Cryopreservation of GABAergic Neuronal Precursors for Cell-Based Therapy
Publication Date
January 25, 2017
Authors
Daniel Rodríguez Martínez, María Magdalena Martínez Losa & Manuel Alvarez Dolado
Volume
12
Issue
1
Pages
e0170776
DOI
https://dx.plos.org/10.1371/journal.pone.0170776
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0170776
Scopus
85010850225
Mendeley
http://www.mendeley.com/research/cryopreservation-gabaergic-neuronal-precursors-cellbased-therapy
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Mendeley | Further Information

{"title"=>"Cryopreservation of GABAergic neuronal precursors for cell-based therapy", "type"=>"journal", "authors"=>[{"first_name"=>"Daniel", "last_name"=>"Rodríguez-Martínez", "scopus_author_id"=>"56416290200"}, {"first_name"=>"María Magdalena", "last_name"=>"Martínez-Losa", "scopus_author_id"=>"6507891516"}, {"first_name"=>"Manuel", "last_name"=>"Alvarez-Dolado", "scopus_author_id"=>"6602957606"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"614210126", "doi"=>"10.1371/journal.pone.0170776", "sgr"=>"85010850225", "isbn"=>"1111111111", "pmid"=>"28122047", "scopus"=>"2-s2.0-85010850225"}, "id"=>"e1ea54e9-ee9c-3551-ad17-1f434b52f0e4", "abstract"=>"Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic neuronal progenitors for cell therapy against interneuron-related pathologies. We used 10% Me2SO as cryoprotectant and assessed the effects of cell culture amplification and cellular organization, as in toto explants, neurospheres, or individualized cells, on post-thaw cell viability and retrieval. We confirmed that in toto cryopreservation of MGE explants is an optimal preservation system to keep intact the interneuron precursor properties for cell transplantation, together with a high cell viability (>80%) and yield (>70%). Post-thaw proliferation and self-renewal of the cryopreserved precursors were tested in vitro. In addition, their migration capacity, acquisition of mature neuronal morphology, and potency to differentiate into multiple interneuron subtypes were also confirmed in vivo after transplantation. The results show that the cryopreserved precursor features remained intact and were similar to those immediately transplanted after their dissection from the MGE. We hope this protocol will facilitate the generation of biobanks to obtain a permanent and reliable source of GABAergic precursors for clinical application in cell-based therapies against interneuronopathies.", "link"=>"http://www.mendeley.com/research/cryopreservation-gabaergic-neuronal-precursors-cellbased-therapy", "reader_count"=>13, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>8, "Medicine and Dentistry"=>2, "Design"=>1}, "reader_count_by_subdiscipline"=>{"Design"=>{"Design"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>0}

Scopus | Further Information

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