Modification of Cry4Aa toward Improved Toxin Processing in the Gut of the Pea Aphid, Acyrthosiphon pisum
Publication Date
May 12, 2016
Journal
PLOS ONE
Authors
Michael A. Rausch, Nanasaheb P. Chougule, Benjamin R. Deist & Bryony C. Bonning
Volume
11
Issue
5
Pages
e0155466
DOI
https://dx.plos.org/10.1371/journal.pone.0155466
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0155466
Web of Science
000376588600086
Scopus
84970028206
Mendeley
http://www.mendeley.com/research/modification-cry4aa-toward-improved-toxin-processing-gut-pea-aphid-acyrthosiphon-pisum
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Mendeley | Further Information

{"title"=>"Modification of Cry4Aa toward improved toxin processing in the gut of the pea aphid, Acyrthosiphon pisum", "type"=>"journal", "authors"=>[{"first_name"=>"Michael A.", "last_name"=>"Rausch", "scopus_author_id"=>"54393964200"}, {"first_name"=>"Nanasaheb P.", "last_name"=>"Chougule", "scopus_author_id"=>"6507956630"}, {"first_name"=>"Benjamin R.", "last_name"=>"Deist", "scopus_author_id"=>"56703495300"}, {"first_name"=>"Bryony C.", "last_name"=>"Bonning", "scopus_author_id"=>"57195255260"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84970028206", "pui"=>"610397838", "doi"=>"10.1371/journal.pone.0155466", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84970028206", "pmid"=>"27171411"}, "id"=>"1502cfa7-65ee-31fa-b21c-9c8b3e0a81ca", "abstract"=>"Aphids are sap-sucking insects (order: Hemiptera) that cause extensive damage to a wide range of agricultural crops. Our goal was to optimize a naturally occurring insecticidal crystalline (Cry) toxins produced by the soil-dwelling bacterium Bacillus thuringiensis for use against the pea aphid, Acyrthosiphon pisum. On the basis that activation of the Cry4Aa toxin is a rate-limiting factor contributing to the relatively low aphicidal activity of this toxin, we introduced cathepsin L and cathepsin B cleavage sites into Cry4Aa for rapid activation in the aphid gut environment. Incubation of modified Cry4Aa and aphid proteases in vitro demonstrated enhanced processing of the toxin into the active form for some of the modified constructs relative to non-modified Cry4Aa. Aphids fed artificial diet with toxin at a final concentration of 125 mug/ml showed enhanced mortality after two days for one of the four modified constructs. Although only modest toxin improvement was achieved by use of this strategy, such specific toxin modifications designed to overcome factors that limit aphid toxicity could be applied toward managing aphid populations via transgenic plant resistance.", "link"=>"http://www.mendeley.com/research/modification-cry4aa-toward-improved-toxin-processing-gut-pea-aphid-acyrthosiphon-pisum", "reader_count"=>12, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>3, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>3, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>10}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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