"=>"7103094424"}, {"first_name"=>"Paul", "last_name"=>"Cos", "scopus_author_id"=>"6602130431"}, {"first_name"=>"Nuno Filipe", "last_name"=>"Azevedo", "scopus_author_id"=>"8092231700"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"608547208", "sgr"=>"84959420492", "issn"=>"19326203", "pmid"=>"26848853", "scopus"=>"2-s2.0-84959420492", "doi"=>"10.1371/journal.pone.0148353", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"9e2bd86a-115e-35cd-9e15-705cf6657c78", "abstract"=>"INTRODUCTION: In this study, we applied fluorescence in vivo hybridization (FIVH) using locked nucleic acid (LNA) probes targeting the bacterial rRNA gene for in vivo detection of H. pylori infecting the C57BL/6 mouse model. A previously designed Cy3_HP_LNA/2OMe_PS probe, complementary to a sequence of the H. pylori 16S rRNA gene, was used. First, the potential cytotoxicity and genotoxicity of the probe was assessed by commercial assays. Further, the performance of the probe for detecting H. pylori at different pH conditions was tested in vitro, using fluorescence in situ hybridization (FISH). Finally, the efficiency of FIVH to detect H. pylori SS1 strain in C57BL/6 infected mice was evaluated ex vivo in mucus samples, in cryosections and paraffin-embedded sections by epifluorescence and confocal microscopy. RESULTS: H. pylori SS1 strain infecting C57BL/6 mice was successfully detected by the Cy3_HP_LNA/2OMe_PS probe in the mucus, attached to gastric epithelial cells and colonizing the gastric pits. The specificity of the probe for H. pylori was confirmed by microscopy. CONCLUSIONS: In the future this methodology can be used in combination with a confocal laser endomicroscope for in vivo diagnosis of H. pylori infection using fluorescent LNA probes, which would be helpful to obtain an immediate diagnosis. Our results proved for the first time that FIVH method is applicable inside the body of a higher-order animal.", "link"=>"http://www.mendeley.com/research/fluorescence-vivo-hybridization-fivh-detection-helicobacter-pylori-infection-c57bl6-mouse-model", "reader_count"=>19, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>3, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>3, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Mathematics"=>1, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>6, "Philosophy"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>1}, "Philosophy"=>{"Philosophy"=>1}}, "reader_count_by_country"=>{"Thailand"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4456843"], "description"=>"<p>Scatter plot of CFU for each animal, with bars representing the mean value.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762881, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Viable_bacterial_counts_CFU_colony_forming_units_from_stomachs_of_mice_infected_with_i_H_i_i_pylori_i_SS1_strain_for_2_weeks_/2762881", "title"=>"Viable bacterial counts (CFU, colony-forming units) from stomachs of mice infected with <i>H</i>. <i>pylori</i> SS1 strain for 2 weeks.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4240027", "https://ndownloader.figshare.com/files/4240030", "https://ndownloader.figshare.com/files/4240033", "https://ndownloader.figshare.com/files/4456783"], "description"=>"<div><p>Introduction</p><p>In this study, we applied fluorescence <i>in vivo</i> hybridization (FIVH) using locked nucleic acid (LNA) probes targeting the bacterial rRNA gene for in vivo detection of H. pylori infecting the C57BL/6 mouse model. A previously designed Cy3_HP_LNA/2OMe_PS probe, complementary to a sequence of the <i>H</i>. <i>pylori</i> 16S rRNA gene, was used. First, the potential cytotoxicity and genotoxicity of the probe was assessed by commercial assays. Further, the performance of the probe for detecting <i>H</i>. <i>pylori</i> at different pH conditions was tested <i>in vitro</i>, using fluorescence <i>in situ</i> hybridization (FISH). Finally, the efficiency of FIVH to detect <i>H</i>. <i>pylori</i> SS1 strain in C57BL/6 infected mice was evaluated <i>ex vivo</i> in mucus samples, in cryosections and paraffin-embedded sections by epifluorescence and confocal microscopy.</p><p>Results</p><p><i>H</i>. <i>pylori SS1</i> strain infecting C57BL/6 mice was successfully detected by the Cy3_HP_LNA/2OMe_PS probe in the mucus, attached to gastric epithelial cells and colonizing the gastric pits. The specificity of the probe for <i>H</i>. <i>pylori</i> was confirmed by microscopy.</p><p>Conclusions</p><p>In the future this methodology can be used in combination with a confocal laser endomicroscope for <i>in vivo</i> diagnosis of <i>H</i>. <i>pylori</i> infection using fluorescent LNA probes, which would be helpful to obtain an immediate diagnosis. Our results proved for the first time that FIVH method is applicable inside the body of a higher-order animal.</p></div>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2592706, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0148353.s001", "https://dx.doi.org/10.1371/journal.pone.0148353.s002", "https://dx.doi.org/10.1371/journal.pone.0148353.s003", "https://dx.doi.org/10.1371/journal.pone.0148353.s004"], "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Fluorescence_i_In_Vivo_i_Hybridization_FIVH_for_Detection_of_i_Helicobacter_pylori_i_Infection_in_a_C57BL_6_Mouse_Model/2592706", "title"=>"Fluorescence <i>In Vivo</i> Hybridization (FIVH) for Detection of <i>Helicobacter pylori</i> Infection in a C57BL/6 Mouse Model", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456846"], "description"=>"<p>Samples were collected and visualized directly using the epifluorescence microscope. Arrows indicate free-swimming <i>H</i>. <i>pylori</i> in the surface of mice gastric mucus. All images were taken at equal exposure times and are representative of the respective test group. Channels red, green and DAPI are overlapped. Scale bars: 10 μm.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762884, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Detection_of_i_H_i_i_pylori_SS1_i_in_the_surface_of_gastric_mucus_from_mice_subjected_to_FIVH_with_0_5_M_A_and_B_group_V_and_2_M_C_and_D_group_VI_of_the_Cy3_HP_LNA_2OMe_PS_probe_/2762884", "title"=>"Detection of <i>H</i>. <i>pylori SS1</i> in the surface of gastric mucus from mice subjected to FIVH with 0.5 μM (A and B; group V) and 2 μM (C and D; group VI) of the Cy3_HP_LNA/2OMe_PS probe.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456849"], "description"=>"<p>A and B: mice from group V (0.5 μM of the Cy3_HP_LNA/2OMe_PS probe), C and D: group VI (2 μM of the Cy3_HP_LNA/2OMe _PS probe). Arrows indicate the presence of <i>H</i>. <i>pylori</i> infecting the gastric mucosa. All images were taken at equal exposure times, with overlapping of the red, green and DAPI channels. All the images from each group are representative of <i>n</i> = 6 mice. A and C- scale bars: 50 μm. B and D- scale bars: 10 μm.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762887, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g007", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Detection_of_i_H_i_i_pylori_SS1_i_in_frozen_sections_of_gastric_mucosa_from_mice_subjected_to_FIVH_30_min_before_euthanasia_/2762887", "title"=>"Detection of <i>H</i>. <i>pylori SS1</i> in frozen sections of gastric mucosa from mice subjected to FIVH, 30 min before euthanasia.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456852"], "description"=>"<p>A and C. Fluorescence images of the detection in the surface of the gastric mucosa. B and D. Fluorescence images of the detection in the epithelium. A-B images obtained from mouse stomachs from group V. C-D images obtained from mouse stomachs from group VI. Red, green and DAPI channels are overlapped. All the images from each group are representative of <i>n</i> = 6 mice. A, B and C scale bars: 10 μm; D scale bars: 50 μm.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762890, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g008", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Detection_of_i_H_i_i_pylori_SS1_i_in_paraffin_sections_of_gastric_mucosa_from_mice_test_groups_subjected_to_FIVH_with_the_Cy3_HP_LNA_2OMe_PS_probe_30_min_before_being_sacrificed_/2762890", "title"=>"Detection of <i>H</i>. <i>pylori SS1</i> in paraffin sections of gastric mucosa from mice test groups subjected to FIVH with the Cy3_HP_LNA/2OMe_PS probe 30 min before being sacrificed.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456795"], "description"=>"<p>A. Four control groups with <i>n</i> = 3 (group I to IV) were used to evaluate the background of the vehicle (group I) and tissue (group II) and the specificity of the Cy3_HyP_LNA/2’OMe probe (groups III and IV). Two tests groups (<i>n</i> = 6, each group) were used with different probe concentrations, 0.5 μM (group V) and 2 μM (group VII). B. Animal study protocol depicting <i>H</i>. <i>pylori</i> inoculation, time of infection in C57BL/6 mice, and FIVH after 15 days post-infection.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762860, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/FIVH_scheme_used_for_detecting_i_H_i_i_pylori_i_in_C57BL_6_mice_/2762860", "title"=>"FIVH scheme used for detecting <i>H</i>. <i>pylori</i> in C57BL/6 mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456855"], "description"=>"<p>A. Sample of gastric mucus. B. Frozen section of mouse gastric mucosa. C. Paraffin section of mouse gastric mucosa. Detection of <i>H</i>. <i>pylori SS1</i> by the Cy3-labeled probe is depicted by white arrows. A and B: Red, green and DAPI channels were overlapped. C. Red and DAPI channels are overlapped. All the images from each group are representative of <i>n</i> = 3 mice. Scale bars: 10 μm.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762893, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g009", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Retention_of_the_Cy3_HP_LNA_2OMe_PS_probe_in_the_mouse_stomach_infected_with_i_H_i_i_pylori_i_SS1_for_5_days_and_subjected_to_a_FIVH_period_of_24_hrs_before_being_sacrificed_/2762893", "title"=>"Retention of the Cy3_HP_LNA/2OMe_PS probe in the mouse stomach infected with <i>H</i>. <i>pylori</i> SS1 for 5 days, and subjected to a FIVH period of 24 hrs before being sacrificed.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456807"], "description"=>"<p>AGS cells were treated with a range of concentrations of the Cy3_HP_LNA/2OMe_PS probe for 24 h (0.4 μM to 2 μM). Results are expressed as the mean ± SEM of three independent experiments, performed in triplicate; No statistical significant differences were found regarding probe-treated <i>vs</i> untreated control cells (<i>p>0</i>.<i>05</i> by ANOVA).</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762863, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Effect_of_the_Cy3_HP_LNA_2OMe_PS_probe_on_viability_of_AGS_gastric_epithelial_cells_using_the_MTS_assay_/2762863", "title"=>"Effect of the Cy3_HP_LNA/2OMe_PS probe on viability of AGS gastric epithelial cells using the MTS assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456858"], "description"=>"<p>Cy3_HP_LNA/2OMe_PS is a phosphorothioate oligomer (PS backbones), Cy3 (Cyanine).</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762896, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Designation_and_sequence_of_probe_containing_locked_nucleic_acid_LNA_with_L_superscript_and_2_O_methyl_RNA_2_OMe_in_boldface_nucleotide_monomers_/2762896", "title"=>"Designation and sequence of probe containing locked nucleic acid (LNA; with L superscript) and 2’-O-methyl-RNA (2’-OMe; in boldface) nucleotide monomers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456822"], "description"=>"<p>Results are expressed as the signal-to-noise (S/N) <i>ratio</i> between exposed and unexposed VITOTOX<sup>®</sup> test bacteria (Genox or Cytox strain) in the absence or presence of S9 mix. Bap:benzo[<i>α</i>]pyrene, the positive control, only turns genotoxic after S9 metabolisation.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762872, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/VITOTOX_sup_sup_assay_for_detection_of_signs_of_genotoxicity_caused_by_the_Cy3_HyP_LNA_2_OMe_probe_/2762872", "title"=>"VITOTOX<sup>®</sup> assay for detection of signs of genotoxicity caused by the Cy3_HyP_LNA/2’OMe probe.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/4456834"], "description"=>"<p>A to C—FISH protocol performed on smears of pure cultures of <i>H</i>. <i>pylori</i> SS1 incubated with 0.2 μM of probe. D to F—FISH protocol on smears of <i>H</i>. <i>pylori</i> SS1 cultures without probe used as negative control. A and D—Experiments performed at pH 2. B and E—Experiments performed at pH 4. C and F—Experiments performed at pH 7. All images were taken at equal exposure times. Scale bar: 10 μm.</p>", "links"=>[], "tags"=>["vivo hybridization", "pylori 16 S rRNA gene", "C 57BL Mouse Model IntroductionIn", "vivo diagnosis", "LNA probes", "confocal laser endomicroscope", "mucus samples", "rRNA gene", "confocal microscopy.ResultsH", "epithelial cells", "C 57BL", "FIVH method", "pylori SS 1 strain", "Vivo Hybridization", "Helicobacter pylori Infection", "vivo detection", "pylori infection", "C 57BL mice", "C 57BL mouse model", "pH conditions"], "article_id"=>2762878, "categories"=>["Biochemistry", "Space Science", "Microbiology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Virology"], "users"=>["Sílvia Fontenete", "Marina Leite", "Davie Cappoen", "Rita Santos", "Chris Van Ginneken", "Céu Figueiredo", "Jesper Wengel", "Paul Cos", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0148353.g004", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Detection_of_i_H_i_i_pylori_SS1_i_in_slides_by_fluorescence_i_in_situ_i_hybridization_FISH_using_the_Cy3_HP_LNA_2OMe_PS_probe_at_different_pH_values_analyzed_by_epifluorescence_microscopy_/2762878", "title"=>"Detection of <i>H</i>. <i>pylori SS1</i> in slides, by fluorescence <i>in situ</i> hybridization (FISH) using the Cy3_HP_LNA/2OMe_PS probe at different pH values, analyzed by epifluorescence microscopy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2016-02-05 21:23:34"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"27", "full-text"=>"31", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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