Lamellipodin-Deficient Mice: A Model of Rectal Carcinoma
Publication Date
April 05, 2016
Journal
PLOS ONE
Authors
Cassandra L. Miller, Sureshkumar Muthupalani, Zeli Shen, Frauke Drees, et al
Volume
11
Issue
4
Pages
e0152940
DOI
https://dx.plos.org/10.1371/journal.pone.0152940
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0152940
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/27045955
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4821566
Europe PMC
http://europepmc.org/abstract/MED/27045955
Web of Science
000373599600050
Scopus
84962921230
Mendeley
http://www.mendeley.com/research/lamellipodindeficient-mice-model-rectal-carcinoma
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Lamellipodin-deficient mice: A model of rectal carcinoma", "type"=>"journal", "authors"=>[{"first_name"=>"Cassandra L.", "last_name"=>"Miller", "scopus_author_id"=>"56237213900"}, {"first_name"=>"Sureshkumar", "last_name"=>"Muthupalani", "scopus_author_id"=>"26534624500"}, {"first_name"=>"Zeli", "last_name"=>"Shen", "scopus_author_id"=>"7403320091"}, {"first_name"=>"Frauke", "last_name"=>"Drees", "scopus_author_id"=>"9639946300"}, {"first_name"=>"Zhongming", "last_name"=>"Ge", "scopus_author_id"=>"7202139983"}, {"first_name"=>"Yan", "last_name"=>"Feng", "scopus_author_id"=>"56402178600"}, {"first_name"=>"Xiaowei", "last_name"=>"Chen", "scopus_author_id"=>"57102238900"}, {"first_name"=>"Guanyu", "last_name"=>"Gong", "scopus_author_id"=>"56542599500"}, {"first_name"=>"Karan K.", "last_name"=>"Nagar", "scopus_author_id"=>"56481490100"}, {"first_name"=>"Timothy C.", "last_name"=>"Wang", "scopus_author_id"=>"35199244700"}, {"first_name"=>"Frank B.", "last_name"=>"Gertler", "scopus_author_id"=>"7003755623"}, {"first_name"=>"James G.", "last_name"=>"Fox", "scopus_author_id"=>"56691286100"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84962921230", "sgr"=>"84962921230", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0152940", "pmid"=>"27045955", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pui"=>"609632558"}, "id"=>"df29fcb5-d97a-35dd-8902-581201dea120", "abstract"=>"During a survey of clinical rectal prolapse (RP) cases in the mouse population at MIT animal research facilities, a high incidence of RP in the lamellipodin knock-out strain, C57BL/6-Raph1tm1Fbg (Lpd-/-) was documented. Upon further investigation, the Lpd-/- colony was found to be infected with multiple endemic enterohepatic Helicobacter species (EHS). Lpd-/- mice, a transgenic mouse strain produced at MIT, have not previously shown a distinct immune phenotype and are not highly susceptible to other opportunistic infections. Predominantly male Lpd-/- mice with RP exhibited lesions consistent with invasive rectal carcinoma concomitant to clinically evident RP. Multiple inflammatory cytokines, CD11b+Gr1+ myeloid-derived suppressor cell (MDSC) populations, and epithelial cells positive for a DNA damage biomarker, H2AX, were elevated in affected tissue, supporting their role in the neoplastic process. An evaluation of Lpd-/- mice with RP compared to EHS-infected, but clinically normal (CN) Lpd-/- animals indicated that all of these mice exhibit some degree of lower bowel inflammation; however, mice with prolapses had significantly higher degree of focal lesions at the colo-rectal junction. When Helicobacter spp. infections were eliminated in Lpd-/- mice by embryo transfer rederivation, the disease phenotype was abrogated, implicating EHS as a contributing factor in the development of rectal carcinoma. Here we describe lesions in Lpd-/- male mice consistent with a focal inflammation-induced neoplastic transformation and propose this strain as a mouse model of rectal carcinoma.", "link"=>"http://www.mendeley.com/research/lamellipodindeficient-mice-model-rectal-carcinoma", "reader_count"=>10, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>3, "Materials Science"=>1, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>2, "Veterinary Science and Veterinary Medicine"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84962921230"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84962921230?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962921230&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84962921230&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84962921230", "dc:identifier"=>"SCOPUS_ID:84962921230", "eid"=>"2-s2.0-84962921230", "dc:title"=>"Lamellipodin-deficient mice: A model of rectal carcinoma", "dc:creator"=>"Miller C.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"11", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2016-04-01", "prism:coverDisplayDate"=>"April 2016", "prism:doi"=>"10.1371/journal.pone.0152940", "citedby-count"=>"3", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Massachusetts Institute of Technology", "affiliation-city"=>"Cambridge", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Ltd.", "affiliation-city"=>"Boulder", "affiliation-country"=>"United States"}], "pubmed-id"=>"27045955", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0152940", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0152940", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"35", "xml_views"=>"2", "html_views"=>"162"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"163"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"144"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"150"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"308"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"4", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4917985"], "description"=>"<p>Proportions of myeloid cells increased significantly in LPD<sup>-/-</sup> RP mice blood, spleen, and rectal prolapse tissue. Left panel, representative flow plots of CD11b<sup>+</sup>Gr1<sup>+</sup> myeloid cells from various tissue samples compared RP and control mice, gating on CD45<sup>+</sup>. Right panel, quantification of CD11b<sup>+</sup>Gr1<sup>+</sup> myeloid cells in CD45<sup>+</sup> population (n = 3). Error bars, mean ± s.e.m. P values were derived from an unpaired, two-tailed Student’s t-test (* P<0.05; ** P<0.01). CN = Clinically Normal; RP = Rectal Prolapse.</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3157057, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Flow_analysis_of_CD45_sup_sup_CD11b_sup_sup_Gr1_sup_sup_myeloid_cells_/3157057", "title"=>"Flow analysis of CD45<sup>+</sup>CD11b<sup>+</sup>Gr1<sup>+</sup> myeloid cells.", "pos_in_sequence"=>7, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917871"], "description"=>"<p>(A) Comparison of Rectal/Colo-rectal Junction Histologic Scores. (B) Comparison of Distal Colon Histologic Scores. (C-F) Comparison of Histology Observed in Clinically Normal Lpd Mice (C&D) vs RP Lpd Mice (E&F). In the low and high magnification images of a representative clinically normal animal, the non- prolapsed rectum and colon are normal without any significant inflammation or other changes like hyperplasia or dysplasia. The low magnification image (E) of the clinically affected animal shows marked expansion of the prolapsed rectum and adjacent distal colon by inflammation, edema, glandular hyperplasia, and high grade dysplasia/neoplasia. High magnification of the prolapsed rectum (F) showing carcinomatous glands that have invaded deep into the underlying musculature. (Bar lengths = C: 400μm; D: 150μm; E: 400μm; F: 75μm).</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3156946, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Lamellipodin_Deficient_Mice_A_Model_of_Rectal_Carcinoma_Fig_3/3156946", "title"=>"Lamellipodin-Deficient Mice: A Model of Rectal Carcinoma - Fig 3", "pos_in_sequence"=>3, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917958"], "description"=>"<p>Total RNA prepared from rectal tissues of mice were evaluated by qPCR for expression levels of mRNA for select cytokines, which then were normalized to the expression of the house-keeping gene Gapdh. The Y axis represents the mean fold change (± standard deviation) of the mRNA levels in reference healthy controls. P values: *** <0.0001, * <0.026.</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3157033, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Lpd_sup_sup_mice_with_RP_produced_significantly_higher_mRNA_levels_of_rectal_inflammatory_mediators_compared_healthy_controls_/3157033", "title"=>"Lpd<sup>-/-</sup> mice with RP produced significantly higher mRNA levels of rectal inflammatory mediators compared healthy controls.", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917790"], "description"=>"<p>Results from 15 animals are shown, with AluI digest on the top, and the corresponding HhaI digest shown on the bottom for each sample. While HhaI digests all exhibit the typical banding pattern for <i>H</i>. <i>typhlonius</i>, there is variability in the AluI patterns. Rows 1, 2, 3, 8, 9, 10, and 14 are all characteristic of <i>H</i>. <i>typhlonius</i>, whereas the remaining samples showed patterns that were less readily identifiable, suggesting coinfections. These animals were later determined to be co-infected with <i>H</i>. <i>hepaticus</i> by species-specific PCR.</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3156874, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/RFLP_Patterns_for_EHS_Lpd_sup_sup_Mice_/3156874", "title"=>"RFLP Patterns for EHS+ Lpd<sup>-/-</sup> Mice.", "pos_in_sequence"=>1, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917928"], "description"=>"<p>(A) immunostaining of DNA damage marker H2AX in LPD -/- mice without EHS infection, LPD -/- mice with EHS infection and with rectal inflammation and prolapse, LPD -/- mice with EHS infection and without rectal inflammation and prolapse; (B) morphometric quantification of average frequency of H2AX positive cells per thousand enterocytes. For each animal, total 8–10 images were obtained and analyzed. Monoclonal rabbit anti-H2AX antibody was used to label cells bearing DNA damage. The secondary antibody was goat anti-Rabbit Ig(H+L), alexa fluor 568 conjugated. Animal number, group LPD -/- control uninfected mice = 6, group LPD -/- EHS-infected with RP = 17, group LPD -/- EHS-infected without RP = 14, group LPD +/- EHS-infected = 10. *** significant at < 0.001. Magnification = 200x; bar = 20μm.</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3157003, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Immunostaining_and_morphologic_analysis_of_DNA_damage_using_H2AX_/3157003", "title"=>"Immunostaining and morphologic analysis of DNA damage using H2AX.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917898"], "description"=>"<p>(A) The colo-rectal junction region of a clinically normal, EHS-positive mouse showing organisms adjacent to host cells in the distal GI tract; (B) Typical positive staining showing heavy colonization within the crypts of the colon; (C) Rectum of the same animal in B with organisms detected along the surface of abnormal prolapsed tissue; (D) Another prolapsed rectum with EHS dispersed within the abnormal edematous tissue. A combination of two genus-specific probes, HEL274 and HEL717, labeled with Cy3 were used. Magnification = 630x; bar = 20μm.</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3156973, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Fluorescent_in_situ_Hybridization_on_lower_bowel_of_Lpd_sup_sup_mice_/3156973", "title"=>"Fluorescent in situ Hybridization on lower bowel of Lpd<sup>-/-</sup> mice.", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/4917832"], "description"=>"<p>Levels of EHS colonization were determined by qPCR in the cecum and colon of both RP and CN mice. Organisms were quantified in the rectum only for RP mice, as the normal rectal tissue was less abundant and thus spared for histologic analysis. As expected, EHS were most abundant in the ceca for both groups of mice. Colonization was more variable and tended to decrease distally, yet EHS were still detected in all locations. (RP: Rectal prolapse; CN: Clinically normal).</p>", "links"=>[], "tags"=>["EHS", "enterohepatic Helicobacter species", "embryo transfer rederivation", "MDSC", "rectal carcinoma", "2AX", "CN", "MIT animal research facilities", "DNA damage biomarker", "RP", "strain", "lesion", "Lpd", "C 57BL Raph 1 tm 1Fbg"], "article_id"=>3156910, "categories"=>["Medicine", "Microbiology", "Cell Biology", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases"], "users"=>["Cassandra L. Miller", "Sureshkumar Muthupalani", "Zeli Shen", "Frauke Drees", "Zhongming Ge", "Yan Feng", "Xiaowei Chen", "Guanyu Gong", "Karan K. Nagar", "Timothy C. Wang", "Frank B. Gertler", "James G. Fox"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0152940.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Quantitation_of_EHS_in_the_Lower_Bowel_of_LPD_mice_/3156910", "title"=>"Quantitation of EHS in the Lower Bowel of LPD -/- mice.", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-04-05 08:26:29"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"31", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"31", "full-text"=>"46", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts