Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria
Publication Date
October 05, 2016
Journal
PLOS ONE
Authors
Areli Herrera Diaz, Izabella Kovacs & Christian Lindermayr
Volume
11
Issue
10
Pages
e0164097
DOI
https://dx.plos.org/10.1371/journal.pone.0164097
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0164097
Scopus
84991263393
Mendeley
http://www.mendeley.com/research/inducible-expression-denovo-designed-antimicrobial-peptide-sp11-tomato-confers-resistance-xanthomona
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Mendeley | Further Information

{"title"=>"Inducible expression of the De-novo designed antimicrobial peptide SP1-1 in tomato confers resistance to Xanthomonas campestris pv. Vesicatoria", "type"=>"journal", "authors"=>[{"first_name"=>"Areli Herrera", "last_name"=>"Diaz", "scopus_author_id"=>"56415735900"}, {"first_name"=>"Izabella", "last_name"=>"Kovacs", "scopus_author_id"=>"57196858782"}, {"first_name"=>"Christian", "last_name"=>"Lindermayr", "scopus_author_id"=>"8614856000"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27706237", "scopus"=>"2-s2.0-84991263393", "pui"=>"612643932", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0164097", "sgr"=>"84991263393"}, "id"=>"bebd2e08-b1e6-318d-b1e9-2e8dd01eb249", "abstract"=>"Antimicrobial peptides (AMPs) are small peptides with less than 50 amino acids and are part of the innate immune response in almost all organisms, including bacteria, vertebrates, invertebrates and plants. AMPs are active against a broad-spectrum of pathogens. The inducible expression of AMPs in plants is a promising approach to combat plant pathogens with minimal negative side effects, such as phytotoxicity or infertility. In this study, inducible expression of the de-novo designed AMP SP1-1 in Micro Tom tomato protected tomato fruits against bacterial spot disease caused by Xanthomonas campestris pv. vesicatoria. The peptide SP1-1 was targeted to the apoplast which is the primary infection site for plant pathogens, by fusing SP1-1 peptide to the signal peptide RsAFP1 of radish (Raphanus sativus). The pathogen inducibility of the expression was enabled by using an optimized inducible 4XW2/4XS promoter. As a result, the tomato fruits of independently generated SP1-1 transgenic lines were significantly more resistant to X. campestris pv. vesicatoria than WT tomato fruits. In transgenic lines, bacterial infection was reduced up to 65% in comparison to the infection of WT plants. Our study demonstrates that the combination of the 4XW2/4XS cis-element from parsley with the synthetic antimicrobial peptide SP1-1 is a good alternative to protect tomato fruits against infections with X. campestris pv. vesicatoria.", "link"=>"http://www.mendeley.com/research/inducible-expression-denovo-designed-antimicrobial-peptide-sp11-tomato-confers-resistance-xanthomona", "reader_count"=>9, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>2, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>2, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"France"=>1}, "group_count"=>1}

Scopus | Further Information

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