Genetically Engineered Escherichia coli Nissle 1917 Synbiotics Reduce Metabolic Effects Induced by Chronic Consumption of Dietary Fructose
Publication Date
October 19, 2016
Journal
PLOS ONE
Authors
Chaudhari Archana Somabhai, Ruma Raghuvanshi & G. Nareshkumar
Volume
11
Issue
10
Pages
e0164860
DOI
https://dx.plos.org/10.1371/journal.pone.0164860
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0164860
Scopus
84992529844
Mendeley
http://www.mendeley.com/research/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-chro
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Mendeley | Further Information

{"title"=>"Genetically engineered Escherichia coli Nissle 1917 Synbiotics reduce metabolic effects induced by chronic consumption of dietary fructose", "type"=>"journal", "authors"=>[{"first_name"=>"Chaudhari Archana", "last_name"=>"Somabhai", "scopus_author_id"=>"57191710589"}, {"first_name"=>"Ruma", "last_name"=>"Raghuvanshi", "scopus_author_id"=>"57189345872"}, {"first_name"=>"G.", "last_name"=>"Nareshkumar", "scopus_author_id"=>"57191605677"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84992529844", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0164860", "pmid"=>"27760187", "pui"=>"612830951", "scopus"=>"2-s2.0-84992529844", "isbn"=>"1932-6203"}, "id"=>"90b6def6-947e-3358-ae6e-175541213f6a", "abstract"=>"Aims To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. Materials and Methods EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles foster rats weighing 150–200 g were fed with 20% fructose in drinking water for two months. Probiotic treatment of EcN (pqq), EcN (pqq-glf-mtlK), EcN (pqq-fdh) was given once per week 10 9 cells for two months. Furthermore, blood and liver parameters for oxidative stress, dyslipidemia and hyperglycemia were estimated. Fecal samples were collected to determine the production of short chain fatty acids and pyrrolo-quinoline quinone (PQQ) production. Results EcN (pqq-glf-mtlK), EcN (pqq-fdh) transformants were confirmed by restriction digestion and functionality was checked by PQQ estimation and HPLC analysis. There was signifi-cant increase in body weight, serum glucose, liver injury markers, lipid profile in serum and liver, and decrease in antioxidant enzyme activity in high-fructose-fed rats. However the rats treated with EcN (pqq-glf-mtlK) and EcN (pqq-fdh) showed significant reduction in lipid peroxidation along with increase in serum and hepatic antioxidant enzyme activities. Resto-ration of liver injury marker enzymes was also seen. Increase in short chain fatty acids (SCFA) demonstrated the prebiotic effects of mannitol and gluconic acid. Abbreviations: EcN, Escherichia coli Nissle 1917; TG, Triglycerides; Fdh, Fructose dehydrogenase; Conclusions Our study demonstrated the effectiveness of probiotic EcN producing PQQ and fructose metabolizing enzymes against the fructose induced hepatic steatosis suggesting that its potential for use in treating fructose induced metabolic syndrome.", "link"=>"http://www.mendeley.com/research/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-chro", "reader_count"=>11, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

Scopus | Further Information

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