In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors
Publication Date
October 12, 2016
Journal
PLOS ONE
Authors
Ramiro Olivera, Lucia Natalia Moro, Roberto Jordan, Carlos Luzzani, et al
Volume
11
Issue
10
Pages
e0164049
DOI
https://dx.plos.org/10.1371/journal.pone.0164049
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0164049
Scopus
84991454565
Mendeley
http://www.mendeley.com/research/vitro-vivo-development-horse-cloned-embryos-generated-ipscs-mesenchymal-stromal-cells-fetal-adult-fi
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Mendeley | Further Information

{"title"=>"In Vitro and in vivo development of horse cloned embryos generated with iPSCs, mesenchymal stromal cells and fetal or adult fibroblasts as nuclear donors", "type"=>"journal", "authors"=>[{"first_name"=>"Ramiro", "last_name"=>"Olivera", "scopus_author_id"=>"24829675000"}, {"first_name"=>"Lucia Natalia", "last_name"=>"Moro", "scopus_author_id"=>"55457277100"}, {"first_name"=>"Roberto", "last_name"=>"Jordan", "scopus_author_id"=>"57191536031"}, {"first_name"=>"Carlos", "last_name"=>"Luzzani", "scopus_author_id"=>"23012694700"}, {"first_name"=>"Santiago", "last_name"=>"Miriuka", "scopus_author_id"=>"6506986052"}, {"first_name"=>"Martin", "last_name"=>"Radrizzani", "scopus_author_id"=>"6701826049"}, {"first_name"=>"F.", "last_name"=>"Xavier Donadeu", "scopus_author_id"=>"57191537532"}, {"first_name"=>"Gabriel", "last_name"=>"Vichera", "scopus_author_id"=>"24829707500"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"612655821", "scopus"=>"2-s2.0-84991454565", "doi"=>"10.1371/journal.pone.0164049", "sgr"=>"84991454565"}, "id"=>"5e8e92b9-da45-3aac-9473-2083b9891e09", "abstract"=>"The demand for equine cloning as a tool to preserve high genetic value is growing world-wide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (<1h, n = 1261; 1-2h, n = 1773; 2-3h, n = 1647) on in vitro and in vivo development of equine embryos generated by cloning. Then, we evaluated the effects of using different nuclear donor cell types in two successive experiments: I) induced pluripotent stem cells (iPSCs) vs. adult fibroblasts (AF) fused to ooplasts injected with the pluripotency-inducing genes OCT4, SOX2, MYC and KLF4, vs. AF alone as controls; II) umbilical cord-derived mesenchymal stromal cells (UC-MSCs) vs. fetal fibroblasts derived from an unborn cloned foetus (FF) vs. AF from the original individ-ual. In the first experiment, both blastocyst production and pregnancy rates were higher in the 2-3h group (11.5% and 9.5%, respectively), respect to <1h (5.2% and 2%, respectively) and 1-2h (5.6% and 4.7%, respectively) groups (P<0.05). However, percentages of born foals/pregnancies were similar when intervals of 2-3h (35.2%) or 1-2h (35.7%) were used. In contrast to AF, the iPSCs did not generate any blastocyst-stage embryos. Moreover, injection of oocytes with the pluripotency-inducing genes did not improve blastocyst pro-duction nor pregnancy rates respect to AF controls. Finally, higher blastocyst production was obtained using UC-MSC (15.6%) than using FF (8.9%) or AF (9.3%), (P<0.05). Despite pregnancy rates were similar for these 3 groups (17.6%, 18.2% and 22%, respec-tively), viable foals (two) were obtained only by using FF. In summary, optimum blastocyst production rates can be obtained using a 2-3h interval between cell fusion and activation as well as using UC-MSCs as nuclear donors. Moreover, FF line can improve the efficiency of an inefficient AF line. Overall, 24 healthy foals were obtained from a total of 29 born foals.", "link"=>"http://www.mendeley.com/research/vitro-vivo-development-horse-cloned-embryos-generated-ipscs-mesenchymal-stromal-cells-fetal-adult-fi", "reader_count"=>9, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>5, "Veterinary Science and Veterinary Medicine"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

Scopus | Further Information

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