In-Situ Observation of Membrane Protein Folding during Cell-Free Expression
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{"title"=>"In-situ observation of membrane protein folding during cell-free expression", "type"=>"journal", "authors"=>[{"first_name"=>"Axel", "last_name"=>"Baumann", "scopus_author_id"=>"57188564469"}, {"first_name"=>"Silke", "last_name"=>"Kerruth", "scopus_author_id"=>"25629596400"}, {"first_name"=>"Jörg", "last_name"=>"Fitter", "scopus_author_id"=>"56135849800"}, {"first_name"=>"Georg", "last_name"=>"Büldt", "scopus_author_id"=>"7003894783"}, {"first_name"=>"Joachim", "last_name"=>"Heberle", "scopus_author_id"=>"7004170732"}, {"first_name"=>"Ramona", "last_name"=>"Schlesinger", "scopus_author_id"=>"57192176113"}, {"first_name"=>"Kenichi", "last_name"=>"Ataka", "scopus_author_id"=>"35583669200"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84961625936", "doi"=>"10.1371/journal.pone.0151051", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"26978519", "issn"=>"19326203", "scopus"=>"2-s2.0-84961625936", "pui"=>"609076924"}, "id"=>"4e0d963c-4254-34e8-a8a9-34d37576108f", "abstract"=>"Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity of a living cell. Here, we present a novel approach to trace folding and insertion of a nascent membrane protein leaving the ribosome and penetrating the bilayer. Surface Enhanced IR Absorption Spectroscopy selectively monitored insertion and folding of membrane proteins during cell-free expression in a label-free and non-invasive manner. Protein synthesis was performed in an optical cell containing a prism covered with a thin gold film with nanodiscs on top, providing an artificial lipid bilayer for folding. In a pilot experiment, the folding pathway of bacteriorhodopsin via various secondary and tertiary structures was visualized. Thus, a methodology is established with which the folding reaction of other more complex membrane proteins can be observed during protein biosynthesis (in situ and in operando) at molecular resolution.", "link"=>"http://www.mendeley.com/research/insitu-observation-membrane-protein-folding-during-cellfree-expression", "reader_count"=>31, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>4, "Other"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>4, "Other"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>12, "Materials Science"=>1, "Agricultural and Biological Sciences"=>7, "Physics and Astronomy"=>5, "Chemical Engineering"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Materials Science"=>{"Materials Science"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>2}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Germany"=>3}, "group_count"=>2}

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{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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