MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
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{"title"=>"MicroRNA-3646 contributes to docetaxel resistance in human breast cancer cells by GSK-3β/β-catenin signaling pathway", "type"=>"journal", "authors"=>[{"first_name"=>"Xiaohui", "last_name"=>"Zhang", "scopus_author_id"=>"37038959800"}, {"first_name"=>"Shanliang", "last_name"=>"Zhong", "scopus_author_id"=>"37000215000"}, {"first_name"=>"Yong", "last_name"=>"Xu", "scopus_author_id"=>"57188767389"}, {"first_name"=>"Dandan", "last_name"=>"Yu", "scopus_author_id"=>"56539103900"}, {"first_name"=>"Tengfei", "last_name"=>"Ma", "scopus_author_id"=>"56408908900"}, {"first_name"=>"Lin", "last_name"=>"Chen", "scopus_author_id"=>"57032046300"}, {"first_name"=>"Yang", "last_name"=>"Zhao", "scopus_author_id"=>"56150386900"}, {"first_name"=>"Xiu", "last_name"=>"Chen", "scopus_author_id"=>"56844664300"}, {"first_name"=>"Sujin", "last_name"=>"Yang", "scopus_author_id"=>"56843828900"}, {"first_name"=>"Yueqin", "last_name"=>"Wu", "scopus_author_id"=>"57188758715"}, {"first_name"=>"Jinhai", "last_name"=>"Tang", "scopus_author_id"=>"7404638892"}, {"first_name"=>"Jianhua", "last_name"=>"Zhao", "scopus_author_id"=>"55729942900"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27045586", "scopus"=>"2-s2.0-84962882030", "sgr"=>"84962882030", "doi"=>"10.1371/journal.pone.0153194", "pui"=>"609632757", "issn"=>"19326203"}, "id"=>"1f13ff42-2587-31c9-9c12-820240efa1b7", "abstract"=>"Acquisition of resistance to docetaxel (Doc) is one of the most important problems in treatment of breast cancer patients, but the underlying mechanisms are still not fully understood. In present study, Doc-resistant MDA-MB-231 and MCF-7 breast cancer cell lines (MDA-MB-231/Doc and MCF-7/Doc) were successfully established in vitro by gradually increasing Doc concentration on the basis of parental MDA-MB-231 and MCF-7 cell lines (MDA-MB-231/S and MCF-7/S). The potential miRNAs relevant to the Doc resistance were screened by miRNA microarray. We selected 5 upregulated miRNAs (has-miR-3646, has-miR-3658, has-miR-4438, has-miR-1246, and has-miR-574-3p) from the results of microarray for RT-qPCR validation. The results showed that expression level of miR-3646 in MDA-MB-231/Doc cells was significantly higher than in MDA-MB-231/S cells. Compared to MCF-7/S cells, miR-3646 expression was up-regulated in MCF-7/Doc cells. Further studies revealed that transfection of miR-3646 mimics into MDA-MB-231/S or MCF-7/S cells remarkably increased their drug resistance, in contrast, transfection of miR-3646 inhibitors into MDA-MB-231/Doc or MCF-7/Doc cells resulted in significant reduction of the drug resistance. By the pathway enrichment analyses for miR-3646, we found that GSK-3β/β-catenin signaling pathway was a significant pathway, in which GSK-3β was an essential member. RT-qPCR and Western blot results demonstrated that miR-3646 could regulate GSK-3β mRNA and protein expressions. Furthermore, a marked increase of both nuclear and cytoplasmic β-catenin expressions (with phosphorylated-β-catenin decrease) was observed in MDA-MB-231/Doc cells compared with MDA-MB-231/S cells, and their expression were positively related to miR-3646 and negatively correlated with GSK-3β. Taken together, our results suggest that miR-3646-mediated Doc resistance of breast cancer cells maybe, at least in part, through suppressing expression of GSK-3β and resultantly activating GSK-3β/β-catenin signaling pathway.", "link"=>"http://www.mendeley.com/research/microrna3646-contributes-docetaxel-resistance-human-breast-cancer-cells-gsk3%CE%B2%CE%B2catenin-signaling-path", "reader_count"=>10, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>3, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4918882"], "description"=>"<p><b>a,c</b> The IC50 value of Doc was determined after MDA-MB-231/S or MCF-7/S cells were transfected with miR-3646 mimics, NC or blank control for 48h using MTT assay (*<i>P</i><0.05). <b>b,d</b> After MDA-MB-231/Doc or MCF-7/S cells were transfected with miR-3646 inhibitors, NC or blank control for 48h, theIC50 value of Doc was determined by MTT assay (*<i>P</i><0.05).</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157639, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Effect_of_miR_3646_mimics_and_miR_3646_inhibitors_on_the_sensitivity_of_breast_cancer_cell_lines_to_Doc_/3157639", "title"=>"Effect of miR-3646 mimics and miR-3646 inhibitors on the sensitivity of breast cancer cell lines to Doc.", "pos_in_sequence"=>3, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4918744", "https://ndownloader.figshare.com/files/4918759"], "description"=>"<div><p>Acquisition of resistance to docetaxel (Doc) is one of the most important problems in treatment of breast cancer patients, but the underlying mechanisms are still not fully understood. In present study, Doc-resistant MDA-MB-231 and MCF-7 breast cancer cell lines (MDA-MB-231/Doc and MCF-7/Doc) were successfully established <i>in vitro</i> by gradually increasing Doc concentration on the basis of parental MDA-MB-231 and MCF-7 cell lines (MDA-MB-231/S and MCF-7/S). The potential miRNAs relevant to the Doc resistance were screened by miRNA microarray. We selected 5 upregulated miRNAs (has-miR-3646, has-miR-3658, has-miR-4438, has-miR-1246, and has-miR-574-3p) from the results of microarray for RT-qPCR validation. The results showed that expression level of miR-3646 in MDA-MB-231/Doc cells was significantly higher than in MDA-MB-231/S cells. Compared to MCF-7/S cells, miR-3646 expression was up-regulated in MCF-7/Doc cells. Further studies revealed that transfection of miR-3646 mimics into MDA-MB-231/S or MCF-7/S cells remarkably increased their drug resistance, in contrast, transfection of miR-3646 inhibitors into MDA-MB-231/Doc or MCF-7/Doc cells resulted in significant reduction of the drug resistance. By the pathway enrichment analyses for miR-3646, we found that GSK-3β/β-catenin signaling pathway was a significant pathway, in which GSK-3β was an essential member. RT-qPCR and Western blot results demonstrated that miR-3646 could regulate GSK-3β mRNA and protein expressions. Furthermore, a marked increase of both nuclear and cytoplasmic β-catenin expressions (with phosphorylated-β-catenin decrease) was observed in MDA-MB-231/Doc cells compared with MDA-MB-231/S cells, and their expression were positively related to miR-3646 and negatively correlated with GSK-3β. Taken together, our results suggest that miR-3646-mediated Doc resistance of breast cancer cells maybe, at least in part, through suppressing expression of GSK-3β and resultantly activating GSK-3β/β-catenin signaling pathway.</p></div>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157549, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0153194.s001", "https://dx.doi.org/10.1371/journal.pone.0153194.s002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/MicroRNA_3646_Contributes_to_Docetaxel_Resistance_in_Human_Breast_Cancer_Cells_by_GSK_3_Catenin_Signaling_Pathway/3157549", "title"=>"MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway", "pos_in_sequence"=>1, "defined_type"=>4, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4918921"], "description"=>"<p><b>a</b> The apoptotic rate and representative FACS figures of MDA-MB-231/S cells transfected with miR-3646 mimics were significantly decreased compared with NC and blank control (*<i>P</i><0.05). <b>b</b> The apoptotic rate and representative FACS figures of MDA-MB-231/Doc cells transfected with miR-3646 inhibitors were significantly augmented compared with NC and blank control (*<i>P</i><0.05).</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157681, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Flow_cytometric_analysis_of_apoptotic_MDA_MB_231_S_and_MDA_MB_231_Doc_cells_induced_by_Doc_/3157681", "title"=>"Flow cytometric analysis of apoptotic MDA-MB-231/S and MDA-MB-231/Doc cells induced by Doc.", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4919059"], "description"=>"<p><b>a,c</b> MDA-MB-231 cells displayed both nuclear and cytoplasmic β-catenin expression. The expression of β-catenin in MDA-MB-231/Doc cells was highly regulated as compared to the expression levels in MDA-MB-231/S cells. <b>b</b> The sensitive cells displayed increased nuclear and cytoplasmic β-catenin expression after transfection with miR-3646 mimics. <b>d</b> The resistant cells showed decreased nuclear and cytoplasmic β-catenin expression after transfection with miR-3646 inhibitors. Blue = nuclear staining; Red = β-catenin; Pink = merge.</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157780, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_catenin_immunofluorescence_in_breast_cancer_cells_/3157780", "title"=>"β-catenin immunofluorescence in breast cancer cells.", "pos_in_sequence"=>7, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4918999"], "description"=>"<p><b>a</b> Four complementary sites of miR-3646 in GSK-3β 3’UTR were predicted by bioinformatics analysis. <b>b</b> Relative mRNA and protein expression of GSK-3β in MDA-MB-231/S and MDA-MB-231/Doc cells. <b>c</b> Relative mRNA and protein expression of GSK-3β in MDA-MB-231/S cells transfected with miR-3646 mimics, NC and blank control. <b>d</b> Relative mRNA and protein expression of GSK-3β in MDA-MB-231/Doc cells transfected with miR-3646 inhibitors, NC and blank control.</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157750, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/GSK_3_was_a_candidate_target_of_miRNA_3646_/3157750", "title"=>"GSK-3β was a candidate target of miRNA-3646.", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4919101"], "description"=>"<p><b>a</b> Protein expression of β-catenin and P-β-catenin in MDA-MB-231/S and MDA-MB-231/Doc cells. <b>b</b> Protein expression of β-catenin and P-β-catenin in MDA-MB-231/S cells transfected with miR-3646 mimics, NC and blank control. <b>c</b> Protein expression of β-catenin and P-β-catenin protein in MDA-MB-231/Doc cells transfected with miR-3646 inhibitors, NC and blank control. <b>d</b> Relative mRNA expression of β-catenin in MDA-MB-231/S and MDA-MB-231/Doc cells (*<i>P</i><0.05). <b>e</b> Relative mRNA expression of β-catenin in MDA-MB-231/S cells transfected with miR-3646 mimics, NC and blank control (*<i>P</i><0.05). <b>f</b> Relative mRNA expression of β-catenin in MDA-MB-231/Doc cells transfected with miR-3646 inhibitors, NC and blank control (*<i>P</i><0.05).</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157816, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/RT_qPCR_and_Western_blot_analysis_of_catenin_and_P_catenin_expression_in_MDA_MB_231_S_and_MDA_MB_231_Doc_cells_/3157816", "title"=>"RT-qPCR and Western blot analysis of β-catenin and P-β-catenin expression in MDA-MB-231/S and MDA-MB-231/Doc cells.", "pos_in_sequence"=>8, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4918828"], "description"=>"<p><b>a</b> Relative expression of 5 miRNAs in MDA-MB-231/S and MDA-MB-231/Doc cells. <b>b,e</b> Relative expression of miR-3646 in MDA-MB-231/S or MCF-7/S cells transfected with miRNA-3646 mimics and negative control of mimics (NC) (*<i>P</i><0.05). <b>c,f</b> Relative expression of miR-3646 in MDA-MB-231/Doc or MCF-7/Doc cells transfected with miR-3646 inhibitors and negative control of inhibitors (NC) (*<i>P</i><0.05). <b>d</b> Relative expression miR-3646 of in MCF-7/S and MCF-7/Doc cells. <b>g</b> The transfection efficiency of electroporation was determined by green fluorescence.</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157594, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Relative_expression_level_of_miRNAs_in_the_breast_cancer_cells_/3157594", "title"=>"Relative expression level of miRNAs in the breast cancer cells.", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/4918966"], "description"=>"<p><b>a</b> The apoptotic rate and representative FACS figures in MCF-7/S cells transfected with miR-3646 mimics, NC and blank control (*<i>P</i><0.05). <b>b</b> The apoptotic rate and representative FACS figures in MCF-7/Doc cells transfected with miR-3646 inhibitors, NC and blank control (*<i>P</i><0.05).</p>", "links"=>[], "tags"=>["GSK", "Human Breast Cancer Cells", "MCF", "5 upregulated miRNAs", "breast cancer cells", "drug resistance", "breast cancer patients", "Doc", "Western blot results", "pathway enrichment analyses", "expression"], "article_id"=>3157723, "categories"=>["Biophysics", "Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Immunology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology", "Plant Biology"], "users"=>["Xiaohui Zhang", "Shanliang Zhong", "Yong Xu", "Dandan Yu", "Tengfei Ma", "Lin Chen", "Yang Zhao", "Xiu Chen", "Sujin Yang", "Yueqin Wu", "Jinhai Tang", "Jianhua Zhao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153194.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Flow_cytometry_assessment_of_apoptotic_MCF_7_cells_induced_by_Doc_was_determined_using_APC_Annexin_V_/3157723", "title"=>"Flow cytometry assessment of apoptotic MCF-7 cells induced by Doc was determined using APC Annexin V.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-04-05 08:31:19"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"33", "full-text"=>"35", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
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  • {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
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  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
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  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
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  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"1", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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