Drawbacks of Dialysis Procedures for Removal of EDTA
Publication Date
January 18, 2017
Authors
Andreia Mónico, Eva Martínez Senra, F. Javier Cañada, Silvia Zorrilla, et al
Volume
12
Issue
1
Pages
e0169843
DOI
https://dx.plos.org/10.1371/journal.pone.0169843
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0169843
Scopus
85009889687
Mendeley
http://www.mendeley.com/research/drawbacks-dialysis-procedures-removal-edta
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Drawbacks of dialysis procedures for removal of EDTA", "type"=>"journal", "authors"=>[{"first_name"=>"Andreia", "last_name"=>"Mónico", "scopus_author_id"=>"57193601180"}, {"first_name"=>"Eva", "last_name"=>"Martínez-Senra", "scopus_author_id"=>"57193000948"}, {"first_name"=>"F. Javier", "last_name"=>"Cañada", "scopus_author_id"=>"7004569351"}, {"first_name"=>"Silvia", "last_name"=>"Zorrilla", "scopus_author_id"=>"6701838413"}, {"first_name"=>"Dolores", "last_name"=>"Pérez-Sala", "scopus_author_id"=>"7004092646"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"614108960", "sgr"=>"85009889687", "issn"=>"19326203", "pmid"=>"28099451", "scopus"=>"2-s2.0-85009889687", "doi"=>"10.1371/journal.pone.0169843", "isbn"=>"1111111111"}, "id"=>"ee51b7ae-b2b9-30d2-b4f0-3bc02e89f134", "abstract"=>"© 2017 MacPherson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Ethylenediaminetetraacetic acid (EDTA) is a chelating agent commonly used in protein purification, both to eliminate contaminating divalent cations and to inhibit protease activity. For a number of subsequent applications EDTA needs to be exhaustively removed. Most purification methods rely in extensive dialysis and/or gel filtration in order to exchange or remove protein buffer components, including metal chelators. We report here that dialysis protocols, even as extensive as those typically employed for protein refolding, may not effectively remove EDTA, which is reduced only by approximately two-fold and it also persists after spin-column gel filtration, as determined by NMR and by colorimetric methods. Remarkably, the most efficient removal was achieved by ultrafiltration, after which EDTA became virtually undetectable. These results highlight a potentially widespread source of experimental variability affecting free divalent cation concentrations in protein applications.", "link"=>"http://www.mendeley.com/research/drawbacks-dialysis-procedures-removal-edta", "reader_count"=>11, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Bachelor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Bachelor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85009889687"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85009889687?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85009889687&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85009889687&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85009889687", "dc:identifier"=>"SCOPUS_ID:85009889687", "eid"=>"2-s2.0-85009889687", "dc:title"=>"Drawbacks of dialysis procedures for removal of EDTA", "dc:creator"=>"Mónico A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"12", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-01-01", "prism:coverDisplayDate"=>"January 2017", "prism:doi"=>"10.1371/journal.pone.0169843", "citedby-count"=>"6", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"CSIC - Centro de Investigaciones Biologicas (CIB)", "affiliation-city"=>"Madrid", "affiliation-country"=>"Spain"}], "pubmed-id"=>"28099451", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0169843", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"5", "html_views"=>"117"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"2", "html_views"=>"107"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"51", "xml_views"=>"3", "html_views"=>"283"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"165"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"178"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"170"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"136"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"121"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"197"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"248"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"27", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"97"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"68"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"34", "xml_views"=>"2", "html_views"=>"73"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"55"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"76"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"60"}

PMC Usage Stats

  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"27", "full-text"=>"30", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"33", "full-text"=>"33", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"38", "full-text"=>"39", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"33", "full-text"=>"31", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"54", "full-text"=>"58", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"53", "full-text"=>"63", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"65", "full-text"=>"70", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"34", "full-text"=>"35", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"36", "full-text"=>"43", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"66", "full-text"=>"72", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"40", "full-text"=>"45", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"54", "full-text"=>"57", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"64", "full-text"=>"74", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"48", "full-text"=>"50", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"36", "full-text"=>"37", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"43", "full-text"=>"48", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"59", "full-text"=>"64", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"63", "full-text"=>"69", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"52", "full-text"=>"55", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"48", "full-text"=>"53", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"55", "full-text"=>"61", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"61", "full-text"=>"65", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"78", "full-text"=>"85", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
Loading … Spinner
There are currently no alerts