Automated Remote Focusing, Drift Correction, and Photostimulation to Evaluate Structural Plasticity in Dendritic Spines
Publication Date
January 23, 2017
Authors
Michael S. Smirnov, Paul R. Evans, Tavita R. Garrett, Long Yan, et al
Volume
12
Issue
1
Pages
e0170586
DOI
https://dx.plos.org/10.1371/journal.pone.0170586
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0170586
Scopus
85010366640
Mendeley
http://www.mendeley.com/research/automated-remote-focusing-drift-correction-photostimulation-evaluate-structural-plasticity-dendritic
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Automated remote focusing, drift correction, and photostimulation to evaluate structural plasticity in dendritic spines", "type"=>"journal", "authors"=>[{"first_name"=>"Michael S.", "last_name"=>"Smirnov", "scopus_author_id"=>"57193064226"}, {"first_name"=>"Paul R.", "last_name"=>"Evans", "scopus_author_id"=>"55938599300"}, {"first_name"=>"Tavita R.", "last_name"=>"Garrett", "scopus_author_id"=>"57193062633"}, {"first_name"=>"Long", "last_name"=>"Yan", "scopus_author_id"=>"57193069845"}, {"first_name"=>"Ryohei", "last_name"=>"Yasuda", "scopus_author_id"=>"7006070743"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85010366640", "pmid"=>"28114380", "pui"=>"614168778", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "scopus"=>"2-s2.0-85010366640", "doi"=>"10.1371/journal.pone.0170586", "issn"=>"19326203"}, "id"=>"fa04e576-3b8d-34f8-a1e0-268632c2a392", "abstract"=>"Long-term structural plasticity of dendritic spines plays a key role in synaptic plasticity, the cellular basis for learning and memory. The biochemical step is mediated by a complex network of signaling proteins in spines. Two-photon imaging techniques combined with two-photon glutamate uncaging allows researchers to induce and quantify structural plasticity in single dendritic spines. However, this method is laborious and slow, making it unsuitable for high throughput screening of factors necessary for structural plasticity. Here we introduce a MATLAB-based module built for Scanimage to automatically track, image, and stimulate multiple dendritic spines. We implemented an electrically tunable lens in combination with a drift correction algorithm to rapidly and continuously track targeted spines and correct sample movements. With a straightforward user interface to design custom multi-position experiments, we were able to adequately image and produce targeted plasticity in multiple dendritic spines using glutamate uncaging. Our methods are inexpensive, open source, and provides up to a five-fold increase in throughput for quantifying structural plasticity of dendritic spines.", "link"=>"http://www.mendeley.com/research/automated-remote-focusing-drift-correction-photostimulation-evaluate-structural-plasticity-dendritic", "reader_count"=>24, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>5, "Student > Ph. D. Student"=>10, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>5, "Student > Ph. D. Student"=>10, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>1, "Agricultural and Biological Sciences"=>10, "Neuroscience"=>6, "Psychology"=>1, "Computer Science"=>1, "Physics and Astronomy"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Neuroscience"=>{"Neuroscience"=>6}, "Psychology"=>{"Psychology"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Computer Science"=>{"Computer Science"=>1}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"United States"=>1, "Japan"=>1, "Germany"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85010366640"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85010366640?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010366640&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85010366640&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85010366640", "dc:identifier"=>"SCOPUS_ID:85010366640", "eid"=>"2-s2.0-85010366640", "dc:title"=>"Automated remote focusing, drift correction, and photostimulation to evaluate structural plasticity in dendritic spines", "dc:creator"=>"Smirnov M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"12", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-01-01", "prism:coverDisplayDate"=>"January 2017", "prism:doi"=>"10.1371/journal.pone.0170586", "citedby-count"=>"4", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Max Planck Florida Institute for Neuroscience", "affiliation-city"=>"Jupiter", "affiliation-country"=>"United States"}], "pubmed-id"=>"28114380", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0170586", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Twitter

Counter

  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"51", "xml_views"=>"10", "html_views"=>"474"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"149", "xml_views"=>"1", "html_views"=>"481"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"108"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"130"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"80"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"58"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"156"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"181"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"5", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}

PMC Usage Stats

  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"5"}
Loading … Spinner
There are currently no alerts