The Effect of Albumin on MRP2 and BCRP in the Vesicular Transport Assay
Publication Date
October 05, 2016
Journal
PLOS ONE
Authors
Feng Deng, Noora Sjöstedt & Heidi Kidron
Volume
11
Issue
10
Pages
e0163886
DOI
https://dx.plos.org/10.1371/journal.pone.0163886
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0163886
Scopus
84992163507
Mendeley
http://www.mendeley.com/research/effect-albumin-mrp2-bcrp-vesicular-transport-assay
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Mendeley | Further Information

{"title"=>"The effect of albumin on MRP2 and BCRP in the vesicular transport assay", "type"=>"journal", "authors"=>[{"first_name"=>"Feng", "last_name"=>"Deng", "scopus_author_id"=>"57190256821"}, {"first_name"=>"Noora", "last_name"=>"Sjöstedt", "scopus_author_id"=>"55984415500"}, {"first_name"=>"Heidi", "last_name"=>"Kidron", "scopus_author_id"=>"7801447472"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "doi"=>"10.1371/journal.pone.0163886", "sgr"=>"84992163507", "scopus"=>"2-s2.0-84992163507", "pmid"=>"27706255", "pui"=>"612644012"}, "id"=>"b839820f-ed7a-39f4-ba48-5f08212f1172", "abstract"=>"The ABC transporters multidrug resistance associated protein 2 (MRP2) and breast cancer resistance protein (BCRP) are of interest in drug development, since they affect the pharmacokinetics of several drugs. Membrane vesicle transport assays are widely used to study interactions with these proteins. Since albumin has been found to affect the kinetics of metabolic enzymes in similar membrane preparations, we investigated whether albumin affects the kinetic parameters of efflux transport. We found that albumin increased the Vmax of 5(6)-carboxy-2',7'-dichlorofluorescein (CDCF) and estradiol-17-β-D-glucuronide uptake into MRP2 vesicles in the presence of 0.1% bovine serum albumin (BSA) by 2 and 1.5-fold, respectively, while BSA increased Lucifer yellow uptake by 30% in BCRP vesicles. Km values increased slightly, but the change was not statistically significant. The effect of BSA on substrate uptake was dependent on the vesicle amount, while increasing BSA concentration did not significantly improve substrate uptake. These results indicate a minor effect of albumin on MRP2 and BCRP, but it should be considered if albumin is added to transporter assays for example as a solubilizer, since the effect may be substrate or transporter specific.", "link"=>"http://www.mendeley.com/research/effect-albumin-mrp2-bcrp-vesicular-transport-assay", "reader_count"=>7, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Master"=>4, "Other"=>1, "Researcher"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Master"=>4, "Other"=>1, "Researcher"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "reader_count_by_country"=>{"Ghana"=>1}, "group_count"=>0}

Scopus | Further Information

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