A Multi-Species TaqMan PCR Assay for the Identification of Asian Gypsy Moths (Lymantria spp.) and Other Invasive Lymantriines of Biosecurity Concern to North America
Publication Date
August 11, 2016
Journal
PLOS ONE
Authors
Donald Stewart, Reza Zahiri, Abdelmadjid Djoumad, Luca Freschi, et al
Volume
11
Issue
8
Pages
e0160878
DOI
https://dx.plos.org/10.1371/journal.pone.0160878
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0160878
Scopus
84983535424
Mendeley
http://www.mendeley.com/research/multispecies-taqman-pcr-assay-identification-asian-gypsy-moths-lymantria-spp-other-invasive-lymantri
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Mendeley | Further Information

{"title"=>"A multi-species TaqMan PCR assay for the identification of asian gypsy moths (Lymantria spp.) and other invasive lymantriines of biosecurity concern to North America", "type"=>"journal", "authors"=>[{"first_name"=>"Donald", "last_name"=>"Stewart", "scopus_author_id"=>"15130240000"}, {"first_name"=>"Reza", "last_name"=>"Zahiri", "scopus_author_id"=>"15840999600"}, {"first_name"=>"Abdelmadjid", "last_name"=>"Djoumad", "scopus_author_id"=>"14318919800"}, {"first_name"=>"Luca", "last_name"=>"Freschi", "scopus_author_id"=>"38061217200"}, {"first_name"=>"Josyanne", "last_name"=>"Lamarche", "scopus_author_id"=>"7005572556"}, {"first_name"=>"Dave", "last_name"=>"Holden", "scopus_author_id"=>"36881151700"}, {"first_name"=>"Sandra", "last_name"=>"Cervantes", "scopus_author_id"=>"57190859476"}, {"first_name"=>"Dario I.", "last_name"=>"Ojeda", "scopus_author_id"=>"55758895700"}, {"first_name"=>"Am�lie", "last_name"=>"Potvin", "scopus_author_id"=>"57201107346"}, {"first_name"=>"Audrey", "last_name"=>"Nisole", "scopus_author_id"=>"23061527400"}, {"first_name"=>"Catherine", "last_name"=>"B�liveau", "scopus_author_id"=>"57201102540"}, {"first_name"=>"Arnaud", "last_name"=>"Capron", "scopus_author_id"=>"7202272911"}, {"first_name"=>"Troy", "last_name"=>"Kimoto", "scopus_author_id"=>"56028373900"}, {"first_name"=>"Brittany", "last_name"=>"Day", "scopus_author_id"=>"57190864355"}, {"first_name"=>"Hesther", "last_name"=>"Yueh", "scopus_author_id"=>"12799877100"}, {"first_name"=>"Cameron", "last_name"=>"Duff", "scopus_author_id"=>"57190485824"}, {"first_name"=>"Roger C.", "last_name"=>"Levesque", "scopus_author_id"=>"7006275837"}, {"first_name"=>"Richard C.", "last_name"=>"Hamelin", "scopus_author_id"=>"7006877253"}, {"first_name"=>"Michel", "last_name"=>"Cusson", "scopus_author_id"=>"7004536952"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84983535424", "doi"=>"10.1371/journal.pone.0160878", "sgr"=>"84983535424", "issn"=>"19326203", "pui"=>"611775444"}, "id"=>"8205c919-fea8-3873-b844-a1f43bb4cb57", "abstract"=>"Preventing the introduction and establishment of forest invasive alien species (FIAS) such as the Asian gypsy moth (AGM) is a high-priority goal for countries with extensive forest resources such as Canada. The name AGM designates a group of closely related Lymantria species (Lepidoptera: Erebidae: Lymantriinae) comprising two L. dispar subspecies (L. dispar asiatica, L. dispar japonica) and three closely related Lymantria species (L. umbrosa, L. albescens, L. postalba), all considered potential FIAS in North America. Ships entering Canadian ports are inspected for the presence of suspicious gypsy moth eggs, but those of AGM are impossible to distinguish from eggs of innocuous Lymantria species. To assist regulatory agencies in their identification of these insects, we designed a suite of TaqMan1 assays that provide significant improvements over existing molecular assays targeting AGM. The assays presented here can identify all three L. dispar subspecies (including the European gypsy moth, L. dispar dispar), the three other Lymantria species comprising the AGM complex, plus five additional Lymantria species that pose a threat to forests in North America. The suite of assays is built as a “molecular key” (analogous to a taxonomic key) and involves several parallel singleplex and multiplex qPCR reactions. Each reaction uses a combination of primers and probes designed to separate taxa through discriminatory annealing. The success of these assays is based on the presence of single nucleotide polymorphisms (SNPs) in the 5’ region of mitochondrial cytochrome c oxidase I (COI) or in its longer, 3’ region, as well as on the presence of an indel in the “FS1” nuclear marker,", "link"=>"http://www.mendeley.com/research/multispecies-taqman-pcr-assay-identification-asian-gypsy-moths-lymantria-spp-other-invasive-lymantri", "reader_count"=>10, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>6}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>2}}, "group_count"=>0}

Scopus | Further Information

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