Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion
Publication Date
January 06, 2017
Authors
Sophie Laget, Lucile Broncy, Katia Hormigos, Dalia M. Dhingra, et al
Volume
12
Issue
1
Pages
e0169427
DOI
https://dx.plos.org/10.1371/journal.pone.0169427
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0169427
Scopus
85009062659
Mendeley
http://www.mendeley.com/research/technical-insights-highly-sensitive-isolation-molecular-characterization-fixed-live-circulating-tumo-2
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Technical insights into highly sensitive isolation and molecular characterization of fixed and live circulating tumor cells for early detection of tumor invasion", "type"=>"journal", "authors"=>[{"first_name"=>"Sophie", "last_name"=>"Laget", "scopus_author_id"=>"22941758400"}, {"first_name"=>"Lucile", "last_name"=>"Broncy", "scopus_author_id"=>"57056413800"}, {"first_name"=>"Katia", "last_name"=>"Hormigos", "scopus_author_id"=>"57195362530"}, {"first_name"=>"Dalia M.", "last_name"=>"Dhingra", "scopus_author_id"=>"57192915772"}, {"first_name"=>"Fatima", "last_name"=>"BenMohamed", "scopus_author_id"=>"24921215900"}, {"first_name"=>"Thierry", "last_name"=>"Capiod", "scopus_author_id"=>"7003324059"}, {"first_name"=>"Magne", "last_name"=>"Osteras", "scopus_author_id"=>"6603944641"}, {"first_name"=>"Laurent", "last_name"=>"Farinelli", "scopus_author_id"=>"35247957600"}, {"first_name"=>"Stephen", "last_name"=>"Jackson", "scopus_author_id"=>"57022863400"}, {"first_name"=>"Patrizia", "last_name"=>"Paterlini-Bréchot", "scopus_author_id"=>"6603876883"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1111111111", "sgr"=>"85009062659", "scopus"=>"2-s2.0-85009062659", "issn"=>"19326203", "pui"=>"614015467", "doi"=>"10.1371/journal.pone.0169427", "pmid"=>"28060956"}, "id"=>"dc62268b-32c3-32ce-a94b-8fe497490ba8", "abstract"=>"Circulating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET® (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET® in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion.", "link"=>"http://www.mendeley.com/research/technical-insights-highly-sensitive-isolation-molecular-characterization-fixed-live-circulating-tumo-2", "reader_count"=>38, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>4, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>4, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>7, "Biochemistry, Genetics and Molecular Biology"=>9, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>5, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>3, "Social Sciences"=>1, "Computer Science"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>7}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Chemistry"=>{"Chemistry"=>3}, "Social Sciences"=>{"Social Sciences"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>9}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Italy"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85009062659"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85009062659?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85009062659&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85009062659&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85009062659", "dc:identifier"=>"SCOPUS_ID:85009062659", "eid"=>"2-s2.0-85009062659", "dc:title"=>"Technical insights into highly sensitive isolation and molecular characterization of fixed and live circulating tumor cells for early detection of tumor invasion", "dc:creator"=>"Laget S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"12", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-01-01", "prism:coverDisplayDate"=>"January 2017", "prism:doi"=>"10.1371/journal.pone.0169427", "citedby-count"=>"14", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Rarecells Diagnostics", "affiliation-city"=>"Paris", "affiliation-country"=>"France"}], "pubmed-id"=>"28060956", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0169427", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"325", "xml_views"=>"5", "html_views"=>"611"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"143", "xml_views"=>"2", "html_views"=>"478"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"75", "xml_views"=>"2", "html_views"=>"175"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"114", "xml_views"=>"6", "html_views"=>"299"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"63", "xml_views"=>"1", "html_views"=>"251"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"37", "xml_views"=>"1", "html_views"=>"178"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"55", "xml_views"=>"2", "html_views"=>"130"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"89"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"31", "xml_views"=>"2", "html_views"=>"95"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"1", "html_views"=>"201"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"4", "html_views"=>"346"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"49", "xml_views"=>"2", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"45", "xml_views"=>"13", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"37", "xml_views"=>"6", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"34", "xml_views"=>"22", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"42", "xml_views"=>"16", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"46", "xml_views"=>"2", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"46", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"48", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"39", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"17"}

PMC Usage Stats

  • {"unique-ip"=>"79", "full-text"=>"82", "pdf"=>"30", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"29", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"165", "full-text"=>"163", "pdf"=>"75", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"101", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"108", "full-text"=>"122", "pdf"=>"56", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"68", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"261", "full-text"=>"260", "pdf"=>"94", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"125", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"144", "full-text"=>"151", "pdf"=>"53", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"104", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"67", "full-text"=>"83", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"42", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"74", "full-text"=>"91", "pdf"=>"49", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"57", "full-text"=>"76", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"80", "full-text"=>"69", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"34", "supp-data"=>"9", "cited-by"=>"1", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"57", "full-text"=>"68", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"51", "full-text"=>"61", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"27", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"40", "full-text"=>"53", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"44", "full-text"=>"50", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"26", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"50", "full-text"=>"55", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"40", "full-text"=>"48", "pdf"=>"19", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"43", "full-text"=>"46", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"51", "full-text"=>"44", "pdf"=>"13", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"32", "full-text"=>"54", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"45", "full-text"=>"58", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"41", "full-text"=>"41", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"40", "full-text"=>"38", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"32", "full-text"=>"32", "pdf"=>"17", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"32", "full-text"=>"31", "pdf"=>"13", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"42", "full-text"=>"42", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"32", "full-text"=>"36", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"37", "full-text"=>"40", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"29", "full-text"=>"31", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"13", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
Loading … Spinner
There are currently no alerts