Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes
Publication Date
November 16, 2016
Authors
Philip Francis Thomsen, Peter Rask Møller, Eva Egelyng Sigsgaard, Steen Wilhelm Knudsen, et al
Volume
11
Issue
11
Pages
e0165252
DOI
https://dx.plos.org/10.1371/journal.pone.0165252
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0165252
Web of Science
000387909300012
Scopus
84995610967
Mendeley
http://www.mendeley.com/research/environmental-dna-seawater-samples-correlate-trawl-catches-subarctic-deepwater-fishes
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{"title"=>"Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes", "type"=>"journal", "authors"=>[{"first_name"=>"Philip Francis", "last_name"=>"Thomsen", "scopus_author_id"=>"8392194400"}, {"first_name"=>"Peter Rask", "last_name"=>"Møller", "scopus_author_id"=>"57193746771"}, {"first_name"=>"Eva Egelyng", "last_name"=>"Sigsgaard", "scopus_author_id"=>"56455229500"}, {"first_name"=>"Steen Wilhelm", "last_name"=>"Knudsen", "scopus_author_id"=>"16836501300"}, {"first_name"=>"Ole Ankjær", "last_name"=>"Jørgensen", "scopus_author_id"=>"16420632700"}, {"first_name"=>"Eske", "last_name"=>"Willerslev", "scopus_author_id"=>"56273625000"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"613225447", "issn"=>"19326203", "isbn"=>"1932-6203; 1932-6203", "doi"=>"10.1371/journal.pone.0165252", "scopus"=>"2-s2.0-84995610967", "pmid"=>"27851757", "sgr"=>"84995610967"}, "id"=>"25e68c64-624e-3426-9fc8-41fad09d43ca", "abstract"=>"Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope depths in Southwest Greenland. We collected seawater samples at depths of 188–918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites showed good correspondence with catch sizes. Environmental DNA sequence reads from the fish assemblages correlated with biomass and abundance data obtained from trawling. Interestingly, the Greenland shark (Somniosus microcephalus) showed high abundance of eDNA reads despite only a single specimen being caught, demonstrating the relevance of the eDNA approach for large species that can probably avoid bottom trawls in most cases. Quantitative detection of marine fish using eDNA remains to be tested further to ascertain whether this technique is able to yield credible results for routine application in fisheries. Nevertheless, our study demonstrates that eDNA reads can be used as a qualitative and quantitative proxy for marine fish assemblages in deepwater oceanic habitats. This relates directly to applied fisheries as well as to monitoring effects of ongoing climate change on marine biodiversity—especially in polar ecosystems.", "link"=>"http://www.mendeley.com/research/environmental-dna-seawater-samples-correlate-trawl-catches-subarctic-deepwater-fishes", "reader_count"=>216, "reader_count_by_academic_status"=>{"Unspecified"=>10, "Professor > Associate Professor"=>5, "Student > Doctoral Student"=>6, "Researcher"=>53, "Student > Ph. D. Student"=>54, "Student > Postgraduate"=>10, "Student > Master"=>41, "Other"=>12, "Student > Bachelor"=>18, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>10, "Professor > Associate Professor"=>5, "Student > Doctoral Student"=>6, "Researcher"=>53, "Student > Ph. D. Student"=>54, "Student > Postgraduate"=>10, "Student > Master"=>41, "Other"=>12, "Student > Bachelor"=>18, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>15, "Engineering"=>2, "Environmental Science"=>45, "Biochemistry, Genetics and Molecular Biology"=>34, "Agricultural and Biological Sciences"=>108, "Medicine and Dentistry"=>1, "Arts and Humanities"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Business, Management and Accounting"=>1, "Immunology and Microbiology"=>1, "Earth and Planetary Sciences"=>5, "Economics, Econometrics and Finance"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>5}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>108}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>34}, "Unspecified"=>{"Unspecified"=>15}, "Environmental Science"=>{"Environmental Science"=>45}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>1, "Ireland"=>1, "Brazil"=>1, "South Africa"=>2, "United Kingdom"=>1, "Australia"=>1, "Chile"=>1}, "group_count"=>12}

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Scopus | Further Information

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