The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease
Publication Date
May 11, 2016
Journal
PLOS ONE
Authors
Tathiana Ferreira Sá Antunes, Raquel J. Vionette Amaral, José Aires Ventura, Marcio Tadeu Godinho, et al
Volume
11
Issue
5
Pages
e0155240
DOI
https://dx.plos.org/10.1371/journal.pone.0155240
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0155240
Scopus
84969759738
Mendeley
http://www.mendeley.com/research/dsrna-virus-papaya-meleira-virus-ssrna-virus-associated-papaya-sticky-disease
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The dsRNA virus papaya meleira virus and an ssRNA virus are associated with papaya sticky disease", "type"=>"journal", "authors"=>[{"first_name"=>"Tathiana Ferreira Sá", "last_name"=>"Antunes", "scopus_author_id"=>"54079352900"}, {"first_name"=>"Raquel J.Vionette", "last_name"=>"Amaral", "scopus_author_id"=>"57189372408"}, {"first_name"=>"José Aires", "last_name"=>"Ventura", "scopus_author_id"=>"7102389059"}, {"first_name"=>"Marcio Tadeu", "last_name"=>"Godinho", "scopus_author_id"=>"57189373812"}, {"first_name"=>"Josiane G.", "last_name"=>"Amaral", "scopus_author_id"=>"57189359507"}, {"first_name"=>"Flávia O.", "last_name"=>"Souza", "scopus_author_id"=>"57189372400"}, {"first_name"=>"Poliane Alfenas", "last_name"=>"Zerbini", "scopus_author_id"=>"57189364955"}, {"first_name"=>"Francisco Murilo", "last_name"=>"Zerbini", "scopus_author_id"=>"6603035678"}, {"first_name"=>"Patricia Machado Bueno", "last_name"=>"Fernandes", "scopus_author_id"=>"7102795076"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pmid"=>"27166626", "pui"=>"610397773", "scopus"=>"2-s2.0-84969759738", "doi"=>"10.1371/journal.pone.0155240", "sgr"=>"84969759738"}, "id"=>"4b860ea7-5e7f-34c6-9654-ae0cbe6f4896", "abstract"=>"Papaya sticky disease, or \"meleira\", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.", "link"=>"http://www.mendeley.com/research/dsrna-virus-papaya-meleira-virus-ssrna-virus-associated-papaya-sticky-disease", "reader_count"=>13, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>9}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84969759738"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84969759738?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84969759738&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84969759738&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84969759738", "dc:identifier"=>"SCOPUS_ID:84969759738", "eid"=>"2-s2.0-84969759738", "dc:title"=>"The dsRNA virus papaya meleira virus and an ssRNA virus are associated with papaya sticky disease", "dc:creator"=>"Antunes T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"11", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2016-05-01", "prism:coverDisplayDate"=>"1 May 2016", "prism:doi"=>"10.1371/journal.pone.0155240", "citedby-count"=>"8", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Federal University of Espirito Santo", "affiliation-city"=>"Vitória", "affiliation-country"=>"Brazil"}], "pubmed-id"=>"27166626", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0155240", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0155240", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"60", "xml_views"=>"0", "html_views"=>"139"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"5", "html_views"=>"58"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"8", "html_views"=>"56"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"55"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"168"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"224"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/5224810"], "description"=>"<p>The viral ssRNA contains two ORFs. ORF1 encodes a hypothetical protein and ORF2 encodes a putative RdRp.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379585, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Schematic_representation_of_the_genomic_organization_of_papaya_umbraviruspapaya_meleira_virus_2_PpUVPMeV2_/3379585", "title"=>"Schematic representation of the genomic organization of papaya umbraviruspapaya meleira virus 2 (PpUVPMeV2).", "pos_in_sequence"=>4, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224855"], "description"=>"<p>(a) Viral bands after centrifugation in a sucrose density gradient. T, top; M, medium. B, bottom. (b) Transmission electron microscope images of viral particles from the T, M and B fractions, as indicated. Images T and M, 140,000x. Image B, 85,000x. (c) Agarose gel electrophoresis of RNA extracted from particles in the M and B fractions, as indicated. No RNAs were obtained from particles in the T fraction. The left lane is a size marker [1 kb plus DNA ladder (Invitrogen), in kbp].</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379618, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Analysis_of_purified_viral_preparations_from_the_latex_of_papaya_plants_displaying_severe_symptoms_of_sticky_disease_/3379618", "title"=>"Analysis of purified viral preparations from the latex of papaya plants displaying severe symptoms of sticky disease.", "pos_in_sequence"=>7, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224864"], "description"=>"<p>(a) List of the nine peptides identified by mass spectrometry, including their MASCOT scores and their coordinates (start and end) within the ORF1 aa sequence. (b) Deduced amino acid sequence of the central portion of ORF1, highlighting the positions of the nine peptides listed in (a). Note that all nine peptides display 100% identity with the ORF1 aa sequence. The complete amino acid sequence of PeMV ORF1 is 1563 aa long.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379624, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Peptide_mapping_of_the_PMeV_PpUVPMeV2_structural_CP_protein_in_the_deduced_amino_acid_aa_sequence_of_PMeV_ORF1_/3379624", "title"=>"Peptide mapping of the PMeV/PpUVPMeV2 structural (CP) protein in the deduced amino acid (aa) sequence of PMeV ORF1.", "pos_in_sequence"=>8, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224786"], "description"=>"<p>(a) Schematic representation of the PMeV genomic organization. The viral dsRNA contains two ORFs. ORF1 encodes the putative coat protein (CP) and ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp). The position of a putative slippery sequence for -1 translational frameshift is indicated at the 3'-end of ORF1. (b) Predicted pseudoknot structure located downstream of the slippery sequence.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379561, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Genome_organization_and_molecular_features_of_papaya_meleira_virus_PMeV_/3379561", "title"=>"Genome organization and molecular features of papaya meleira virus (PMeV).", "pos_in_sequence"=>2, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224828"], "description"=>"<p>Phylogenetic tree based on an alignment of deduced amino acid sequences of the RdRp proteins from PMeV and members of the family <i>Totiviridae</i>, obtained using Bayesian inference. The numbers at the branch nodes indicate Bayesian posterior probabilities. The names of the viruses used in the analysis and their respective GenBank access numbers are listed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155240#pone.0155240.s003\" target=\"_blank\">S2 Table</a>.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379597, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Phylogenetic_relationship_between_PMeV_and_related_viruses_/3379597", "title"=>"Phylogenetic relationship between PMeV and related viruses.", "pos_in_sequence"=>5, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224795"], "description"=>"<p>Alignment of the RdRp4 domain (pfam02123) identified in ORF2 of papaya meleira virus (PMeV) with the corresponding regions of the most closely related totivirid-like viruses (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155240#pone.0155240.s003\" target=\"_blank\">S2 Table</a> for full virus names and GenBank access numbers). Sequences were aligned with MUSCLE (Edgar, 2004). The numbers in parenthesis indicate the number of amino acid residues separating individual motifs. Amino acid residues highlighted in black are conserved among all aligned sequences.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379570, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Alignment_of_the_RdRp4_domain_from_PMeV_and_related_viruses_/3379570", "title"=>"Alignment of the RdRp4 domain from PMeV and related viruses.", "pos_in_sequence"=>3, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224843"], "description"=>"<p>Phylogenetic tree based on an alignment of the deduced amino acid sequences of PpUVPMeV2 and members of the family <i>Tombusviridae</i>, obtained using Bayesian inference. The numbers at the branch nodes indicate Bayesian posterior probabilities. The names of the viruses used in the analysis and their respective GenBank access numbers are listed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155240#pone.0155240.s003\" target=\"_blank\">S2 Table</a>.</p>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379609, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0155240.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Phylogenetic_relationship_between_PpUVPMeV2_and_related_viruses_/3379609", "title"=>"Phylogenetic relationship between PpUVPMeV2 and related viruses.", "pos_in_sequence"=>6, "defined_type"=>1, "published_date"=>"2016-05-11 05:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/5224753", "https://ndownloader.figshare.com/files/5224765", "https://ndownloader.figshare.com/files/5224768"], "description"=>"<div><p>Papaya sticky disease, or “meleira”, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family <i>Totiviridae</i>. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.</p></div>", "links"=>[], "tags"=>["mass spectrometry analysis", "PMeV ORF 1.", "ORF 2 display similarity", "papaya meleira virus", "dsRNA Virus Papaya Meleira Virus", "papaya virus Q", "PMeV dsRNA", "Papaya Sticky Disease Papaya"], "article_id"=>3379546, "categories"=>["Biochemistry", "Microbiology", "Genetics", "Molecular Biology", "Biotechnology", "Evolutionary Biology", "Chemical Sciences not elsewhere classified", "Immunology", "Biological Sciences not elsewhere classified", "Cancer", "Infectious Diseases", "Plant Biology", "Virology"], "users"=>["Tathiana Ferreira Sá Antunes", "Raquel J. Vionette Amaral", "José Aires Ventura", "Marcio Tadeu Godinho", "Josiane G. Amaral", "Flávia O. Souza", "Poliane Alfenas Zerbini", "Francisco Murilo Zerbini", "Patricia Machado Bueno Fernandes"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0155240.s001", "https://dx.doi.org/10.1371/journal.pone.0155240.s002", "https://dx.doi.org/10.1371/journal.pone.0155240.s003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_dsRNA_Virus_Papaya_Meleira_Virus_and_an_ssRNA_Virus_Are_Associated_with_Papaya_Sticky_Disease/3379546", "title"=>"The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease", "pos_in_sequence"=>1, "defined_type"=>4, "published_date"=>"2016-05-11 05:58:35"}

PMC Usage Stats

  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"31", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"24", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"32", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"25", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"46", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2016-01-01T00:00:00Z", "end_date"=>"2016-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts