Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles
Publication Date
September 29, 2016
Journal
PLOS ONE
Authors
Stefanie Fischer, Kerstin Cornils, Thomas Speiseder, Anita Badbaran, et al
Volume
11
Issue
9
Pages
e0163665
DOI
https://dx.plos.org/10.1371/journal.pone.0163665
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0163665
Scopus
84991670969
Mendeley
http://www.mendeley.com/research/indication-horizontal-dna-gene-transfer-extracellular-vesicles
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Mendeley | Further Information

{"title"=>"Indication of horizontal DNA gene transfer by extracellular vesicles", "type"=>"journal", "authors"=>[{"first_name"=>"Stefanie", "last_name"=>"Fischer", "scopus_author_id"=>"57191581290"}, {"first_name"=>"Kerstin", "last_name"=>"Cornils", "scopus_author_id"=>"12779954800"}, {"first_name"=>"Thomas", "last_name"=>"Speiseder", "scopus_author_id"=>"23768368500"}, {"first_name"=>"Anita", "last_name"=>"Badbaran", "scopus_author_id"=>"6507097302"}, {"first_name"=>"Rudolph", "last_name"=>"Reimer", "scopus_author_id"=>"22836307100"}, {"first_name"=>"Daniela", "last_name"=>"Indenbirken", "scopus_author_id"=>"56167538100"}, {"first_name"=>"Adam", "last_name"=>"Grundhoff", "scopus_author_id"=>"6508081008"}, {"first_name"=>"Bärbel", "last_name"=>"Brunswig-Spickenheier", "scopus_author_id"=>"6603521509"}, {"first_name"=>"Malik", "last_name"=>"Alawi", "scopus_author_id"=>"55818873100"}, {"first_name"=>"Claudia", "last_name"=>"Lange", "scopus_author_id"=>"35071550000"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0163665", "sgr"=>"84991670969", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pmid"=>"27684368", "issn"=>"19326203", "scopus"=>"2-s2.0-84991670969", "pui"=>"612795372"}, "id"=>"83a16f30-c34f-391d-918a-67faa236a149", "abstract"=>"Abstract The biological relevance of extracellular vesicles (EV) in intercellular communication has been well established. Thus far, proteins and RNA were described as main cargo. Here, we show that EV released from human bone marrow derived mesenchymal stromal cells (BM- hMSC) also carry high-molecular DNA in addition. Extensive EV characterization revealed this DNA mainly associated with the outer EV membrane and to a smaller degree also inside the EV. Our EV purification protocol secured that DNA is not derived from apoptotic or necrotic cells. To analyze the relevance of EV-associated DNA we lentivirally transduced Arabidopsis thaliana-DNA (A.t.-DNA) as indicator into BM-hMSC and generated EV. Using quantitative polymerase chain reaction (qPCR) techniques we detected high copy numbers of A.t.-DNA in EV. In recipient hMSC incubated with tagged EV for two weeks we identified A.t.-DNA transferred to recipient cells. Investigation of recipient cell DNA using quantitative PCR and verification of PCR-products by sequencing suggested stable integration of A.t.- DNA. In conclusion, for the first time our proof-of-principle experiments point to horizontal DNA transfer into recipient cells via EV. Based on our results we assume that eukaryotic cells are able to exchange genetic information in form of DNA extending the known cargo of EV by genomic DNA. This mechanism might be of relevance in cancer but also during cell evolution and development.", "link"=>"http://www.mendeley.com/research/indication-horizontal-dna-gene-transfer-extracellular-vesicles", "reader_count"=>31, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Master"=>8, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Master"=>8, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>9, "Medicine and Dentistry"=>5, "Agricultural and Biological Sciences"=>12, "Chemistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>9}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Austria"=>1, "United States"=>1, "Germany"=>1}, "group_count"=>0}

Scopus | Further Information

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