Cellular Functions and Gene and Protein Expression Profiles in Endothelial Cells Derived from Moyamoya Disease-Specific iPS Cells
Publication Date
September 23, 2016
Journal
PLOS ONE
Authors
Shuji Hamauchi, Hideo Shichinohe, Haruto Uchino, Shigeru Yamaguchi, et al
Volume
11
Issue
9
Pages
e0163561
DOI
https://dx.plos.org/10.1371/journal.pone.0163561
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0163561
Scopus
84992073966
Mendeley
http://www.mendeley.com/research/cellular-functions-gene-protein-expression-profiles-endothelial-cells-derived-moyamoya-diseasespecif
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Mendeley | Further Information

{"title"=>"Cellular functions and gene and protein expression profiles in endothelial cells derived from Moyamoya disease-specific iPS cells", "type"=>"journal", "authors"=>[{"first_name"=>"Shuji", "last_name"=>"Hamauchi", "scopus_author_id"=>"36238193000"}, {"first_name"=>"Hideo", "last_name"=>"Shichinohe", "scopus_author_id"=>"6602249374"}, {"first_name"=>"Haruto", "last_name"=>"Uchino", "scopus_author_id"=>"55212681000"}, {"first_name"=>"Shigeru", "last_name"=>"Yamaguchi", "scopus_author_id"=>"36465916100"}, {"first_name"=>"Naoki", "last_name"=>"Nakayama", "scopus_author_id"=>"7102488258"}, {"first_name"=>"Ken", "last_name"=>"Kazumata", "scopus_author_id"=>"6701760851"}, {"first_name"=>"Toshiya", "last_name"=>"Osanai", "scopus_author_id"=>"7005085216"}, {"first_name"=>"Takeo", "last_name"=>"Abumiya", "scopus_author_id"=>"6701341284"}, {"first_name"=>"Kiyohiro", "last_name"=>"Houkin", "scopus_author_id"=>"36786354100"}, {"first_name"=>"Takumi", "last_name"=>"Era", "scopus_author_id"=>"6701576515"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0163561", "sgr"=>"84992073966", "pui"=>"612774861", "scopus"=>"2-s2.0-84992073966", "issn"=>"19326203", "pmid"=>"27662211"}, "id"=>"9662e4b6-04b8-3f38-9be8-8826bf4b518a", "abstract"=>"BACKGROUND AND PURPOSE: Moyamoya disease (MMD) is a slow, progressive steno-occlusive disease, arising in the terminal portions of the cerebral internal carotid artery. However, the functions and characteristics of the endothelial cells (ECs) in MMD are unknown. We analyzed these features using induced pluripotent stem cell (iPSC)-derived ECs.\\n\\nMETHODS: iPSC lines were established from the peripheral blood of three patients with MMD carrying the variant RNF213 R4810K, and three healthy persons used as controls. After the endothelial differentiation of iPSCs, CD31+CD144+ cells were purified as ECs using a cell sorter. We analyzed their proliferation, angiogenesis, and responses to some angiogenic factors, namely VEGF, bFGF, TGF-β, and BMP4. The ECs were also analyzed using DNA microarray and proteomics to perform comprehensive gene and protein expression analysis.\\n\\nRESULTS: Angiogenesis was significantly impaired in MMD regardless of the presence of any angiogenic factor. On the contrary, endothelial proliferation was not significant between control- and MMD-derived cells. Regarding DNA microarray, pathway analysis illustrated that extracellular matrix (ECM) receptor-related genes, including integrin β3, were significantly downregulated in MMD. Proteomic analysis revealed that cytoskeleton-related proteins were downregulated and splicing regulation-related proteins were upregulated in MMD.\\n\\nCONCLUSIONS: Downregulation of ECM receptor-related genes may be associated with impaired angiogenic activity in ECs derived from iPSCs from patients with MMD. Upregulation of splicing regulation-related proteins implied differences in splicing patterns between control and MMD ECs.", "link"=>"http://www.mendeley.com/research/cellular-functions-gene-protein-expression-profiles-endothelial-cells-derived-moyamoya-diseasespecif", "reader_count"=>11, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>5, "Agricultural and Biological Sciences"=>1, "Neuroscience"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Neuroscience"=>{"Neuroscience"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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