Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells
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{"title"=>"Loss of Pcgf5 affects global H2A monoubiquitination but not the function of hematopoietic stem and progenitor cells", "type"=>"journal", "authors"=>[{"first_name"=>"Sha", "last_name"=>"Si", "scopus_author_id"=>"57189254207"}, {"first_name"=>"Yaeko", "last_name"=>"Nakajima-Takagi", "scopus_author_id"=>"23987186000"}, {"first_name"=>"Kazumasa", "last_name"=>"Aoyama", "scopus_author_id"=>"36097713200"}, {"first_name"=>"Motohiko", "last_name"=>"Oshima", "scopus_author_id"=>"22938579800"}, {"first_name"=>"Atsunori", "last_name"=>"Saraya", "scopus_author_id"=>"8044136100"}, {"first_name"=>"Hiroki", "last_name"=>"Sugishita", "scopus_author_id"=>"57189254134"}, {"first_name"=>"Manabu", "last_name"=>"Nakayama", "scopus_author_id"=>"7401792490"}, {"first_name"=>"Tomoyuki", "last_name"=>"Ishikura", "scopus_author_id"=>"36479993100"}, {"first_name"=>"Haruhiko", "last_name"=>"Koseki", "scopus_author_id"=>"7005219774"}, {"first_name"=>"Atsushi", "last_name"=>"Iwama", "scopus_author_id"=>"35397134700"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"610342465", "doi"=>"10.1371/journal.pone.0154561", "pmid"=>"27136092", "issn"=>"19326203", "scopus"=>"2-s2.0-84966709659", "sgr"=>"84966709659"}, "id"=>"19bcf3b0-02a6-3ad4-8f3e-739376dda29a", "abstract"=>"Polycomb-group RING finger proteins (Pcgf1-Pcgf6) are components of Polycomb repressive complex 1 (PRC1)-related complexes that catalyze monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), an epigenetic mark associated with repression of genes. Pcgf5 has been characterized as a component of PRC1.5, one of the non-canonical PRC1, consisting of Ring1a/b, Rybp/Yaf2 and Auts2. However, the biological functions of Pcgf5 have not yet been identified. Here we analyzed the impact of the deletion of Pcgf5 specifically in hematopoietic stem and progenitor cells (HSPCs). Pcgf5 is expressed preferentially in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) compared with committed myeloid progenitors and differentiated cells. We transplanted bone marrow (BM) cells from Rosa::Cre-ERT control and Cre-ERT;Pcgf5fl/fl mice into lethally irradiated recipient mice. At 4 weeks post-transplantation, we deleted Pcgf5 by injecting tamoxifen, however, no obvious changes in hematopoiesis were detected including the number of HSPCs during a long-term observation period following the deletion. Competitive BM repopulating assays revealed normal repopulating capacity of Pcgf5-deficient HSCs. Nevertheless, Pcgf5-deficient HSPCs showed a significant reduction in H2AK119ub1 levels compared with the control. ChIP-sequence analysis confirmed the reduction in H2AK119ub1 levels, but revealed no significant association of changes in H2AK119ub1 levels with gene expression levels. Our findings demonstrate that Pcgf5-containing PRC1 functions as a histone modifier in vivo, but its role in HSPCs is limited and can be compensated by other PRC1-related complexes in HSPCs.", "link"=>"http://www.mendeley.com/research/loss-pcgf5-affects-global-h2a-monoubiquitination-not-function-hematopoietic-stem-progenitor-cells", "reader_count"=>12, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>3}}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/5042761"], "description"=>"<p>(A) Chimerism of donor-derived cells in recipients in competitive reconstitution assays using the same number of test cells (<i>Cre-ERT</i> and <i>Cre-ERT;Pcgf5</i><sup><i>fl/fl</i></sup>) and competitor cells. After engraftment, <i>Pcgf5</i> was deleted (<i>Pcgf5</i><sup><i>Δ/Δ</i></sup>) by tamoxifen injection. Data are shown as mean ± SD (n = 4–5). (B) Chimerism of donor-derived CD45.2<sup>+</sup> hematopoietic cells in total BM cells, LSK cells, CLPs and myeloid progenitor fractions at 4 months post-transplantation. The data are shown as mean ± SD (n = 7). (C) Chimerism of donor-derived CD45.2<sup>+</sup> cells in splenic LSK cells and donor-derived CD45.2<sup>+</sup> thymocytes in the thymus at 4 months post-transplantation shown as mean ± SD (n = 3). (D, E) Secondary transplantation assays. Total BM cells (5x10<sup>6</sup>) from primary recipient mice at 4 months post-transplantation were transplanted into lethally irradiated secondary recipient mice without competitor cells. Chimerism of donor-derived cells in the PB (D) and total CD45.2<sup>+</sup> hematopoietic cells and LSK cells in the BM (E) at 5 months post-transplantation are shown as mean ± SD (WT, n = 4; <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>, n = 5). *<i>p</i><0.05; n.s., not significant.</p>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212272, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_i_Pcgf5_i_-deficient_HSPCs_retain_normal_reconstitution_capacity_of_hematopoiesis_/3212272", "title"=>"<i>Pcgf5</i>-deficient HSPCs retain normal reconstitution capacity of hematopoiesis.", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-05-02 08:21:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/5042839"], "description"=>"<p>(A) H2AK119ub1 levels in LK cells. LK cells from BM of WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> mice were analyzed by Western blotting using anti-H2AK119ub1 and anti-H3K27me3 antibodies at 1-month post deletion of <i>Pcgf5</i>. Levels of H2AK119ub1 and H3K27me3 were normalized to the amount of H2A and H3, respectively, and are indicated relative to wild-type control values at the bottom. The representative data from two independent experiments are presented. (B) Scatter plots showing the correlation of the fold enrichment values against the input signals (ChIP/input) (transcription start site ± 2.0 kb) of H2AK119ub1 and H3K27me3 of RefSeq genes (listed in RefSeq ID) between WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> GMPs. The light gray lines represent the boundaries for twofold increase and twofold decrease, respectively. The changes of H2AK119ub1 or H3K27me3 levels in genes upregulated and downregulated more than twofold in <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> cells compared with WT cells are indicated in blue and red, respectively. (C) Venn diagram showing the overlap between H2AK119ub1 genes (listed in gene symbol) in GMPs and ES cells. <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> _Down genes are also depicted. (D) Box-and-whisker plots showing H2AK119ub1 and H3K27me3 levels in all Refseq genes (All genes) and H2AK119ub1 genes (genes with ≥ 2-fold enrichment of H2AK119ub1 ChIP signals over the input signals at 2.0 kb ± TSSs in WT GMPs) which showed reduction in H2AK119ub1 levels ≥ 2-fold in <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> GMPs (<i>Pcgf5</i><sup><i>Δ/Δ</i></sup>_Down genes). ***<i>p</i> <0.001 (Student <i>t</i> test). (E) Scatter plots showing the correlation of the fold expression and fold enrichment of H2AK119ub1 ChIP signals in <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> GMPs compared with those in WT GMPs. The genes showing reduction in H2AK119ub1 levels greater than 2-fold (below dotted line) are indicated in red dots. The score of correlation coefficient between the fold expression and fold enrichment of H2AK119ub1 ChIP signals defined with Pearson’s correlation and the linear regression are shown. (F) Box-and-whisker plots showing the expression levels of all RefSeq genes, H2AK119ub1 genes, and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>_Down genes in WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> GMPs in RPKM. Boxes represent 25–75 percentile ranges. Vertical lines represent 10–90 percentile ranges. Horizontal bars represent medians. n.s., not significant. (G) Venn diagram showing the overlap between <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>_Down genes (listed in gene symbol) in GMPs and genes upregulated in expression greater than 2-fold in <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> GMPs relative to WT GMPs. (H) ChIP-sequence data of 3xFlag-Pcgf1, 3xFlag-Pcgf5, and H2AK119ub1 in MEL cells. Venn diagram showing the overlap between Pcgf1 targets, Pcgf5 targets, and H2AK119ub1 genes (≥ 2-fold enrichment of ChIP signals over the input signals at 2.0 kb ± TSSs) (listed in gene symbol) in MEL cells.</p>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212305, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Loss_of_Pcgf5_results_in_reduction_in_global_H2AK119_levels_/3212305", "title"=>"Loss of Pcgf5 results in reduction in global H2AK119 levels.", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-05-02 08:21:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/5042629"], "description"=>"<div><p>Polycomb-group RING finger proteins (Pcgf1-Pcgf6) are components of Polycomb repressive complex 1 (PRC1)-related complexes that catalyze monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), an epigenetic mark associated with repression of genes. Pcgf5 has been characterized as a component of PRC1.5, one of the non-canonical PRC1, consisting of Ring1a/b, Rybp/Yaf2 and Auts2. However, the biological functions of Pcgf5 have not yet been identified. Here we analyzed the impact of the deletion of <i>Pcgf5</i> specifically in hematopoietic stem and progenitor cells (HSPCs). <i>Pcgf5</i> is expressed preferentially in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) compared with committed myeloid progenitors and differentiated cells. We transplanted bone marrow (BM) cells from <i>Rosa</i>::<i>Cre-ERT</i> control and <i>Cre-ERT;Pcgf5</i><sup><i>fl/fl</i></sup> mice into lethally irradiated recipient mice. At 4 weeks post-transplantation, we deleted <i>Pcgf5</i> by injecting tamoxifen, however, no obvious changes in hematopoiesis were detected including the number of HSPCs during a long-term observation period following the deletion. Competitive BM repopulating assays revealed normal repopulating capacity of <i>Pcgf5</i>-deficient HSCs. Nevertheless, <i>Pcgf5</i>-deficient HSPCs showed a significant reduction in H2AK119ub1 levels compared with the control. ChIP-sequence analysis confirmed the reduction in H2AK119ub1 levels, but revealed no significant association of changes in H2AK119ub1 levels with gene expression levels. Our findings demonstrate that Pcgf5-containing PRC1 functions as a histone modifier <i>in vivo</i>, but its role in HSPCs is limited and can be compensated by other PRC1-related complexes in HSPCs.</p></div>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212197, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561", "stats"=>{"downloads"=>2, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Loss_of_Pcgf5_Affects_Global_H2A_Monoubiquitination_but_Not_the_Function_of_Hematopoietic_Stem_and_Progenitor_Cells/3212197", "title"=>"Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells", "pos_in_sequence"=>1, "defined_type"=>3, "published_date"=>"2016-05-02 08:21:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/5042710"], "description"=>"<p>(A) PB cell counts of recipients repopulated with <i>Cre-ERT</i> (+/+) and <i>Cre-ERT;Pcgf5</i><sup><i>fl/fl</i></sup> BM cells after deletion of <i>Pcgf5</i> (<i>Δ/Δ</i>) by tamoxifen injection. Data are shown as mean ± SD (n = 4–5). (B) Lineage contribution of donor cells to myeloid (Gr-1<sup>+</sup> and/or Mac-1<sup>+</sup>), B (B220<sup>+</sup>), or T (CD4<sup>+</sup> and/or CD8<sup>+</sup>) cells in the PB shown as mean ± SD (n = 4–5). (C) Absolute number of CD45.2<sup>+</sup> donor-derived hematopoietic cells in a unilateral pair of femur and tibia of recipients at 5 months post-transplantation. Data are shown as mean ± SD (WT, n = 5; <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>, n = 6). (D) Absolute number of CD45.2<sup>+</sup> donor-derived LSK cells, CLPs and myeloid progenitors in the BM of recipient mice at 5 months post-transplantation presented as mean ± SD (WT, n = 5; <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>, n = 6). n.s., not significant.</p>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212251, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Depletion_of_i_Pcgf5_i_does_not_compromise_adult_hematopoiesis_/3212251", "title"=>"Depletion of <i>Pcgf5</i> does not compromise adult hematopoiesis.", "pos_in_sequence"=>3, "defined_type"=>1, "published_date"=>"2016-05-02 08:21:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/5042653"], "description"=>"<p>(A) RT-PCR analysis of <i>Pcgf5</i> in BM hematopoietic cell fractions. Cells analyzed were CD34<sup>-</sup>LSK long-term HSCs, CD34<sup>+</sup>Flt3<sup>-</sup>LSK short-term HSCs, CD34<sup>+</sup>Flt3<sup>+</sup>LSK multipotent progenitors (MPPs), common myeloid progenitors (CMPs), granulocyte-macrophage progenitors (GMPs), megakaryocyte-erythroid progenitors (MEPs), and lineage marker<sup>+</sup> mature hematopoietic cells. <i>Hypoxanthine-guanosine phosphoribosyl transferase (Hprt)</i> was used as a housekeeping control gene. Data are shown as the mean ± standard deviation (SD) for triplicate analyses. (B) Strategy for making a conditional knockout allele for <i>Pcgf5</i> by homologous recombination in ES cells. FRT recombinase was used to remove the <i>Neo</i> cassette. (C) Scheme of the hematopoietic repopulation assay. Total BM cells (5x10<sup>6</sup> cells) from <i>Cre-ERT</i> and <i>Cre-ERT;Pcgf5</i><sup><i>fl/fl</i></sup> were transplanted into lethally irradiated CD45.1 recipient mice without competitor BM cells, or 2x10<sup>6</sup> total BM cells were transplanted with the same number of competitor BM cells. To induce deletion of <i>Pcgf5</i>, 100 μl of tamoxifen (10 mg/ml) was intraperitoneally injected once a day for five consecutive days at 1 month post-transplantation. (D) Efficient deletion of <i>Pcgf5</i> in hematopoietic cells detected by genomic PCR. Deletion of <i>Pcgf5</i> in <i>Cre-ERT;Pcgf5</i><sup><i>fl/fl</i></sup> PB myeloid cells was evaluated pre- and post-tamoxifen treatment. “WT”, “Floxed”, and “<i>Δ</i>” alleles indicate the wild-type and floxed <i>Pcgf5</i> allele, and floxed <i>Pcgf5</i> allele after removal of exon 2 by Cre recombinase, respectively. (E) Detection of truncated <i>Pcgf5</i> mRNA in BM <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> LK cells using primers directed to exon 1 and exon 5/6 junction. (F) Pcgf5 interacts with Ring1B. Ring1B in lysates from <i>Pcgf5</i><sup><i>fl/fl</i></sup> and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> ES cells was immunoprecipitated using anti-Ring1b antibody, and then immunoprecipitates were detected by immunoblotting using anti-Ring1b and anti-Pcgf5 antibodies.</p>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212221, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Generation_of_conditional_knockout_allele_for_i_Pcgf5_i_in_mice_/3212221", "title"=>"Generation of conditional knockout allele for <i>Pcgf5</i> in mice.", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-05-02 08:21:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/5042809"], "description"=>"<p>(A) Snapshots of RNA-sequence signals at the <i>Pcgf5</i> gene locus in WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> LSK cells isolated from recipient mice repopulated with <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> hematopoietic cells. The structure of <i>Pcgf5</i> gene locus including relevant exons is indicated at the bottom. (B) Scatter diagrams showing RNA-sequence data of LSK cells and GMPs. Expression levels of RefSeq genes (listed in RefSeq ID) defined by reads per kilobase of exon per million fragments mapped (RPKM) in log2 in WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> cells are plotted. The light gray lines represent the boundaries for twofold increase and twofold decrease, respectively. The number of genes upregulated and downregulated more than twofold in <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> cells compared with WT cells are indicated in red and blue, respectively. (C) Box-and-whisker plots showing the expression levels of WT and <i>Pcgf5</i><sup><i>Δ/Δ</i></sup> LSK cells and GMPs in RPKM. Boxes represent 25–75 percentile ranges. Vertical lines represent 10–90 percentile ranges. Horizontal bars represent medians. n.s., not significant. (D) Venn diagram showing the overlap between genes (listed in gene symbol) up-regulated in LSK cells from <i>Pcgf5</i><sup><i>Δ/Δ</i></sup>, <i>Ezh2</i><sup><i>Δ/Δ</i></sup> [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154561#pone.0154561.ref018\" target=\"_blank\">18</a>], and <i>Bmi1</i> KO [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154561#pone.0154561.ref019\" target=\"_blank\">19</a>] mice (≥2.0-fold compared with the WT control, respectively). The numbers of genes in each group are indicated. The statistical significance of the overlaps between the two gene groups is indicated. (E) Expression of <i>Pcgf</i> genes in WT LSK cells and GMPs in RPKM.</p>", "links"=>[], "tags"=>["histone H 2A", "PRC", "H 2AK levels", "HSPC", "HSC", "MPP", "Pcgf 5 Affects Global H 2A Monoubiquitination", "Competitive BM repopulating assays", "Pcgf 5", "gene expression levels"], "article_id"=>3212287, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Molecular Biology", "Physiology", "Biological Sciences not elsewhere classified", "Developmental Biology", "Cancer", "Hematology"], "users"=>["Sha Si", "Yaeko Nakajima-Takagi", "Kazumasa Aoyama", "Motohiko Oshima", "Atsunori Saraya", "Hiroki Sugishita", "Manabu Nakayama", "Tomoyuki Ishikura", "Haruhiko Koseki", "Atsushi Iwama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0154561.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Gene_expression_profile_of_i_Pcgf5_i_-deficient_HSPCs_/3212287", "title"=>"Gene expression profile of <i>Pcgf5</i>-deficient HSPCs.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-05-02 08:21:54"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
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