Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein
Publication Date
June 03, 2016
Journal
PLOS ONE
Authors
Zhifeng Chen, Lan Zhang, Aimin Tang, Cheryl Callahan, et al
Volume
11
Issue
6
Pages
e0156798
DOI
https://dx.plos.org/10.1371/journal.pone.0156798
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0156798
Web of Science
000377369700131
Scopus
84974827212
Mendeley
http://www.mendeley.com/research/discovery-characterization-phage-displayderived-human-monoclonal-antibodies-against-rsv-f-glycoprote
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Mendeley | Further Information

{"title"=>"Discovery and characterization of phage display-derived human monoclonal antibodies against RSV F glycoprotein", "type"=>"journal", "authors"=>[{"first_name"=>"Zhifeng", "last_name"=>"Chen", "scopus_author_id"=>"57188979143"}, {"first_name"=>"Lan", "last_name"=>"Zhang", "scopus_author_id"=>"55200700300"}, {"first_name"=>"Aimin", "last_name"=>"Tang", "scopus_author_id"=>"36007608400"}, {"first_name"=>"Cheryl", "last_name"=>"Callahan", "scopus_author_id"=>"36462436100"}, {"first_name"=>"Pavlo", "last_name"=>"Pristatsky", "scopus_author_id"=>"6507226613"}, {"first_name"=>"Ryan", "last_name"=>"Swoyer", "scopus_author_id"=>"26868152800"}, {"first_name"=>"Pedro", "last_name"=>"Cejas", "scopus_author_id"=>"57189712356"}, {"first_name"=>"Debbie", "last_name"=>"Nahas", "scopus_author_id"=>"6602378170"}, {"first_name"=>"Jennifer", "last_name"=>"Galli", "scopus_author_id"=>"56025669000"}, {"first_name"=>"Scott", "last_name"=>"Cosmi", "scopus_author_id"=>"57189715531"}, {"first_name"=>"Daniel", "last_name"=>"DiStefano", "scopus_author_id"=>"6602180111"}, {"first_name"=>"Van M.", "last_name"=>"Hoang", "scopus_author_id"=>"56830202100"}, {"first_name"=>"Andrew", "last_name"=>"Bett", "scopus_author_id"=>"7005920246"}, {"first_name"=>"Danilo", "last_name"=>"Casimiro", "scopus_author_id"=>"6701824527"}, {"first_name"=>"Kalpit A.", "last_name"=>"Vora", "scopus_author_id"=>"7004421047"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27258388", "doi"=>"10.1371/journal.pone.0156798", "issn"=>"19326203", "scopus"=>"2-s2.0-84974827212", "pui"=>"610615695", "sgr"=>"84974827212"}, "id"=>"a9811d08-8b14-3b89-a4fd-6003d998bcd4", "abstract"=>"Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infection in infants, the elderly and in immunosuppressed populations. The vast majority of neutralizing antibodies isolated from human subjects target the RSV fusion (F) glycoprotein, making it an attractive target for the development of vaccines and therapeutic antibodies. Currently, Synagis (palivizumab) is the only FDA approved antibody drug for the prevention of RSV infection, and there is a great need for more effective vaccines and therapeutics. Phage display is a powerful tool in antibody discovery with the advantage that it does not require samples from immunized subjects. In this study, Morphosys HuCAL GOLD phage libraries were used for panning against RSV prefusion and postfusion F proteins. Panels of human monoclonal antibodies (mAbs) against RSV F protein were discovered following phage library panning and characterized. Antibodies binding specifically to prefusion or postfusion F proteins and those binding both conformations were identified. 3B1 is a prototypic postfusion F specific antibody while 2E1 is a prototypic prefusion F specific antibody. 2E1 is a potent broadly neutralizing antibody against both RSV A and B strains. Epitope mapping experiments identified a conformational epitope spanning across three discontinuous sections of the RSV F protein, as well as critical residues for antibody interaction.", "link"=>"http://www.mendeley.com/research/discovery-characterization-phage-displayderived-human-monoclonal-antibodies-against-rsv-f-glycoprote", "reader_count"=>17, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>3, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>3, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "group_count"=>1}

Scopus | Further Information

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