The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture
Publication Date
June 23, 2016
Journal
PLOS ONE
Authors
Lenka Tesarova, Pavel Simara, Stanislav Stejskal & Irena Koutna
Volume
11
Issue
6
Pages
e0157974
DOI
https://dx.plos.org/10.1371/journal.pone.0157974
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0157974
Scopus
84976543944
Mendeley
http://www.mendeley.com/research/aberrant-dna-methylation-profile-human-induced-pluripotent-stem-cells-connected-reprogramming-proces
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Mendeley | Further Information

{"title"=>"The aberrant DNA methylation profile of human induced pluripotent stem cells is connected to the reprogramming process and is normalized during in vitro culture", "type"=>"journal", "authors"=>[{"first_name"=>"Lenka", "last_name"=>"Tesarova", "scopus_author_id"=>"34868823200"}, {"first_name"=>"Pavel", "last_name"=>"Simara", "scopus_author_id"=>"26538192200"}, {"first_name"=>"Stanislav", "last_name"=>"Stejskal", "scopus_author_id"=>"23398607600"}, {"first_name"=>"Irena", "last_name"=>"Koutna", "scopus_author_id"=>"6602503402"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27336948", "doi"=>"10.1371/journal.pone.0157974", "pui"=>"610977430", "issn"=>"19326203", "sgr"=>"84976543944", "scopus"=>"2-s2.0-84976543944"}, "id"=>"476766b6-17c3-3d4f-8c36-ff1dedd62deb", "abstract"=>"The potential clinical applications of human induced pluripotent stem cells (hiPSCs) are limited by genetic and epigenetic variations among hiPSC lines and the question of their equivalency with human embryonic stem cells (hESCs). We used MethylScreen technology to determine the DNA methylation profile of pluripotency and differentiation markers in hiPSC lines from different source cell types compared to hESCs and hiPSC source cells. After derivation, hiPSC lines compromised a heterogeneous population characterized by variable levels of aberrant DNA methylation. These aberrations were induced during somatic cell reprogramming and their levels were associated with the type of hiPSC source cells. hiPSC population heterogeneity was reduced during prolonged culture and hiPSCs acquired an hESC-like methylation profile. In contrast, the expression of differentiation marker genes in hiPSC lines remained distinguishable from that in hESCs. Taken together, in vitro culture facilitates hiPSC acquisition of hESC epigenetic characteristics. However, differences remain between both pluripotent stem cell types, which must be considered before their use in downstream applications.", "link"=>"http://www.mendeley.com/research/aberrant-dna-methylation-profile-human-induced-pluripotent-stem-cells-connected-reprogramming-proces", "reader_count"=>12, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>5}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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