Evaluation of Nucleic Acid Preservation Cards for West Nile Virus Testing in Dead Birds
Publication Date
June 24, 2016
Journal
PLOS ONE
Authors
Leslie Foss, William K. Reisen, Ying Fang, Vicki Kramer, et al
Volume
11
Issue
6
Pages
e0157555
DOI
https://dx.plos.org/10.1371/journal.pone.0157555
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0157555
Scopus
84976509454
Mendeley
http://www.mendeley.com/research/evaluation-nucleic-acid-preservation-cards-west-nile-virus-testing-dead-birds
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Mendeley | Further Information

{"title"=>"Evaluation of nucleic acid preservation cards for West Nile virus testing in dead birds", "type"=>"journal", "authors"=>[{"first_name"=>"Leslie", "last_name"=>"Foss", "scopus_author_id"=>"55755539500"}, {"first_name"=>"William K.", "last_name"=>"Reisen", "scopus_author_id"=>"7004966946"}, {"first_name"=>"Ying", "last_name"=>"Fang", "scopus_author_id"=>"55469320500"}, {"first_name"=>"Vicki", "last_name"=>"Kramer", "scopus_author_id"=>"7004447843"}, {"first_name"=>"Kerry", "last_name"=>"Padgett", "scopus_author_id"=>"6701581845"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84976509454", "doi"=>"10.1371/journal.pone.0157555", "isbn"=>"1932-6203", "pmid"=>"27341492", "issn"=>"19326203", "scopus"=>"2-s2.0-84976509454", "pui"=>"610977563"}, "id"=>"85aa9945-dd00-3818-a98b-9944a48d6b6d", "abstract"=>"The California West Nile virus (WNV) Dead Bird Surveillance Program (DBSP) is an important component of WNV surveillance in the state. We evaluated FTA™ and RNASound™ cards as an alternative method for sampling dead birds for WNV molecular testing as these cards allow for more cost effective, rapid, and safer diagnostic sampling than the shipment of bird carcasses. To evaluate accuracy of results among avian sampling regimes, Reverse-Transcription Polymerase Chain Reaction (RT-PCR) results from FTA™ and RNASound™ cards were compared with results from kidney tissue, brain tissue, or oral swabs in lysis buffer in 2012-2013. In addition, RT-PCR results were compared with results from oral swabs tested by rapid antigen tests (RAMP™ and VecTOR™). While test results from the cards were not as sensitive as kidney tissue testing, they were more likely to provide accurate results than rapid antigen tests, and detected WNV in corvids as well as in other passerines, raptors, and waterfowl. Overall, WNV RT-PCR cycle threshold (Ct) scores from the cards were higher than those from tissue testing, but both card products displayed high sensitivity and specificity. American Crow samples provided the highest sensitivity. The cards also proved to be easier and more convenient vehicles for collecting and shipping samples, and in 2014 our program launched use of RNASound™ cards in the DBSP. Both FTA™ and RNASound™ products displayed 96% agreement with tissue results and are an adequate alternative sampling method for WNV dead bird testing.", "link"=>"http://www.mendeley.com/research/evaluation-nucleic-acid-preservation-cards-west-nile-virus-testing-dead-birds", "reader_count"=>8, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Ph. D. Student"=>3, "Other"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Ph. D. Student"=>3, "Other"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1, "Veterinary Science and Veterinary Medicine"=>2, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>2}}, "group_count"=>1}

Scopus | Further Information

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