Transgenic Sugarcane with a cry1Ac Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer
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{"title"=>"Transgenic sugarcane with a cry1Ac gene exhibited better phenotypic traits and enhanced resistance against sugarcane borer", "type"=>"journal", "authors"=>[{"first_name"=>"Shiwu", "last_name"=>"Gao", "scopus_author_id"=>"36988919500"}, {"first_name"=>"Yingying", "last_name"=>"Yang", "scopus_author_id"=>"57188555518"}, {"first_name"=>"Chunfeng", "last_name"=>"Wang", "scopus_author_id"=>"57158583200"}, {"first_name"=>"Jinlong", "last_name"=>"Guo", "scopus_author_id"=>"55745785100"}, {"first_name"=>"Dinggang", "last_name"=>"Zhou", "scopus_author_id"=>"56168393300"}, {"first_name"=>"Qibin", "last_name"=>"Wu", "scopus_author_id"=>"55510888700"}, {"first_name"=>"Yachun", "last_name"=>"Su", "scopus_author_id"=>"55511172700"}, {"first_name"=>"Liping", "last_name"=>"Xu", "scopus_author_id"=>"35212648500"}, {"first_name"=>"Youxiong", "last_name"=>"Que", "scopus_author_id"=>"25028730100"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84964579478", "issn"=>"19326203", "pui"=>"609983098", "sgr"=>"84964579478", "doi"=>"10.1371/journal.pone.0153929"}, "id"=>"9b4c9966-f11e-3921-8d1b-dbbf6c317033", "abstract"=>"We developed sugarcane plants with improved resistance to the sugarcane borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring the cry1Ac gene and the selectable marker gene, bar, was constructed. This construct was introduced into the sugar-cane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Basta. Plantlets were then screened by PCR based on the presence of cry1Ac and 14 cry1Ac positive plantlets were identified. Real-time quantitative PCR (RT-qPCR) revealed that the copy number of cry1Ac gene in the transgenic lines varied from 1 to 148. ELISA analysis showed that Cry1Ac protein levels in 7 transgenic lines ranged from 0.85 μg/FWg to 70.92 μg/FWg in leaves and 0.04 μg/FWg to 7.22 μg/FWg in stems, and negatively correlated to the rate of insect damage that ranged from 36.67% to 13.33%, respectively. Agronomic traits of six transgenic sugarcane lines with medium copy numbers were similar to the non-transgenic parental line. However, phe-notype was poor in lines with high or low copy numbers. Compared to the non-transgenic control plants, all transgenic lines with medium copy numbers had relatively equal or lower sucrose yield and significantly improved sugarcane borer resistance, which lowered sus-ceptibility to damage by insects. This suggests that the transgenic sugarcane lines harbor-ing medium copy numbers of the cry1Ac gene may have significantly higher resistance to sugarcane borer but the sugarcane yield in these lines is similar to the non-transgenic con-trol thus making them superior to the control lines.", "link"=>"http://www.mendeley.com/research/transgenic-sugarcane-cry1ac-gene-exhibited-better-phenotypic-traits-enhanced-resistance-against-suga", "reader_count"=>25, "reader_count_by_academic_status"=>{"Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>16, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Brazil"=>1}, "group_count"=>3}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/4971556"], "description"=>"<p>Damage comparison between transgenic and non-transgenic sugarcane stalks.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185506, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Damage_comparison_between_transgenic_and_non_transgenic_sugarcane_stalks_/3185506", "title"=>"Damage comparison between transgenic and non-transgenic sugarcane stalks.", "pos_in_sequence"=>8, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971364", "https://ndownloader.figshare.com/files/4971370", "https://ndownloader.figshare.com/files/4971379", "https://ndownloader.figshare.com/files/4971388"], "description"=>"<div><p>We developed sugarcane plants with improved resistance to the sugarcane borer, <i>Diatraea saccharalis</i> (F). An expression vector pGcry1Ac0229, harboring the <i>cry1Ac</i> gene and the selectable marker gene, <i>bar</i>, was constructed. This construct was introduced into the sugarcane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Basta. Plantlets were then screened by PCR based on the presence of <i>cry1Ac</i> and 14 <i>cry1Ac</i> positive plantlets were identified. Real-time quantitative PCR (RT-qPCR) revealed that the copy number of <i>cry1Ac</i> gene in the transgenic lines varied from 1 to 148. ELISA analysis showed that Cry1Ac protein levels in 7 transgenic lines ranged from 0.85 μg/FWg to 70.92 μg/FWg in leaves and 0.04 μg/FWg to 7.22 μg/FWg in stems, and negatively correlated to the rate of insect damage that ranged from 36.67% to 13.33%, respectively. Agronomic traits of six transgenic sugarcane lines with medium copy numbers were similar to the non-transgenic parental line. However, phenotype was poor in lines with high or low copy numbers. Compared to the non-transgenic control plants, all transgenic lines with medium copy numbers had relatively equal or lower sucrose yield and significantly improved sugarcane borer resistance, which lowered susceptibility to damage by insects. This suggests that the transgenic sugarcane lines harboring medium copy numbers of the <i>cry1Ac</i> gene may have significantly higher resistance to sugarcane borer but the sugarcane yield in these lines is similar to the non-transgenic control thus making them superior to the control lines.</p></div>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185380, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0153929.s001", "https://dx.doi.org/10.1371/journal.pone.0153929.s002", "https://dx.doi.org/10.1371/journal.pone.0153929.s003", "https://dx.doi.org/10.1371/journal.pone.0153929.s004"], "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Transgenic_Sugarcane_with_a_i_cry1Ac_i_Gene_Exhibited_Better_Phenotypic_Traits_and_Enhanced_Resistance_against_Sugarcane_Borer/3185380", "title"=>"Transgenic Sugarcane with a <i>cry1Ac</i> Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer", "pos_in_sequence"=>1, "defined_type"=>4, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971511"], "description"=>"<p><b>A</b>: Symptoms of non-transgenic sugarcane plantlets challenged with sugarcane borers; <b>B</b>: Symptoms of transgenic sugarcane plantlets challenged with sugarcane borers; <b>C</b>: Sugarcane borer feeding with non-transgenic sugarcane plantlets; <b>D</b>: Sugarcane borer feeding with transgenic sugarcane plantlets.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185479, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Bioassay_in_the_seedling_stage_/3185479", "title"=>"Bioassay in the seedling stage.", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971565"], "description"=>"<p>Copy number estimation in different transgenic sugarcane lines with a <i>cry1Ac</i> gene.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185512, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Copy_number_estimation_in_different_transgenic_sugarcane_lines_with_a_i_cry1Ac_i_gene_/3185512", "title"=>"Copy number estimation in different transgenic sugarcane lines with a <i>cry1Ac</i> gene.", "pos_in_sequence"=>9, "defined_type"=>3, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971445"], "description"=>"<p>The standard curve of Cry1Ac protein constructed by ELISA.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185449, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_standard_curve_of_Cry1Ac_protein_constructed_by_ELISA_/3185449", "title"=>"The standard curve of Cry1Ac protein constructed by ELISA.", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971403"], "description"=>"<p>Partially regenerated plantlets with PPT resistance in differentiation culture.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185410, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Partially_regenerated_plantlets_with_PPT_resistance_in_differentiation_culture_/3185410", "title"=>"Partially regenerated plantlets with PPT resistance in differentiation culture.", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}
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  • {"files"=>["https://ndownloader.figshare.com/files/4971481"], "description"=>"<p>Cry1Ac protein expression in the leaves and stems of 14 different transgenic sugarcane lines detected by ELISA.</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185467, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Cry1Ac_protein_expression_in_the_leaves_and_stems_of_14_different_transgenic_sugarcane_lines_detected_by_ELISA_/3185467", "title"=>"Cry1Ac protein expression in the leaves and stems of 14 different transgenic sugarcane lines detected by ELISA.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/4971535"], "description"=>"<p><b>A</b>: Leaf symptoms; <b>B</b>: Stem symptoms (aspect); <b>C</b>: Stem symptoms (inside stalk).</p>", "links"=>[], "tags"=>["Cry 1Ac protein levels", "14 cry 1Ac", "medium copy numbers", "PCR", "cry 1Ac Gene", "Better Phenotypic Traits", "sugarcane borer resistance", "cry 1Ac gene", "selectable marker gene", "148. ELISA analysis", "sugarcane cultivar FN 15", "PPT", "sugarcane borer", "expression vector pGcry 1Ac", "sugarcane lines"], "article_id"=>3185500, "categories"=>["Cell Biology", "Genetics", "Molecular Biology", "Biotechnology", "Environmental Sciences not elsewhere classified", "Ecology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Plant Biology"], "users"=>["Shiwu Gao", "Yingying Yang", "Chunfeng Wang", "Jinlong Guo", "Dinggang Zhou", "Qibin Wu", "Yachun Su", "Liping Xu", "Youxiong Que"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0153929.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Typical_symptoms_caused_by_natural_stem_borer_infestation_in_the_field_/3185500", "title"=>"Typical symptoms caused by natural stem borer infestation in the field.", "pos_in_sequence"=>7, "defined_type"=>1, "published_date"=>"2016-04-19 05:23:37"}

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Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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