Polymicrobial Ventilator-Associated Pneumonia: Fighting In Vitro Candida albicans-Pseudomonas aeruginosa Biofilms with Antifungal-Antibacterial Combination Therapy
Publication Date
January 23, 2017
Authors
Maria E. Rodrigues, Susana P. Lopes, Cláudia R. Pereira, Nuno F. Azevedo, et al
Volume
12
Issue
1
Pages
e0170433
DOI
https://dx.plos.org/10.1371/journal.pone.0170433
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0170433
Scopus
85010434693
Mendeley
http://www.mendeley.com/research/polymicrobial-ventilatorassociated-pneumonia-fighting-vitro-candida-albicanspseudomonas-aeruginosa-b
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Mendeley | Further Information

{"title"=>"Polymicrobial ventilator-associated pneumonia: Fighting in vitro Candida albicans-pseudomonas aeruginosa biofilms with antifungal-antibacterial combination therapy", "type"=>"journal", "authors"=>[{"first_name"=>"Maria E.", "last_name"=>"Rodrigues", "scopus_author_id"=>"35215891900"}, {"first_name"=>"Susana P.", "last_name"=>"Lopes", "scopus_author_id"=>"36908262200"}, {"first_name"=>"Cl�udia R.", "last_name"=>"Pereira", "scopus_author_id"=>"57201166722"}, {"first_name"=>"Nuno F.", "last_name"=>"Azevedo", "scopus_author_id"=>"8092231700"}, {"first_name"=>"An�lia", "last_name"=>"Louren�o", "scopus_author_id"=>"57201107040"}, {"first_name"=>"Mariana", "last_name"=>"Henriques", "scopus_author_id"=>"7006665006"}, {"first_name"=>"Maria O.", "last_name"=>"Pereira", "scopus_author_id"=>"7401842375"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85010434693", "doi"=>"10.1371/journal.pone.0170433", "pui"=>"614168737", "pmid"=>"28114348", "scopus"=>"2-s2.0-85010434693", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"eba0d3b2-33f3-34c8-86e1-c42a7491151e", "abstract"=>"The polymicrobial nature of ventilator-associated pneumonia (VAP) is now evident, with mixed bacterial-fungal biofilms colonizing the VAP endotracheal tube (ETT) surface. The microbial interplay within this infection may contribute for enhanced pathogenesis and exert impact towards antimicrobial therapy. Consequently, the high mortality/morbidity rates associated to VAP and the worldwide increase in antibiotic resistance has promoted the search for novel therapeutic strategies to fight VAP polymicrobial infections. Under this scope, this work aimed to assess the activity of mono- vs combinational antimicrobial therapy using one antibiotic (Polymyxin B; PolyB) and one antifungal (Amphotericin B; AmB) agent against polymicrobial biofilms of Pseudomonas aeruginosa and Candida albicans. The action of isolated antimicrobials was firstly evaluated in single- and polymicrobial cultures, with AmB being more effective against C. albicans and PolyB against P. aeruginosa. Mixed planktonic cultures required equal or higher antimicrobial concentrations. In biofilms, only PolyB at relatively high concentrations could reduce P. aeruginosa in both monospecies and polymicrobial populations, with C. albicans displaying only punctual disturbances. PolyB and AmB exhibited a synergistic effect against P. aeruginosa and C. albicans mixed planktonic cultures, but only high doses (256 mg L-1) of PolyB were able to eradicate polymicrobial biofilms, with P. aeruginosa showing loss of cultivability (but not viability) at 2 h post-treatment, whilst C. albicans only started to be inhibited after 14 h. In conclusion, combination therapy involving an antibiotic and an antifungal agent holds an attractive therapeutic option to treat severe bacterial-fungal polymicrobial infections. Nevertheless, optimization of antimicrobial doses and further clinical pharmacokinetics/pharmacodynamics and toxicodynamics studies underpinning the optimal use of these drugs are urgently required to improve therapy effectiveness and avoid reinfection.", "link"=>"http://www.mendeley.com/research/polymicrobial-ventilatorassociated-pneumonia-fighting-vitro-candida-albicanspseudomonas-aeruginosa-b", "reader_count"=>27, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>4, "Student > Master"=>7, "Other"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>4, "Student > Master"=>7, "Other"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Engineering"=>1, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Nursing and Health Professions"=>1, "Medicine and Dentistry"=>8, "Agricultural and Biological Sciences"=>6, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>5}, "Environmental Science"=>{"Environmental Science"=>1}}, "group_count"=>1}

Scopus | Further Information

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