Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume
Publication Date
September 06, 2016
Journal
PLOS ONE
Authors
Brian L. Zielinski, Andrew E. Allen, Edward J. Carpenter, Victoria J. Coles, et al
Volume
11
Issue
9
Pages
e0160929
DOI
https://dx.plos.org/10.1371/journal.pone.0160929
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0160929
Scopus
84991661948
Mendeley
http://www.mendeley.com/research/patterns-transcript-abundance-eukaryotic-biogeochemicallyrelevant-genes-amazon-river-plume
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Mendeley | Further Information

{"title"=>"Patterns of transcript abundance of eukaryotic biogeochemically-relevant genes in the Amazon river plume", "type"=>"journal", "authors"=>[{"first_name"=>"Brian L.", "last_name"=>"Zielinski", "scopus_author_id"=>"35104219200"}, {"first_name"=>"Andrew E.", "last_name"=>"Allen", "scopus_author_id"=>"7402802528"}, {"first_name"=>"Edward J.", "last_name"=>"Carpenter", "scopus_author_id"=>"7101893116"}, {"first_name"=>"Victoria J.", "last_name"=>"Coles", "scopus_author_id"=>"6603388837"}, {"first_name"=>"Byron C.", "last_name"=>"Crump", "scopus_author_id"=>"7003547549"}, {"first_name"=>"Mary", "last_name"=>"Doherty", "scopus_author_id"=>"36127401100"}, {"first_name"=>"Rachel A.", "last_name"=>"Foster", "scopus_author_id"=>"12791687400"}, {"first_name"=>"Joaquim I.", "last_name"=>"Goes", "scopus_author_id"=>"7005857264"}, {"first_name"=>"Helga R.", "last_name"=>"Gomes", "scopus_author_id"=>"56659171400"}, {"first_name"=>"Raleigh R.", "last_name"=>"Hood", "scopus_author_id"=>"7102962473"}, {"first_name"=>"John P.", "last_name"=>"McCrow", "scopus_author_id"=>"36028518900"}, {"first_name"=>"Joseph P.", "last_name"=>"Montoya", "scopus_author_id"=>"7101654856"}, {"first_name"=>"Ahmed", "last_name"=>"Moustafa", "scopus_author_id"=>"55251149600"}, {"first_name"=>"Brandon M.", "last_name"=>"Satinsky", "scopus_author_id"=>"55344610700"}, {"first_name"=>"Shalabh", "last_name"=>"Sharma", "scopus_author_id"=>"56140105300"}, {"first_name"=>"Christa B.", "last_name"=>"Smith", "scopus_author_id"=>"55570865300"}, {"first_name"=>"Patricia L.", "last_name"=>"Yager", "scopus_author_id"=>"7005537246"}, {"first_name"=>"John H.", "last_name"=>"Paul", "scopus_author_id"=>"8836527800"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pmid"=>"27598790", "scopus"=>"2-s2.0-84991661948", "doi"=>"10.1371/journal.pone.0160929", "sgr"=>"84991661948", "pui"=>"612703571"}, "id"=>"ed1fd17a-df29-304a-84ca-8c5135ee0ee2", "abstract"=>"The Amazon River has the largest discharge of all rivers on Earth, and its complex plume system fuels a wide array of biogeochemical processes, across a large area of the western tropical North Atlantic. The plume thus stimulates microbial processes affecting carbon sequestration and nutrient cycles at a global scale. Chromosomal gene expression patterns of the 2.0 to 156 μm size-fraction eukaryotic microbial community were investigated in the Amazon River Plume, generating a robust dataset (more than 100 million mRNA sequences) that depicts the metabolic capabilities and interactions among the eukaryotic microbes. Combining classical oceanographic field measurements with metatranscriptomics yielded characterization of the hydrographic conditions simultaneous with a quantification of transcriptional activity and identity of the community. We highlight the patterns of eukaryotic gene expression for 31 biogeochemically significant gene targets hypothesized to be valuable within forecasting models. An advantage to this targeted approach is that the database of reference sequences used to identify the target genes was selectively constructed and highly curated optimizing taxonomic coverage, throughput, and the accuracy of annotations. A coastal diatom bloom highly expressed nitrate transporters and carbonic anhydrase presumably to support high growth rates and enhance uptake of low levels of dissolved nitrate and CO2. Diatom-diazotroph association (DDA: diatoms with nitrogen fixing symbionts) blooms were common when surface salinity was mesohaline and dissolved nitrate concentrations were below detection, and hence did not show evidence of nitrate utilization, suggesting they relied on ammonium transporters to aquire recently fixed nitrogen. These DDA blooms in the outer plume had rapid turnover of the photosystem D1 protein presumably caused by photodegradation under increased light penetration in clearer waters, and increased expression of silicon transporters as silicon became limiting. Expression of these genes, including carbonic anhydrase and transporters for nitrate and phosphate, were found to reflect the physiological status and biogeochemistry of river plume environments. These relatively stable patterns of eukaryotic transcript abundance occurred over modest spatiotemporal scales, with similarity observed in sample duplicates collected up to 2.45 km in space and 120 minutes in time. These results confirm the use of metatranscriptomics as a valuable tool to understand and predict microbial community function.", "link"=>"http://www.mendeley.com/research/patterns-transcript-abundance-eukaryotic-biogeochemicallyrelevant-genes-amazon-river-plume", "reader_count"=>27, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>3, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>17, "Business, Management and Accounting"=>1, "Earth and Planetary Sciences"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>3}}, "reader_count_by_country"=>{"Japan"=>1, "Egypt"=>1}, "group_count"=>3}

Scopus | Further Information

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